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The inactivation of bacteriophages ms2 and PhiX174 by nanoscale zero-valent iron:Resistance difference and mechanisms

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Frontiers of Environmental Science & Engineering 2022年第8期16卷 247-256页

作者： Rong Cheng Yingying Zhang Tao Zhang Feng Hou Xiaoxin Cao Lei Shi Peiwen Jiang Xiang Zheng Jianlong Wang School of Environment and Natural Resources Renmin University of ChinaBeijing 100872China China Water Environment Group Co.Ltd. Beijing 101101China Institute of Nuclear and New Energy Technology Tsinghua UniversityBeijing 100084China

Pathogenic enteric viruses pose a significant risk to human health.Nanoscale zero-valent iron(nZVI),a novel material for environmental remediation,has been shown to be a promising tool for disinfection.However,the existing research has typically utilized ms2 or f2 bacteriophages to investigate the antimicrobial properties of nZVI,and the resistance difference between bacteriophages,which is important for the application of disinfection technologies,is not yet understood.Here,ms2 and PhiX174 containing RNA and DNA,respectively,were used as model viruses to investigate the resistances to nZVI.The bacteriophage inactivation mechanisms were also discussed using TEM images,protein,and nucleic acid analysis.The results showed that an initial concentration of 10^(6)PFU/mL of ms2 could be completely inactivated within 240 min by 40 mg/L nZVI at pH 7,whereas the complete inactivation of PhiX174 could not be achieved by extending the reaction time,increasing the nZVI dosage,or changing the dosing means.This indicates that the resistance of phage PhiX174 to nZVI was much stronger than that of ms2.TEM images indicated that the viral particle shape was distorted,and the capsid shell was ruptured by nZVI.The damage to viral surface proteins in both phages was examined by three-dimensional fluorescence spectrum and sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE).However,the nucleic acid analysis demonstrated that the nucleic acid of ms2,but not PhiX174,was destroyed.It indicated that bacteriophage inactivation was mainly attributed to the damage of nucleic acids.

关键词： Nanoscale zero-valent iron(nZVI) ms2 PhiΧ174 Resistance Inactivation Pathogenic microorganisms

Microwave-Assisted Inactivation of Fomite-Microorganism Systems: Energy Phase-Space Projection

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American Journal of Analytical Chemistry 2022年第7期13卷 255-276页

作者： Victor J. Law Denis P. Dowling School of Mechanical and Materials Engineering University College Dublin Dublin Ireland

This paper describes the use of log-linear energy phase-space projections to analyze microwave-assisted inactivation of bacteria and viruses under different fomite conditions within multimode microwave ovens. The ovens are operated at a cavity-magnetron frequency of 2.45 ± 01 GHz. Porous fomites (moist face towels, cotton swabs, kitchen sponges, and scrubbing pads, cigarette filters and N95-like respirators);along with non-porous hard surface syringe fomites are studied. The fomites are classed as dielectric;and absorb microwave energy to varying degrees depending on their complex dielectric permittivity. Microorganism resilience to microwave stress (defined as ≥4 log10 reduction in inactivation) when mapped using iso-volume trend-lines in energy phase-space reveals the persistence imparted by the fomite, and can be mapped between different microwave ovens. Microorganism resilience to thermal microwave-assisted treatment increases from vegetative Gram-negative to vegetative Gram-positive and on to Gram-positive spores. Bacteriophage ms2 and influenza viruses have an intermediate resilience dependency. It is shown that linear-scaled fomite temperature against process time graphs can differentiate between non-thermal and thermal micro-wave-assisted treatment of microorganisms.

关键词： Bacteria ms2 Hepatitis C Virus Human Immunodeficiency Virus Influenza Virus Disruption Inactivation Microwave Oven Data Analysis

气态臭氧对不同食品和包装材料上3种模式噬菌体的消毒效果评价

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食品安全质量检测学报 2023年第20期14卷 236-244页

作者：陈君然彭艳艳李海川张静敏胡开蕾胡云峰天津科技大学食品科学与工程学院天津300457

目的采用φ6、φX174和ms23种被广泛接受的模式噬菌体评价气态臭氧对食品和包装材料的消毒效果。方法以食品、包装材料及其不同模式噬菌体为研究对象,从臭氧通入量、处理时间、模式噬菌体对数减少及其消杀效果等方面评价其对食品及包装... 详细信息

目的采用φ6、φX174和ms23种被广泛接受的模式噬菌体评价气态臭氧对食品和包装材料的消毒效果。方法以食品、包装材料及其不同模式噬菌体为研究对象,从臭氧通入量、处理时间、模式噬菌体对数减少及其消杀效果等方面评价其对食品及包装材料的影响。结果气态臭氧对除花生仁外的各种食品中的φ6有很好的去除效果[降低大于1.6个对数级(log),灭活大于97.00%],对花生仁上的φX174和ms2的影响很小[降低小于0.19个对数级(log),灭活50.00%],吸附在大豆上的病毒替代品被有效消毒(降低大于1.16个对数级);气态臭氧能有效灭活包装材料上的φ6、φX174和ms2,明显降低牛皮纸、塑料和玻璃板上φ6和ms2的含量[降低大于0.83个对数级(log),灭活大于84.44%],适度降低φX174的含量。结论气态臭氧可以有效地去除φ6、φX174和ms2,但对不同材料上不同模式噬菌体的灭活效果存在一定的差异。

