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Identification and antibiotic susceptibility evaluation of mycoplasma synoviae isolated from chickens in central China

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Animal Diseases 2023年第3期3卷 202-211页

作者： Chun Wang Naji Zhou Haopeng Lu Rongkun Yang Weitao Cui Qingrong Xu Yuncai Xiao Sishun Hu Rui Zhou Zili Li Zutao Zhou College of Animal Medicine Huazhong Agricultural UniversityWuhan 430070HubeiChina Key Lab of Agricultural Microbiology of State Huazhong Agricultural UniversityWuhan 430070HubeiChina Key Lab of Preventive Veterinary Medicine of Hubei Province Huazhong Agricultural UniversityWuhan 430070HubeiChina JiangSu Ecolovo Group Ltd. Suqian 223833JiangsuChina

mycoplasma synoviae(***)infections have become an increasingly serious concern in China because they cause huge economic losses to the poultry *** treatment is one of control strategies that can be used to contain clinical outbreaks in ***-free flocks,especially because the bacteria can be transmitted through *** understand *** infection status in farms of central China and the antibiotic susceptibility of the circulating strains in vivo and in vitro,485 samples were collected from five provinces from 2019 to ***-two strains were isolated and *** of the minimum inhibitory concentration(MIC)of eight antibiotics(tylvalosin,tiamulin,tilmicosin,lincomycin,enrofloxacin,chlortetracycline,doxycycline and tylosin)for isolates showed that tylvalosin,doxycycline and tiamulin were effective against 52 clinical isolates(MIC values≤0.0625-0.25μg/mL,≤0.0625-1μg/mL,and 0.25-2μg/mL,respectively).Tilmicosin,enrofloxacin and lincomycin had high MICo values(>32μg/mL).An artificial *** infection model was established in chickens for evaluation of the short-term therapeutic effect of these *** 5 days of medication,doxycycline(200 mg/L)showed a superior ability to inhibit *** compared with other groups,as did tylvalosin(200 mg/L).Furthermore,the therapeutic efficacy of tylvalosin(0.4μg/mL)on intra-embryo-injected *** was higher than that of tiamulin at the same dose.A combination of MiC values determined in vitro and therapeutic effects observed in vivo revealed that tylvalosin and doxycycline had the best therapeutic *** also showed better inhibitory effects on the vertical transmis-sion of *** than tiamulin.

关键词： mycoplasma synoviae Antibiotics Minimum inhibitory concentration Therapeutic effect Drug evaluation

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Isolation and Progeny Transmission of Non-Temperature-Sensitive MS-H Vaccine Strains of mycoplasma synoviae from Temperature-Sensitive MS-H-Vaccinated Laying Breeder Hens

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Food and Nutrition Sciences 2023年第7期14卷 579-588页

作者： Victor M. Petrone-Garcia Raquel López-Arellano Inkar Castellanos-Huerta Saeed El-Ashram Ebtesam Al-Olayan Danielle Graham Guillermo Tellez-Isaias Departamento de Ciencias Pecuarias Universidad Nacional Autónoma de México (UNAM) Cuautitlan Izcalli Estado de México México Laboratorio No 5: LEDEFAR Unidad de Investigacion Multidisciplinaria Facultad de Estudios Superi-ores Cuautitlan UNAM Cuautitlan Izcalli Estado de México México Department of Poultry Science University of Arkansas Fayetteville USA Department of Zoology Faculty of Science Kafrelsheikh University Kafr El-Sheikh Egypt Department of Zoology College of Science King Saud University Riyadh Saudi Arabia

This pilot study reports the vertical transmission and reverse thermosensitivity of the MS-H vaccine strain of mycoplasma synoviae (MS) by RAPD in commercial breeders and their progeny. At two weeks of age, breeders were vaccinated with the ts+ MS-H strain. At 9 weeks of age, an outbreak of infectious synovitis (IS) was detected in the progeny. Tracheal swab samples were collected from breeders at 24, 39, 48, and 70 weeks of age. At 9 weeks, pullets swab from the elbow joints were collected. RAPD was performed on the isolates at 39.5°C, and the same ts- MS-H strains were identified in the breeder hens and their progeny. Tracheal swabs from breeder hens were negative to MS isolation at 37°C and 39.5°C at 24- and 39-weeks. MS isolation was recovered from tracheal swabs from 9/10 and 10/10 breeders at 48- and 70- week. At 9 weeks of age in the progeny, MS was isolated from tracheal swabs of 10/10 from non-IS pullets. MS was isolated from 9/10 joints samples. The isolates from breeder hens and their progeny showed non-significant differences in five antimycoplasmic MIC100 values;otherwise, enrofloxacin presented a significant difference in MIC100 value (p < 0.05). This investigation demonstrated the reversal of the thermosensitivity, pathogenicity, and vertical transmission of the MS-H strain. Consequently, it is crucial to contemplate the danger of reversing pathogenicity and transmission to progeny when applying the MS-H vaccine strain.

