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Evaluation of antioxidant capacity and reproductive toxicity of aqueous extract of Thai mucuna pruriens seeds

Journal of Integrative Medicine 2020年第3期18卷 265-273页

作者： Sitthichai Iamsaard Supatcharee Arun Jaturon Burawat Supataechasit Yannasithinon Saranya Tongpan Sudtida Bunsueb Natthapol Lapyuneyong Pannawat Choowong-in Nareelak Tangsrisakda Chadaporn Chaimontri Wannisa Sukhorum Department of Anatomy Faculty of MedicineKhon Kaen UniversityKhon Kaen 40002Thailand Research Institute for Human High Performance and Health Promotion Khon Kaen UniversityKhon Kaen 40002Thailand Faculty of Nursing Ratchathani UniversityUbon Ratchathani 34000Thailand School of Medicine Mae Fah Luang UniversityChiang Rai 57100Thailand

Objective: In traditional medicine, the seeds of Thai mucuna pruriens(T-MP) are used to treat male dysuria and are believed to enhance fertility. However, information pertaining to the toxicity of T-MP and its interaction with other properties is limited. This study was thus conducted to evaluate the antioxidant capacity and subacute toxicity of T-MP in the reproductive system.Methods: Total phenolic content and antioxidant capacity of T-MP seed extract were determined using total phenolic content, 2,2-diphenyl-1-picrylhydrazyl and ferric reducing antioxidant power assays.Male and female adult rats were treated orally with T-MP at a dosage of 150 or 300 mg/kg body weight for 14 consecutive days. Sex hormones and functional parameters in the liver and kidney were evaluated.Histopathology of all tissue was conducted using Masson’s trichrome staining. Sperm parameters, including concentration, morphology, acrosome reaction status and DNA damage, were also examined.Expression of tyrosine phosphorylated protein(Tyr Pho), androgen receptor and A-kinase-anchoring protein 4(AKAP4) were investigated using the Western blot technique.Results: T-MP seed extract contained phenolic compounds and exhibited high antioxidant capacity with no toxicity at the tested doses. It did not affect liver or kidney function parameters in the male rats, but increased estradiol, aspartate aminotransferase and alanine aminotransferase levels in the females.Additionally, it decreased serum progesterone and alkaline phosphatase levels in female rats. Serum and intratesticular testosterone levels were significantly lower in male rats that received a high dosage of T-MP. Histopathological changes were not observed in any tissue treated with T-MP. T-MP also significantly increased sperm concentration(but did not affect sperm parameters), and enhanced testicular Tyr Pho protein and androgen receptor and expression of AKAP4 in sperms.Conclusion: T-MP seed extract exhibited antioxidant capacity and was not harmful to reproductive tissues. It also had

关键词： Mamui mucuna pruriens Reproductive system Subacute toxicity Rats

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Evaluation of the anti-diabetic properties of mucuna pruriens seed extract

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Asian Pacific Journal of Tropical Medicine 2011年第8期4卷 632-636页

作者： Stephen O Majekodunmi Ademola A Oyagbemi Solomon Umukoro Oluwatoyin A Odeku Department of Pharmaceutics and Pharmaceutical Technology Faculty of PharmacyUniversity of UyoUyoNigeria Department of Pharmaceutics and Industrial Pharmacy Faculty of PharmacyUniversity of IbadanIbadanNigeria Department of Veterinary Physiology Biochemistry & PharmacologyUniversity of IbadanNigeria Department of Pharmacology and Therapeutics College of MedicineUniversity of IbadanIbadanNigeria

