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Science China(Life Sciences) 2018年第9期61卷 1127-1129页

作者： Li Gao Kai Li Xiaole Qi Yulong Gao Yongqiang Wang Honglei Gao Xiaomei Wang Division of Avian Infectious Diseases State Key Laboratory of Veterinary Biotechnology Harbin Veterinary Research Institute the Chinese Academy of Agricultural SciencesHarbin 150001 China Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses Yangzhou 225009 China

Dear Editor,Infectious bursal disease virus （IBDV） causes an economically significant disease of chickens worldwide （Berg,2000）. The molecular basis for the virulence of very virulent IBDV （vvIBDV） is not fully understood. Previous studies have shown that genome segment A, especically VP2 protein, plays the most important role in the tropism and pathogenicity of serotype 1 IBDV （Brandt et al., 2001）. VP2 is,however, unlikely to be the only factor for the virulence of vvIBDV （Boot et al., 2000）.

关键词： n-terminal domain of the RnA polymerase of very virulent infectious bursal disease virus contributes to viral replication and virulence Figure RnA

Crystallization and preliminary X-ray analysis of SnR 141--An n-terminal fragment of staphylococcal nuclease R

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Chinese Science Bulletin 1997年第11期42卷 957-959页

作者： Ye, S Wan, ZL Zhou, B Jing, GZ Liang, DC CHInESE ACAD SCI INST BIOPHYSSTATE KEY LAB BIOMACROMOLBEIJING 100101PEOPLES R CHINA

STAPHYLOCOCCAL nuclease(Snase A,EC 3.***.4.7),which hydrolyzes the phosphodiesterbond of DnA and RnA,and releases 3’-phosphate mononucleotides and dinucleotides,con-sists of 149 amino acid residues(MW=16 807)without sulfhydryl and disulfide *** A was originally derived from Staphylococcus *** the gene of the enzyme wascloned and inserted into several expression *** crystal structure was detected

关键词： crystallization staphylococcal nuclease R n-terminal fragment.

c-Jun n-terminal kinase 3 deficiency protects axotomized retinal ganglion cells via affecting mitochondria involved apoptosis pathway

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International Journal of Ophthalmology(English edition) 2019年第1期12卷 30-37页

作者： Rong-Rong Wang Cheng-Fang Li De-Zu Wang Cheng-Wu Zhang Gui-Xiang Liu Qingdao University Department of Ophthalmology Jimo Traditional Chinese Medicine Hospital Department of Ophthalmology Affiliated Qingdao Hiser Hospital of Qingdao University Institute of Advanced Materials Nanjing Tech University Department of Ophthalmology Affiliated Hospital of the Medical College of Qingdao University

AIM: To illustrate the isoform-specific role and mechanism of c-Jun n-terminal kinases(JnKs) in mouse optic nerve axotomy induced neurotrauma. METHODS: We firstly investigated the expression of JnK1, JnK2, and JnK3 in the retinal ganglion cells(RGCs) by double-immunofluorescent staining. Then we created optic nerve axotomy model in wild type as well as JnK1, JnK2, JnK3, isoform specific gene deficiency mice. With that, we checked the protein expression profile of JnKs and its active form, and quantified the survival RGCs number by immunofluorescence staining. We further explored the molecules underlying isoform specific protective effect by real-time polymerase chain reaction(PCR) and Western blotting assay. RESULTS: We found that all the three isoforms of JnKs were expressed in the RGCs. Deficiency of JnK3, but not JnK1 or JnK2, significantly alleviated optic nerve axotomyinduced RGCs apoptosis. We further established that expression of noxa, a pro-apoptotic member of BH3 family, was significantly suppressed only in JnK3 gene deficiency mice. But tumor necrosis factor receptor 1(TnFR1) and Fas, two key modulators of death receptor mediated apoptosis pathway, did not display obvious change in the expression. COnCLUSIOn: It is suggested that mitochondria mediated apoptosis, but not death receptor mediated apoptosis got involved in the JnK3 gene deficiency induced RGCs protection. Our study provides a novel insight into the isoform-specific role of JnKs in neurotrauma and indicates some cues for its therapeutics.

关键词： retinal ganglion cells c-Jun n-terminal kinases optic nerve mitochondria noxa neurotrauma

The n-terminal domain of nifA determines the temperature sensitivity of nif A in Klebsiella pneumoniae and Enterobacter cloacae

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Science China(Life Sciences) 2000年第1期43卷 8-15页

作者：顾剑颖俞冠翘朱家璧沈善炯 Laboratory of Molecular Genetics Shanghai Institute of Plant Physiology Chinese Academy of Sciences Shanghai 200032 China

The nifA protein is the central regulator of the nitrogen fixation genes. It activates transcription of nif genes by an alternative holoenzyme form of RnA polymerase containing the σ54 factor. The nifA protein from Klebsiella pneumoniae consists of the n-terminal domain of unknown function, the central catalytic domain with ATPase activity and the C-terminal DnA-binding domain. The Kp nifA protein is sensitive to temperature, while the Enterobacter cloacae nifA protein is less sensitive to temperature than Kp nifA. Our results show that the n-terminal domain of nifA plays the decisive role in the temperature sensitivity of the protein.

