检索结果-南通市图书馆

Membrane and Subcellular muscle injury Are Induced by Single or Multiple Exposure to Cigarette Smoke

Open Journal of Pathology 2014年第3期4卷 125-137页

作者： Ana Maria Fleig-Mayer Alba Ramirez-Sarmiento Amanda Iglesias Montserrat Morla Carlos Coronell Diego Rodríguez Simona Pichinni Josep Lloreta Piergiorgio Zuccaro Xavier Busquets Joaquim Gea Jose R.Jardim Mauricio Orozco-Levi Centro Universitario do Triangulo(UNITRI) Avenida nicomedes Alves Dos Santos4545-GaveaUberlandiaPulmonary Rehabilitation CenterRespiratory DivisionFederal University of Sao PauloSao PauloBrazil Group of Research in injury Immune Response and Function(LIF)Municipal Institute of Medical Research(IMIM)Carrer Dr.Aiguader 88BarcelonaE-08003Spain Servei de Pneumologia Hospital del MarPasseig Maritim 25BarcelonaE-08003Spain Centro en Red de Investigacion de Enfermedades Respiratorias(CIBERES) Recinto Hospital Joan March Carretera Soller Km.1207110BunyolaMallorcaIlles BalearsSpain Institut Universitari d’Investigacio en Ciencies de la Salut(IUNICS)and Department de Biologia Universitat Illes Balears(UIB)Cra de ValldemossaKm 7.5PalmaIlles BalearsSpain Laboratorio Di Biochimica Clinica Istituto Superiore Di Sanita.Viale Regina Elena 29900161RomaItaly Respiratory Department Fundacion Cardiovascular de ColombiaCalle 155A 23-58FloridablancaSantanderColombia Servei d’Anatomia Patologica Hospital del MarPasseig Maritim 25BarcelonaE-08003Spain

Cigarette smoking is the main cause of chronic obstructive pulmonary disease (COPD). Diaphragm injury is observed in patients with COPD. However, the potential role of smoking in triggering or perpetuating muscle injury is unknown. The present study was aimed at evaluating the potential role of commercial tobacco smoke as a direct cause of skeletal muscle injury in experimental conditions. Seventy Wistar rats (170 - 250 g) were assigned to smoking (n = 49) or non-smoking (n = 21) groups. The smoking groups were submitted to a single or multiple (i.e., five or thirty) daily sessions of cigarette smoking in an inhalatory chamber (time length: 2 h each session). The level of exposure was constant and assessed by CO concentrations (50 ppm) and serum cotinine analysis. Animals submitted to a single smoke exposure and the corresponding controls were euthanized in groups at 0 h, 2 h, 4 h, 24 h or 48 h after completing the exposure. Animals submitted to multiple exposures were euthanized at 0 h after smoking. Samples from vastus lateralis muscle were obtained and processed for assessing cell injury and selected protein expression. Monoclonal anti-albumin antibodies were used to identify muscle fibers with sarcolemmal (membrane) injury. Subcellular muscle injury was assessed using transmission electron microscopy (EM). MyoD, myogenin and α-tubulin were immunodetected using western blot techniques. Exposure to cigarette smoke associated with significant membrane damage (mean relative difference (MRD) with controls: +181%, p = 0.004) and sarcomere disruptions (MRD: +226%, p = 0.001). Expression of MyoD and myogenin (normalized to α-tubulin) were significantly increased at 4 h and remained increased at 48 h post-exposure. We conclude that not only a single but also consecutive exposure to tobacco smoke have acute deleterious effects on peripheral muscle structure. A rapid induction of subrogate markers of skeletal muscle stress and repair processes associates to sarcolemmal and

关键词： Skeletal muscle muscle injury muscle Regeneration Quadriceps Cigarette Smoking

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The expression of basic fibroblastgrowth factor in processes of static load-induced skeletal muscle injury

The expression of basic fibroblastgrowth factor in processes...

