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Interaction of the Bioherbicide myrothecium verrucariawith Technical-Grade Glyphosate on Glyphosate-Susceptible and -Resistant Palmer Amaranth

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American Journal of Plant Sciences 2018年第11期9卷 2306-2319页

作者： Robert E. Hoagland C. Douglas Boyette Robin H. Jordan Kenneth C. Stetina Crop Production Systems Research Unit USDA-ARS Stoneville MS USA Biological Control of Pests Research Unit USDA-ARS Stoneville MS USA

Previously we found that a strain of myrothecium verrucaria (MV) exhibited bioherbicidal activity against several important weeds, and that some commercial formulations of glyphosate applied with MV resulted in synergistic interactions that improved weed control efficacy. We also found that MV had bioherbicidal activity against glyphosate-resistant Palmer amaranth. We have also reported that some commercial formulations are inhibitory to MV. Our objectives were to test the effect of unformulated glyphosate (high purity, technical-grade glyphosate) alone and in combination with MV for bioherbicidal activity on glyphosate-susceptible and -resistant Palmer amaranth biotypes under greenhouse conditions and to examine technical-grade glyphosate on the growth of this bioherbicide. High purity glyphosate (without adjuvants/surfactants) was not toxic to MV growth and sporulation at concentrations up to 2.0 mM when grown on agar supplemented with the herbicide. Both biotypes were injured by MV and MV plus glyphosate treatments as early as 19 h after application (3 h after a dew period of 16 h). These injury effects increased and were more evident through the 6-day time course, when after 120 h the MV plus glyphosate treatment had killed all glyphosate-susceptible and -resistant plants. The interaction of glyphosate plus MV was synergistic toward the control of Palmer amaranth. Data strongly suggest that the active ingredient is responsible for the synergy previously found when this bioherbicide was combined with some commercial formulations of glyphosate. Results demonstrated that MV can control both glyphosate-resistant and -susceptible Palmer amaranth seedlings and act synergistically with high-purity glyphosate to provide improved weed control.

关键词： Amaranthus palmeri Biocontrol Agent Bioherbicide Biological Weed Control Glyphosate-Resistance myrothecium verrucaria Palmer Amaranth Pigweed

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Cucurbit Host Range of myrothecium roridumIsolated from Watermelon

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American Journal of Plant Sciences 2012年第3期3卷 353-359页

作者： Wayne W. Fish Benny D. Bruton Thomas W. Popham USDA-ARS Stillwater USA Wes Watkins Agricultural Research Laboratory USDA-ARS Lane USA

In 2010, a foliar and stem-lesion disease that produced moderate to severe defoliation of watermelon was observed in the southern Great Plains. The disease was ultimately determined to be caused by myrothecium roridum. The objective of this study was to compare the susceptibility of the vegetation and fruit of a broad range of commercially important cucurbits to three isolates obtained from these foliar lesions on watermelon. In greenhouse foliar inoculation experiments, cantaloupe, honeydew, cucumber, squash, and watermelon were susceptible to the fungus with cantaloupe and honeydew being the most susceptible and watermelon the most resistant. Furthermore, greenhouse inoculations supported earlier field observations as differential resistance was exhibited among the watermelon cultivars as well as the cucurbit types. All tested cucurbit fruit exhibited interior lesions when inoculated sub-epidermally with M. roridum isolates. However, natural infection of watermelon and pumpkin fruit has never been reported.

关键词： myrothecium roridum Watermelon Leaf and Stem Blight Cucurbits

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Interaction of a myrothecium verrucariaMycelial Preparation and a Glyphosate Product for Controlling Redvine (Brunnichia ovata) and Trumpet Creeper (Campsis radicans)

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American Journal of Plant Sciences 2020年第2期11卷 201-213页

作者： Clyde Douglas Boyette Robert E.Hoagland Charles T.Bryson Mark A.Weaver Kenneth C.Stetina USDA-ARS Biological Control of Pests Research UnitStonevilleMSUSA USDA-ARS Crop Production and Systems Research UnitStonevilleMSUSA