关键词： nifA protein temperature sensitivity n-terminal domain Enterobacter cloacae.

c-Jun n-terminal kinase-mediated Rubicon expression enhances hepatocyte lipoapoptosis and promotes hepatocyte ballooning

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World Journal of Gastroenterology 2016年第28期22卷 6509-6519页

作者： Akiko Suzuki Keisuke Kakisaka Yuji Suzuki Ting Wang Yasuhiro Takikawa division of hepatology department of internal medicine iwate medical university Morioka 0208505 Japan

AIM: To clarify the relationship between autophagy and lipotoxicity-induced apoptosis, which is termed "lipoapoptosis," in non-alcoholic steatohepatitis. METHODS: Male C57BL/6J mice were fed a high-fat diet(HFD) for 12 wk, after which the liver histology and expression of proteins such as p62 or LC3 were evaluated. Alpha mouse liver 12(AML12) cells treated with palmitate(PA) were used as an in vitro model. RESULTS: LC3-Ⅱ, p62, and Run domain Beclin-1 interacting and cysteine-rich containing(Rubicon) proteins increased in both the HFD mice and in AML12 cells in response to PA treatment. Rubicon expression was decreased upon c-Jun n-terminal kinase(JnK) inhibition at both the m RnA and the protein level in AML12 cells. Rubicon knockdown in AML12 cells with PA decreased the protein levels of both LC3-Ⅱ and p62. Rubicon expression peaked at 4 h of PA treatment in AML12, and then decreased. Treatment with caspase-9 inhibitor ameliorated the decrease in Rubicon protein expression at 10 h of PA and resulted in enlarged AML12 cells under PA treatment. The enlargement of AML12 cells by PA with caspase-9 inhibition was canceled by Rubicon ***: The JnK-Rubicon axis enhanced lipoapoptosis, and caspase-9 inhibition and Rubicon had effects that were cytologically similar to hepatocyte ballooning. As ballooned hepatocytes secrete fibrogenic signals and thus might promote fibrosis in the liver, the inhibition of hepatocyte ballooning might provide antifibrosis in the nASH liver.

关键词： Ballooned hepatocyte Caspase 9 c-Jun n-terminal kinase Rubicon SP600125

Effect of atorvastatin on serum oxidative stress and n-terminal brain natriuretic peptide expression in rats

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Asian Pacific Journal of Tropical Medicine 2014年第5期7卷 398-401页

作者： Yan Xu Yu Yang Ying-Quan Luo Department of Geratology Second Xiangya HospitalCentral South University

Objective:To investigate the effect of atorvastatin on serum oxidative stress and n-terminal brain natriuretic peptide expression in ***:A total of 40 healthy male SD rats were randomly divided into the sham group(Croup A,n=10,saline 5 mL/d),ischemia-reperfusion group(Group B,n=10,saline S mL/d),atorvastatin group(Group C,n=*** 20 mg/kg·d),atorvastatin + n-amino-arginine group(Group D,n=10,atorvastatin 20 mg/kg·d + n-amino arginine 15 mg/kg).Myocardial ischemia-reperfusion rat model was eslablished after 3 days of gavage.n-amino arginine 15 mg/kg was given by tail vein injection 15 min before *** reperfusion,enzymology indicators such us creatine kinase(CK) and lactate dehydrogenase and the oxidative stress parameters such as nitric oxide(nO),malondialdehyde(MDA) and total superoxide dismutase(TSOD),and n-terminal pro-brain natriuretic peptide(nT-proBnP)expression was detected by ***:LDH and CK levels of group A were significantly lower than the outer three groups,and group B was the *** was significant difference between group B and group C(Pnd no significant difference between group B and group D(P>0.05).MDA levels in group B were significantly higher than the other three *** lowest was group A,followed by group C,the difference among groups was significantly(Pnd nO levels in group B was the lowest,the level in group A was the highest,followed by group C,the difference among groups was significant(PnT-proBnP level in group B was significantly higher than the other three groups,the lowest was group A,followed by group C,the difference among groups was significant(Pnclusions:Atorvastatin has a protective effect on the myocardial injury in the myocardial ischemia and reperfusion *** can increase nO synthesis and decrease MDA content,increase serum TSOD activity and the oxidative stress effect,meanwhile protect myocardial cells and reduce myocardial injur

