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Advanced oxidation protein products induce monocyte chemoattractant protein-1 expression via p38 mitogen-activated protein kinase activation in rat vascular smooth muscle cells

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Chinese Medical Journal 2006年第13期119卷 1088-1093页

作者： PENG Kan-fu WU Xiong-fei ZHAO Hong-wen SUN Yan Department of Nephrology Southwest Hospital Third Military Medical University Chongqing 400038 China

Background Advanced oxidation protein products （AOPPs） are new uremic toxins reported by Witko-Sarsat in 1996, which are associated with the pathogenesis of atherosclerosis. However, the mechanisms by which AOPPs enhance atherosclerosis have not been fully understood. monocyte chemoattractant protein-1 （MCP-1） is a chemokine which stimulates migration of monocytes and plays a critical role in the development of atherosclerosis. In this study, we investigated the effect of AOPPs on MCP-1 expression in cultured vascular smooth muscle cells （VSMCs）.

关键词： atherosclerosis advanced oxidation protein products monocyte chemoattractant protein-1 mitogen-activated protein kinase myocytes, smooth muscle

Urotensin Ⅱ promotes monocyte chemoattractant protein-1 expression in aortic adventitial fibroblasts of rat

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Chinese Medical Journal 2014年第10期127卷 1907-1912页

作者： Zhang Yonggang Bao Shilin Kuang Zejian Ma Yanjun Hu Yanchao Mao Yanyan Department of Cardiology Second Affiliated Hospital ShantouUniversity Medical College Shantou Guangdong 515041 China Cardiovascular Laboratory Centre for Translational MedicineShantou University Medical College Shantou Guangdong 515041China Department of Cardiovascular Diseases First Affiliated Hospital Shantou University Medical College Shantou Guangdong 515041 China

Background Urotensin Ⅱ （Ull）,a potent vasoconstrictive peptide,is able to stimulate phenotypic differentiation of adventitial *** study aimed to determine the effect of UII on monocyte chemoattractant protein-1 （MCP1） expression in rat aortic adventitial fibroblasts,so as to explore possible mechanisms in the development of vascular *** Growth-arrested adventitial fibroblasts were incubated in serum-free medium with UII （1010-10-7 mol/L） and inhibitors of signal transduction pathways for 1 to 24 ***-1 mRNA and protein expression and secretion were determined by RT-PCR,Western blotting analysis and enzyme-linked immunosorbent assay （ELISA）,*** UII dose-and time-dependently promoted MCP-1 mRNA and protein expression and secretion in cells,with maximal effect at 10-8 mol/L at 3 hours for mRNA expression,24 hours for protein expression in the cells,and 12 hours for protein secretion from the ***,the UII effects were significantly inhibited by treatment with its receptor antagonist SB710411,Rho kinase inhibitor Y27632,protein kinase C （PKC） inhibitor H7,mitogen-activated protein kinase inhibitor PD98059,calcineurin inhibitor cyclosporine A,and the Ca2＋channel blocker *** UII may stimulate MCP-1 expression in rat aortic adventitial fibroblasts through its receptor and Rho kinase,PKC,mitogen-activated protein kinase,calcineurin and Ca2＋ channel signal transduction,thus contributing to adventitial inflammation.

关键词： urotensin Ⅱ monocyte chemoattractant protein-1 adventitial fibroblasts signal transduction

Variation of Serum monocyte chemoattractant protein-1 in Patients with Diabetes and Metabolic Syndrome

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2011年第3期31卷 312-316页

作者：黎慧清邓秀玲李贞琼罗长青刘建社王玉梅 Department of Endocrinology Union HospitalTongji Medical CollegeHuazhong University of Sci-ence and Technology Department of Nephrology Union HospitalTongji Medical CollegeHuazhong University of Sci-ence and Technology

This study investigated the variation of serum monocyte chemoattractant protein-1（MCP-1） in patients with both diabetes mellitus（DM） and metabolic syndrome（MS）.Based on the International Diabetes Federation（IDF） diagnostic criteria,93 patients enrolled in this study were divided into four groups：normal control（NC）,simple DM,simple MS,and DM plus MS（DM-MS） *** main measures included height,weight,waist circumference（WC）,hip circumference,blood pressure,fasting blood glucose,insulin resistance index（HOMA-IR）,serum triglyceride（TG）,HDL-ch,LDL-ch,and *** results showed that the serum levels of MCP-1 in the DM-MS group were significantly increased as compared with those in the DM and MS groups（P0.05）,and the increase in the MCP-1 level in the DM group was much higher than in the MS group（P0.05）.The DM-MS group had the highest HOMA-IR levels,followed by MS,DM and NC groups（P0.05）.Correlation tests showed that the association of MCP-1 with age,HDL-ch,or LDL-ch was insignificant,whereas that of MCP-1 with body mass index（BMI）,waist hip rate（WHR）,WC,systolic blood pressure（SBP）,diastolic blood pressure（DBP）,TG,and HOMA-IR was significantly *** was concluded that circulating MCP-1 was substantially increased in patients with both DM and MS as compared with that in the patients with DM or MS alone,and the central obese state may contribute to a more vicious proinflammatory condition and insulin resistance in patients with diabetes.

