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Direct-writing Process and in vivo Evaluation of Prevascularized Composite Constructs for muscle tissue engineering Application

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Journal of Bionic engineering 2020年第3期17卷 457-468页

作者： Qin Lian Tingze Zhao Tian Jiao Yige Huyan Heng Gu Lin Gao State Key Laboratory for Manufacturing System engineering Xi'an Jiaotong UniversityXi'an 710049China Health Science Center Xi'an Jiaotong Universityy Xi'an 710061China

tissue engineered skeletal muscle is expected to treat muscle defects caused by trauma and ***,designing and manufacturing thick and complex tissue engineered skeletal muscle requires vascularization to ensure its internal cell viability and nutrient supply in *** this article,we developed a set of Direct-Writing(DW)bio-printing procedure to manufacture a prevascularized composite construct with Human Umbilical Vein Endothelial Cell(HUVEC)and C2C12 cells for muscle tissue engineering *** put the cells into the construct during the DW process to obtain the prevascularization and intend to promote its vascularization in vivo *** constructs with cells or without cells were implanted respectively into nude mice back for 3 weeks,after which the mice healthily live for all the time and all the implants are tightly bonded to the *** immunohistochemical analysis,CD31-positive blood vessels existed in the implanted samples with cells are more substantial than those without cells,but the implanted samples with HUVEC and C2C12 cells have much more number of small blood vessels distributing ***,the implants with cells,especially that with HUVEC and C2C12 cells,are able to get better fusion with the host skin and subcutaneous *** analysis demonstrates that our DW-based constructs have the potential to be getting to vascularize the tissue engineered muscle.

关键词： DW muscle tissue engineering in vivo experiment prevascularization

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Cartilage and facial muscle tissue engineering and regeneration： a mini review

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Bio-Design and Manufacturing 2018年第2期1卷 115-122页

作者： Michael Del Monico Mohammadreza Tahriri Mina D. Fahmy Hamed Ghassemi Daryoosh Vashaee Lobat Tayebi Marquette University School of Dentistry Milwaukee WI 53233 USA Eye Research Center Farabi Eye Hospital Tehran University of Medical Sciences Tehran Iran Department of Electrical and Computer engineering North Carolina State University Raleigh NC 27606 USA

Cartilage and facial muscle tissue provide basic yet vital functions for homeostasis throughout the body, making human survival and function highly dependent upon these somatic components. When cartilage and facial muscle tissues are harmed or completely destroyed due to disease, trauma, or any other degenerative process, homeostasis and basic body functions consequently become negatively affected. Although most cartilage and cells can regenerate themselves after any form of the aforementioned degenerative disease or trauma, the highly specific characteristics of facial muscles and the specific structures of the cells and tissues required for the proper function cannot be exactly replicated by the body itself. Thus, some form of cartilage and bone tissue engineering is necessary for proper regeneration and function. The use of progenitor cells for this purpose would be very beneficial due to their highly adaptable capabilities, as well as their ability to utilize a high diffusion rate, making them ideal for the specific nature and functions of cartilage and facial muscle tissue. Going along with this, once the progenitor cells are obtained, applying them to a scaffold within the oral cavity in the affected location allows them to adapt to the environment and create cartilage or facial muscle tissue that is specific to the form and function of the area. The principal function of the cartilage and tissue is vascularization, which requires a specific form that allows them to aid the proper flow of bodily functions related to the oral cavity such as oxygen flow and removal of waste. Facial muscle is also very thin, making its reproduction much more possible. Taking all these into consideration, this review aims to highlight and expand upon the primary benefits of the cartilage and facial muscle tissue engineering and regeneration, focusing on how these processes are performed outside of and within the body.

关键词： Soft tissue regeneration Cartilage tissue engineering muscle tissue engineering Facial regeneration

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Preparation and Endothelialization of Multi-level Vessel-like Network in Enzymated Gelatin Scaffolds

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Journal of Bionic engineering 2018年第4期15卷 673-681页

作者： Guirong Dong Qin Lian Laixia Yang Wei Mao Shiyang Liu Chao Xu School of Mechanical engineering Xi' an University of Science and Technology Xi' an 710054 China Faculty of Higher Vocational and Technical Education Xi' an University of Technology Xi' an 710082 China State Key Laboratory for Manufacturing Systems engineering Xi' an Jiao Tong University Xi' an 710049 China

Loss of function of large tissues is an urgent clinical problem. Although the artificial microfluidic network fabricated in large tis- sue-engineered constructs has great promise, it is still difficult to develop an efficient vessel-like design to meet the requirements of the biomimetic vascular network for tissue engineering applications. In this study, we used a facile approach to fabricate a branched and multi-level vessel-like network in a large muscle scaffolds by combining stereolithography （SL） technology and enzymatic crosslinking mechanism. The morphology of microchannel cross-sections was characterized using micro-computed tomography. The square cross-sections were gradually changed to a seamless circular microfluidic network, which is similar to the natural blood vessel. In the different micro-channels, the velocity greatly affected the attachment and spread of Human Umbilical Vein Endothelial Cell （HUVEC）-Green Fluorescent Protein （GFP）. Our study demonstrated that the branched and multi-level microchannel network simulates biomimetic microenvironments to promote endothelialization. The gelatin scaffolds in the circular vessel-like networks will likely support myoblast and surrounding tissue for clinical use.

关键词： large-size scaffolds vascular network enzymaticaUy gelatin hydrogel endothelialization muscle tissue engineering

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