关键词： Vertical Transmission Thermosensitivity mycoplasma synoviae RAPD Vax-Safe®

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Detection of mycoplasma synoviae Infection in Broiler Breeder Farms of Morocco Using Serological Assays and Real Time PCR

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Journal of Life Sciences 2014年第10期8卷 815-821页

作者： Nassik Saadia Aboukhalid Rachid Azzam Falak Rahmatallah Naoufal Lahlou-Amine Idriss Fassi-FihriOuafaa El Houadfi Mohammed Avian Pathology Unit Department of Pathology and Veterinary Public Health Agronomy and Veterinary Institute Hassan IIRabat 10000 Morocco Immunology and Biochemistry Laboratory Faculty of Sciences Mohamed V-Agdal University Rabat 10000 Morocco University Mohammed V Souissi Mohammed V Military Teaching Hospital Research and Biosafety Level 3 Laboratory Rabat10000 Morocco Microbiology Immunology and Infectious Diseases Unit Department of Pathology and Veterinary Public Health Agronomy andVeterinary Institute Hassan II Rabat 10000 Morocco

mycoplasma synoviae （MS） is an economically important pathogenic agent of chickens, causing air sacculitis and synovitis. The diagnosis and monitoring of M. synoviae infection is usually made using serological assays, while confirmative diagnosis is made by isolation and identifcation of the organism, because of the cross reaction between M. gallisepticum and M. synoviae. This study was conducted from 2011 to 2013 during which l l broiler breeder flocks were sampled. These farms were located in all regions of morocco, The sampling was conducted as follows： Farms were visited on day one old chicks ＂day of importation from Europe＂. 20 to 60 ＂chicks＂ were randomly sampled. At the age of 8, 16, 32 and 56 weeks, 60 blood samples and 60 tracheal swabs were collected at each sampling. The serological screening was performed using Rapid Slide Agglutination （RSA） according the OIE protocol and Indirect EL1SA （IDEXX） according the manufacturer＇s instructions. The molecular diagnosis was performed using a commercial kit of a duplex real time PCR （Life Biotechnology）. The results revealed that one day old chicks were negative to MS by RSA and PCR, however they have a variable stock of maternal antibodies （Ig-Y） detected by iELISA. The seroprevalence found by RSA is variable and increase with the age （Sth week： 55%, 16th week： 91%, 32tb and 56th week： 100%）, the same profile was traced by PCR （Sth week： 36%, 16th week： 64%, 32th week： 82%, 56th week： 100%）, however, all farms were positive by iELISA, from the first day to 56th weeks. These results show that MS infections are very frequent and very widespread among poultry breeder flocks, and showed a perfect agreement between serological tests and Real time PCR starting from 32th week of age.

关键词： mycoplasma synoviae Rapid Slide Agglutination iEL1SA PCR broiler breeders flocks

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THE DETECTION OF mycoplasma GALLISEPTICUM AND mycoplasma synoviae BY MULTIPLEX PCR

THE DETECTION OF MYCOPLASMA GALLISEPTICUM AND MYCOPLASMA SYN...

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面向21世纪的动物科学与动物医学国际研讨会

作者： XIE Zhizun PANG Yaoshang EENG Xianwen LIU Jiabo XIE Zhiqin (Guangxi Veterinary Research Institute Nannign 530001)

＜正＞Two set of specific primers were designed and synthesized according to the sequences of mycoplasma gallisepticum（MG） and mycoplasma synoviae（MS）. A multiplex PCR was optimized to simultaneously detect two species of mycoplasma MG and MS. Two specific bands, 732bp（MS） and 207bp（MS） DNA products were amplified from the samples which simultaneously contained MG and MS DNA, but not from other eight avian pathogenic virus and bacteria. As little as 100fg of MG and MS DNA were detected using gel electrophoresis in this multiplex PCR. Using our project in this article, one PCR can detect two

关键词： Multiplex PCR mycoplasma gallisepticam mycoplasma synoviae

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