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The construction of in vitro nasal cavity-mimic M-cell model,design of M cell-targeting nanoparticles and evaluation of mucosal vaccination by nasal administration

引用

Acta Pharmaceutica Sinica B 2020年第6期10卷 1094-1105页

作者： Xiaotong Yang Xianchun Chen Ting Lei Lin Qin Yang Zhou Chuan Hu Qingfeng Liu Huile Gao Key Laboratory of Drug Targeting and Drug Delivery System of the Education Ministry Sichuan Engineering Laboratory for Plant-Sourced Drug and Sichuan Research Center for Drug Precision Industrial TechnologyWest China School of Pharmacy Sichuan UniversityChengdu 610041China College of Materials Science and Engineering Sichuan UniversityChengdu 610064China Shanghai University of Medicine&Health Sciences Affiliated Sixth People's Hospital South Campus Shanghai 201499China

In order to better evaluate the transport effect of nanoparticles through the nasal mucosa,an in vitro nasal cavity-mimic model was designed based on M *** differentiation of M cells was induced by co-culture of Calu-3 and Raji cells in invert *** ZO-1 protein staining and the transport of fluorescein sodium and dexamethasone showed that the inverted co-culture model formed a dense monolayer and possessed the transport *** differentiation of M cells was observed by upregulated expression of Sialyl Lewis A antigen(SLAA)and integrinβ1,and down-regulated activity of alkaline *** targeting M cells with iRGD peptide(cRGDKGPDC),the transport of nanoparticles *** vivo,the co-administration of iRGD could result in the increase of nanoparticles transported to the brain through the nasal cavity after intranasal *** the evaluation of immune effect in vivo,the nasal administration of OVA-PLGA/iRGD led to more release of IgG,IFN-γ,IL-2 and secretory IgA(sIgA)compared with OVA@PLGA ***,the study constructed in vitro M cell model,and proved the enhanced effect of targeting towards M cell with iRGD on improving nasal immunity.

关键词： M cells In vitro Cell model iRGD peptide Nasal administration mucosal vaccination

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