Objective:To explore the antidiabetic properties of mucuna pruriens(M.pruriens).Methods: Diabetes was induced in Wistar rats by single intravenous injection of 120 mg/kg of alloxan monohydrate and different doses of the extract were administered to diabetic rats.The blood glucose level was determined using a glucometer and results were compared with normal and untreated diabetic rats.The acute toxicity was also determined in albino mice.Results:Results showed that the administration of 5,10,20,30,40,50,and 100 mg/kg of the crude ethanolic extract of M.pruriens seeds to alloxan-induced diabetic rats(plasma glucose > 450 mg/dL) resulted in 18.6%,24.9%,30.8%,41.4%,49.7%,53.1%and 55.4%reduction,respectively in blood glucose level of the diabetic rats after 8h of treatment while the administration of glibenclamide (5 mg/kg/day) resulted in 59.7%reduction.Chronic administration of the extract resulted in a significant dose dependent reduction in the blood glucose level(P<0.001).It also showed that the antidiabetic activity of M.pruriens seeds resides in the methanolic and ethanolic fractions of the extract.Acute toxicity studies indicated that the extract was relatively safe at low doses,although some adverse reactions were observed at higher doses(8-32 mg/kg body weight),no death was recorded.Furthermore,oral administration of M.pruriens seed extract also significandy reduced the weight loss associated with diabetes.Conclusions:The study clearly supports the traditional use of M.pruriens for the treatment of diabetes and indicates that the plant could be a good source of potent antidiabetic drug.

关键词： Antidiabetic mucuna pruriens Hypoglycemic activity Toxicity Diabetes mellitus

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The extract of mucuna pruriens possesses histamine activity

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Asian Pacific Journal of Tropical Medicine 2010年第1期3卷 29-31页

作者： Reginald NP Nwankwoala Georgewill OA Department of Pharmacology and Toxicology Faculty of Basic Medical SciencesUniversity of Port Harcourt

Objective:To investigate the activity of the active principle in the spines of the seed pods of mucuna pruriens using contraction of guinea pig ileum as index of pharmacological activity. Methods:The active principle was extracted with 0.0015 M NaCl.Muscle strips of guinea pig ileum were prepared and contractile responses were measured using a Kymograph.Two sets of experiment were conducted:（1）.The contraction of the ileum in presence of different concentrations of histamine,2-methylhistamine and the extract of mucuna pruriens.（2）.The contractile response of the ileum in presence of different concentrations of the extract and antagonists including diphenhydramine,atropine and methysergide.Results:（1） The extract of mucuna pruriens hair, 2- methylhistamine and histamine produced dose dependent contraction of guinea pig ileum (Extract ED50 = 13.0μg/mL,2-methylhistamine ED50=8.5μg/mL and histamine ED50=10.0μg/ mL).（2） Diphenhydramine,an H,antagonist competitively blocked the contractile response of the mucuna pruriens extract（3） Coadminstration of the mucuna pruriens extract either with different doses of antimuscarinic agent atropine or 5-hydroxytryptamine blocking agent methysergide did not alter the extract induced contractile response of the guinea pig ileum.Conclusion:These results demonstrate that the spines of mucuna pruriens possess histamine activity which may contribute to its itching and painful irritation effects.

关键词： mucuna pruriens Histamine activity Itching Irritation

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Effect of aqueous seed extract of mucuna pruriens on arsenic-induced testicular toxicity in mice

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Asian pacific Journal of Reproduction 2020年第2期9卷 77-82页

作者： Preethi Concessao Laxminarayana Kurady Bairy Archana Parampalli Raghavendra Department of Physiology Melaka Manipal Medical CollegeManipal Academy of Higher EducationManipalKarnataka576104India Department of Pharmacology RAK College of Medical SciencesRAK Medical and Health Sciences UniversityRas Al KhaimahUnited Arab Emirates

