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A new species in the genus Malcus Stål(Hemiptera:Heteroptera:Malcidae)from China based on morphological and molecular data

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Entomotaxonomia 2020年第4期42卷 252-257页

作者： Shujing WANG Wenjun BU College of Life Sciences Nankai UniversityTianjin 300071China

Morphological and molecular data confirm a new species in genus Malcus from Xizang,*** zhengi *** described and *** and illustrations of the adult,critical morphological characteristics and pygophore are provided.

关键词： Lygaeoidea morphology molecular data taxonomy

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molecular Clump Extraction Algorithm Based on Local Density Clustering

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Research in Astronomy and Astrophysics 2022年第1期22卷 19-29页

作者： Xiaoyu Luo Sheng Zheng Yao Huang Shuguang Zeng Xiangyun Zeng Zhibo Jiang Zhiwei Chen Center for Astronomy and Space Sciences China Three Gorges UniversityYichang 443000China Purple Mountain Observatory Chinese Academy of SciencesNanjing 210023China Xinjiang Astronomical Observatory Chinese Academy of SciencesUrumqiXinjiang 830011China College of Electrical Engineering and New Energy China Three Gorges University8 University RoadYichang443000China

The detection and parameterization of molecular clumps are the first step in studying *** propose a method based on the Local Density Clustering algorithm while physical parameters of those clumps are measured using the Multiple Gaussian Model *** advantage of applying the Local Density Clustering to the clump detection and segmentation,is the high accuracy under different signal-to-noise *** Multiple Gaussian Model is able to deal with overlapping clumps whose parameters can reliably be *** simulation and synthetic data,we have verified that the proposed algorithm could accurately characterize the morphology and flux of molecular *** total flux recovery rate in 13CO(J=1-0)line of M16 is measured as 90.2%.The detection rate and the completeness limit are 81.7%and 20 K km s-1 in 13CO(J=1-0)line of M16,respectively.

关键词： molecular data molecular processes methods:laboratory:molecular

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Direct comparison of culture-dependent and culture-independent molecular approaches reveal the diversity of fungal endophytic communities in stems of grapevine(Vitis vinifera)

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Fungal Diversity 2018年第3期90卷 85-107页

作者： Asha J.Dissanayake Witoon Purahong Tesfaye Wubet Kevin D.Hyde Wei Zhang Haiying Xu Guojun Zhang Chunyuan Fu Mei Liu Qikai Xing Xinghong Li Jiye Yan Institute of Plant and Environment Protection Beijing Academy of Agriculture and Forestry SciencesNo.9 of Shuguanghuayuan ZhongluHaidian DistrictBeijing 100097China Beijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China Beijing Academy of Agriculture and Forestry SciencesNo.9 of Shuguanghuayuan ZhongluHaidian DistrictBeijing 100097China Center of Excellence in Fungal Research Mae Fah Luang University333 MuangChiang Rai 57100Thailand Department of Soil Ecology UFZ-Helmholtz Centre for Environmental ResearchHalle(Saale)Germany Beijing Academy of Forestry and Pomology Sciences No.12 of XiangshanruiwangfenBeijing 100093China German Centre for Integrative Biodiversity Research(iDiv)Halle-Jena-Leipzig LeipzigGermany

