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Antimicrobial Activity and mutant prevention concentration of Fusidic Acid against Clinical Isolates of Staphylococcus

Antimicrobial Activity and Mutant Prevention Concentration o...

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第十三次全国临床药理学学术大会

作者： Jing Ouyang Yong-Chuan Chen*Pei-Yuan Xia Li-Rong Xiong Lin-Li Xie Feng-Jun Sun

To prevent or reduce the development of drug resistance and provide guidance for the clinical treatment of staphylococcal infections,fusidic acid was studied to determine its in vitro activities and mutant prevention concentration(MPC) against clinical isolates of *** the MPC is reported herein for the first time. The minimum inhibitory concentrations(MIC) of fusidic acid and other antibiotics for 203 strains of staphylococci were determined by the agar dilution *** effects of fusidic acid combined with other antibiotics were determined by the checkerboard method,and the results were interpreted using the fractional inhibitory concentration *** determine the MPC of fusidic acid against staphylococci,10CFU of bacteria was exposed to different concentrations of antibiotics,and the lowest concentrations that completely inhibited bacterial growth were defined as the *** MIC values of fusidic acid against methicillin-resistant Staphylococcus aureus(MRSA),methicillin-susceptible Staphylococcus aureus(MSSA),methicillin-resistant coagulase-negative staphylococci(MRCNS) and methicillin-susceptible coagulase-negative staphylococci (MSCNS) were 1,1,8 and lmg/L,respectively,and the resistance rates were 6.35,0,26.32 and 5.26%, *** rates of synergism for fusidic acid with vancomycin,moxifloxacin and meropenem were 55.0, 33.3 and 66.7%,respectively;no antagonism was *** MPC90 values of fusidic acid against MRSA and MSSA were both 16 mg/L,and the MPC90/MIC50 ratios were 16 and 8,*** rate of fusidic acid resistance among clinical isolates of Staphylococcus is relatively low,and fusidic acid may play a significant role in preventing the development of resistance mutations in ***,clinicians should avoid using a small single dose of fusidic acid in the treatment of staphylococcal infections.

关键词： fusidic acid Staphylococci mutant prevention concentration fractional inhibitory concentration index

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Comparative mutant prevention concentrations of Linezolid and Vancomycin against Methicillin-Resistant Staphylococcus aureus and Characterization of the Selected Reduced Vancomycin Susceptibility Strains

Comparative Mutant Prevention Concentrations of Linezolid an...

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第二届中国临床微生物学大会暨微生物学与免疫学三江论坛

作者： Jian-hong Zhao Jing Li Su-ju Sun Hongbo Zhao Dong-yan Shi Ji-hong Li Xing Wang Xi Lu Xiao-lei Liu Wen-jie Song Qiang Ye Shu-min Zhang and Lei Shi Department of Clinical Laboratory Sciences Second Hospital of Hebei Medical University College of Public Health Hebei Medical University College of Light Industry and Food Sciences South China University of Technology Chinese National Institute for the Control of Pharmaceutical and Biological Products(NICPBP)

This study aimed to compare the ability of linezolid and vancomycin to prevent the clinical methicillin-resistant Staphylococcus aureus（MRSA）resistant mutant by determining mutant prevention concentration（MPC）.We further investigated the possible molecular mechanism associated with reduced vancomycin susceptibility in the mutant strains by molecular fingerprinting ***-six clinical MRSA isolates were enriched in broth,and the bacterial concentration was adjusted to 1010 CFU/mL for MPC ***-type strains and mutant strains selected by vancomycin during MPC determination were characterized by pulsed-field gel electrophoresis（PFGE）,Sau-PCR assay,denaturing gradient gel electrophoresis（DGGE）,scanning electron microscopy（SEM）,and transmission electron microscopy（TEM）.Our results showed that both linezolid and vancomycin might have great ability to restrict the selection of resistant mutants of *** had a narrower mutant selection window against the MRSA isolates than ***-PCR assay and PFGE revealed that pre-scanning and post-scanning strains selected by vancomycin during the experimental process of MPC had different DNA *** and SEM showed that the mutant strains had slightly thicker cell walls than the wild-type *** was used to probe for mutations in the graS gene,but no different banding patterns were observed on the electrophoretogram *** showed greater ability to restrict the selection of resistant mutants of MRSA than *** wild-type strains and mutant strains selected by vancomycin during the experimental process of MPC,there may be mutations in genes other than the graS gene or existence of other resistant mechanisms.

关键词： mutant prevention concentration Linezolid Vancomycin Methicillin-Resistant Staphylococcus aureus

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