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中国神经科学学会第十二届全国学术会议

作者： Li-jie Zhan Bing-xue Li Dan Wang De-lai Qiu Yan Lan Cellular Function Research Center Yanbian University Department of Physiology and Pathophysiology College of Medicine Yanbian University Department of pharmacology College of Medicine Yanbian University Key Laboratory of Natural Resource of the Changbai Mountain and Functional Molecular of the Ministry of Education Yanbian University

Background: It has been reported that the mechanisms of morphine addiction is the homeostasis dysregulation of brain. The cerebellum is involved in control of motor coordination and receives sensory inputs to execute complex motor tasks. Increasing evidence has involved that addiction is related to a disruption in cerebellar processing and connectivity with other brain regions. However, the changes of information processing in cerebellum in morphine addiction is unknown. In present research, our aim is to observe the effects of morphine dependence and withdrawal on sensory information processing in cerebellar granule cell layer in vivo in mice. Methods and Main Results: Forty male KM mice were randomly divided into four groups（n=10）: the morphine dependence group, morphine withdraw 1 day group, morphine withdrawal 7 days group, and the saline control group. The mice in dependence group and withdrawal group were administered with morphine by subcutaneous injection through the method of "increasing addiction for 7 days", and the saline group were injected saline. Mice administered morphine exhibited opioid-like responses including Straub tail and hyperlocomotion in behavior. Electrophysiological methods were used in our research to record the field potential of granule cell layer（GCL）. Air-puff stimulation of the ipsilateral whisker-pad evoked stimulus-on（N1） and stimulus-off responses（N2） in GCL of cerebellar Crus II. The amplitude of N1 and N2 in dependence group, withdrawal 1 day group and withdrawal 7 day group are obviously decreased compared with control group（p＜0.05）, and withdrawal 1 day group shows a marked drop compared with dependence group and withdrawal 7 day group（p＜0.05）. The onset latency of the sensory stimulationevoked responses has no change. Conclusion: These results indicate that morphine dependence and withdrawal affect the homeostatic regulation of sensory information processing in cerebellar granule cell layer.

关键词： morphine dependence morphine withdrawal Cerebellar granule cell Mice Sensory stimulation

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Brain-derived neurotrophic factor and Bcl-2 expression in rat brain areas following chronic morphine treatment

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Neural Regeneration Research 2011年第7期6卷 528-533页

作者： Huiping Yu Hua HU Huaqing Meng Wei Deng Yixiao Fu Qinghua Luo Mental Health Center First Affiliated Hospital Chongqing Medical University Chongqing 400016 China Department of Neurology Leshan People's Hospital Leshan 614000 Sichuan Province China

The ventral tegmental area and the locus coeruleus are associated with psychological and physical dependence of opioid addiction. To date, very little is known about brain-derived neurotrophic factor （BDNF） and Bcl-2 gene and protein changes following morphine addiction. The present study utilized immunohistochemistry and in situ hybridization techniques, which revealed that there were increased BDNF levels, but decreased Bcl-2 levels in the prefrontal cortex, locus coeruleus, hippocampus, and the ventral tegmental area during morphine-dependence formation and abstinence. However, the levels of BDNF remained unchanged, and Bcl-2 expression was increased in the nucleus accumbens. These results showed that BDNF and Bcl-2 are involved in the development of morphine dependence, and precipitation of abstinence syndrome.

关键词： morphine dependence substance withdrawal syndrome brain-derived neurotrophic factor Bcl-2 brain injury neural regeneration

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维普期刊数据库

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Sidiming attenuates morphine withdrawal syndrome and nitric oxide (synthase) levels in morphine-dependent rats and rhesus monkeys

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Neural Regeneration Research 2011年第33期6卷 2589-2593页

作者： Zheng Yang Renbin Huang Jianchun Huang Shijun Zhang Xing Lin Yang Jiao Weizhe Jiang Department of Pharmacology Guangxi Medical University Nanning 530021 Guangxi Zhuang Autonomous Region China

The present study analyzed the effects of Sidiming, a Chinese herbal compound, on withdrawal syndrome, body weight loss, and serum levels of nitric oxide and its synthase in morphine- dependent rats and rhesus monkeys. These effects were compared with clonidine, an active control drug used for clinical treatment. Results showed that 4 and 8 g/kg Sidiming, respectively, significantly suppressed morphine withdrawal syndrome and reduced body mass loss in morphine-dependent rats. In addition, 2.4 and 4.8 g/kg Sidiming, respectively, significantly attenuated withdrawal syndrome in rhesus monkeys. High-dose Sidiming （8 g/kg in rats and 4.8 g/kg in rhesus monkeys） led to significantly inhibited serum levels of nitric oxide and its synthase in morphine-dependent rats and rhesus monkeys, which were greater than clonidine. These findings suggested that Sidiming treatment attenuated withdrawal syndrome in morphine-dependent rats and rhesus monkeys by inhibiting serum nitric oxide and its synthase.

