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Factors influencing myogenic differentiation of adipose-derived stem cells and their application in muscle regeneration

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Chinese Journal Of Plastic and Reconstructive Surgery 2022年第3期4卷 126-132页

作者： Na Liu Guanhuier Wang Yonghuan Zhen Yujia Shang Fangfei Nie Lingjuan Zhu Zhenmin Zhao Yang An Department of Plastic Surgery Peking University Third HospitalBeijing 100191China School of Traditional Chinese Materia Medica Key Laboratory of Structure-Based Drug Design&Discovery of Ministry of EducationShenyang Pharmaceutical UniversityShenyang 110016China

Skeletal muscle regeneration mainly depends on muscle satellite cells;however,these cells are not sufficient for supporting repair and regeneration in volumetric muscle loss(VML),Duchenne muscular dystrophy,and other muscle injuries or muscle *** such,much work has been conducted in recent years to search for myogenic stem ***-derived stem cells(ADSCs)have a wide range of sources,rapid growth,and multi-directional differentiation potential,and have become vital candidates for muscle *** factors influence the myogenic differentiation capacity of *** paper reviews the regulatory aspects and possible factors that have been identified in recent years to affect myogenic differentiation of *** on these factors,gene editing,and perfusion concepts,a method was proposed to achieve maximal differentiation efficiency of *** study focused on the application of ADSCs in muscle regeneration and *** on the importance of myogenic differentiation of ADSCs for the repair and regeneration of muscle damage,this study provides a basis for future research surrounding the efficient induction of myogenic differentiation of ADSCs in vitro.

关键词： Adipose-derived stem cells muscle regeneration muscle diseases Myogenic differentiation Regulatory factors

Effects of PGC-1αoverexpression on the myogenic response during skeletal muscle regeneration

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Sports Medicine and Health Science 2022年第3期4卷 198-208页

作者： Tyrone A.Washington Wesley S.Haynie Eleanor R.Schrems Richard A.Perry Jr. Lemuel A.Brown Breanna M.Williams Megan E.Rosa-Caldwell David E.Lee Jacob L.Brown Exercise muscle Biology Laboratory Exercise Science Research CenterDepartment of HealthHuman Performance and RecreationUniversity of ArkansasFayettevilleAR72701USA Cachexia Research Laboratory Exercise Science Research CenterDepartment of HealthHuman Performance and RecreationUniversity of ArkansasFayettevilleAR72701USA

The ability of skeletal muscle to regenerate from injury is crucial for locomotion,metabolic health,and quality of *** proliferator-activated receptor-γcoactivator-1α(PGC1A)is a transcriptional coactivator required for mitochondrial *** mitochondrial biogenesis is associated with improved muscle cell differentiation,however PGC1A's role in skeletal muscle regeneration following damage requires further *** purpose of this study was to investigate the role of skeletal muscle-specific PGC1A overexpression during regeneration following damage.22 C57BL/6J(WT)and 26 PGC1A muscle transgenic(A1)mice were injected with either phosphate-buffered saline(PBS,uninjured control)or Bupivacaine(MAR,injured)into their tibialis anterior(TA)muscle to induce skeletal muscle *** muscles were extracted 3-or 28-days postinjury and analyzed for markers of regenerative myogenesis and protein ***1a mRNA was~10–20 fold greater in A1 *** of protein synthesis,AKT and 4EBP1,displayed decreases in A1 mice compared to WT at both timepoints indicating a decreased protein synthetic *** mRNA was~75%lower compared to WT 3 days *** mice exhibited decreased cross-sectional area of the TA muscle at 28 days post-injection with bupivacaine compared to all other ***1A overexpression modifies the myogenic response during regeneration.