A mycelial formulation of the bioherbicidal fungus myrothecium verrucaria (Alb. & Schwein.) Ditmar: Fr. (MV) was tested alone and in combination with a commercially available glyphosate [N-(phosphonomethyl)glycine] (GLY) product for controlling the invasive vines, redvine [Brunnichia ovata (Walt.) Shinners], and trumpet creeper [Campsis radicans (L.) Seem. ex Bureau] in field experiments conducted near Stoneville, MS. Several application timing regimens were evaluated (Fall, Spring, Fall + Spring, and Spring + Fall). We found that a Fall + Spring application of MV + GLY controlled redvine and trumpet creeper by 95%, 12 days after the second treatment, through a synergistic interaction of the fungus and glyphosate. Disease symptomatology was characterized by rapid necrosis of leaf and stem tissues, with mortality occurring within 72 h. Neither glyphosate alone, nor MV alone, effectively controlled either weed species under any application timing regimen. No visual disease or herbicide damage occurred on glyphosate-resistant soybean plants in the treated test plots. These results suggest that some formulations of glyphosate, mixed with the bioherbicide MV, can effectively control redvine and trumpet creeper, two of the most troublesome weeds in the row crops of the Mississippi Delta region in the mid-southern U.S.

关键词： Bioherbicide Interaction Mycelial Formulation myrothecium verrucaria Campsis radicans Brunnichia ovata

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Extending the Shelf-Life of myrothecium verrucaria, a Bioherbicide

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American Journal of Plant Sciences 2017年第12期8卷 3272-3484页

作者： Robert E. Hoagland C. D. Boyette K. C. Stetina United States Department of Agriculture-Agricultural Research Service (USDA-ARS) Crop Production Systems Research Unit Stoneville MS USA United States Department of Agriculture-Agricultural Research Service (USDA-ARS) Biological Control of Pests Research Unit Stoneville MS USA

The shelf-life of a bioherbicide product is an important factor with regard to its commercial potential. The bioherbicidal efficacy of freshly fermented myrothecium verrucaria (strain IMI 368023) (MV) mycelia formulations and MV mycelia preparations that had been freeze-dried and then stored at -20°C for 8 years was compared. Two concentrations of each formulation (1.0x and 0.5x) were tested, utilizing bioassays on seedlings of the weed, hemp sesbania (Sesbania exaltata) under greenhouse conditions or in darkness utilizing hydroponically grown seedlings. Freeze drying of freshly prepared MV mycelium produced a light, brownish-colored powder. Efficacy tests of this reconstituted 8-year-old dried material showed that some bioherbicidal activity was lost during long-term storage, i.e., ~20% and ~60% seedling dry weight reduction at the 1.0x and 0.5x rate, respectively. Although plant mortality was greater in the fresh mycelial preparations treatments versus the freeze-dried and stored samples at all time points in the time-course, the stored material still caused >80% mortality, 15 days after treatment. Comparative disease progression ratings also showed a similar trend. Overall results show that freeze-drying MV is a useful method to reduce the bulk and cumbersomeness of storing heavy liquid fermentation product, while retaining bioherbicidal activity. These findings increase the utility of this bioherbicide and offer the potential to use the dried material in soil treatments or in a more concentrated form than attainable via the fermented product.

关键词： Bioherbicide Plant Pathogen Weed Control Lyophilization Freeze-Drying Mycelium Mycoherbicide myrothecium verrucaria

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Effects of myrothecium verrucariaon Ultrastructural Integrity of Kudzu (Pueraria montana var. lobata) and Phytotoxin Implications

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American Journal of Plant Sciences 2012年第11期3卷 1513-1519页

作者： Robert E. Hoagland C. Douglas Boyette Kevin C. Vaughn Neal D. Teaster Ken Stetina United States Department of Agriculture-Agricultural Research Service (USDA-ARS) Crop Production Systems Research Unit Stoneville USA USDA-ARS Biological Control of Pests Research Unit Stoneville USA.