关键词： Atorvastatin Myocardial ischemia-reperfusion Oxidative stress n-terminal pro-brain natriuretic peptide

Plasma n-terminal pro-brain natriuretic peptide levels in elderly patients with isolated diastolic dysfunction

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Journal of Geriatric Cardiology 2005年第4期2卷 211-215页

作者： Yixin SOnG Qing LIn Xiaomin SHI Yunyun QI Department of Geriatric Medicine First Hospital of Beijing University Beijing 100034 China Department of Geriatric Medicine First Hospital of Beijing University Beijing 100034 China Department of Geriatric Medicine First Hospital of Beijing University Beijing 100034 China Department of Geriatric Medicine First Hospital of Beijing University Beijing 100034 China

Objective To investigate plasma n-terminal pro-brain natriuretic peptide (nT-BnP) levels and to assess their clinical significance in elderly patients with isolated diastolic dysfunction. Methods Plasma nT-BnP level were measured by electrochemiluminescence immunoassay in 34 symptomatic patients (Group 1), 34 asymptomatic patients (Group 2) with isolated diastolic dysfunction, and in 16 elderly healthy subjects (control group, Group 3), serving controls. Colored Doppler echocardiography was performed to evaluate the patients' cardiac structures and functions. Results The plasma nT-BnP level in Group 1 was significantly higher than those in Group 2 and Group 3 and increased with the severity of heart failure. There was no significant difference of plasma nT-BnP levels between Group 2 and Group 3 (p>0.05). A nT-BnP value of 102.75 pg/mL showed a sensitivity of 88.2%, a specificity of 87.5%, and an accuracy of 88.1% for diagnosing diastolic dysfunction. Patients with restrictive filling pattern on echocardiography had higher nT-BnP levels than those of impaired relaxation pattern (1961.2±304.9 versus 460.1±92.7pg/mL, pnclusion The elevation of plasma nT-BnP level in elderly patients with isolated diastolic dysfunction correlates with the severity of their diastolic abnormalities. The level of plasma nT-BnP has an important clinical value in the diagnosis of elderly patients with isolated diastolic dysfunction.

关键词： elderly isolated diastolic dysfunction n-terminal pro-brain natriuretic peptide echocardiography

n-terminal pro-B-type natriuretic peptide levels after hospital discharge in patients with acute myocardial infarction for predicting long term outcome

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South China Journal of Cardiology 2019年第3期20卷 168-173页

作者： ZHEnG Bao-juan CHEn Jian-jia MA dun-liang ZHUO Sheng-qing TIAn Xiang-ting Department of Cardiology Guangdong Cardiovascu lar InstituteGuangdong Academy of Medical ScienceGuangdong Provincial People's HospitalGuangzhou 510100 De partment of Cardiology Lecong HospitalFoshan 528315 Department of Cardiology Guangdong General Hospital's Nanhai HospitalFoshan 528200China

Background n-terminal-pro-brain natriuretic peptide(nT-pro-BnP)is associated with worse outcome in patients with acute myocardial infarction(AMI). However,the role of short-term follow-up of nT-pro-BnP level remains unclear. Methods Three hundred and sixty-two patients diagnosed with AMI were retrospectively enrolled in this study from March 2014 to March 2017 in our center. Blood samples were obtained at initial admission and again within 1 month after hospital discharge. The univariate and multivariate cox regression analyses including significant covariables were performed on nT-pro-BnP level at admission,discharge,or change from admission to discharge to predict adverse cardiovascular events(MACE)as study endpoints. Results There were 211 cases in nT-pro-BnP decrease group,while 151 cases in nT-pro-BnP increase group. The median follow-up was 365 days(interquartile range[IQR],322-861 days). After adjusting the covariables in the multiple logistic regression analysis,follow-up nT-pro-BnP level was still a significant independent predictor for MACE(OR,1.395;95% CI,1.102-1.869,P=0.005). However,the initial nT-pro-BnP level or change of nT-pro-BnP level had no significant predictive value for MACE. Conclusions A short-term follow-up nT-pro-BnP level after hospital discharge is a powerful prognostic biomarker for MACE in patients with AMI.[S Chin J Cardiol 2019;20(3):168-173]

关键词： n-terminal pro-B-type natriuretic peptide major adverse cardiovascular events prognosis

Growth differentiation factor-15 combined with n-terminal prohormone of brain natriuretic peptide increase 1-year prognosis prediction value for patients with acute heart failure: a prospective cohort study

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Chinese Medical Journal 2019年第19期132卷 2278-2285页

作者： Ji Hao Iokfai Cheang Li Zhang Kai Wang Hui-Min Wang Qian-Yun Wu Yan-Li Zhou Fang Zhou Dong-Jie Xu Hai-Feng Zhang Wen-Ming Yao Xin-Li Li Department of Cardiology The First Affiliated Hospital of Nanjing Medical UniversityNanjingJiangsu 210029China Department of Cardiology The Second Affiliated Hospital of Xuzhou Medical CollegeXuzhouJiangsu 221002China

