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A circular single-stranded DNA mycovirus infects plants and confers broad-spectrum fungal resistance

Molecular Plant 2024年第6期17卷 955-971页

作者： Xianhong Wang Ioly Kotta-Loizou Robert H.A.Coutts Huifang Deng Zhenhao Han Ni Hong Karim Shafik Liping Wang Yashuang Guo Mengmeng Yang Wenxing Xu Guoping Wang National Key Laboratory for Germplasm Innovation&Utilization of Horticultural Crops Wuhan 430070China Hubei Hongshan Laboratory Wuhan 430070China College of Plant Science and Technology Huazhong Agricultural UniversityWuhan 430070China Key Lab of Plant Pathology of Hubei Province Wuhan 430070China Department of Life Sciences Faculty of Natural SciencesImperial College LondonLondon SW72AZUK Department of Clinical Pharmaceutical and Biological ScienceSchool of Life and Medical SciencesUniversity of HertfordshireHatfield AL109ABUK Department of Plant Pathology Faculty of AgricultureAlexandria UniversityAlexandria 21526Egypt

Circular single-stranded DNA(ssDNA)viruses have been rarely found in fungi,and the evolutionary and ecological relationships among ssDNA viruses infecting fungi and other organisms remain *** this study,a novel circular ssDNA virus,tentatively named Diaporthe sojae circular DNA virus 1(DsCDV1),was identified in the phytopathogenic fungus Diaporthe sojae isolated from pear ***1 has a monopartite genome(3185 nt in size)encapsidated in isometric virions(21-26 nm in diameter).The genome comprises seven putative open reading frames encoding a discrete replicase(Rep)split by an intergenic region,a putative capsid protein(CP),several proteins of unknown function(P1-P4),and a long intergenic ***,the two split parts of DsCDV1 Rep share high identities with the Reps of Geminiviridae and Genomoviridae,respectively,indicating an evolutionary linkage with both *** analysis based on Rep or CP sequences placed DsCDV1 in a unique cluster,supporting the establishment of a new family,tentatively named Gegemycoviridae,intermediate to both ***1 significantly attenuates fungal growth and nearly erases fungal virulence when transfected into the host ***,DsCDV1 can systematically infect tobacco and pear seedlings,providing broad-spectrum resistance to fungal *** localization analysis revealed that DsCDV1 P3 is systematically localized in the plasmodesmata,while its expression in trans-complementation experiments could restore systematic infection of a movement-deficient plant virus,suggesting that P3 is a movement ***1 exhibits unique molecular and biological traits not observed in other ssDNA viruses,serving as a link between fungal and plant ssDNA viruses and presenting an evolutionary connection between ssDNA viruses and *** findings contribute to expanding our understanding of ssDNA virus diversity and evolution,offering potential biocontrol applications for managing crucial plant d

关键词： circular single-stranded DNA virus mycovirus virus evolution Genomoviridae Gegemycoviridae Diaporthe sojae circular DNA virus 1

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A 2-kb mycovirus Converts a Pathogenic Fungus into a Beneficial Endophyte for Brassica Protection and Yield Enhancement

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Molecular Plant 2020年第10期13卷 1420-1433页

作者： Hongxiang Zhang Jiatao Xie Yanping Fu Jiasen Cheng Zheng Qu Zhenzhen Zhao Shufen Cheng Tao Chen Bo Li Qianqian Wang Xinqiang Liu Binnian Tian David B.Collinge Daohong Jiang State Key Laboratory of Agricultural Microbiology College of Plant Science and TechnologyHuazhong Agricultural UniversityWuhanHubei Province 430070China The Provincial Key Lab of Plant Pathology of Hubei Province Huazhong Agricultural UniversityWuhanChina Department of Plant and Environmental Sciences University of Copenhagen1871 Frederiksberg CDenmark

mycoviruses are viruses that infect fungi,and hypovirulence-associated mycoviruses have the potential to control fungal ***,it is unclear how mycovirus-mediated hypovirulent strains live and survive in the field,and no mycovirus has been applied for field crop *** this study,we found that a previously identified small DNA mycovirus(SsHADV-1)can convert its host,Sclerotinia sclerotiorum,from a typical necrotrophic pathogen to a beneficial endophytic ***-1 downregulates the expression of key pathogenicity factor genes in *** during *** growing in rapeseed,the SsHADV-1-infected strain DT-8 significantly regulates the expression of rapeseed genes involved in defense,hormone signaling,and circadian rhythm *** a result,plant growth is promoted and disease resistance is *** experiments showed that spraying DT-8 at the early flowering stage can reduce the disease severity of rapeseed stem rot by 67.6%and improve yield by 14.9%.Moreover,we discovered that SsHADV-1 could also infect other *** strains on DT-8-inoculated plants and that DT-8 could be recovered from dead *** findings suggest that the mycoviruses may have the ability to shape the origin of *** discoveries suggest that mycoviruses may influence the origin of endophytism and may also offer a novel strategy for disease control in which mycovirus-infected strains are used to improve crop health and release mycoviruses into the field.

