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Effect and mechanism of Irbesartan on occurrence of ventricular arrhythmias in rats with myocardial ischemia through connexin43(cx43)

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Asian Pacific Journal of Tropical Medicine 2016年第10期9卷 985-990页

作者： Tao Wu Dan Wu Qinghua Wu Bing Zou Xiao Huang Xiaoshu Cheng Yanqing Wu Kui Hong Ping Li Department of Cardiovascular Medicine the Second Affiliated Hospital of Nanchang University Jiangxi Health Vocational College

Objective: To explore the effect and mechanism of angiotensin II receptor blockers-Irbesartan on occurrence of ventricular arrhythmias in rats with myocardial ischemia. Methods: Rats with embryonic cardiomyocytes-H9c2 were randomly divided into control group. ischemia group. Irbesartan group and Irbesartan+ischemia group. The cell viability of rats in each group was tested using MTT. Real-time PCR was employed to detect the expression of connexin43 (Cx43) mRNA and western blot to detect the expression of Cx43 and phosphorylated Cx43. SD rats were randomly divided into the sham-operation group (SO). myocardial infarction group (MI). Irbesartan group and MI+ Irbesartan group, with 10 rats in each group. HE staining was employed to observe the change in the pathomorpholouy of left ventricular tissue and TUNEL method to analyze the cell apoptosis in the tissue. The immunofluorescence was adopted to observe the expression and distribution of Cx43 in the left ventricular myocardium and study the change in the expression of Cx43 in the cardiac muscular tissue at mRNA and protein level. Results: The intervention of lrbesartan in the condition of ischemia indicated the significant decrease in the number of necrotic cells. The expression of Cx43 was significantly decreased under the culture of ischemia (P<0.05), but in the presence of Irbesartan, the expression of Cx43 was increased compared with the ischemia group (p<0.01). The results of WB assay showed the similar trend of change at mRNA level. There was the significant difference in the score of ventricular arerythmia between MI group and SO group (P<0.01). The incidence of ventricular tachycardia or ventricular fibrillation was significantly increased compared with the one in SO group (P<0.05). There was the significant difference in the overall score between MI+Irbesartan group and MI group (P<0.05). The expression of Cx43 in the cardiac muscular tissue in MI group was significantly decreased (P<0.01(US) SO group). But

关键词： Irbesartan myocardial lschemia Cx43 Connexin Arrhythmias

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Effects of Acupuncture at Acupoint Neiguan(PC6)and Intravenous Injection of Larginine on the Improvement of Acute myocardial Ischemia

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Journal of Acupuncture and Tuina Science 2006年第3期4卷 136-139页

作者：周培华吕娟秀朱大年朱忠春(译) Teaching and Research Section of Physiology Shanghai Medical Workers College Shanghai 200037 ER. China Ningxia Medical College Yinchuan 750004 P.R. China Department of Physiology & Pathophysiology Shanghai Medical college of Fudan University Shanghai 200032 P.R. China 不详

Objective： The present study is to observe and compare the different effects of electroacupuncture（EA） at acupoint Neiguan （PC 6）, intravenous administration of L-arginine（L-Arg, a precursor of nitric oxide）, and the drug administration combined with the EA on acute myocardial ischemia（AMI） in rats. Methods. The rat model of AMI was established by occlusion of the anterior descending branch of the left coronary artery, then the animals were divided randomly into 5 groups, i.e. control group, AMI group, L-Arg treatment group, EA treatment group and L-Arg plus EA treatment group, the mean arterial pressure（MAP）, heart rate（HR） and the cardiac functions were observed and recorded. Results： The data showed that MAP, HR and all the indices of cardiac functions in the AMI group were significantly lower than those in control group, and the indices in each treatment group were all improved to different extents, especially in L-Arg plus EA treatment group. *** combined use of L-Arg and EA had the better therapeutic effect on the AMI.

关键词： Larginine Nitric oxide Electroacupuncture myocardial lschemia

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Effect of Acupuncture at Acupoint Neiguan(PC6)on the myocardial c-fos Gene Expression of Acute myocardial Ischemia in Rats

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Journal of Acupuncture and Tuina Science 2006年第3期4卷 132-135页

作者：谢芳梁勋厂吴红金茹立强朱忠春(译) Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 P. R. China Hospital of the Integrated Chinese and Western Medicine Affiliated to Beijing university of TCM Beijing 1000130 P. R. China 不详

Objective：To investigate the mechanism of improvement effect of electro acupuncture at acupoint Neiguan （PC 6） on acute myocardial ischemia （AMI）. Methods： Ninety-six rats were randomly divided into sham-operation group, myocardial ischemia model group and myocardial ischemia model plus electro-acupuncture group, the rat model of AMI was established by occlusion of the anterior descending branch of the left coronary artery, then electro-acupuncture therapy was performed at bilateral acupoint Neiguan （PC 6）. The myocardial enzyme activities in plasma was detected with biochemical method, and the myocardial c-fos gent expression was detected with reverse transcription polymerase chain reaction（RT PCR） method. Results： The myocardial enzyme activities in plasma and the myocardial c-fos gene expression in myocardial ischemia model group were obviously higher than those in sham group（P〈 0.05）, after electro-acupuncture treatment, the myocardial enzyme activities in plasma and the myocardial c-fos gene expression were decreased, there was a significant difference （P〈 0.05）. Conclusion. The mechanism of improvement effect of electro acupuncture at acupoint Neiguan （PC 6） on AMI is related to down-regulation of the myocardial c-fos mRNA expression.

关键词： Electro-acupuncture Point, PC 6 myocardial lschemia Gene Expression

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