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Surrogate Role of CD85k on monocytic Lineage Involved Leukemogenesis Biology and Clinical Aspect

Open Journal of Blood Diseases 2018年第4期8卷 61-73页

作者： Hasnaa A. Abo-Elwafa Shereen P. Aziz Heba A. Ahmed Elsayed Mostafa Ali Doaa S. Elsaied Clinical Pathology Department Sohag Faculty of Medicine Sohag University Sohag Egypt Clinical Oncology Department Sohag Faculty of Medicine Sohag University Sohag Egypt

Background: Unique receptor involved in leukemogenesis is CD85k;an immuneglobulin receptor for immune tolerance, CD36 is glycoprotein mediates cellular adhesion and metastatic spread, CD14, CD15 considered common monocytic markers. Aims: to investigate CD85k with monocytic lineage involved leukemia (MLIL) markers in leukemia pathogenesis and clinical presentation. Patients and Methods: 47 patients (32 diagnosed acute myeloid leukemia (AML);15 non-malignant hematological disease as a control), were included, aged from 2 to 80 years, all subjected to peripheral blood (***) and bone marrow (B.M) examination, immunophenotyping (IPT) using FASC Canto four color flow cytometer (FCM) Becton Dickenson (BD) USA, for CD13, CD33, MPO, HLA-DR, CD34, CD38, CD117, CD14, CD15 and CD36 the Mo Abs supplied by B.D Bioscience, and anti CD85k Mo Abs by Aveda de Coimbra Flamenco, reference No. 1399990130. Results: Frequency of CD85k is 19/32 (59.37%) of AML;14/14 (M4/M5) 100% positive CD85k, insignificant correlations of CD85k to sex, lymphadenopathy or organomegaly, platelets count and *** blast (P > 0.05), significant to age 50,000 × 109/l, Hb 0.05). Conclusion: Although CD85k is MLIL associated marker, it is not correlated with other MLIL markers with frequency 100% in MLIL and 59.37% in AML, age predisposition is <35 years with no sex variation, significant correlation to progenitor and myeloid markers, it’s a crucial role in leukemogenesis biology, not in clinical presentations, considered good follow up predictor MLIL marker.

关键词： CD85k monocytic Lineage Leukemia

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Bioinformatic prediction and functional characterization of human KIAA0100 gene

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Journal of Pharmaceutical Analysis 2017年第1期7卷 10-18页

作者： He Cui Xi Lan Shemin Lu Fujun Zhang Wanggang Zhang Department of Clinical Hematology Affiliated No.2 Hospital Xi'an Jiaotong University Department of Biochemistry and Molecular Biology School of Basic Medical Sciences Xi'an Jiaotong University Health Science Center

Our previous study demonstrated that human KIAA0100 gene is a novel acute monocytic leukemia-associated antigen(MLAA) gene. But the functional characterization of human KIAA0100 gene has remained unknown to date. Here, firstly, bioinformatic prediction of human KIAA0100 gene was carried out using online software;Secondly, human KIAA0100 gene expression was downregulated by the clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated(Cas) 9 system in U937 cells. Cell proliferation and apoptosis were next evaluated in KIAA0100-knockdown U937 cells. The bioinformatic prediction showed that human KIAA0100 gene was located on 17q11.2, and human KIAA0100 protein was located in the secretory pathway. Besides, human KIAA0100 protein contained a signal peptide, a transmembrane region, three types of secondary structures(alpha helix, extended strand, and random coil), and four domains from mitochondrial protein 27(FMP27). The observation on functional characterization of human KIAA0100 gene revealed that its downregulation inhibited cell proliferation, and promoted cell apoptosis in U937 cells. To summarize, these results suggest human KIAA0100 gene possibly comes within mitochondrial genome; moreover, it is a novel anti-apoptotic factor related to carcinogenesis or progression in acute monocytic leukemia, and may be a potential target for immunotherapy against acute monocytic leukemia.

关键词： Human KIAA0100 gene Bioinformatic prediction Acute monocytic leukemia associated antigen CRISPR/Cas9 system Cell proliferation Cell apoptosis

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