The fungus myrothecium verrucaria (Alb. & Schwein.) (MV), originally isolated from diseased sicklepod (Senna obtusifolia L.), has bioherbicial activity against kudzu and several other weeds when applied with low concentrations of the surfactant Silwet L-77. To more fully understand the initial events of MV infection or disease progression, and to improve knowledge related to its mechanism of action, the effects of MV and its product (roridin A) on kudzu seedlings were examined at the ultrastructural level. Ultrastructural analysis of MV effects on kudzu seedlings revealed a rapid (~1 h after treatment) detachment of the protoplast from the cell wall and plasmodesmata appeared to be broken off and retained in the wall. These symptoms occurred well in advance of the appearance of any fungal growth structures. Some fungal growth was observed after severe tissue degeneration (24 to 48 h after treatment), but this occurred primarily at the extra-cellular location with respect to the kudzu tissues. Kudzu seedlings treated with roridin A, a trichothecene produced by the fungus, exhibited some symptoms similar to those induced by the fungus applied in spore formulations with surfactant. The overall results are the first to report the ultrastructural effects of this bioherbicide on plants and suggest that penetration of a phytotoxic substance(s) in the fungal formulation was facilitated by the surfactant, and that roridin A exerts phytotoxicity toward kudzu.

关键词： Bioherbicide Biological Weed Control Kudzu myrothecium verrucaria Ultrastructure Trichothecene

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黄花蒿内生菌myrothecium roridum IFB-E091产生的一个新吲哚衍生物

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药学学报 2015年第10期50卷 1305-1308页

作者：申丽李玲玉张小君李明宋勇春扬州大学医学院江苏扬州225001 南京大学功能生物分子研究所江苏南京210046

从黄花蒿内生菌myrothecium roridum IFB-E091固体发酵产物中分离获得3个化合物,分别被鉴定为:(S)-(-)-N-[2-(3-Hydroxy-2-oxo-2,3-dihydro-1H-indol-3-yl)-ethyl]-acetamide(1)、N-(4-hydroxyphenethyl)acetamide(2)和asperfumoid(3),其中化合物1为新吲哚衍生物。体外细胞毒活性测定表明,化合物1对人肝癌细胞SMMC-7721和人宫颈癌细胞He La没有明显的抑制活性。

关键词：植物内生菌 myrothecium roridum 次生代谢产物结构鉴定

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Interaction of the Bioherbicide myrothecium verrucaria and Glyphosate for Kudzu Control

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American Journal of Plant Sciences 2014年第26期5卷 3943-3956页

作者： Clyde Douglas Boyette Robert E. Hoagland Mark A. Weaver Kenneth C. Stetina United States Department of Agriculture-Agricultural Research Service (USDA-ARS) Biological Control of Pests Research Unit Stoneville MS USA United States Department of Agriculture-Agricultural Research Service (USDA-ARS) Crop Production Systems Research Unit Stoneville MS USA

Kudzu is an exotic invasive weed in the southeastern U.S. that is difficult to control with current commercial herbicides. Some success for its control has been achieved using a bioherbicidal agent, myrothecium verrucaria (MV). Spore and mycelial formulations of MV were tested alone and in combination with glyphosate for control of kudzu (Pueraria lobata) under greenhouse and field conditions in naturally-infested areas. In greenhouse and field experiments, kudzu control increased as the concentration of spores or mycelia increased. Glyphosate alone provided 10%, 35%, 50% and 60% control in field experiments at 0.25, 0.50, 0.75 and 1.0X rates, respectively and MV alone spores provided 15%, 50%, 65% and 85% control at 0.25, 0.50, 0.75 and 1.0X rates, respectively. However, when MV spores were combined with glyphosate, significantly higher control occurred than that caused by either component alone. Similar levels of control were observed for MV mycelial formulations applied alone or with glyphosate at equivalent concentrations of the fungus. The rate of disease progression was more rapid and severe at all fungal spore or mycelial formulations and herbicide rates when these propagules were applied in combination with glyphosate. In field tests, 24 h after application, only 20% of kudzu plants were severely damaged by MV alone (0.25X), whereas 80% were severely diseased when MV spores and glyphosate were mixed and applied at 0.25X rates each. A similar trend occurred with the MV mycelial formulation applied at these rates. Synergist interactions on kudzu control were observed, especially when lower levels of MV (spores or mycelia) and glyphosate were combined and applied to kudzu in the greenhouse or in the field. These results suggest that it may be possible to incorporate glyphosate to improve the bioherbicidal control potential and reduce herbicide and inoculum requirements of M. verrucaria spores or mycelium for controlling kudzu.

关键词： Bioherbicide Glyphosate Kudzu myrothecium verrucaria Pueraria lobata

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