关键词： mycovirus Sclerotinia sclerotiorum endophyte rapeseed stem rot biological control Brassica napus

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A novel ambigrammatic mycovirus,PsV5,works hand in glove with wheat stripe rust fungus to facilitate infection

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Plant Communications 2023年第3期4卷 112-125页

作者： Yanhui Zhang Xiaofei Liang Mengxin Zhao Tuo Qi Hualong Guo Jing Zhao Jie Zhao Gangming Zhan Zhensheng Kang Li Zheng State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection Northwest A&F UniversityYanglingShaanxi 712100China Sanya Nanfan Research Institute of Hainan University Hainan Yazhou Bay Seed LaboratorySanya 572025China Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests Ministry of Education and School of Plant ProtectionHainan UniversityHaikouHainan 570228China State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China State Key Laboratory of Hybrid RiceKey Laboratory of Major Crop Diseases&Collaborative Innovation Center for Hybrid Rice in Yangtze River BasinRice Research InstituteSichuan Agricultural University at WenjiangChengduSichuan 611130China

Here we describe a novel narnavirus,Puccinia striiformis virus 5(PsV5),from the devastating wheat stripe rust fungus *** ***(Pst).The genome of PsV5 contains two predicted open reading frames(ORFs)that largely overlap on reverse strands:an RNA-dependent RNA polymerase(RdRp)and a reverseframe ORF(rORF)with unknown *** translations of both ORFs were demonstrated by immune *** wheat lines overexpressing PsV5(RdRp-rORF),RdRp ORF,or rORF were more susceptible to Pst infection,whereas PsV5-RNA interference(RNAi)lines were more *** of PsV5(RdRp-rORF),RdRp ORF,or rORF in Fusarium graminearum also boosted fungal *** thus report a novel ambigrammatic mycovirus that promotes the virulence of its fungal *** results are a significant addition to our understanding of virosphere diversity and offer insights for sustainable wheat rust disease control.

关键词： Puccinia striiformis f.sp.tritici mycovirus ambigrammatic virulence-enhancing

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Complete genomic sequenceand organization of a novel mycovirus from Phoma matteuciicola strain LG915

Complete genomic sequenceand organization of a novel mycovir...

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中国植物病理学会2019年学术年会

作者： Zheng Fan Xu Gang Zhou Jia Xie Changping Cui Hongguang Miao Weiguo Kang Zhensheng Zheng Li College of Plant Protection Hainan University/Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and PestsMinistry of Education State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection Northwest A&F University

The complete genome of a double-stranded RNA(dsRNA) mycovirus,Phoma matteuciicola partitivirus 1(PmPV1) was *** consists of two dsRNA segments with 1 664 bp(dsRNA-1) and 1 383 bp(dsRNA-2) in length and each containing a single open reading frame(ORF)and potentially encoded 46.78 ku and 40.92 ku proteins,***-1 encodes a putative polypeptide with a conserved RNA—dependent RNA polymerase(RdRp) domain and showed similarity to that of *** protein encoded by dsRNA-2 has no significant similarity to the typical coat protein(CP) of the partitiviruses,while the structure analysis further suggested that might have the hypothetical function of coat *** viral particles of PmPV 1 were isometric and approximately 29 nm in *** analysis showed that PmPV1 is closely related to the Gammapartitivirus within the family Partitiviridae,but forming a separate branch with Colletotrichum acutatum RNA virus 1 and Ustilaginoidea virens partitivirus *** is the first report of the full-length nucleotide sequence of a novel virus classified in the genus Gammapartitivirus,infecting *** strain LG915,the causal agent of leaf blight of Curcuma wenyujin.

关键词： Phoma matteuciicola mycovirus Gammapartitivirus Partitiviridae

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Identification of Diverse mycoviruses Through Metatranscriptomics Characterization of the Viromes of Wheat Fusarium Head Blight pathogens

Identification of Diverse Mycoviruses Through Metatranscript...

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中国植物病理学会2016年学术年会

作者： ZHANG Zhong-mei PENG Yun-liang JIANG Dao-hong Institute of Plant Protection Sichuan Academy of Agricultural Sciences/Key Laboratory of Integrated Pest Management on Crops in Southwest ChinaMinistry of Agriculture College of Plant Sciences & Technology of Huazhong Agricultural University

To characterize the viromes of wheat Fusarium Head Blight pathogens,a high-throughput sequencing-based metatranscriptomic approach was used to detect viral *** RNA from mycelia of 150 Fusarium *** isolated from diseased wheat grain from Sichuan province in 2015 was *** data were assembled de novo,and contigs with predicted amino acid sequence similarities to viruses in the non-redundant protein database were *** analysis identified 55 genome segments representing at least 14 mycoviruses,and 11 of them were previously *** novel mycovirus genomes showed similarity to 5 distinct lineages including Mononegavirales,ourmiavirus,Narnaviridae,Gamaflexiviridae and Partitiviridae,respectively.