关键词： PGC-1α Skeletal muscle muscle regeneration Satellite cells Protein turnover p38 MAPK

Research Progress of Vitamins on muscle regeneration

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Journal of Northeast Agricultural University(English Edition) 2022年第4期29卷 91-96页

作者： Su Jing-yan Li Ping Wen Xin Zhang Wen-yu Tong Hui-li Key Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang Province Northeast Agricultural UniversityHarbin 150030China Laboratory of Cell and Developmental Biology School of Life SciencesNortheast Agricultural UniversityHarbin 150030China

muscle satellite cells,as muscle stem cells,play a critical role in the process of muscle regeneration,and effective muscle regeneration helps to restore muscle function and maintain the homeostasis of muscle *** damaged muscle,muscle satellite cells are activated to form new myofibers through the process of cell proliferation,migration,differentiation and fusion to complete muscle tissue ***,this process is mainly affected by endogenous gene expression and many exogenous *** in recent years have shown that vitamins,as important nutrients,play an extremely important role in the process of muscle ***,this article reviewed the roles of vitamins in the regeneration of muscle satellite cells,according to the latest research *** would provide more theoretical and data support for the regeneration and repair of muscle damage,muscle atrophy and other muscle diseases,so that it could be better applied in the field of muscle regeneration researches and serve human health.

关键词： vitamin muscle satellite cell muscle regeneration

Accelerated regeneration of the skeletal muscle in RNF13-knockout mice is mediated by macrophage-secreted IL-4/IL-6

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Protein & Cell 2014年第3期5卷 235-247页

作者： Jiao Meng Xiaoting Zou Rimao Wu Ran Zhong Dahai Zhu Yong Zhang The National Laboratory of Medical Molecular Biology Institute of Basic Medical Sciences Chinese Academy of MedicalSciences and School of Basic Medicine Peking Union Medical College Beijing 100005 China

RING finger protein 13 （RNF13） is a newly identified E3 ligase reported to be functionally significant in the reg- ulation of cancer development, muscle cell growth, and neuronal development. In this study, the function of RNF13 in cardiotoxin-induced skeletal muscle regener- ation was investigated using RNF13-knockout mice. RNF13-/- mice exhibited enhanced muscle regeneration --characterized by accelerated satellite cell proliferation --compared with wild-type mice. The expression of RNF13 was remarkably induced in macrophages rather than in the satellite cells of wild-type mice at the very early stage of muscle damage. This result indicated that inflammatory cells are important in RNF13-mediated satellite cell functions. The cytokine levels in skeletal muscles were further analyzed and showed that RNF13＂i＂ mice produced greater amounts of various cytokines than wild-type mice. Among these, IL-4 and IL-6 levels significantly increased in RNF13-/＂ mice. The accelerated muscle regeneration phenotype was abrogated by inhibiting IL-4/IL-6 action in RNF13＂/- mice with blocking antibodies. These results indicate that RNF13 deficiency promotes skeletal muscle regeneration via the effects on satellite cell niche mediated by IL-4 and IL-6.

关键词： RNF13 muscle regeneration satellite celniche. IL-4/IL-6

miR-378-mediated glycolytic metabolism enriches the Pax7^(Hi) subpopulation of satellite cells

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Cell regeneration 2022年第1期11卷 109-118页

作者： Hu Li Lin Kang Rimao Wu Changyin Li Qianying Zhang Ran Zhong Lijing Jia Dahai Zhu Yong Zhang The Max-Planck Center for Tissue Stem Cell Research and Regenerative Medicine Bioland Laboratory(Guangzhou Regenerative Medicine and Health Guangdong Laboratory)Guangzhou 510005China The State Key Laboratory of Medical Molecular Biology Institute of Basic Medical SciencesChinese Academy of Medical Sciences and School of Basic MedicinePeking Union Medical CollegeBeijing 100005China Department of Endocrinology the Second Clinical Medical College of Jinan UniversityShenzhen People’s HospitalShenzhen 518020China

Adult skeletal muscle stem cells,also known satellite cells(SCs),are a highly heterogeneous population and reside between the basal lamina and the muscle fiber *** function as an immediate niche to support SC self-renewal and activation during muscle growth and ***,we demonstrate that microRNA 378(miR-378)regulates glycolytic metabolism in skeletal muscle fibers,as evidenced by analysis of myofiber-specific miR-378 transgenic mice(TG).Subsequently,we evaluate SC function and muscle regeneration using miR-378 TG *** demonstrate that miR-378 TG mice significantly attenuate muscle regeneration because of the delayed activation and differentiation of ***,we show that the miR-378-mediated metabolic switch enriches Pax7^(Hi) SCs,accounting for impaired muscle regeneration in miR-378 TG ***,our data suggest that miR-378 targets the Akt1/FoxO1 pathway,which contributes the enrichment of Pax7^(Hi) SCs in miR-378 TG ***,our findings indicate that miR-378 is a target that links fiber metabolism to muscle stem cell heterogeneity and provide a genetic model to approve the metabolic niche role of myofibers in regulating muscle stem cell behavior and function.