Background:Clinical assessment and treatment guidance for heart failure depends on a variety of *** objective of this study was to investigate the prognostic predictive value of growth differentiation factor-15(GDF-15)and n-terminal prohormone of brain natriuretic peptide(nT-proBnP)in assessing hospitalized patients with acute heart failure(AHF).Methods:In total,260 patients who were admitted for AHF in the First Affiliated Hospital of nanjing Medical University were enrolled from April 2012 to May *** history and blood samples were collected within 24 h after the *** primary endpoint was the all-cause mortality within 1 *** patients were divided into survival group and death group based on the *** established mortality risk factors and serum GDF-15 level,receiver-operator characteristic(ROC)analyses were *** regression analyses were used to further analyze the combination values of nT-proBnP and ***:Baseline GDF-15 and nT-proBnP were significantly higher amongst deceased than those in survivors(Pn ROC analyses,area under curve(AUC)for GDF-15 to predict 1-year mortality was 0.707(95%confidence interval[CI]:0.648–0.762,Pnd for nT-proBnP was 0.682(95%CI:0.622–0.738,Pno statistically significant difference was found between the two markers(P=0.650).Based on the optimal cut-offs(GDF-15:4526.0 ng/L;nT-proBnP:1978.0 ng/L),the combination of GDF-15 and nT-proBnP increased AUC for 1-year mortality prediction(AUC=0.743,95%CI:0.685–0.795,Pnclusions:GDF-15,as a prognostic marker in patients with AHF,is not inferior to *** the two markers could provide an early recognition of high-risk patients and improve the prediction values of AHF long-term *** trial registration:ChiCTR-OnC-12001944,http://***.

关键词： Growth differentiation factor-15 Heart failure n-terminal pro-B type natriuretic peptide Prognosis

Signal Transduction Pathways Mediated by Secreted and non-Secreted Forms of Intact Insulin-Like Growth Factor Binding Protein-3 (IGFBP-3) and Its 1-97 n-terminal Fragment in PC-3 Human Prostate Cancer Cells

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Journal of Cancer Therapy 2013年第8期4卷 1290-1297页

作者： Hanief Mohammad Shahjee Benjamin Kefas nisan Bhattacharyya Mohamed K. Radwan Department of Biochemistry and Biophysics University of Rochester Medical Center Rochester USA Diabetes Branch NIDDK National Institutes of Health Bethesda USA Division of neuro-Oncology Neurology Department University of Virginia Health System Charlottesville USA

Our previous results indicated that both the secreted and the intracellular form of full length and 1-97 n-terminal fragment of IGFBP-3 induce apoptosis in PC-3 human prostate cancer cells in an IGF-dependent and independent manner. This study was undertaken to delineate possible down-stream signaling pathways that are involved in this process. Intact IGFBP-3 and its n-terminal 1-97 fragments with or without a signal propeptide were fused to YFP and expressed in PC-3 human prostate cancer cells. In some cases, the putative IGF-binding site was presented in full length IGFBP-3 and its n-terminal fragment was also mutated. Extent of apoptosis was quantified using FACS. Up-regulation of total Stat-1 and activation of phospho-Stat-1 were shown by western blot. TGF-β signal was measured by luciferase reporter assay. Results from inhibitor studies indicated that both the Caspase 8 and caspase 9 pathways are involved in IGFBP-3 (non-secreted form) which induced apoptosis in PC-3 cells. Exogenous addition of IGFBP-3 to PC-3 cells increased Stat-1 protein expression/tyrosine phosphorylation. Interestingly, results also showed that knockdown of Stat-1 by siRnA potentiated the IGFBP-3 induced apoptosis in PC-3 cells. In addition, both full-length IGFBP-3 and its 1-97 nterminal fragments inhibited TGF-β signaling in these cells. This is the first report that compares the signal transduction pathways involved in apoptotic pathways mediated by IGFBP-3 in PC-3 human prostate cancer cells. non-secreted form of full length IGFBP-3 and its n-terminal fragments induced apoptosis in PC-3 cells via activation of caspase 8 and caspase 9. Although, only non-secreted form of IGFBP-3 is involved in inducing apoptosis in PC-3 cells via caspase 8 and caspase 9 activation pathways but both secreted and non-secreted forms of IGFBP-3 are involved in modulating Stat-1 and TGF-β pathways to induce apoptotic actions in PC-3 cells. non-secreted intact IGFBP-3 and its n-terminal fragments induced apo

关键词： n-terminal Fragment Apoptosis Caspases Human Prostate Cancer Cells