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Establishment of Human Neuroblastoma Cell-Line (SK-N-SH) as an In Vitro Model of morphine addiction

Journal of Pharmacy and Pharmacology 2021年第3期9卷 115-121页

作者： Noor Azuin Suliman Noor Azlina Abu Bakar Mohamad Aris Mohd Moklas Che Norma Mat Taib Faculty of Medicine and Health Science Universiti Sultan Zainal Abidin(UniSZA)Kampus KotaJalan Sultan MahmudKuala Terengganu20400Malaysia Department of Biomedical Sciences Faculty of Medicine and Health SciencesUniversiti Putra Malaysia43400 UPM SerdangSelangorMalaysia

morphine is a schedule II-controlled substance that used to allow the diminution of intra-operative, post-operative orchronic pain. However, its usage is limited due to addiction and overdose liabilities. morphine was observed to cause tolerance,dependence and withdrawal in human. Justification: to date lack of scientific evidence of morphine addiction was carried out byusing specific single human neuroblastoma cell-line (SK-N-SH). Therefore, this study was performed to establish the morphineaddiction model in this cell line. The cells were exposed to morphine for 24 hrs before treatment with methadone, as ananti-withdrawal drug for subsequence 24 hours. The cytosolic fraction of the cell was used in different objectives including receptoraffinity, withdrawal properties, endocytic machinery, desensitisation or internalisation and cellular adaptation. The result shows thatmorphine and methadone bind to the μ-opioid receptor. The morphine-treated cells were observed to increase the expression ofaddiction markers, have a low rate of the endocytic machinery, cause desensitisation of receptor and reduce cellular *** changes by morphine were normalised by the treatment of methadone. As a whole, it is postulated that neuroblastoma cell line,SK-N-SH, can be used as an in-vitro model to demonstrate morphine addiction before animal and human testing.

关键词： morphine addiction receptor affinity withdrawal properties endocytic machinery desensitisation internalisation cellular adaptation methadone.

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PGE2 as a morphine-Seeking Behavior Modulator in the Place Preference Model

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Journal of Behavioral and Brain Science 2022年第12期12卷 627-639页

作者： Ariadna Jiménez-González Abraham Ochoa-Aguilar Katia Mendoza-Negrete Leticia Parra-Gamez Claudia Gómez-Acevedo Department of Pharmacology Behavioral Pharmacology Laboratory Facultad de Medicina Universidad Nacional Autónoma de México (UNAM) México City México Hospital Loma Linda Translational Research Laboratory Naucalpan México Department of Anatomy Facultad de Medicina Universidad Nacional Autónoma de México (UNAM) México City México

The opioid epidemic has become one of the most concerning public health issues in the world, and currently does not have an adequate treatment available. It has been observed that, despite opioids being highly addictive, patients with chronic inflammation are less likely to develop an opioid dependence. This protective effect may be caused by Prostaglandin E2 (PGE2) as it has been found that non-painful carrageenan inflammation reduces morphine induced reward. Taking this into account, the aim of this study was to determine if the direct administration of PGE2 into the central nervous system could modulate the morphine-induced reward. We used the morphine-conditioned place preference (CPP) model with and without PGE2 or PGE2R antagonist in order to test the reward response. We found a significant reduction of morphine-induced reward after administering PGE2. Moreover, we found that this effect could be reversed by PGE2 receptor antagonism. Our data suggest that PGE2 may reduce morphine-induced reward making it an important drug-target research alternative to explore the possibility of modifying or even preventing opioid addiction.

关键词： morphine addiction Conditioned Place Preference Rats Prostaglandins Immune System Reward

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Effect of acetylcholine and locus ceruleus on the algaesthesis modulation of morphinistic rats

Effect of acetylcholine and locus ceruleus on the algaesthes...

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中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Yu LIANG~1,Guang-Wen ZHANG~2,Bo WU~3,Yi-Fan DENG~3,Duo ZHANG~3,He-Ren GAO~3,Run-Sheng JIAO~3,Man-Ying XU~(3,*) 1 Mudanjiang Medical College,Mudanjiang 157000,China 2 Third Affiliated Hospital 3 Laboratory of Neural Electrophysiology,Department of Physiology,Harbin Medical University,Harbin 150081, China

＜正＞Objective At the level of the central pain-related neurons and receptors,the experiment observed the the role and the interaction of acetylcholine（ACh） and locus ceruleus（LC） in the modulation of the algaesthesis of morphinistic *** The electrophysiological method and intracerebroventricular（icv） injection of drugs was used in the *** sciatic nerve was stimulated by a train of electrical impulses as noxious *** electric activities of pain-related neurons in LC were led out by the *** experiment observed the effects of ACh,atropine or pilocarpine on the electric activities of the pain-excitation neurons（PEN） and pain-inhibition neurons（PIN） in LC of morphinistic ***（1） The icv injection of ACh（20μg/10μl） increased the pain-evoked discharged frequency and shortened the latency of PEN,and decreased the pain-evoked discharged frequency and prolongated of the inhibitory duration of PIN in LC of morphinistic rats.（2） The M-receptor antagonist atropine（5μg/10μl） which injected into icv could block the effects of ACh.（3） The effects of M-receptor agonist pilocarpine（5μg/10μl） on PEN and PIN were similar to the effects of *** The exogenous ACh or pilocarpine could strengthen the response of pain-related neurons in LC of morphinistic rats to the noxious *** the effects of facilitated *** atropine could weaken the effects of ACh on PEN and PIN,expressing as analgesic *** is exposed that the facilitated painful effects of ACh achieved by the M-receptor,and the ACh and LC play a very important roles in the modulation of algaesthesis.

关键词： acetylcholine morphine addiction locus ceruleus pain-related neurons electric activities

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