关键词： satellite cells muscle regeneration miRNAs glycolytic metabolism

miR-130b inhibits proliferation and promotes differentiation in myocytes via targeting Sp1

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Journal of Molecular Cell Biology 2021年第6期13卷 422-432页

作者： Yu-Cheng Wang Xiaohan Yao Mei Ma Huihui Zhang Hui Wang Lei Zhao Shengnan Liu Chao Sun Peng Li Yuting Wu Xihua Li Jingjing Jiang Yuying Li Yan Li Hao Ying Shanghai Xuhui District Central Hospital Zhongshan-Xuhui HospitalFudan UniversityShanghai 200001China CAS Key laboratory of Nutrition Metabolism and Food SafetyShanghai Institute of Nutrition and HealthChinese Academy of SciencesShanghai 200031China Department of Neuromuscular Disease Children’s Hospital of Fudan UniversityShanghai 201102China Department of Endocrinology and Metabolism Zhongshan HospitalFudan UniversityShanghai 200031China State Key Laboratory of Food Science and Technology School of Food Science and TechnologyJiangnan UniversityWuxi 214122China Key Laboratory of Food Safety Risk Assessment Ministry of HealthBeijing 100021China

muscle regeneration after damage or during myopathies requires a fine cooperation between myoblast proliferation and myogenic differentiation.A growing body of evidence suggests that microRNAs play critical roles in myocyte proliferation and differentiation ***,the molecular mechanisms underlying the orchestration are not fully ***,we showed that miR-130b is able to repress myoblast proliferation and promote myogenic differentiation via targeting Sp1 transcription ***,overexpression of miR-130b is capable of improving the recovery of damaged muscle in a freeze injury ***,miR-130b expression is declined in the muscle of muscular dystrophy ***,these results indicated that miR-130b may play a role in skeletal muscle regeneration and myopathy ***,our findings suggest that the miR-130b/Sp1 axis may serve as a potential therapeutic target for the treatment of patients with muscle damage or severe myopathies.

关键词： miR-130b muscle regeneration proliferation differentiation Sp1

The Effect of Increasing mTOR Signaling on the Speed of regeneration in D. dorotocephala planaria

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Open Journal of Regenerative Medicine 2023年第3期12卷 125-132页

作者： Ammar Danish Freehold High School Freehold USA

muscle strains are a common injury that can occur during physical activity or exercise, and they can range from mild to severe depending on the extent of the damage. The mTOR pathway is a highly conserved signaling pathway that has been implicated in various cellular processes, including tissue regeneration. Previous studies that have investigated protein synthesis in mice have concluded that the mTOR pathway can improve muscle regeneration. However, the specific effects of mTOR pathway activation on muscle regeneration in planarians have yet to be fully explored. Therefore, this study aimed to investigate the use of Arginine and Leucine to stimulate the mTOR pathway for muscle strains in planarians. During the experiment, planarians were amputated, and different dosages of Arginine and Leucine were used to stimulate the mTOR pathway in Dugesia dorotocephala. The speed of muscle regeneration was measured over 14 days in five groups, with thirty planarians in each group. The results showed that the rate of regeneration from 0.134 mM Leucine solution showed a significant increase compared to the control group, while the groups exposed to 0.1, 0.3 mM Arginine, and 0.0134 Leucine did not show significant changes in muscle regeneration. These findings suggest that mTOR pathway activation may enhance muscle regeneration in planarians and that the effects may be dose-dependent. These findings have important implications for developing new treatments for tissue damage. Further studies are needed to fully understand the mechanisms of mTOR pathway activation on muscle regeneration in planarians and its potential use in tissue engineering and regenerative medicine.