引用

第29届FIMS世界运动医学大会

作者： Liu Ye, Luo Dongmei, Jin Jichun Sport Anatomy Section of Beijing Sport University, Beijing, 100084, China

Objective: To study the expression of basic fibroblastgrowth factor (bFGF) in this process by immunohistochemistry coloration and reverse transcriptase polymerasechain reaction technique. Methods: We designed a rat st... 详细信息

关键词： static load muscle injury bFGF expression

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Study of Mechanism on muscle injury Induced by Static Load

Study of Mechanism on Muscle Injury Induced by Static Load

引用

The6thPan-PacificConfere...

作者： Wang Sheng Limin Gao Lihua He Department of Occupational and Environmental Health Peking University Health Science Center Beijing CHINA

＜正＞In order to study the mechanisms of muscle injury induced by static load the intracellular Ca2 + concentrations, Ca2+-Mg2+-ATPase activity in mitochondria （MR） and sarcoplasmic reticulum（SR）, content of malondialdehyde（MDA）,activity of serum creatine kinase（CK） and alterations of energy supply were measured after static load. The results showed that muscular ATP content in treated groups decreased on the 4th day,6th day and 8th day respectively,and was significantly lower than that of controls on the 8th day（P＜0.05） .The content of PCr and glycogen showed a tendency of decreasing,but PCr was significantly lower than that of control group on the 6th day and 8th day （P ＜ 0.05, P ＜ 0. 01） while glycogen was significantly lower on the 8th day. The activity of serum CK was significantly higher than that of control group during the whole experiment period（P＜0. 01）. Intracellular Ca2+ concentration increased significantly on the 4th day（P＜0.05）. The content of Ca2+ in both SR and MR decreased but that of SR was significantly lower on the 6th day and 8th day（P＜0.01）. The activity of Ca2+ -Mg2+ -ATPase in MR and SR decreased significantly on the 6th day and 8th day（P＜0. 05）. The MDA content in MR increased on the 4th day while that of SR increased from the 6th day respectively, both showed significant difference from the controls（P＜0.01,P＜0. 05）. The results of those biochemical parameters demonstrated that in the process of static load the decrement of energy supply,alterations of intracellular Ca2+ *** enhancement of lipid hyperoxidation may play etiologic roles in muscle injury. However,relationships between those noxious factors remain to be further studied.

关键词： Static load muscle injury Ca2+ ion Ca2+ -Mg2+ -ATPase MDA

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Effects of estrogen on gastrocnemius muscle strain injury and regeneration in female rats

引用

Acta Pharmacologica Sinica 2004年第11期 105-110页

作者： Xin FENG, Guo-zhen LI, Sheng WANGDepartment of Occupational and Environmental Health, Public Health School, Peking University Health Science Center, Beijing 100083, China

AIM: To study the effects of estrogen on muscle damage and regeneration after acute passive gastrocnemius muscle strain injury in female Sprague-Dawley rats. METHODS: Rats were divided into 5 groups: ovariectomized, strained and treated with low-dosage estradiol (20 μg/d) (Elow), treated with high-dosage estradiol (200 μg/d) (Ehigh), treated with oil placebo (Oil), strained with no ovariectomy (Strain), and sham operated with no strain and no ovariectomy (Con). muscle damage index [plasma creatine kinase (CK)], antioxidant indexes [glutathione (GSH), Vitamin E (Vit E), total antioxidant capability (TAC)], and muscle regeneration index (desmin) were investigated at 7 d. RESULTS: The plasma CK activity increased but GSH, Vit E, and TAC levels decreased after muscle strain injury (Strain vs Con P<0.05). Plasma CK activity was the greatest while GSH, Vit E, and TAC were the lowest in the Oil group among the five groups (P<0.01). Plasma CK in the Ehigh and Strain groups was lower than that in the Elow group

