检索结果-南通市图书馆

Improving the biotransformation efficiency of soybean phytosterols in mycolicibacterium neoaurum by the combined deletion of fbpC3 and embC in cell envelope synthesis

引用

Synthetic and Systems Biotechnology 2022年第1期7卷 453-459页

作者： Liang-Bin Xiong Hao-Hao Liu Lu Song Miao-Miao Dong Jie Ke Yong-Jun Liu Ke Liu Ming Zhao Feng-Qing Wang Dong-Zhi Wei Jiading District Central Hospital Affiliated Shanghai University of Medicine and Health Sciences Shanghai201800PR China State Key Laboratory of Bioreactor Engineering Newworld Institute of BiotechnologyEast China University of Science and TechnologyShanghai200237PR China Huawei Safety Evaluation&Medical Research(Shanghai)Co. Ltd.Shanghai201206PR China

Biotransformation of soybean phytosterols into 9α-hydroxy-4-androstene-3,17-dione(9-OHAD)by mycobacteria is the core step in the synthesis of adrenocortical ***,the low permeability of the dense cell envelope largely inhibits the overall conversion efficiency of *** antigen 85(Ag85)complex encoded by fbpA,fbpB,and fbpC was proposed as the key factor in the combined catalysis of mycoloyl for producing mycolyl-arabinogalactan(m-AG)and trehalose dimycolate(TDM)in mycobacterial cell ***,we confirmed that fbpC3 was essential for the biotransformation of trehalose monomycolate(TMM)to TDM in mycolicibacterium *** deficiency of this gene raised the cell permeability,thereby enhancing the steroid uptake and *** 9-OHAD yield in the fbpC3-deficient 9-OHAD-producing strain was increased by 21.3%.Moreover,the combined deletion of fbpC3 and embC further increased the 9-OHAD yield compared to the single deletion of ***,after 96 h of bioconversion in industrial resting cells,the 9-OHAD yield of 11.2 g/L was achieved from 20 g/L phytosterols and the productivity reached 0.116 g/L/*** summary,this study suggested the critical role of the fbpC3 gene in the synthesis of TDM in *** and verified the feasibility of improving the bioconversion efficiency of phytosterols through the cell envelope en-gineering strategy.

关键词： mycolicibacterium 9-OHAD Cell permeability Soybean phytosterol fbpC3 embC

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Multiplexed site-specific genome engineering in mycolicibacterium neoaurum by Att/Int system

引用

Synthetic and Systems Biotechnology 2022年第3期7卷 1002-1011页

作者： Ke Liu Gui-Hong Lin Kun Liu Yong-Jun Liu Xin-Yi Tao Bei Gao Ming Zhao Dong-Zhi Wei Feng-Qing Wang State Key Laboratory of Bioreactor Engineering Newworld Institute of BiotechnologyEast China University of Science and TechnologyShanghai200237China

Genomic integration of genes and pathway-sized DNA cassettes is often an indispensable way to construct robust and productive microbial cell *** some uncommon microbial hosts,such as mycolicibacterium and Mycobacterium species,however,it is a ***,we present a multiplexed integrase-assisted site-specific recombination(miSSR)method to precisely and iteratively integrate genes/pathways with controllable copies in the chromosomes of Mycolicibacteria for the purpose of developing cell ***,a single-step multi-copy integration method was established in *** by a combination application of mycobacteriophage L5 integrase and two-step allelic exchange strategy,the efficiencies of which were~100%for no more than three-copy integration events and decreased sharply to~20%for five-copy integration ***,the R4,Bxb1 andΦC31 bacteriophage Att/Int systems were selected to extend the available integration toolbox for multiplexed gene integration ***,a reconstructed mycolicibacterial Xer recombinases(Xer-cise)system was employed to recycle the selection marker of gene recombination to facilitate the iterative gene *** a proof of concept,the biosynthetic pathway of ergothioneine(EGT)in mycolicibacterium neoaurum ATCC 25795 was achieved by remodeling its metabolic pathway with a miSSR *** six copies of the biosynthetic gene clusters(BGCs)of EGT and pentose phosphate isomerase(PRT),the titer of EGT in the resulting strain in a 30 mL shake flask within 5 days was enhanced to 66 mg/L,which was 3.77 times of that in the wild *** improvements indicated that the miSSR system was an effective,flexible,and convenient tool to engineer the genomes of Mycolicibacteria as well as other strains in the Mycobacteriaceae due to their proximate evolutionary relationships.

关键词： Site-specific recombination Phage integrase Xer recombinases mycolicibacterium Multi-copy integration

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：