关键词： muscle regeneration muscle Strains mTOR Pathway Leucine Arginine

Membrane and Subcellular muscle Injury Are Induced by Single or Multiple Exposure to Cigarette Smoke

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Open Journal of Pathology 2014年第3期4卷 125-137页

作者： Ana Maria Fleig-Mayer Alba Ramirez-Sarmiento Amanda Iglesias Montserrat Morla Carlos Coronell Diego Rodríguez Simona Pichinni Josep Lloreta Piergiorgio Zuccaro Xavier Busquets Joaquim Gea Jose R.Jardim Mauricio Orozco-Levi Centro Universitario do Triangulo(UNITRI) Avenida nicomedes Alves Dos Santos4545-GaveaUberlandiaPulmonary Rehabilitation CenterRespiratory DivisionFederal University of Sao PauloSao PauloBrazil Group of Research in Injury Immune Response and Function(LIF)Municipal Institute of Medical Research(IMIM)Carrer Dr.Aiguader 88BarcelonaE-08003Spain Servei de Pneumologia Hospital del MarPasseig Maritim 25BarcelonaE-08003Spain Centro en Red de Investigacion de Enfermedades Respiratorias(CIBERES) Recinto Hospital Joan March Carretera Soller Km.1207110BunyolaMallorcaIlles BalearsSpain Institut Universitari d’Investigacio en Ciencies de la Salut(IUNICS)and Department de Biologia Universitat Illes Balears(UIB)Cra de ValldemossaKm 7.5PalmaIlles BalearsSpain Laboratorio Di Biochimica Clinica Istituto Superiore Di Sanita.Viale Regina Elena 29900161RomaItaly Respiratory Department Fundacion Cardiovascular de ColombiaCalle 155A 23-58FloridablancaSantanderColombia Servei d’Anatomia Patologica Hospital del MarPasseig Maritim 25BarcelonaE-08003Spain

Cigarette smoking is the main cause of chronic obstructive pulmonary disease (COPD). Diaphragm injury is observed in patients with COPD. However, the potential role of smoking in triggering or perpetuating muscle injury is unknown. The present study was aimed at evaluating the potential role of commercial tobacco smoke as a direct cause of skeletal muscle injury in experimental conditions. Seventy Wistar rats (170 - 250 g) were assigned to smoking (n = 49) or non-smoking (n = 21) groups. The smoking groups were submitted to a single or multiple (i.e., five or thirty) daily sessions of cigarette smoking in an inhalatory chamber (time length: 2 h each session). The level of exposure was constant and assessed by CO concentrations (50 ppm) and serum cotinine analysis. Animals submitted to a single smoke exposure and the corresponding controls were euthanized in groups at 0 h, 2 h, 4 h, 24 h or 48 h after completing the exposure. Animals submitted to multiple exposures were euthanized at 0 h after smoking. Samples from vastus lateralis muscle were obtained and processed for assessing cell injury and selected protein expression. Monoclonal anti-albumin antibodies were used to identify muscle fibers with sarcolemmal (membrane) injury. Subcellular muscle injury was assessed using transmission electron microscopy (EM). MyoD, myogenin and α-tubulin were immunodetected using western blot techniques. Exposure to cigarette smoke associated with significant membrane damage (mean relative difference (MRD) with controls: +181%, p = 0.004) and sarcomere disruptions (MRD: +226%, p = 0.001). Expression of MyoD and myogenin (normalized to α-tubulin) were significantly increased at 4 h and remained increased at 48 h post-exposure. We conclude that not only a single but also consecutive exposure to tobacco smoke have acute deleterious effects on peripheral muscle structure. A rapid induction of subrogate markers of skeletal muscle stress and repair processes associates to sarcolemmal and

关键词： Skeletal muscle muscle Injury muscle regeneration Quadriceps Cigarette Smoking

Sema4C participates in myogenic differentiation in vivo and in vitro through the p38 MAPK pathway

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Sema4C participates in myogenic differentiation in vivo and ...