关键词： estrogen skeletal muscle muscle injury antioxidant regeneration desmin

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Ultrasound guided acupotomy in treating rhomboid muscle and serratus posterior superior muscle injury:a case report

引用

TMR Non-Drug Therapy 2020年第3期3卷 125-130页

作者： Xiao-Jie Sun Yi-Feng Shen Yan Jia Zu-Yun Qiu Qiao-Yin Zhou Shi-Liang Li Department of Acupuncture and Moxibustion China-Japan Friendship HospitalNo.2East Yinghua RoadChaoyang DistrictBeijing 100029China Beijing University of Chinese Medicine No.11Bei San Huan Dong RoadChaoyang DistrictBeijing 100029China Hospital of Chengdu University of Traditional Chinese Medicine No.39Shi-er-qiao RoadChengdu 610072China Fujian University of Traditional Chinese Medicine Fuzhou 350122China

Objective:The study intended to show us the effect and safety associated with ultrasound-guidance during acupotomy treatment by a case ***:The research presented a case study using ultrasound-guided acupotomy in back *** study took place in the Department of Acupuncture and Moxibustion,China-Japan Friendship Hospital.A 56-year-old woman with paroxysmal right upper back pain for more than 10 years,who was diagnosed as the rhomboid muscle and serratus posterior superior muscle ***:After two acupotomy treatments,the Visual Analogue Score(VAS)of the patient declined from7 to *** month later,the pain disappeared and the patient was satisfied with ***:Ultrasound guidance can be used to observe the anatomical structures clearly to improve the safety of acupotomy treatment.

关键词： Upper back pain Acupotomy Ultrasound-guidance muscle injury

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Association between ACTN3 R577X genotype and risk of non-contact injury in trained athletes:A systematic review

引用

Journal of Sport and Health Science 2023年第3期12卷 359-368页

作者： Hassane Zouhal Juan Del Coso Ayyappan Jayavel Claire Toumy Guillaume Ravé Nidhal Jebabli Cain C.T.Clark Benjamin Barthélémy Anthony C.Hackney Abderraouf Ben Abderrahman M2S(Laboratoire Mouvement SportSanté)-EA 1274Department of Sport SciencesUniversity of RennesRennes F-35000France Rey Juan Carlos University Centre for Sport StudiesMadrid 28032Spain SRM College of Physiotherapy SRM Institute of Science and TechnologySRM NagarKattankulathur 603203India Department of Sport Sciences University of RouenMont Saint AignanCETAPS EA 3832F-76821France Toulouse Football Club Toulouse 31000France Higher Institute of Sport and Physical Education Ksar-SaidUniversity of ManoubaTunis 2010Tunisia Centre for Intelligent Healthcare Coventry UniversityCoventry CV15FBUK Department of Exercise&Sport Science Department of NutritionUniversity of North CarolinaChapel HillNC 27514USA

Purpose:The aim of this study was to review,systematically,evidence concerning the link between the ACTN3 R577X polymorphism and the rates and severity of non-contact injuries and exercise-induced muscle damage in athletes and individuals enrolled in exercise training ***:A computerized literature search was performed in the electronic databases PubMed,Web of Science,and SPORTDiscus,from inception until November *** included studies compared the epidemiological characteristics of non-contact injury between the different genotypes of the ACTN3 R577X ***:Our search identified 492 *** the screening of titles,abstracts,and full texts,13 studies examining the association between the ACTN3 genotypes and the rate and severity of non-contact injury were included in the *** studies were performed in 6 different countries(Spain,Japan,Brazil,China,the Republic of Korea,and Italy)and involved a total participant pool of 1093 *** the studies,2 studies involved only women,5 studies involved only men,and 6 studies involved both men and *** the studies included were classified as highquality studies(≥6 points in the Physiotherapy Evidence Database(PEDro)scale score).Overall,evidence suggests there is an association between the ACTN3 R577X genotype and non-contact injury in 12 *** studies observed a significant association between A CTN3 R577X polymorphism and exercise induced muscle damage:2 with non-contact ankle injury,3 with non-contact muscle injury,and 1 with overall non-contact ***:The present findings support the premise that possessing the ACTN3 XX genotype may predispose athletes to a higher probability of some non-contact injuries,such as muscle injury,ankle sprains,and higher levels of exercise-induced muscle damage.