关键词： monocyte chemoattractant protein-1 diabetes metabolic syndrome

monocyte chemoattractant protein-1 plays a key role in type 1 diabetes

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Journal of Nanjing Medical University 2005年第2期19卷 60-62页

作者： DongLi GuoliangLiu DepartmentofInfectiousDisease theFirstAffifiatedHospitalofChinaMedicalUniversityShenyang110001China 2DepartmentofEndocrinology theFirstAffifiatedHospitalofChinaMedicalUniversityShenyang110001China

Type 1 diabetes is an autoimmune dise as e resulting from the selective destruction of β cells in the pa ncreatic islets. In both human and rodent models of type 1 diabetes, the clinica l disease is preceded by a progressive mononuclear cell invasion of the pancreat ic islets (insulitis). In the early stage of insulitis,the major components are monocyte/macrophages, and the recruitment of mononuclear cells is a critical st ep in the pathogenesis of the type 1 diabetes. Studies have revealed that Monocy te chemoattractant protein-1(MCP-1) specifically recruits monocytes/ macrophag es into pancreas and plays an important role in the development of insulitis and diabetes.

关键词： monocyte chemoattractant protein-1 insulits type 1 diabetes

Homocysteine induces production of monocyte chemoattractant protein-1 and interleukin-8 in cultured human whole blood

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Acta Pharmacologica Sinica 2004年第11期 35-41页

作者： Daniel G REMICK Ann Arbor Department of Pathology University of Michigan Medical School MI 48109 USA

AIM: To investigate whether increased plasma L-homocysteine (Hcy) level could promote monocyte chemoattractant protein-1 (MCP-1) and interleukin-8 (IL-8) in cultured whole blood. METHODS: Human whole blood or different type of peripheral blood cells from health volunteers were incubated with Hcy and/or the inhibitors. MCP-1 and IL-8 level were measured by ELISA assay. RESULTS: Hcy 10-1000μmol/L induced production of MCP-1 and IL-8 in cultured human whole blood (Pmonocytes. Meanwhile,Hcy also promoted the upregulation of MPO level even at the 10 μmol/L in the cultured whole blood. The intracellular ROS, particular the OH’ radicals, play extremely important role in the Hcy-induced MCP-1 and IL-8 production. CONCLUSION: Increased Hcy level in plasma (hyperhomocysteinemia) induced MCP-1 and IL-8 secretion in cultured human whole blood, especially in monocytes via oxidative stress mechanism.

关键词： atherosclerosis monocyte chemoattractant protein-1 interleukin-8 homocysteine monocytes

Effects of Simvastatin on NF-κB-DNA Binding Activity and monocyte chemoattractant protein-1 Expression in a Rabbit Model of Atherosclerosis

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2006年第2期26卷 194-198页

作者：杨晓云王琳曾和松 DUBEY Laxman 周宁卜军 Department of Cardiology Tongji Medical College Huazhong University of Science and Technology Wuhan 430030China

To observe the effects of simvastatin on nuclear factor kappaB （NF-kB）-DNA binding activity and on the expression of monocyte chemoattractant protein-1 （MCP-1） in atherosclerotic plaque in rabbits and to explore the anti-atherosclerotic properties beyond its lipid-lowering effects. Thirty-six New Zealand male rabbits were randomly divided into low-cholesterol group （LC）, high- cholesterol group （HC）, high-cholesterol＋ simvastatin group （HC＋S） and then were fed for 12 weeks. At the end of the experiment, standard enzymatic assays, electrophoretic mobility shift as- say （EMSA）, immunohistochemical staining, and morphometry were performed to observe serum lipids, NF-kB-DNA binding activity, MCP-1 protein expression, intirna thickness and plaque area of aorta respectively in all three groups. Our results showed that the serum lipids, NF-kB-DNA binding activity, expression of MCP-1 protein, intima thickness, and plaque area of aorta in the LC and HC＋S groups were significantly lower than those in the HC group （P〈0.05）. There was no significant difference in the serum lipids between the LC and HC＋S groups （P〉0.05）, but the NF-kB-DNA binding activity, the expression of MCP-1 protein and the intirna thickness and plaque area of aorta in the HC＋S group were significantly decreased as compared to the LC group （P〈0. 05）. This study demonstrated that simvastatin could decrease atherosclerosis by inhibiting the NF-kB-DNA binding activity and by reducing the expression of MCP-1 protein.