关键词： morphine dependence nitric oxide nitric oxide synthase opioid rhesus monkey Sidiming withdrawal syndrome

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Changes of Adenylate Cyclase and Guanylate Cyclase in the Frontal Cortex,Lenticula,Corpus Amygdaloideum,and Hippocampus in morphine‑dependent Rats

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Journal of Forensic Science and Medicine 2016年第1期2卷 1-7页

作者： Shijun Hong Dongxian Zhang Lihua Li School of Forensic Medicine Kunming Medical UniversityKunmingYunnanPeople’s Republic of China

To detect the changes of adenylate cyclase(AC)and guanylate cyclase(GC)in the four cerebral regions that are concerned with psychogenic dependence of morphine in rats,including the frontal cortex,lenticula,corpus amygdaloideum,and *** discuss the relation between the expressions of AC and GC with the psychogenic dependence on *** periods of morphine‑dependent rat models were established,and enzyme histochemistry was used to detect the variations of AC and GC in four cerebral *** with the control group,AC and GC in all the morphine‑dependent groups *** data indicated that the amounts of AC and GC were significantly different between the morphine‑dependent groups and the control group when tested at periods of 1 week,2 weeks,4 weeks,and 8 weeks(Pmorphine‑dependent *** psychogenic dependence on morphine appears to be closely linked to the upgrade of AC and GC.

关键词： Adenylate cyclase cerebral regions guanylate cyclase morphine dependence

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Dopamine enhances morphine-induced increase of pCREB in caudate nucleus of rats

Dopamine enhances morphine-induced increase of pCREB in caud...

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中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Ying ZHANG Man-Ying XU~* Guang-Wen ZHANG He-Ren GAO Duo ZHANG Department of Physiology Harbin Medical University Harbin 150081 China

＜正＞Objective morphine dependence leads to adaptive reactions in the central nervous system that occur at the cellular and molecular *** of the molecular mechanisms of addiction is the upregulation of the cAMP second messenger pathway,which occurs in many neuronal cell types in response to chronic administration of opiates or other drugs of *** CREB phosphorylation by morphine has been described in many cell types and in many brain regions,including striatal *** mesolimbic dopaminergic system is the primary substrate of opioid *** present study is to explore whether dopamine affects the pCREB level in morphine-dependence *** pCREB level was examined by immunohistochemistry after injection into cerebral ventricle with saline or *** Our data demonstrated that pCREB level increased during morphine ***,Dopamine enhanced morphineinduced argument of CREB phosphorylation in caudate *** This study suggested that Dopamine might regulate signal transduction enhanced by morphine dependence.

关键词： dopamine morphine dependence cAMP response element binding protein

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Effect of different durations of morphine exposure on mesencephalic dopaminergic neurons in morphine dependent rats

Effect of different durations of morphine exposure on mesenc...

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中国神经科学学会第十届全国学术会议

作者： Wei-Bo SHI Jing-Na JIA Qian QI Shu-Jin DU Chun-Ling MA Ying-Min LI Bin CONG Institute of Basic Medicine Hebei Medical University

Objective Mesencephahc dopaminergic neurons are heavily involved in the development and maintenance of drug ***,the morphological changes in dopaminergic neurons of the substantia nigra（SN） and ventral tegmental area（VTA） in morphine dependent rats were *** Models of morphine dependence were estabhshed in rats,and paraffin-embedded sections,immunohistochemistry and immunofluorescence were used to observe the changes in expression of tyrosine hydroxylase（TH） protein,a specific marker of dopaminergic nerve ***-Jade B staining was used to detect degeneration and necrosis,and terminal deoxynucleotidyl transferase-mediated dUTP nick-end-labeling（TUNEL） detected apoptosis of mesencephahc dopaminergic nerve *** The number of TH positive cells significantly decreased in rats that were morphine dependent for a longer period of *** prolonged morphine exposure,SN and VTA dopaminergic nerve cells showed degeneration and necrosis,while apoptotic cells were not *** The number of SN and VTA dopaminergic nerve cells decreased with increasing periods of morphine dependence,which was most likely due to degeneration and necrosis of nerve cells induced by morphine toxicity.

关键词： morphine dependence substantia nigra ventral tegmental area TH-positive cell Fluoro-Jade B

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吗啡依赖大鼠海马、纹状体及皮层组织的蛋白质组分析

吗啡依赖大鼠海马、纹状体及皮层组织的蛋白质组分析

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2005年全国有机质谱学术交流会

作者：何家田王翔赵兴红吴宁刘炳玉苏瑞斌魏晓丽魏开华李锦王红霞军事医学科学院国家生物医学分析中心兰州军区联勤部药品仪器检验所军事医学科学院毒物药物研究所

越来越多的观点认为阿片依赖的神经生物学基础是神经元的代偿性适应,这种适应性改变涉及很多脑区及许多分子。目前为止,与阿片依赖相关的差异蛋白质表达谱的研究尚未见报道。本研究的目的是用蛋白质组学技术在海马、纹状体及皮层脑区寻... 详细信息

越来越多的观点认为阿片依赖的神经生物学基础是神经元的代偿性适应,这种适应性改变涉及很多脑区及许多分子。目前为止,与阿片依赖相关的差异蛋白质表达谱的研究尚未见报道。本研究的目的是用蛋白质组学技术在海马、纹状体及皮层脑区寻找与阿片依赖相关的差异表达的蛋白质。通过凝胶图像分析和统计学分析,确定与吗啡依赖相关的差异蛋白质斑点;用基质辅助激光解吸电离飞行时间质谱技术和电喷雾电离串联质谱技术,鉴定差异蛋白质。希望能为深入研究阿片依赖的神经生物学机制提供全新的线索。

关键词： Proteomics morphine dependence Plasticity Acetylation

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