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Food‑grade expression of multicopper oxidase with improved capability in degrading biogenic amines

Systems Microbiology and Biomanufacturing 2022年第2期2卷 285-295页

作者： Xiumei Ni Jian Chen Guocheng Du Fang Fang Key Laboratory of Industrial Biotechnology Ministry of EducationSchool of BiotechnologyJiangnan UniversityWuxi 214122JiangsuChina Science Center for Future Foods Jiangnan UniversityWuxi 214122JiangsuChina Engineering Research Center of Ministry of Education on Food Synthetic Biotechnology Jiangnan UniversityWuxi 214122JiangsuChina Jiangsu Province Engineering Research Center of Food Synthetic Biotechnology Jiangnan UniversityWuxi 214122JiangsuChina National Engineering Laboratory for Cereal Fermentation Technology Jiangnan UniversityWuxi 214122JiangsuChina The Key Laboratory of Carbohydrate Chemistry and Biotechnology Ministry of EducationJiangnan UniversityWuxi 214122JiangsuChina

Biogenic amines(BAs)are potential amine hazards that are detected in fermented foods and alcoholic *** intake of BAs may lead to allergic symptoms such as difculty in breathing,nausea,and *** of BAs by multicopper oxidase(MCO)is a promising method as it has little efect on the fermentation process,food nutrition,and ***,the application of MCO in food industry was restricted due to its poor catalytic properties and low *** this work,food-grade expression of the Bacillus amyloliquefaciens MCO(MCOB)and its three mutants were successfully constructed in Lactococcus lactis *** expression level of MCOB in *** NZ3900 was dramatically enhanced by optimizing the cultivation conditions,and the highest expression level reached 4488.1 U/*** was the highest expression level of food-graded MCO reported so far,to our ***,the optimal reaction pH of MCOB expressed in *** NZ3900 switched to 4.5,it would be more suitable for degrading BAs in food as the pH value of most fermented foods was found to be ***,MCOB expressed in *** NZ3900 was quite stable(with more than 80%residual activity)in the pH range of 4.0–5.5,the catalytic rate constant(kcat)and specifc activity of MCOBLS were all dramatically increased compared with that of MCOB expressed in Escherichia *** histamine as the substrate,the degradation of BAs within 24 h by MCOB expressed in *** NZ3900 was 69.7%higher than that expressed in *** results demonstrated the potential applications of MCOB in food industry for reduction of biogenic amines.

关键词： Biogenic amines Histamine Degradation multicopper oxidase Food-grade expression

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Laccases from Actinobacteria—What We Have and What to Expect

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Advances in Microbiology 2014年第6期4卷 285-296页

作者： Tatiana Alves Rigamonte Fernandes Wendel Batista da Silveira Flávia Maria Lopes Passos Tiago Domingues Zucchi Embrapa Meio Ambiente Rodovia SP 340JaguariúnaBrazil Departamento de Microbiologia BIOAGROUniversidade Federal de ViçosaCampus UniversitarioVicosaBrazil

Laccases are blue multicopper enzymes, capable of oxidizing diverse aromatic and non-aromatic compounds of industrial interest, concomitantly with reduction of molecular oxygen to water. Tolerance to extreme conditions, such as high temperature, salinity or extreme pH, is required for practical industrial applications. Here we focus on bacterial laccases from the phylum Actinobacteria, notably the order Actinomycetales. Currently, less than 10 enzymes have been properly characterized, all belonging to genus Streptomyces, but it is noteworthy that all of them have exhibited industrially important properties. Furthermore, studies with enzymes from this phylum revealed a novel molecular structure of laccases, providing the basis for a distinct family, the two-domain laccases. The relevant traits of actinomycetes laccases emphasize the need for more studies involving the isolation of this bacterial group from lignin-rich environmental samples, detection of their laccase activity and thereafter, characterization of the proteins and related genes. The nonhomogeneous responses of actinomycetes laccases to traditional inhibitors, substrates or metal ions have challenged the currently accepted “laccase concept”. Finally, considering that distinguishing laccase activity in vitro from other ligninolytic enzymes becomes a difficult task due to overlaps in catalytical properties of the enzymes, we proposed a simple flow chart to help experimental assays.

关键词： Actinomycetes Lignin Degradation multicopper oxidase Laccases

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