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Morphological, Cultural, molecular and Pathogenic variation of Bipolaris sorokiniana in Wheat

American Journal of molecular Biology 2022年第4期12卷 190-202页

作者： A. Sharmin F. M. Aminuzzaman T. H. Ansari M. Ahmed Department of Plant Pathology Faculty of Agriculture Sher–e–Bangla Agricultural University Dhaka Bangladesh Plant Pathology Division Bangladesh Rice Research Institute (BRRI) Gazipur Bangladesh

One hundred and twenty isolates of Bipolaris sorokiniana of wheat were isolated, identified and characterized based on morphological, cultural, pathogenic and ITS (Internal Transcribed Spacer) based molecular parameters. Collected isolates differed in respect of radial mycelial growth rate, color of the colony, surface texture of the colony, shape of the colony, conidia production ability and shape and color of conidia. ITS1 and ITS4 based PCR products yielded a species-specific band of 600 bp (base pairs) on agarose gel for the confirmation of B. sorokiniana. The mycelial growth rate of the isolates ranged from 1.39 to 4.46 mm/day. The septation of conidia ranged from 1.4 to 8.6. On the basis of conidia morphology, the isolates were grouped into five different groups, whereas the isolates were grouped into 12 cultural groups based on cultural characteristics. Cultural group 8 [CG (Cultural Group)-8] was the most virulent [38.5% LAD (Leaf area diseased)] and [CG (cultural Group)-4] was the less virulent [2% LAD (Leaf area diseased)] group among 12 groups when inoculated at tillering stage of wheat cv. Kachan.

关键词： B. sorokiniana Wheat Morphological variation Cultural variation Molecular variation Virulence

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Incidence,genomic diversity,and evolution of strawberry mottle virus in China

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BIOCELL 2021年第4期45卷 1137-1151页

作者： LINGJIAO FAN CHENGYONG HE MENGMENG WU DEHANG GAO ZHENFEI DONG SHENGFAN HOU ZEKUN FENG HONGQING WANG College of Horticulture China Agricultural UniversityBeijing100193China

Strawberry mottle virus(SMoV)is one of the most common viruses infecting strawberries,causing losses to fruit yield and quality.In this study,165 strawberry leaf samples were collected from six provinces of China,46 of which tested positive for SMoV.The complete genome sequences of 11 SMoV isolates were obtained from Liaoning(DGHY3,DGHY16-2,DGHY17,DGHY20-2,DGHY21,DGHY26-2),Shandong(SDHY1,SDHY5,SDHY31-2,SDHY33-2),and Beijing(BJMX7).The RNA1 and RNA2 nucleotide identities between the 11 Chinese isolates were 95.4-99.3%and 96.3-99.6%,respectively,and they shared 78.4-96.6%and 84.8-93.5%identities with the available SMoV isolates in GenBank.Recombination analysis revealed that Chinese isolate SDHY33-2 and Canadian isolates Ontario and Simcoe were recombinants,and recombination events frequently occurred in the 3’UTR of SMoV.Phylogenetic analysis showed that in an RNA1 tree,most Chinese isolates clustered into the same group while isolate DGHY17 clustered into another group together with Czech isolate C and three Canadian isolates.In an RNA2 tree,all Chinese isolates clustered into a single group.The phylogenetic analysis based on nucleotide sequences was consistent with the results based on coat protein(CP)and RNA-dependent RNA polymerase(RdRp).Further evolutionary analysis indicated that negative selection drives SMoV evolution,and gene flow plays a major role in genetic differentiation.Additionally,reassortment and recombination also influence the evolution of SMoV.To our knowledge,this is the first report of the complete genome of SMoV isolates from China and a detailed analysis of the SMoV population structure.

关键词： Viral occurrence Complete genome Molecular variation Genetic structure

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Genetic variation analysis of Apple stem grooving virus coat protein in Beijing

Genetic variation analysis of Apple stem grooving virus coat...

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中国植物病理学会2015年学术年会

作者： CHEN Hui FAN Zai-feng GUO Li-yun ZHOU Tao Department of Plant Pathology China Agricultural University

Apple stem grooving virus(ASGV) is one of the most important viral pathogens infecting various pome(apple,quince and pear) and stone(peach,plum,apricot) fruit trees worldwide.In this study,the complete sequences of coat protein(CP) gene of 22 isolates of ASGV from Beijing were determined.Molecular variation and phylogenetic analysis in ASGV CP sequences worldwide were analyzed.Beijing ASGV isolates among themselves and with isolates from elsewhere in the world shared88.9%100%and 87.1%99.7%sequence identities at the amino acid and nucleotide levels,respectively.Multiple sequence alignment of 22 CP amino acid sequences showed that the codons at positions 48,104,108 are the mainvariationsites,which is consistent with other isolates of worldwide.Selectionpressure results suggest that most of the codons are under neutral evolution.Phylogenetic analysis revealed that these CP sequences separated into two clusters regardless of the geographic origin or host plants.

关键词： Apple stem grooving virus Molecular variation phylogenetic analysis

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