关键词： α-actinin-3 deficiency Athletic performance Exercise-related injury muscle injury Single nucleotide polymorphism

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Magnetic Resonance Imaging and Histopathological Analysis of Experimental muscle Injuries in a Rabbit

引用

Biomedical and Environmental Sciences 2013年第10期26卷 841-848页

作者： YAN Dong ZHANG Jing LIANG Wei SUN Jing LIU Bao Yue TIAN Wei CHENG Xiao Guang Department of Radiology Beijing Jishuitan Hospital Fourth Clinical Hospital of Peking University Health Science Center

Objective To investigate the correlation of magnetic resonance imaging （MRI） with histopathologica changes, and to evaluate T2 mapping in assessing muscle trauma in a rabbit model of muscle injury. Methods We divided 35 rabbits into seven groups that each represented a different time point after intramuscular hemorrhage and muscle injury. Hemorrhage was created by injecting autologous blood into the left legs, and muscle injury was created by scalpel incision of the biceps femoris of the right legs. At different time points, the rabbits underwent Tl-weighted imaging and T2-weighted imaging （TzWl and T2Wl） and T2 mapping. T2 relaxation times were measured, and the corresponding samples were evaluated for pathological changes. Results After 2 h, the intramuscular hemorrhage model demonstrated an increased signal intensity on both TlWl and T2Wl. Histological examination showed erythrocytes within the muscle bundle. On days 1 and 3, the MRI signals were decreased, and there were no significant changes after day 7. From 2 h to 3 days, the muscle-injury model showed a high signal on both TzWl and T2Wl. Corresponding pathological changes included rupture and edema of muscle fibers, and inflammation. The abnormal signals were reduced on day 7. After day 14, the T2Wl intensity remained high. TlWl showed no abnormal changes, but some models showed a high signal, representing fresh bleeding and fatty tissue. T2 relaxation times were significantly different between the central and marginal regions, and between the marginal and normal regions. Conclusion MRI clearly demonstrates intramuscular hemorrhage and muscle injury, which correlate well with histopathological changes. Tz mapping is useful in assessing the extent of injury.

关键词： muscle injury Intramuscular hemorrhage Magnetic resonance imaging T2 mapping

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

A clinically relevant combination treatment with doxorubicin and cyclophosphamide does not induce hepatotoxicity in C57BL/6J mice

引用

Liver Research 2021年第4期5卷 239-242页

作者： Satyanarayana R Pondugula Julia M Salamat Kodye L Abbott Patrick C Flannery Mohammed Majrashi Mohammed Almaghrabi Manoj Govindarajulu Sindhu Ramesh Maninder Sandey Suneel K Onterue Chen-Che J Huang Yoshimi Iwaki Kristina Gill Natasha Narayanan Edwin McElroy Darshini Desai Rishi Nadar Timothy Moore Muralikrishnan Dhanasekaran Department of Anatomy Physiology and PharmacologyCollege of Veterinary MedicineAuburn UniversityAuburnALUSA Department of Pharmacology Faculty of MedicineUniversity of JeddahJeddahSaudi Arabia Department of Drug Discovery and Development Harrison School of PharmacyAuburn UniversityAuburnALUSA Department of Pathobiology College of Veterinary MedicineAuburn UniversityAuburnALUSA Animal Biochemistry Division National Dairy Research InstituteICAR-National Dairy Research InstituteKarnalHaryanaIndia Department of Clinical Science College of Veterinary MedicineAuburn UniversityAuburnALUSA

