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Improvement of the tissue culture technique for Melaleuca alternifolia

Journal of Forestry Research 2016年第6期27卷 1265-1269页

作者： Bihua Chen Jianmin Li Juan Zhang Huihua Fan Lijun Wu Qianzhen Li Fujian Academy of Forestry Sciences Fuzhou 350012People's Republic of China Key Laboratory of Timber Forest Breeding and Cultivationfor Mountainous Areas in Southern China China ForestryBureauFuzhou 350012People's Republic of China

Tea tree oil is extracted from the leaves and twigs of Melaleuca alternifolia （Maiden ＆ Betche） Cheel, and it is widely used in medicines, food preservatives, cosmetics and health care products. Traditional propagation of M. alternifolia from seeds does not necessarily transfer the desired characteristics from their mother trees, the seedlings are not uniform, and the multiplication rate from cuttings is relatively low. For these reasons, it is necessary to develop tissue culture techniques for this species. This study showed that an efficient explant initiation medium for M. alternifolia was MS 1/2 ＋ BA 0.6mg L^-1 ＋NAA 0.1 mg L^-1＋sucrose 30g L-l, which yielded a 75.9 % initiation rate. An efficient multi- plication medium was MS ＋ BA 0.3 mg L^-1＋ NAA 0.15 mg L^-1 ＋ sucrose 30 g L^-1, which yielded a 4.3 multiplication rate and 3.2 cm shoot length. The rooting medium was MS 1/2 ＋ IBA 0.1-0.25 mg L^-1 ＋ sucrose 15 g L^-1, which yielded a 100 % rooting rate, 2.94-3.32 roots per individual and 1.36-1.44 cm root length. Local red-core soil was suitable as a transplant medium, and yielded 98 % survival. This study improved the tissue culture technique for mass-propagation of M. alternifolia, enabling the production of high quality plants for market.

关键词： Melaleuca alternifolia Tissue culture multiplication rate Rooting Survival rate

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Biocontrol potential of entomopathogenic nematode,Heterorhabditis indica against pink bollworm,Pectinophora gossypiella(Saunders)(Lepidoptera:Gelechiidae)

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Journal of Cotton Research 2023年第4期6卷 277-287页

作者： THUBE Shivaji SHINDE Swati SHAH Vivek GOKTE‑NARKHEDKAR Nandini INGOLE Dnyaneshwar NIKOSHE Akash TENGURI Prabhulinga THAVKAR Snehal FAND Babasaheb DESHMUKH Vrushali PRASAD Yenumula Indian Council of Agricultural Research-Central Institute for Cotton Research NagpurMaharashtra 440010India College of Agriculture NagpurMaharashtra 440001India Dr.Balasaheb Sawant Konkan Krishi Vidyapeeth DapoliMaharashtra 415712India

Background The emergence of pink bollworm(PBW),Pectinophora gossypiella(Saunders)(Lepidoptera:Gelechiidae),in cotton due to Bt resistance and concealed feeding habit has created a need for alternative,eco-friendly,and cost-effective control *** study aimed to evaluate the bio-efficacy and reproductive potential of two native strains of entomopathogenic nematodes(EPNs),Heterorhabditis indica,namely CICR-HI-CL and CICR-HI-MN,against PBW larvae and pupae under in-vitro *** The larval assay revealed that strain CICR-HI-CL exhibited higher potency than strain CICR-HI-MN against 2nd,3rd,and 4thinstar larvae,with median lethal dose(LD50)values of 5.45,4.45,and 4.60 infective juveniles(IJs)per larva,*** case of pupal bioassay,both EPN strains demonstrated greater virulence when applied directly(LD50values:29.65 and 73.88 IJs per pupa for strains CICR-HI-CL and CICR-HI-MN,respectively)compared to soil application(147.84 and 272.38 IJs per pupa).Both EPN strains successfully penetrated and reproduced on 4thinstar larvae,resulting in maximum production of 19.28 and 20.85 lakh IJs per larva in the next generation when inoculated at 30 IJs per *** The present study has generated useful information on the virulence and reproductive potential of two strains of EPN ***(CICR-HI-CL and CICR-HI-MN)against PBW,a dreaded pest of *** virulence and reproductive potential of EPN strains demonstrated their ability to multiply,sustain and perpetuate on larval and pupal stages of *** knowledge generated will help formulate effective management strategies for PBW with the inclusion of EPN as a potential biological control *** soil-dwelling life stages viz.,last instar hibernating larvae and pupae of PBW can be the ideal weak links to make a successful use of *** for sustainable management of PBW in the cotton ***,before taking these EPN strains to field for managing PBW,detailed studies investigati

关键词： Bio-efficacy Entomopathogenic nematodes multiplication rate Heterorhabditis indica Pectinophora gossypiella

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Can Kponan Yam （Dioscorea cayenensis） Full Season Tuber Sprout If Planted？

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Journal of Agricultural Science and Technology(B) 2014年第4期4卷 312-320页

作者： Soro Dognimeton Ayolie Koutoua Bakayoko Sidiky Dao Daouda Yatty Kouadio Justin Gnakri Dago Department of Agroforestery University Jean Lorougnon Guede Daloa BP 150 Cote d Tvoire Swiss Centre for Scientific Research Abidjan 01 Cote d'lvoire

In a completely randomized blocks （RCB） plot, we planted seed from the three segments of Kponan tubers： head, median and tail portions with control Krengle. Germination began 10 d and lasted 67 d. The mean sprouting rate of the Kponan full season harvest tuber was 78.1% compared to 94% in Krengle. However, the tuber germination was progressive from head to tail with a linear evolution of the top fragments with sprouting rate up to 90% similar to Krengle. The median and distal portions sprout two and three weeks later with final raising rates reaching 68% and 71%, respectively. The full season tuber of Kponan sprouts with a good rate gave good yields for any portion of the tuber. It had a sprouting gradient oriented from the proximal portion to the distal one, while yield was better for medium seeds. The sprouting delays two weeks between proximal and medium portions and three weeks between proximal and distal portions were acceptable with regards to the total cycle of eight to nine months period. Thus, its small tubers could be planted as well as the top portion of big tuber. In terms of yield, Kponan medium seed gave the best yield 34.25 t/ha and the whole Kponan tuber yield was more than Krengle. That led to a better multiplication factor for Kponan full season tuber. The type of tuber, if available, could be used as seed for yam production. That could improve its distribution and contribute to food security.

关键词： Yam full season harvest sprout tuber yield multiplication rate C6te d'Ivoire.

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