关键词：臭氧替代病毒 φ6 φX174 ms2 病毒灭活

BREEDING OF DWARFING-STERILE WHEAT AND ITS POTENTIAL VALUES IN WHEAT BREEDING

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Chinese Science Bulletin 1991年第18期36卷 1562-1564页

作者：刘秉华杨丽 Institute of Crop Breeding and Cultivation Chinese Academy of Agricultural Sciences Beijing 100081 PRC

A male sterile form of common wheat(Triticum aestivum) was found in China and it has been shown that the sterility is controlled by a single, dominant gene. The dominant ms2 gene for male sterility (originally Tal assigned by Deng and Gao, 1982)is located on the short ann of chromosome 4D. This ms2 gene has been extensively used as a

关键词： ms2 gene for male sterility and Rht10 gene for dwarfness close linkage dwarfing-sterile wheat.

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气-水界面对病毒静态吸附实验结果的影响

环境科学 2007年第12期28卷 2800-2805页

作者：张辉赵炳梓张佳宝张丛志王秋英陈吉中国科学院南京土壤研究所土壤与农业可持续发展国家重点实验室封丘农田生态系统国家试验站

气-水界面存在可能导致不能正确评价土壤对病毒的静态吸附能力.本研究通过室内一次平衡法实验,比较分析气-水界面对噬菌体ms2在不同处理(非灭菌/灭菌)的不同土壤中的吸附实验结果影响,同时评价不同程度气-水界面对没有土壤存在时的病毒... 详细信息

气-水界面存在可能导致不能正确评价土壤对病毒的静态吸附能力.本研究通过室内一次平衡法实验,比较分析气-水界面对噬菌体ms2在不同处理(非灭菌/灭菌)的不同土壤中的吸附实验结果影响,同时评价不同程度气-水界面对没有土壤存在时的病毒回收率影响.结果表明,气-水界面存在对吸附实验结果的影响在砂质潮土上最为明显,其次为红壤土,而在红粘土上的影响几乎可忽略,这可能与土壤本身吸附/致死病毒的能力不同有关;土壤灭菌后进一步加剧气-水界面对砂质潮土吸附实验结果的偏差,而在红壤土上则有减弱趋势.气-水界面存在显著降低空白实验病毒回收率,其降低趋势随气-水界面的增加而加剧,当加入土壤后,土壤颗粒有可能阻止病毒接近气-水界面或改变病毒吸附在固-液-气界面的力量,因而气-水界面存在时的空白实验结果并非为真正的空白实验结果,这可能是导致气-水界面引起静态吸附实验结果偏差的主要原因.

关键词： ms2 土壤气-水界面静态吸附

易组“太谷核不育基因”(ms2 )基因定位的研究

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Acta Genetica Sinica 2002年第12期29卷 1085-1094页

作者：傅大雄阮仁武温海霞陈云平宗学凤殷家明戴秀梅张建奎西南农业大学农学及生命科学学院重庆400716

将在远缘杂交中由普通小麦 (AABBDD) 4D染色体易组导入六倍体小黑麦 (AABBRR)以及硬粒小麦 (AABB)的太谷核不育基因ms2 (原位于普通小麦 4D染色体短臂距着丝点 31.2cM的显性雄性不育核基因 )重新导回普通小麦染色体组中。所获携带易组ms... 详细信息

将在远缘杂交中由普通小麦 (AABBDD) 4D染色体易组导入六倍体小黑麦 (AABBRR)以及硬粒小麦 (AABB)的太谷核不育基因ms2 (原位于普通小麦 4D染色体短臂距着丝点 31.2cM的显性雄性不育核基因 )重新导回普通小麦染色体组中。所获携带易组ms2基因的新型太谷核不育小麦其显性雄性不育特性表达正常 ,且雄性不育株的雌性可育机制正常 ,对不育株幼穗花粉母细胞减数分裂期染色体构型的观察可见其为整倍体 (2n =4 2 ) ,尚未发现回归普通小麦的易组太谷核不育基因与原位点的太谷核不育基因有不同的表型。采用系统的标志基因测交法对回归普通小麦的易组太谷核不育基因进行了测交定位 ,发现易组ms2基因与普通小麦显性矮秆标志基因Rht3连锁 ,从而将其定位于普通小麦 4B染色体短臂距Rht3基因 9.7cM处 ,新位点被命名为ms2 (4BS)。对ms2基因在六倍体小黑麦与原太谷核不育小麦远缘杂交中易位时的走向 ,普通小麦 4A与 4B染色体的互换更名以及ms2 (4BS)新位点的开发利用进行了讨论 :认为异源多倍体生物核基因的组间易位倾向于从供体染色体向进化亲缘关系较密切 ,且染色体序数与染色体臂相同的部分同源染色体易位 ;1988年第 7届国际小麦遗传学会对普通小麦 4A与 4B染色体的互换更名是正确的 ;ms2 (4BS)