关键词： simvastatin nuclear factor kappaB monocyte chemoattractant protein-1 atherosclerosis

Expression of monocyte chemoattractant protein-1 in monocytes and Effects of Native and Oxidized Very Low Density Lipoproteins

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Journal of Huazhong University of Science and Technology(Medical Sciences) 1997年第4期17卷 203-205页

作者：王国平邓仲端倪娟 Department of Pathology Tongji Medical University Wuhan

monocyte chemoattractant protein-1(MCP-1), a potent chemoattractant, is thought to play an important role in migration of monocytes into atherosclerotic lesions. The present study was designed to investigate the capacity of human peripheral blood monocytes to express MCP-1 and effects of native very low density lipoprotein (VLDL) and oxidized VLDL(OX-VLDL) on the expression. The total RNA was extracted from cultured monocytes, which were exposed to VLDL and OX-VLDL, and the media conditioned by monocytes were collected. MCP-1 mRNA expression was examined by Northern blot analysis. MCP-1 protein in conditioned media was determined by using sandwich ELISA. The results showed that monocytes can express MCP-1 after a 24 h incubation at 37℃，and the expression was markedly increased by a exposure to OX-VLDL, whereas the expression was slightly increased when exposed to VLDL. It suggests that the capacity of monocytes to produce MCP-1 that recruits and activates circulating monocytes may be of considerable importance in atherogenesis, and oxidation of VLDL enhances its potential to promote atherogenesis.

关键词： monocyte chemoattractant protein-1 very low density lipoprotein oxidization monocytes atherosclerosis

The enhancement of astrocytic-derived monocyte chemoattractant protein-1 induced by the interaction of opiate and HIV tat in HIV-associated dementia

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Journal of Pharmaceutical Analysis 2009年第4期21卷 277-281页

作者： Xiao Han Biomedical Experimentation,School of Basic Medical Sciences,Peking University Health Science Center,Beijing 100191,China Biomedical Experimentation School of Basic Medical Sciences Peking University Health Science Center Beijing 100191 China

HIV-associated dementia(HAD)is a public health problem and is particularly prevalent in drug *** neuropathogenesis of human immunodeficiency virus(HIV)infection involves a complex cascade of inflammatory events,including monocyte/macrophage infiltration in the brain,glial immune activation and release of neurotoxic *** these events,astrocytic-derived monocyte chemoattractant protein-1(MCP-1)plays an important role,whose release is elevated by HIV transactivator of transcription(HIV tat)and could be further elevated by *** review will also consider some critical factors and events in MCP-1 enhancement induced by the interactions of opiate and HIV tat,including the mediating role of mu opioid receptor(MOR)and CCR2 as well as the possible signal transduction pathways within the ***,it will make some future perspectives on the exact pathways,new receptors and target cells,and the vulnerability to neurodegeneration with HIV and opiates.

关键词： dementia HIV transactivator of transcription astrocyte morphine monocyte chemoattractant protein-1

Effects of Wenxiao Decoction(稳消方) on the Expression of Interleukin-6,Intercellular Adhesion Molecular-1and monocyte chemoattractant protein-1 in Experimental Atherosclerotic Rabbits

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Chinese Journal of Integrative Medicine 2014年第6期20卷 445-449页

作者：霍清萍梁芳李金菩王宇新刘含嫣 Department of Traditional Chinese Medicine Shanghai No.6 People's HospitalAffiliated to Shanghai Jiaotong University

Objective： To observe the effects of different doses of Wenxiao Decoction （稳消方） on the expression of interleukin-6 （IL-6）, intercellular adhesion molecule-1 （ICAM-1）, and monocyte chemoattractant protein-1 （MCP-1） in experimental atherosclerotic rabbits and to explore the mechanism by which it alleviates atherosclerosis. Methods： Sixty New Zealand rabbits were randomly divided into six groups： a blank group, a model group, a Simvastatin group, and high-, medium-, and low-dosage Wenxiao Decoction groups. Except for those in the blank group, all rabbits were fed with a high-cholesterol diet. Carotid atherosclerosis was established by balloon-induced carotid artery endothelium injury in conjunction with the high-cholesterol diet. After 8 weeks, all animals were euthanized to evaluate levels of IL-6 and ICAM-1 expressions （by enzyme linked immunosorbent assay） and of MCP-1 （by immunohistochemistry staining）. Results： The expressions of IL-6, ICAM-1, and MCP-1 were significantly increased in all groups except the blank group （P〈0.05）. However, the rabbits in the Wenxiao Decoction groups and the Simvastatin group showed significantly lower levels of IL-6, ICAM-1, and MCP-1 expression than those in the model group （P〈0.05）. The expressions of IL-6, ICAM-1, and MCP-1 in the high- dosage Wenxiao Decoction group and the Simvastatin group were lower than those in the low-dosage Wenxiao Decoction group （P〈0.05）. The expression of MCP-1 in medium-dosage Wenxiao Decoction group was lower than that in the low-dosage group （P〈0.05）. Conclusions： High, medium, and low doses of Wenxiao Decoction can inhibit the expressions of IL-6, ICAM-1, and MCP-1, which may prevent and stabilize atherosclerotic plaques. There may be a direct relationship between dosage and therapeutic efficacy of Wenxiao Decoction.

关键词： Wenxiao Decoction atherosclerosis interleukin-6 intercellular adhesion molecular-1 monocyte chemoattractant protein-1 Chinese medicine