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The testis:an accessible Mesenchymal Stem Cells source

Advances in Modern Oncology Research 2018年第6期4卷 5-17页

作者： Myret Ghabriel Asma Amleh Biotechnology Program American University in CairoAUC AvenueP.O.Box74New Cairo 11835Egypt Department of Biology American University in CairoEgypt

Mesenchymal stem cells(MSCs)are adult stem cells that are predominantly found in bone marrow but also cord blood,peripheral blood,lungs,and adipose *** are considered multipotent cells,which can differentiate to form multiple cells types—adipocytes,bone,cartilage,skin,and *** potential elicits a great deal of attention because it appears to hold great promise regarding regenerative cell-based therapy to treat various diseases,including *** are considered powerful allogeneic nonhematopoietic transplantation stem cells due to their low ***,several researchers have identified and isolated MSCs from primary human testes *** review aimed to highlight the latest advances in MSC biological properties and characteristics studies,focusing on testicular mesenchymal stromal stem cells(tMSC),their differentiation capacity,and immunosuppressive properties—all of which have the potential to establish MSCs as powerful therapeutic agents for various diseases,including cancer.

关键词： Testicular mesenchymal stromal stem cells MSC sources MSC isolation multilineage potential therapeutic application

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Characterization of mouse clonal mesenchymal stem cell lines established by subfractionation culturing method

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World Journal of Stem Cells 2011年第8期3卷 70-82页

作者： Myung-Shin Jeon Tac-Ghee Yi Hyun-Ja Lim Sun-Hwa Moon Moon-Hee Lee Joon-Soon Kang Chul-Soo Kim Dae-Hyun Lee Sun U Song Clinical Research Center School of Medicine Inha University 7-2063-GaShinheung-DongChung-GuInchon 400-711South Korea

AIM:To characterize single-cell-derived mouse clonal mesenchymal stem cells (mcMSCs) established with bone marrow samples from three different mouse strains. METHODS:We established mcMSC lines using subfractionation culturing method from bone marrow samples obtained from long *** lines were characterized by measuring cell growth, cell surface epitopes, differentiation potential, lineage-specific gene expression and T-cell suppression capability. Nonclonal MSCs isolated by the conventional gradient centrifugation method were used as controls. RESULTS:All mcMSC lines showed typical nonclonal MSC-like spindle shape morphology. Lines differed inoptimal growth density *** surface epitope prof iles of these mcMSC lines were similar to those of nonclonal MSCs. However, some lines exhibited different expression levels in a few epitopes, such as CD44 and CD105. Differentiation assays showed that 90% of the mcMSC lines were capable of differentiating into adipogenic and/or chondrogenic lineages, but only 20% showed osteogenic lineage differentiation. T-cell suppression analysis showed that 75% of the lines exhibited T-cell suppression capability. CONCLUSION:mcMSC lines have similar cell morphology and cell growth rate but exhibit variations in their cell surface epitopes, differentiation potential, lineage-specifi c gene expression and T-cell suppression capability.

关键词： Clonal stem cells Bone marrow stem cells multilineage differentiation Clonal stromal cell isolation T-cell suppression

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