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Comparison of the characteristics of macrophages derived from murine spleen, peritoneal cavity, and bone marrow

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2017年第12期18卷 1055-1063页

作者： Yan-long ZHAO Pu-xun TIAN Feng HAN Jin ZHENG Xin-xin XIA Wu-jun XUE Xiao-ming DING Chen-guang DING Department of Kidney Transplantation Hospital of Nephropathythe First Affiliated Hospital of Xi'an Jiaotong UniversityXi'an 710061China Institute of Organ Transplantation Xi'an Jiaotong UniversityXi'an 710061China Department of Traditional Chinese Medicine the First Affiliated Hospital of Xi'an Jiaotong UniversityXi'an 710061China

Macrophages have a diverse set of functions based upon their activation states. The activation states, including resting（M0） and polarizing（M1 and M2） states, of macrophages derived from the mouse bone marrow, spleen, and peritoneal cavity（BMs, SPMs, and PCMs, respectively） were compared. We evaluated the macrophage yield per mouse and compared the surface markers major histocompatibility complex（MHC） Ⅱ and CD86 by flow cytometry. The relative mRNA levels of tumor necrosis factor-α（TNF-α）, interleukin（IL）-1β, mannose receptor（MR）, and Ym1 in the M0, M1, and M2 states were also compared using real-time polymerase chain reaction（PCR） analysis. Bone marrow yielded the most macrophages with the best homogeneity, but they were polarized toward the M2 phenotype. All three types of macrophages had the capacity to polarize into the M1 and M2 states, but SPMs had a stronger capacity to polarize into M1. The three types of macrophages showed no differences in their capacity to polarize into the M2 state. Therefore, the three types of macrophages have distinct characteristics regardless of their resting or polarizing states. Although bone marrow can get large amounts of homogeneous macrophages, the macrophages cannot replace tissue-derived macrophages.

关键词： Macrophage murine splenocytes murine bone marrow Peritoneal cavity

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