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Pre-and post-zygotic reproductive isolation between co-occurring mussaenda pubescens *** and M. shikokiana(Rubiaceae)

Journal of Integrative Plant Biology 2014年第4期56卷 411-419页

作者： Shi Chen Zhonglai Luo Dianxiang Zhang Key Laboratory of Plant Resources Conservation and Sustainable Utilization South China Botanical Garden the Chinese Academy of Sciences

Reproductive isolation is a fundamental require- ment for speciation and includes several sequential stages. Few studies have determined the relative contributions of pre- and post-zygotic reproductive isolation in plants, especially between relative species with clear differentiation in flower form. To investigate the mechanisms responsible for repro- ductive isolation in sympatric A4ussaenda pubescens var. alba and mussaenda shikokiana （Rubiaceae） in Guangxi Province, China, we made observations of flowering phenology, patterns of insect visitation, and conducted pollination experiments, including artificial hybridization. The two species had over- lapping flowering times and were pollinated by overlapping pollinators; however, their relative importance differed significantly with M. pubescens visited more commonly by bees and M. shikokiana more frequently by butterflies. Using vegetative and floral characters and molecular evidence based on nuclear ribosomal internal and external transcribed spacer regions we detected seven naturally occurring hybrids among a sample of approximately 125 individuals. Hybrids were characterized by morphologies that most closely resembled their maternal parents based on chloroplast evidence. Studies of artificially synthesized and natural hybrids demonstrated that hybrid seed had very low germination rates and naturally occurring hybrids exhibited pollen sterility. Post-zygotic reproductive isolating mechanisms play a primary role in limiting gene exchange between co-occurring species and maintaining species integrity in areas of sympatry.

关键词： Gene flow mussaenda natural hybrids reproductiveisolation sympatric congeners

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Reproductive isolation between sympatric sister species, mussaenda kwangtungensis and M. pubescens var. alba

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Journal of Integrative Plant Biology 2015年第10期57卷 859-870页

作者： Zhonglai Luo Tingting Duan Shuai Yuan Shi Chen Xiufeng Bai Dianxiang Zhang Key Laboratory of Plant Resources Conservation and Sustainable Utilization South China Botanical Garden the Chinese Academy of Sciences University of the Chinese Academy of Sciences Beneficial Insects Institute Fujian Agriculture and Forestry University Department of Cell Biology the Third Military Medical University

Reproductive isolation defines the biological species concept and plays a key role in the formation and maintenance of species. The relative contributions of different isolating stages has been suggested to be closely associated with phylogenetic relatedness. Few studies have focused on the relative contributions of pre- versus post-zygotic mechanisms, and even fewer have been conducted under strict phylogenetic frameworks. Pre-and post-zygotic reproductive isolation stages have been investigated in the sister species mussaenda kwangtungensis and M. pubescens var. alba. The two species have partly overlapping distribu-tion ranges and flowering times, while the principal pollinators differed strikingly for them, demonstrating strong pre-zygotic isolations. Natural hybrids were detected by simple sequence repeat markers and their maternal parents were identified based on chloroplast gene sequen-ces. Five out of 81 individuals were suggested to be hybrids that fall into the categories F2, BC1, and BC2 by the NewHybrids analysis. Interspecific crossings resulted in significantly reduced fruit set and seed germination rates. Phylogenetic analysis revealed short Kimura-2-parameter distance between M. kwangtungensis and M. pubescens var. alba. These findings strongly supported the hypothesis that for species with a closer phylogenetic relationship, pre-zygotic isolation plays an important part in limiting gene exchange in sympatric areas.

关键词： Hybridization microsatellites mussaenda phylogenetic analysis reproductive isolation

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Determination of Chlorogenic Acid in Stems of mussaenda pubescens *** High Performance Liquid Chromatography

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Agricultural Biotechnology 2018年第6期7卷 205-207页

作者： Qianhua ZHANG Dongping TU Lichun ZHAO Yi LUO Songhua HE Tao ZHU College of Pharmacy Guangxi University of Chinese MedicineNanning 530200China Guangxi Zhuang Yao Medicine Center of Engineering and Technology Nanning 530200China Guangxi Institute For Food and Drug Control Nanning 530021China

[Objectives] This study was conducted to establish a method for determination of chlorogenic acid in mussaenda pubescens Ait.f.[Methods]HPLC used SHISEIDO C_(18) MG Ⅱ column( 5 μm,4.6 mml.D.× 250 mm) as chromatographic column and acetonitrile-0.4% phosphoric acid solution( 9∶91) as mobile *** separation was performed at a flow rate of 1.0 ml/min and a column temperature of 30 ℃,and the detection wavelength was set at 327 nm.[Results]Chlorogenic acid had a good linear relation in the range of 0.254 7-2.547 5 μg( R2= 0.999 9).The recovery rate was 97.8%,RSD = 1.82%( n = 9).[Conclusions]The content of chlorogenic acid in *** was determined by ultrasonic extraction and *** method was simple,stable and reliable and could be used for the quality control of ***.

关键词： Chlorogenic acid HPLC Content determination mussaenda pubescens Ait.f.

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