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Occurrence of n-acyl Homoserine Lactones in Extracts of Bacterial Strain of Pseudomonas aeruginosaand in Sputum Sample Evaluated by Gas Chromatography–Mass Spectrometry

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American Journal of Analytical Chemistry 2011年第2期2卷 294-302页

作者： Susheela Rani Ashwini Kumar Ashok Kumar Malik P. Schmitt-Kopplin 不详

This study presents a fast, accurate and sensitive technique using gas chromatography-mass spectrometry (GC-MS) for the identification and quantification of n-acyl homoserine lactones (AHLs) in the extracts of bacterial strain of Pseudomonas aeruginosa and sputum sample of a cystic fibrosis patient. This method involves direct separation and determination of AHLs by using GC-MS as simultaneous separation and characterization of AHLs were possible without any prior derivatiza-tion. Electron ionization resulted in a common fragmentation pattern with the most common fragment ion at m/z 143 and other minor peaks at 73, 57 and 43. The limit of detection for n-butanoyl, n-hexanoyl, n-octanoyl, n-decanoyl, n-dodecanoyl and n-tetradecanoyl homoserine lactones was 2.14, 3.59, 2.71, 2.10, 2.45 and 2.34 μg/L, respectively. The presence of AHLs in the culture of P. aeruginosa strain and spu-tum of a cystic fibrosis patient was achieved in selected ion monitoring (SIM) mode by using the prominent fragment at m/z 143.

关键词： Gas Chromatography—Mass Spectrometry n-acyl Homoserine Lactone (n-Butanoyl n-Hexanoyl n-Octanoyl n-Decanoyl n-Dodecanoyl and n-Tetradecanoyl) Homoserine Lactone Sputum Sample Bacterial Strain

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n-acylhomoserine Lactones (AHLs), QseB/C Gene Detection, Virulence Factors and Antibiotics Resistance of Aeromonas hydrophila

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Advances in Microbiology 2019年第5期9卷 495-506页

作者： Emmanuel Konadu Sarkodie Shuxin Zhou Weihua Chu Department of Pharmaceutical Microbiology School of Life Science and Technology China Pharmaceutical University Nanjing China

The aim of this research was to detect the n-acyl homoserine lactones (AHLs) production and QseB/C gene of Aeromonas hydrophila. We analyzed the potentials of these isolates of Aeromonas hydrophila in causing biofilm formation, hemolysis, protease, and lipase. The antibiotic susceptibility of the 15 Aeromonas hydrophila isolates was also investigated. The detection of AHLs was carried out using the Chromobacterium violaceum strain CV026 as biosensors. The isolated strains were tested for the reaction of C. violaceum CV026 by cross-streaking on an agar plate. Production of AHLs was determined by the diffusing via the agar plates and the tinge of the biosensor strains. All isolated strains produced AHLs. A polymerase chain reaction (PCR) showed the isolated strains had qseB and qseC genes. Susceptibility tests of A. hydrophila isolates were administered against 25 different antibiotic disks representing 12 classes of antibiotics. The strains were highly resistant to β-Lactam with 96.7% showing resistibility, whereas 97.7% susceptibility was found towards Aminoglycoside class of the antibiotic used. 60% showed intermediate resistant to Polypeptide. 100% of the strains showed no resistant to Aminoglycoside, Polypeptide, Monobactam, and Carbapenems class of antibiotics. Each of the isolates was found to be associated with at least one virulent factor. Our results clearly demonstrated that there is a presence of QseB/C genes in A. hydrophila and also produces AHLs molecule and virulence factors. The investigated isolates showed the pathogenic potential of Aeromonas hydrophila which makes it a serious threat to public health.

关键词： Aeromonas hydrophila Antibiotic Susceptibility Virulence Factors Biofilm Formation n-acyl Homoserine Lactones (AHLs)

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