Objective:To investigate the protective effects of mucuna(M.)pruriens against arsenic-induced testicular impairment in albino mice.Methods:Thirty-six male albino mice were divided into six groups,with 6 mice in each group.Group 1 received drinking water as the normal control.Groups 2 to 6 received sodium arsenite(40 mg/L)in drinking water.Group 3 received sodium arsenite(40 mg/L)and 300 mg/kg body weight(b.w.)of N-acetylcysteine.Group 4 received sodium arsenite(40 mg/L)and 500 mg/kg b.***.of M.pruriens.Group 5 received sodium arsenite(40 mg/L)and 1000 mg/kg b.***.of M.pruriens.Group 6 received sodium arsenite(40 mg/L)and 2000 mg/kg b.***.of M.pruriens.N-acetylcysteine and M.pruriens were administered orally once a day.Animals were subjected to the above treatments for 45 days.Animals were sacrificed with overdose of ketamine 24 h following drug administration.The testis was used for biochemical estimations(lipid peroxidation and glutathione),and the epididymis was used to determine the sperm count and morphology.Results:Sodium arsenite significantly decreased(P<0.01)the sperm count and glutathione levels of the testis.It significantly increased(P<0.01)the abnormal architecture of the spermatozoa and lipid peroxidation levels.Treatment with M.pruriens significantly increased the sperm count and the glutathione levels[500 mg/kg b.***.and 1000 mg/kg b.w.(P<0.01)].A significant decrease in sperm abnormality and lipid peroxidation levels[500 mg/kg b.***.and 1000 mg/kg b.w.(P<0.01)]was also observed.Conclusions:The perturbed sperm parameters and antioxidant levels of the arsenic insulted testis are attenuated by 500 mg/kg b.***.and 1000 mg/kg b.w.M.pruriens.

关键词： Sodium arsenite mucuna pruriens Sperm morphology Sperm count Testis

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Phenolic composition and inhibitory activity of Mangifera indica and mucuna urens seeds extracts against key enzymes linked to the pathology and complications of type 2 diabetes

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Asian Pacific Journal of Tropical Biomedicine 2014年第11期4卷 903-910页

作者： Emmanuel Anyachukwu Irondi Ganiyu Oboh Afolabi Akintunde Akindahunsi Aline Augusti Boligon Margareth Linde Athayde Department of Biochemistry Federal University of Technology.P.M.B.704Akure 340001Nigeria Biochemistry Unit Department of Biosciences and BiotechnologyKwara State UniversityMaleteP.M.B.1530IlorinNigeria Phytochemical Research Laboratory Department of Industrial PharmacyFederal University of Santa MariaBuilding 26.Room 1115.Santa Maria.CEP 97105-900.Brazil

Objective:To investigate the phenolic compounds composition and the inhibitory activity of Mangifera indica(M.indica) and mucuna urens(M.urens) seeds extracts against some key enzymes(-amylase,-glucosidasc and aldose reductase) implicated in the pathology and complications of type 2 diabetes in vitro.Methods:Reverse phase chromatographic quantification of the major flavonoids and phenolic acids in the seeds extracts was carried out using high performance liquid chromatography coupled with diode array detection.The inhibitory activities of the seeds extracts against-amylase and-glucosidase were estimated using soluble starch and p-nitrophenylglucopyranosidc as their respective substrates.Inhibition of aldose reductase activity by the extracts was assayed using partially purified lens homogenate of normal male rat as source of enzyme;inhibition of Fe^(2+)-induced lipid peroxidation by extracts was tested in rat pancreas homogenate.Results:The chromatography result revealed that extracts of both seeds had appreciable levels of some major flavonoids and phenolic acids of pharmacological importance,including gallic acid,chlorogenic acid,caffeic acid,ellagic acid,catechin,rutin,quercitrin.quercetin and kaempferol.Extracts of both seeds effectively inhibited-amylase,-glucosidase and aldose reductase activities in a dose-dependent manner,having inhibitory preference for these enzymes in the order of aldose reductase>-glucosidase>-amylase.With lower half-maximal inhibitory concentrations(IC50)against-amylase,-glucosidase,and aldose reductase,M.indica had stronger inhibitory potency against these enzymes than M.urens.Extracts of both seeds also inhibited Fe2+-induced lipid peroxidation in a dose-dependent pattern,with M.indica being more potent than M.urens.Conclusions:The results obtained provide support for a possible use of M.indica and M.urens seeds in managing hyperglycemia and preventing the complications associated with it in type 2diabetes.

