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Determination of degree of substitution for n-acylated chitosan using IR spectra

Science China Chemistry 2001年第2期44卷 216-224页

作者：董炎明许聪义汪剑炜王勉吴玉松阮永红 Department of Materials Science and Engineering State Key Laboratory for Physical Chemistry of Solid Surfaces Xiamen University Xiamen China

Chitosans with various degrees of deacetylation (D.D.), which were used as standard sample for FTIR determination, were prepared from completely deacetylated chitosan by homogeneous n-acetylation reaction. By combining four probable probe bands, i.e. 1655, 1560, 1380 and 1320 cm-1, eight probable reference bands, i.e. 3430, 2920, 2880, 1425, 1155, 1070, 1030 and 895 cm-1 and two baseline methods, the most suitable ratios Aprobe band/Areference band from IR spectra to determine the degree of acetylation of chitosan were evaluated from 48 combinations to be A1560/A2880, A1560/A2920 and A1655/A3430(A1560/A2880 is mostly recommended). The second baseline method, i.e. linking between adjacent two valleys, was better for measuring the absorbances of 1560 and 1655 cm-1 bands. The determination range of the D.D. (1%-100%) covered almost the whole range. The standard curves with A1560/A2880 and A1655/A3430 were also suitable for the determination of degree of substitution of other n-acylated chitosan, such as n-propionyl chitosan, n-butyryl chitosan and n-hexanoyl chitosan.

关键词： chitin, chitosan, n-acylated chitosan, IR spectra, degree of deacetylation, degree of substitution

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new Amphiphilic Amino Acid Derivatives for Efficient DnA Transfection in Vitro

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Advances in Chemical Engineering and Science 2017年第2期7卷 191-205页

作者： Lucía C.Pena María F.Argaraná María M.De Zan Antonella Giorello Sebastián Antuna Claudio C.Prieto Carolina M.I.Veaute Diana M.Müller LAQUIMAP Dto.Química OrgánicaUniversidad Nacional del LitoralSanta FeArgentina Cát.Microbiología General Universidad Nacional del LitoralSanta FeArgentina Laoratorio de Control de Medicamentos Universidad Nacional del LitoralSanta FeArgentina Instituto de Investigaciones en Catálisis y Petroquímica Universidad Nacional del LitoralSanta FeArgentina Laboratorio de Cultivos Celulares Universidad Nacional del LitoralSanta FeArgentina Laboratorio de Inmunología Básica Universidad Nacional del LitoralSanta FeArgentina

nucleic acids-based therapies have recently developed as next-generation agents for treating and preventing viral infection, cancer, and genetic disorders, but their use is still limited due to its relatively poor delivery into targeted cells. We designed and synthesized new amphiphilic amino acid derivatives (cysteine-based) of low molecular weight, formed by the same pentapeptide (AG2: WWCOO) n-acylated, with different hydrophobic chains containing from 12 to 18 carbons, named AG2-Cn (n), which dimerize by oxidation in the presence of pLenti-CMV-GFP Puro plasmid (P) in the respective gemini. We determined transfection efficiency, critical micelle concentration, particle size, ζ-potential and cytotoxicity for the derivatives obtained. We found that all the synthesized compounds were active for DnA delivery and had greater ability to transfect CHO-K1 cells. In particular, AG2-C18 is a promising carrier for gene delivery because it showed no cytotoxicity and its activity was greater than or equal to the commercial actives currently used.

关键词： Amphiphile n-acylated Cysteine Gemini Ornithine Transfection

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