Background and aims:Chronic exposure to chemotherapeutics can lead to severe adverse events including hepatotoxicity.A combination chemotherapy regimen of doxorubicin(DOX)and cyclophos-phamide(CPS)is employed in treatment of several cancers such as leukemia,lymphoma,and breast *** is not well understood whether a combination therapy with DOX and CPS can induce *** therefore sought to determine whether co-administration of DOX and CPS at their clini-cally relevant doses and frequency results in ***:Male C57BL/6J mice received one intraperitoneal injection of saline or DOX-2 mg/kg and CPS-50 mg/kg once a week for 4 *** the treatment period,liver histology and various serum bio-markers of hepatotoxicity were ***:Co-treatment with DOX and CPS did not alter the serum levels of alanine aminotransferase(ALT),alkaline phosphatase(ALP),bilirubin,albumin,globulin,or total ***,co-administration of DOX and CPS did not result in a noticeable change in liver ***,it was notable that the concomitant treatment with DOX and CPS resulted in a significant increase in serum levels of aspartate aminotransferase(AST).Elevated serum AST levels were also associated with increased serum creatinine kinase(CK)levels,suggesting that the elevated serum AST levels are likely due to muscle injury following the co-administration of DOX and ***:Taken together,our results,for the first time,suggest that co-administration of DOX and CPS,at their clinically relevant doses and frequency does not induce a significant hepatotoxicity in the mice.

关键词： Cyclophosphamide(CPS) Doxorubicin(DOX) Hepatotoxicity Liver injury muscle injury

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Myofiber Permeability and Force Production of Rat muscles Following Eccentric Contractions: The Repeated Bout Effect Depends on the Interval

引用

Journal of Biomedical Science and Engineering 2020年第12期13卷 275-289页

作者： Keishi Hayao Hiroyuki Tamaki Keigo Tamakoshi Hideaki Takahashi Hideaki Onishi Institute for Human Movement and Medical Sciences Niigata University of Health and Welfare Niigata Japan Southern Tohoku General Hospital Fukushima Japan National Institute of Fitness and Sports in Kanoya Kagoshima Japan

Past eccentric contraction (ECC)-induced muscle injury reduces the severity of symptoms of subsequent muscle injury;this phenomenon is known as a repeated bout effect (RBE). It has been reported that increases in the duration of the interval between the first and second bouts are linked to weakening of the RBE. However, the histology following the attenuation of the RBE remains unclear. We examined the sustained effects of the second bout with regard to myofiber permeability and muscle force. Sixty-four male rats were randomly assigned to eight groups that varied in the number of exercise sessions and the duration of the interval between the first and second bouts: the non-ECC (Control);the single-injury (Post 1st bout);groups that were allowed to recover for 1, 2, and 4 weeks after a single injury (Pre 2nd bout_1w, Pre 2nd bout_2w, and Pre 2nd bout_4w);and groups that were subjected to second injuries 1, 2, and 4 weeks after the first (Post 2nd bout_1w, Post 2nd bout_2w, and Post 2nd bout_4w). The tibialis anterior was electrically stimulated in each ECC group. Twenty-four hours before muscle sampling, Evans blue dye (EBD) (a marker of myofiber damage) was administered. The maximal isometric contraction tension was measured immediately before sampling. The number of EBD-positive (+) fibers was determined via histological analysis. An RBE was revealed by functional examination at the 1- and 2-week and histological examination at the 1-, 2-, and 4-week time points (P < 0.05). In terms of myofiber permeability, prolongation of the interval before the second bout weakened this effect (P < 0.05). Experiments with 1-, 2-, and 4-week intervals indicated that prolongation of the interval before the second bout weakened the RBE with regard to myofiber permeability.

关键词： Eccentric Contraction muscle injury Repeated Bout Effect Myofiber Membrane Permeability Maximal Isometric Contraction Tension

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：