关键词： Hyperglycemia Diabetic complications Enzyme inhibition Lipid peroxidation Mangifera indica mucuna urens

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Phytoremediation of Soil Contaminated with Crude Oil Using mucuna Bracteata

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Research in Ecology 2019年第1期1卷 20-30页

作者： Kuok Ho Daniel, Tang Yu Wei Eric, Law Department of Environmental Engineering Curtin University MalaysiaCDT25098009 MiriSarawakMalaysia

This study examines the ability of mucuna bracteata DC. to remediate soil contaminated with increasing levels of crude oil up to 20%. It also investigates the effect of fertilizer application on crude oil degradation. Changes in crude oil concentrations, pH and moisture of the soil in eight experimental pots were tracked over a period of 9 weeks. The crude oil levels in soil were analysed as Total Petroleum Hydrocarbons (TPHs) using the UV-Vis spectrophotometer. The study revealed the capacity of mucuna bracteata to phytoremediate soil contaminated with crude oil in all experimental pots though the plant died at 20% contamination towards the end of the experiment. The plant survived up to 15% contamination with that in the fertilized pot showing better physiological conditions. In all instances, fertilized pots showed higher rates of crude oil reduction. The amounts of crude oil degraded in fertilized pots were also higher except at 20% contamination. The soil pH varied over a narrow range throughout the experimental period. Moisture of soil contaminated with 15% and 20% crude oil was higher than that contaminated with 5% and 10% crude oil. mucuna bracteata showed signs of phytoextraction which can be subject to further study. This study contributed a new candidate of phytoremediation for soil contaminated with high level of crude oil.

关键词： mucuna bracteata Rhizodegradation Contamination Fertilizer Total Petroleum Hydrocarbon Phytoextraction

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Physicochemical and Functional Characterization of mucuna pruriesDepigmented Starch for Potential Industrial Applications

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International Journal of Organic Chemistry 2015年第1期5卷 1-10页

作者： Maira Rubi Segura-Campos Sonia Marina López-Sánchez Arturo Castellanos-Ruelas David Betancur-Ancona Luis Chel-Guerrero Facultad de Ingeniería Química Universidad Autónoma de Yucatán Mérida México

Starch is a very important biopolymer in the food industry. The velvet bean (M. pruriens) is an excellent potential starch source containing approximately 520 g starch per kg. The objective of this study was to evaluate the physicochemical and functional properties of velvet bean depigmented starch. The starch granules appear oval and spherical shaped. The colour registered L*, a*, b* values of 44.9, 0.324 and 0.341 respectively. The chemical composition registered values of moisture, ash, fat, protein, fibre and NFE of 110.5, 5.8, 5.7, 0.0, 34 and 954.5 g/kg respectively, as well as amylose levels of 215.3 g/kg. Gelatinization onset (To), peak (Tp) and final (Tf) temperatures were of 74.23°C, 80.57°C and 86.39°C. The solubility (3.1% - 16.2%), swelling power (SP) (2.86% - 16.17%) and water absorption capacity (WAC) (2.67 - 15.95 g water/g starch) were directly correlated to temperature (60°C - 90°C). The enthalpy values (4.10 - 13.47 j/g) were directly correlated to the time (1 - 21 days). The retrogradation increased as time increased. The viscosity of M. pruriens depigmented starch decreased slightly during the heating stages and then increased during cooling and the refrigeration and freezing stability registered syneresis ranges from 17.65 to 23.18 mL/50mL and from 16.4 to 22.6 mL/50mL respectively, indicating that the depigmented starch was unstable in heating-cooling processes.

关键词： Depigmented Starch Functional Properties mucuna pruriens Physicochemical Properties

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ACE-I inhibitory peptide fractions from enzymatic hydrolysates of velvet bean (mucuna pruriens)

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Agricultural Sciences 2013年第12期4卷 767-773页

作者： Maira Rubi Segura-Campos Carlos Paul Espadas-Alcocer Luis Chel-Guerrero David Betancur-Ancona Facultad de Ingeniería Química Universidad Autónoma de Yucatán Mérida México

The hydrolysis of velvet bean (mucuna pruriens) protein in the presence of Alcalase?-Flavourzyme? and Pepsin-Pancreatin was investigated. The results showed that Alcalase?-Flavourzyme? (29.08%) sequential system catalyzed the hydrolysis most efficiently that Pepsin-Pancreatin (24.78%). In addition, the higher ACE-I inhibitory activity was achieved with the sequential system Alcalase?-Flavourzyme? (33.13%). Furthermore, the concentration of peptides employing an ultrafiltration (UF) system or their purification by gel filtration chromatography showed that the oligomeric peptides with lower molecular weight registered the highest ACE-I inhibitory activity. It has been demonstrated that mucuna pruriens protein hydrolysates could serve as a source of peptides with ACE inhibitory activity and this activity can be attributed mainly to the mixture of short peptides in the hydrolysate.