关键词：太谷核不育基因 ms2 基因定位小麦 Rht3 组间易位

Mathematical Approach to the Platonic Solid Structure of ms2 Particles

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Applied Mathematics 2015年第4期6卷 655-662页

作者： Nai-Chia Shi Chun-Chun Wu Jui-Sen Yang Department of Applied Mathematics Feng-Chia University Taichung Taiwan Institute of Marine Biology National Taiwan Ocean University Keelung Taiwan

Bacteriophage ms2 is a viral particle whose symmetrical capsid consists of 180 copies of asymmetrical coat proteins with triangulation number T = 3. The mathematical theorems in this study show that the phage particles in three-dimension (3D) might be an icosahedron, a dodecahedron, or a pentakis dodecahedron. A particle with 180 coat protein subunits and T = 3 requires some geometrical adaptations to form a stable regular polyhedron, such as an icosahedron or a dodecahedron. However, with mathematical reasons electron micrographs of the phage ms2 show that 180 coat proteins are packed in an icosahedron. The mathematical analysis of electron micrographs in this study may be a useful tool for surveying the platonic solid structure of a phage or virus particle before performing 3D reconstruction.

关键词： ms2 Icosahedron Mathematical Approach

Effect of Post-Treatment Conditions on the Inactivation of ms2 Bacteriophage as Indicator for Pathogenic Viruses after the Composting Process

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Journal of Agricultural Chemistry and Environment 2018年第2期7卷 73-80页

作者： Hamidatu S. Darimani Ryusei Ito Naoyuki Funamizu Amadou H. Maiga School of Engineering Wa Polytechnic Upper West Region Ghana Faculty of Engineering Hokkaido University Sapporo Japan Water and Climate Centre International Institute for water and Environmental Engineering (2iE) Ouagadougou Burkina Faso

A rural model composting toilet system still had some pathogens in the compost after months of operation and hence requires a post-treatment. The aim of the study was to sanitize compost withdrawn from the composting toilet by setting post-treatment conditions. The kinetics inactivation of ms2 bacteriophage, selected as indicator for pathogenic viruses were determined during post-treatment at different temperatures (30°C, 40°C and 50°C) with varying moisture contents (50%, 60% and 70%). As a result, the inactivation rates during the post-treatment were 0.093 - 0.020 h-1, 0.025 - 0.088 h1, 0.447 - 0.100 h-1 at 30°C, 40°C and 50°C respectively. The inactivation rate coefficient (k) values of ms2 bacteriophage depended on higher temperature but not on moisture content.

关键词： Composting Toilet ms2 Bacteriophage Temperature Moisture Content Post-Treatment

构建ms2-RIP方法用于鉴定lncRNA DANCR在肿瘤细胞中的相互作用分子

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中山大学学报（医学科学版） 2021年第1期42卷 24-32页

作者：巫孟师熊敏敏彭丹韩雪钟小敏中山大学中山医学院干细胞与组织工程研究中心广东广州510080

【目的】为了探讨lncRNA DANCR在肿瘤细胞中的作用机制,本研究拟构建一种新的ms2-RIP方法用于鉴定DANCR的相互作用分子。【方法】利用ms2噬菌体衣壳蛋白可与噬菌体复制酶编码基因5’端一段由19个碱基组成的RNA茎环结构序列,即ms2结合位... 详细信息

【目的】为了探讨lncRNA DANCR在肿瘤细胞中的作用机制,本研究拟构建一种新的ms2-RIP方法用于鉴定DANCR的相互作用分子。【方法】利用ms2噬菌体衣壳蛋白可与噬菌体复制酶编码基因5’端一段由19个碱基组成的RNA茎环结构序列,即ms2结合位点(ms2 Binding Site,ms2 BS)高效结合的特点,构建串联表达ms2 BS和DANCR的过量表达质粒,通过ms2蛋白与ms2 BS的亲和作用,使DANCR得到富集,并且同步捕获与DANCR相互作用的分子,用于进一步分析鉴定。【结果】成功构建串联表达ms2 BS和DANCR的过量表达质粒用于ms2-RIP实验,该实验可显著富集DANCR以及作为阳性对照的DANCR结合蛋白EZH2。【结论】基于ms2蛋白与ms2结合位点的高效结合特性,我们针对lncRNA DANCR建立了ms2-RIP方法。该方法可有效富集DANCR以及与DANCR结合的生物活性分子,为研究DANCR的作用机制提供一种新的技术手段。

关键词： ms2 RNA结合蛋白免疫沉淀长链非编码RNA DANCR