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Effects of electro-acupuncture on brain tissue norepinephrine contents in a morphine withdrawal anxiety mouse model

Neural Regeneration Research 2008年第4期3卷 402-405页

作者： Qizhi Zhou Yuxing Liu Xuguang Liu Jiaolu Wei Yong Tang Junmei Wu Yi Pu Acupuncture ＆ Tuina College Chengdu University of Traditional Chinese Medicine Chengdu 610075 Sichuan Province China

BACKGROUND： Following morphine withdrawal, anxiety is associated with abnormal norepinephrine content change. However, increasing blood lactic acid content can induce anxiety or panic in patients with anxiety disorder or endogenous opioid peptide functional disorder. OBJECTIVE： This study was designed to observe the effects of electro-acupuncture, at the ＂Sanyinjiao＂ point （SP 6）, on brain tissue norepinephrine and blood lactic acid content in anxiety-model mice after morphine withdrawal. DESIGN： A randomized controlled animal experiment. SETTING： This study was performed in the Laboratory of Acupuncture, Electro-acupuncture ＆ Tuina College, Chengdu University of Traditional Chinese Medicine, from June to September 2001. MATERIALS： A total of 50 healthy Kunming male mice were provided by the Laboratory Animal Center of Chengdu University of Traditional Chinese Medicine. The protocol was performed in accordance with ethical guidelines stated in the Guide for the use and care of laboratory animals, approved by the Committee on the Care and Use of Laboratory Animals of the Institute of Laboratory Animal Resources Commission on Life Sciences, National Research Council, China （1985）. Experimental reagents and equipment used were as follows： morphine hydrochloride （Lot No. 930503, Shenyang No.1 Pharmaceutical Factory, China）, norepinephrine （Sigma Chemical Company, USA）, fluorospectrophotometer （RF-510, Shimadzu Corporation, Japan）, Han electro-acupuncture apparatus （WQ 1002, No. zun （91）-227270-588, Beijing Anlong Photoelectricity-Technique Company, China）, and T-maze （self-made）. METHODS： A total of 50 mice were randomly divided into 5 groups, with 10 mice in each group： blank control, T-maze, model, model ＋ electro-acupuncture, and electro-acupuncture groups. Establishment of anxiety model after morphine withdrawal： the mouse hot plate assay was used to detect the activity of morphine. The median effective dose of morphine, 2.95 mg/kg, was defined as the base. Mice wer

关键词： morphine withdrawal anxiety norepinephrine lactic acid acupuncture

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Parvalbumin Interneurons of Central Amygdala Regulate the Negative Affective States and the Expression of Corticotrophin-Releasing Hormone during morphine withdrawal

Parvalbumin Interneurons of Central Amygdala Regulate the Ne...

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中国神经科学学会第十二届全国学术会议

作者： Xue-Ying WANG Li WANG Min-Jie SHEN Chang-You JANG Fei-Fei WANG Lan MA State Key Laboratory of Medical Neurobiology School of Basic Medical Sciences and Institutes of Brain Science and the Collaborative Innovation Center for Brain ScienceFudan University Department of Assisted Reproduction Shanghai Ninth People's Hospital Affiliated Shanghai Jiao Tong University School of Medicine

Objective The central nucleus of the amygdala（CeA） is a crucial component of the neuronal circuitry mediating aversive emotion. Its role in the negative affective states during drug withdrawal includes changes in opioidergic, GABAergic, and corticotropin-releasing factor neurotransmission. However, the modulation of the neurobiological interconnectivity in the CeA and its effects in the negative reinforcement of drug dependents are poorly understood. The study is to investigate the specific interaction mechanism of PV+ interneurons and corticotropin-releasing hormone（CRH） in the CeA during and their impact on morphine withdrawal. Methods We performed electrophysiological recordings to assess the membrane excitability of parvalbumin（PV）+ interneurons in the CeA during chronic morphine withdrawal. We tested the morphine withdrawal–induced negative affective states, such as the aversive（assessed by conditioned place aversion）, anxiety（assessed by elevated plus maze）, and anhedoniclike（assessed by saccharin preference test） behaviors, as well as the mRNA level of CRH via optogenetic inhibition or activation of PV+ interneurons in the CeA. Results Chronic morphine withdrawal increased the firing rate of CeA PV+ interneurons. Optogenetic inhibition of the activity of CeA PV+ interneurons attenuated the morphine withdrawal–induced negative affective states, such as the aversive, anxiety, and anhedonic-like behaviors, while direct activation of CeA PV+ interneurons could trigger those negative affective-like behaviors. Optogenetic inhibition of the CeA PV+ interneurons during the morphine withdrawal significantly attenuated the elevated CRH mRNA level in the CeA. Conclusion The activity of PV+ interneurons in the CeA was up-regulated during chronic morphine withdrawal. The activation of PV+ interneurons during morphine withdrawal was crucial for the induction of the negative emotion and the up-regulation of CRH mRNA levels in the CeA.

关键词： central amygdala CRH morphine withdrawal negative affective states PV

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Effects of morphine dependence and withdrawal on sensory information processing in cerebellar granule cell layer in vivo in mice

Effects of morphine dependence and withdrawal on sensory inf...

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中国神经科学学会第十二届全国学术会议

作者： Li-jie Zhan Bing-xue Li Dan Wang De-lai Qiu Yan Lan Cellular Function Research Center Yanbian University Department of Physiology and Pathophysiology College of Medicine Yanbian University Department of pharmacology College of Medicine Yanbian University Key Laboratory of Natural Resource of the Changbai Mountain and Functional Molecular of the Ministry of Education Yanbian University

Background: It has been reported that the mechanisms of morphine addiction is the homeostasis dysregulation of brain. The cerebellum is involved in control of motor coordination and receives sensory inputs to execute complex motor tasks. Increasing evidence has involved that addiction is related to a disruption in cerebellar processing and connectivity with other brain regions. However, the changes of information processing in cerebellum in morphine addiction is unknown. In present research, our aim is to observe the effects of morphine dependence and withdrawal on sensory information processing in cerebellar granule cell layer in vivo in mice. Methods and Main Results: Forty male KM mice were randomly divided into four groups（n=10）: the morphine dependence group, morphine withdraw 1 day group, morphine withdrawal 7 days group, and the saline control group. The mice in dependence group and withdrawal group were administered with morphine by subcutaneous injection through the method of "increasing addiction for 7 days", and the saline group were injected saline. Mice administered morphine exhibited opioid-like responses including Straub tail and hyperlocomotion in behavior. Electrophysiological methods were used in our research to record the field potential of granule cell layer（GCL）. Air-puff stimulation of the ipsilateral whisker-pad evoked stimulus-on（N1） and stimulus-off responses（N2） in GCL of cerebellar Crus II. The amplitude of N1 and N2 in dependence group, withdrawal 1 day group and withdrawal 7 day group are obviously decreased compared with control group（p＜0.05）, and withdrawal 1 day group shows a marked drop compared with dependence group and withdrawal 7 day group（p＜0.05）. The onset latency of the sensory stimulationevoked responses has no change. Conclusion: These results indicate that morphine dependence and withdrawal affect the homeostatic regulation of sensory information processing in cerebellar granule cell layer.

关键词： morphine dependence morphine withdrawal Cerebellar granule cell Mice Sensory stimulation

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