关键词： Velvet Bean mucuna pruriens ACE-I Inhibition Peptide Fractions Enzymatic Hydrolysis

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Synergistic effect of mucuna pruriens and Withania somnifera in a paraquat induced Parkinsonian mouse model

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Advances in Bioscience and Biotechnology 2013年第11期4卷 1-9页

作者： Jay Prakash Satyndra Kumar Yadav Shikha Chouhan Satya Prakash Surya Pratap Singh 1Department of Biochemistry Faculty of Science Banaras Hindu University Varanasi India Biomedical Technology and Cell Therapy Research Laboratory Department of Biomedical Engineering Faculty of Medicine McGill University Montreal Canada Department of Biochemistry Faculty of Science Banaras Hindu University Varanasi India

Parkinson’s disease (PD) is a neurodegenerative disorder characterized by the development of rigidity, resting tremors and postural instability. Recently, the focus of PD’s treatment has shifted towards herbal medicines. mucuna pruriens (Mp) and Withania somnifera (Ws) are traditional herbal medicines known to have neuro-protective effects due to the L-DOPA present in Mp seed powder and withanoloides present in Ws root extract. Here, the synergistic effect of Mp and Ws in Parkinsonian mice induced by chronic exposure to paraquat was evaluated. Co-treatment with Mp and Ws for 9 weeks, significantly decreased the elevated nitrite levels and lipid peroxidation found in Parkinsonian mice. In behavioural tests, Mp and Ws treated mice showed a significant decrease in the time taken to cross a narrow beam, an increase in the time of stay on drum in rotarod test and an improvement in the hanging time. Furthermore, it was found that the use of Mp and Ws considerably improved the tyrosine hydroxylase expression in the substantianigra region of the brain. The results suggest that Mp and Ws may provide a platform for future drug discoveries and novel treatment strategies for PD.

关键词： Withinia somnifera mucuna pruriens Oxidative Stress Tyrosine Hydroxylase Parkinson’s Disease Substantia nigra Motor Dysfunctions

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Coag-Flocculation Kinetics of mucuna sloaneiSeed for Phosphorus Removal from Waste Water

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American Journal of Analytical Chemistry 2013年第12期4卷 732-738页

作者： Kamoru Akinpelu Babayemi Okechukwu Dominic Onukwuli Matthew Chukwudi Menkiti Akindele Oyetunde Okewale Department of Chemical Engineering Anambra State University Uli Nigeria Department of Chemical Engineering Landmark University Omuaran Nigeria Department of Chemical Engineering Nnamdi Azikiwe University Awka Nigeria

mucuna sloanei (MS) an environmentally friendly biomass was used as a coagulant for treatment of phosphorus containing waste water. The study evaluates the coag-flocculation efficiency of MS and its functional kinetic parameter response to varying pH and dosage of the waste water effluent. Coag-flocculation reaction order α, coag-flocculation rate constant K, and coagulation period τ1/2 were determined. The maximum coag-flocculation performance (97.4%) is recorded at rate constant, K of 1.24 × 10-4 l/mg·min, dosage of 400 mg/l, pH of 8 and coagulation period τ1/2 of 0.100 min while the minimum (61%) is recorded at K of 3 × 10-5 l/gm·min, dosage 100 mg/l, pH of 2 and τ1/2 of 8.900 mins. The results confirm that MS coagulant is an effective coagulant obeying the theory of fast coagulation in the conditions of the experiments.

关键词： Coag-Flocculation mucuna slonaei Phosphorus

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