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Positional assembly of multi-enzyme cascade reaction in polyelectrolyte doped microcapsule through electrospray and layer-by-layer assembly

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Synthetic and Systems Biotechnology 2020年第3期5卷 206-213页

作者： Shiyi Che Jie Wang Xiaoyuan Ji Zhiguo Su Shaomin Wang Songping Zhang State Key Laboratory of Biochemical Engineering Institute of Process EngineeringChinese Academy of SciencesBeijing100190China University of Chinese Academy of Sciences No.19 Yuquan RoadShijingshan DistrictBeijing100049China College of Chemistry Zhengzhou UniversityZhengzhou450001China

Polyelectrolyte-doped microcapsules(PDM)was fabricated by coaxial electrospray of a mixture of glycerol and water containing 10 mg/mL cationic polyelectrolyte poly(allylamine hydrochloride)(PAH)fed as the core phase solution,and a N,N-dimethylacetylamide solution of 10 wt%polyurethane fed as the shell phase *** system involving Candida Antarctica lipase B(CALB),glucose oxidase(GOD),and horseradish peroxidase(HRP)for cascade reaction was assembled in the PDM at three different places,namely,surface,shell,and *** of enzyme inside aqueous lumen of the PDM was realized by in situ encapsulation through adding the enzyme in the core-phase solution for coaxial *** ion-pairing of enzyme with cationic surfactant CTAB,an organic soluble enzyme-CTAB complex was prepared,so that in situ embedding of enzyme in the shell of the PDM was realized by adding it into the shell phase *** attachment of enzymes was achieved by layer-by-layer(LbL)technology,which is based on the ion-exchange interactions between oppositely charged enzymes and PAH that was doped in *** enzyme-decorated microcapsule was then studied as a microbioreactor,in which 1-Oxododecyla-α-glucopyranoside was converted by CALB to glucose,which was oxidised by GOD to gluconolactone in a second *** hydrogen peroxide produced was then used by HRP to oxidize ABTS to form coloured radical cation ABTS•+for activity *** successful fabrication of the PDM and precise localization of enzymes in the PDM by different strategies were fully *** varying the immobilization strategy,totally six PDM bioreactors with three enzymes precisely positional assembled in different strategies were constructed and their activities for the cascade reaction were investigated and *** PDM micro-bioreactor prepared by novel electrospray technologies provide a smart platform for positional assembly of multi-enzyme cascade reaction in a precise and well-controlle

关键词： Coaxial electrospray Microcapsule multi-enzyme Cascade reaction Positional assembly Layer-by-layer assembly

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Cover Story

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Chinese Chemical Letters 2019年第5期30卷 F0003-F0003页

作者： Quan Yuan 不详

Construction of multi-enzyme systems not only can help expand the understanding of physiological and pathological mechanisms,but also holds great promise in biosynthesis,biosensing and ***,lots of strategies have been exploited to arrange multiple enzymes into a confined space for the construction of artificial multi-enzyme complexes,including liposomes encapsulating,polymersomes entrapping,chemical crosslinking and covalent *** these strategies are promising,there are still several obstacles remaining which may impede the practical *** example,synthesis of these multi-enzyme complexes involves tedious preparations and multiple steps,leading to the loss of enzyme activity and further decreasing the catalytic ***,a simple and efficient strategy to develop multi-enzyme complexes remains a great challenge.

关键词： Cover Story Construction multi-enzyme systems

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