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molecular methods for detection of pathogenic viruses of respiratory tract——A review

Asian Pacific Journal of Tropical Biomedicine 2018年第5期8卷 237-244页

作者： Md.Shahidul Kabir Department of Microbiology Institutional Quality Assurance Cell Stamford University

Human respiratory system is harbored by a vast array of transient and normal microbial flora.A number of pathogenic viruses were diagnosed from samples in different occasions from mild to severe infections of respiratory tract.molecular methods were developed for detection of these viruses during last two decades.Nucleic acid amplification methods were introduced for rapid and accurate diagnosis of pathogenic viruses.Multiplex detection systems were employed to identify a panel of pathogenic viruses,which requires specialized kits and instruments in some cases.This review summarizes different types of molecular approaches which were developed with time and applied for the detection of pathogenic viruses associated with infections of the respiratory system.

关键词： Respiratory system Virus molecular method Detection

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Isolation and Identification of Bacteria Found in the Milt of Cultured Clarias gariepinus

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Journal of Fisheries Science 2022年第2期4卷 9-14页

作者： Folalu Adekunle Awe Ayofe Mutalib Hammed Olamiwaye Akintade Adeboyejo Olusegun Olufemi Whenu Oluwatosin Modupe Olarinmoye Gabriel Olarinde Mekuleyi Jesuyon Joseph Hungbo Akanji Adeolu Akinyemi Olufunmilayo Adebimpe Olanloye Department of Fisheries Faculty of ScienceLagos State UniversityOjoLagos StateNigeria Department of Aquaculture and Fisheries Management College of Environmental Resources ManagementFederal University of AgricultureAbeokutaNigeria Department of Pure and Applied Botany College of BiosciencesFederal University of AgricultureAbeokutaNigeria

This study investigated bacteria that are associated with milt in cultured samples of African catfish-Clarias gariepinus.Male broodstocks of Clarias gariepinus were collected from private Fish Farm in Ogun State,Nigeria,while the fish’s milt was collected and analyzed for microbial load at laboratory of the Department of Microbiology,Federal University of Agriculture,Abeokuta,Ogun State.The molecular characterization of the amplicon was done at International Institute of Tropical Agriculture,Ibadan.Bio-edit was used for importing and mining nucleotide sequences into gene bank.As revealed by the results,bacterial organisms that were present in the milt of Clarias gariepinus are Aeromonas caviae,Proteus mirabilis,Serratia rubidaea,Pseudomonas mosselii,Acinetobacter soli and Klebsiella variicola.The Basic Local Alignment Search Tools revealed the percentage similarity ranging from 86%-97.04%and their accession numbers.These bacteria indicated high levels of faecal contamination in the environment.In conclusion,bacteria were found in the milt of cultured catfish and are capable of being pathogenic to humans and may increase the vertical transfer to fry during breeding and rearing stage.Therefore,fish farmers should maintain a hygiene and serene environment during breeding and culturing of catfish.

关键词： Milt Clarias gariepinus Bacteria molecular method

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