关键词： Fusarium spp. metatranscriptome mycovirus

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Diversity of ds RNA Viruses Infecting Rice Sheath Blight Fungus Rhizoctonia solani AG-1 IA

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Rice science 2018年第1期25卷 57-60页

作者： ZHENG Li LIU Chen ZHANG Meiling YANG Mei ZHOU Erxun Guangdong Province Key Laboratory of Microbial Signals and Disease Control/College of Agriculture South China Agricultural University

Rice sheath blight,caused by Rhizoctonia solani(Kühn),is a notorious soil-borne disease prevalent in many rice-growing *** several sporadic studies of mycoviruses in *** AG-1 IA have been reported for single strain of *** AG-1 IA,there have been no reports describing the distribution and diversity of mycoviruses in natural *** this study,43 *** AG-1 IA strains collected from different locations in China were examined for the presence of ds RNA elements to confirm the presence of viral *** showed that 16 of the 43 fungal strains(37.2%)contained ds RNAs that can be characterized as ***,the species-specific reverse transcription PCR(RT-PCR)showed ds RNA bands with similar sizes do not always contain the same virus but exist as mixed mycoviral ***,our findings indicate mycoviruses infecting *** AG-1 IA in China are diverse,widespread and universal.

关键词： mycovirus Rhizoctonia solani reverse transcription polymerase chain reaction diversity dsRNA rice sheath blight

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The Complete Genome Sequence of a Novel Fusarium graminearum RNA Virus in a New Proposed Family With the Order Tymovirales

The Complete Genome Sequence of a Novel Fusarium graminearum...

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中国植物病理学会2016年学术年会

作者： CHEN Xiao-guang HE Hao YANG Xiu-fen ZENG Hong-mei QIU De-wen GUO Li-hua State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural Sciences

The complete nucleotide sequence of Fusarium graminearum deltaflexivirus 1(FgDFV1),a novel positive single-stranded(+ ss) RNA mycovirus,was sequenced and *** complete genome of FgDFV1/BJ59 was shown to be 8 246 nucleotides(nts) long excluding the poly(A)***1/BJ59 was predicted to contain a large open reading frame(ORF 1) and four smaller ORFs(2 ).0RF1 encodes a putative replication-associated polyprotein(RP) of 2 042 amino acides(aa)and contains three conserved domains,viral RNA meylthtransferase(Mtr),viral RNA helicase(Hel)and RNA-dependent RNA polymerase(RdRp).ORFs(2 5) encode four putative small hypothetical protein(12 18kDa) with unknown biological *** analysis based on RP sequences indicated that FgDFV1 is phylogenetically related to soybean leaf-associated mycoflexivirus 1(SlaMyfV1)and Sclerotinia sclerotiorum deltaflexivirus 1(SsDFV1),a cluster of an independent group belonging to a newly proposed family Deltaflexiviridae in the order ***,FgDFV1 is markedly different from SsDFV1 and SlaMyfV1 in genome organization and nucleotide ***1 may represent additional species in the new genus Deltaflexivirusor possibly new different genera in the family Deltaflexiviridae.

关键词： Fusarium graminearum FgDFV1 mycovirus

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Phenotypic and transcriptomic analysis of the Fusarium pseudograminearum strain containing a mutant megabirnavirus

Phenotypic and transcriptomic analysis of the Fusarium pseud...

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中国植物病理学会2021年学术年会

作者： LI Ke LIU Dongwei WANG Zhifang XIE Yuan SONG Jiaqing PAN Xin YAN Shuwei GAO Fei ZHANG Xiaoting LI Honglian College of Plant Protection Henan Agricultural University National Key Laboratory of Wheat and Maize Crop Science

Fusarium pseudograminearum is the dominant pathogen of wheat crown rot disease causing serious economic losses in Huanghuai wheat area,China.A megabirnavirus with a bi-segmented dsRNA genome,named FpgMBV1,had been discovered in the hypovirulent strain FC136-2 A of *** this study,we report a progeny strain of FC136-2 A with the genome dsRNA2 of FpgMBV1 lost and designated it as FC136-2 A-Δ*** phenotype and comparative transcriptome of FC136-2 A and FC136-2 A-ΔdsRNA2 were *** showed that the growth rate and sporulation rate were significantly reduced,and the FpgMBVl-dsRNA1 content were significantly strengthened in FC136-2 A-Δ*** for both strains,the pathogenicity to wheat coleoptile were low,meaning that the hypovirulence effect of FpgMBV1 still exists in the strain FC136-2 A-Δ***,a relatively lower capacity to endure 0.3 mol/L CaCl and a higher frequency of mycelial tip dispelling were found in FC136-2 A-ΔdsRNA2 than in FC136-2 A.A total of 4208 genes were found to be differently expressed in FC136-2 A-ΔdsRNA2 comparing to FC136-2 A,with 2082 upregulated and 2126 *** differently expressed genes(DEGs) were involved in pathways including cell apoptosis,transport metabolism,signal transduction,amino acid metabolism,fat metabolism and the biosynthesis of other secondary *** summary,a *** strain with a mutant FpgMBV1 were found and the comparing transcriptome were analyzed to reveal the hypovirulence mechanism underlying the interaction between *** and FpgMBV1,which lay a foundation for deep understanding of the pathogenicity nature of *** and the gene functions of megabirnavirus.

关键词： wheat crown rot Fusarium pseudograminearum mycovirus genome rearrangement

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