Grapevines(Vitis vinifera)are colonized by ubiquitous microorganisms known as endophytes,which may have advantageous or neutral effects without causing disease *** endophytes are uncultivable,so culture-independent approaches such as next generation sequencing(NGS)can help for a better understanding of their ecology and *** date,there are no studies which directly link NGS results with taxa derived from a culturing approach,integrating morphological and multi-gene phylogenetic analysis of *** this study,a culture-dependent and high-resolution culture-independent approach(next generation sequencing)were used to identify endophytes in grapevine *** the culture-dependent approach,a total of 94 isolates were recovered from 84 of 144 healthy grapevine stem fragments(colonization rate=58.3%).The study is unique as we used subsets of combined multi-gene regions to identify the endophytes to species *** on each multi-gene phylogenetic analysis,28 species belong to 19 genera(Acremonium,Alternaria,Arthrinium,Ascorhizoctonia,Aspergillus,Aureobasidium,Bipolaris,Botryosphaeria,Botrytis,Chaetomium,Cladosporium,Curvularia,Hypoxylon,Lasiodiplodia,Mycosphaerella,Nigrospora,Penicillium,Phoma,Scopulariopsis)were identified.A higher number of culturable fungi were obtained from 13 year-old vines,followed by eight and three yearold *** the culture-independent approach,a fungal richness of 59 operational taxonomic units(OTU)was detected,being highest in 13 year-old grapevines,followed by eight and three *** though the cultivation approach detected lower fungal richness,the results related to stem are consistent for fungal community composition and *** of the fungal taxa identified by the two approaches resulted in an overlap of 53%of the fungal *** to interspecific variability of the sequences from NGS,in many cases the OTUs(even with the highly abundant ones)were only assignable to order,family or genus

关键词： molecular data Morphological characteristics Multi-gene phylogeny Mycobiome Next generation sequencing

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Epitypification and neotypification:guidelines with appropriate and inappropriate examples

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Fungal Diversity 2014年第6期69卷 57-91页

作者： Hiran A.Ariyawansa David L.Hawksworth Kevin D.Hyde E.B.Gareth Jones Sajeewa S.N.Maharachchikumbura Dimuthu S.Manamgoda Kasun M.Thambugala Dhanushka Udayanga Erio Camporesi Anupama Daranagama Ruvishika Jayawardena Jian-Kui Liu Eric H.C.McKenzie Rungtiwa Phookamsak Indunil C.Senanayake Roger G.Shivas Qing Tian Jian-Chu Xu Key Laboratory for Plant Diversity and Biogeography of East Asia Kunming Institute of BotanyChinese Academy of ScienceKunming 650201YunnanChina World Agroforestry Centre East and Central AsiaKunming 650201YunnanChina Institute of Excellence in Fungal Research School of ScienceMae Fah Luang UniversityChiang Rai 57100Thailand Departamento de Biología Vegetal II Facultad de FarmaciaUniversidad Complutense de MadridPlaza Ramón y CajalMadrid 28040Spain Department of Life Sciences The Natural History MuseumCromwell RoadLondon SW75BDUK Mycology Section Royal Botanic GardensKewRichmond Surrey TW93DSUK Department of Botany and Microbiology King Saudi UniversityRiyadhSaudi Arabia A.M.B.Gruppo Micologico Forlivese“Antonio Cicognani” Via Roma18ForlìItaly Manaaki Whenua Landcare Research Private Bag 92170 AucklandNew Zealand Plant Pathology Herbarium Agri-Science Queensland40 Boggo RoadDutton ParkQld 4102Australia

review of phylogenetic studies carried out together with morphological ones shows that a major problem with most early studies is that they concentrated on techniques and used material or strains of fungi that in most cases were not carefully reference,and in a worrying number of cases wrongly *** classical species,particularly of microfungi,are not represented by adequate type material,or other authoritatively identified cultures or specimens,that can serve as DNA sources for phylogenetic study,or for developing robust identification *** classifications of fungi therefore suffer fromthe lack of reference strains in resultant phylogenetic *** some cases,epitypification and neotypification can solve this problem and these tools are increasingly used to resolve taxonomic confusion and stabilize the understanding of species,genera,families,or orders of *** manuscript discusses epitypification and neotypification,describes how to epitypify or neotypify species and examines the importance of this process.A set of guidelines for epitypification is *** where taxa have been epitypified are presented and the benefits and problems of epitypification are *** examples of epitypification,or to provide reference specimens,a new epitype is designated for Paraphaeosphaeria michotii and reference specimens are provided for Astrosphaeriella stellata,***,Phaeosphaeria elongata,Ophiobolus cirsii,and *** this way we demonstrate how to epitypify taxa and its importance,and also illustrate the value of proposing reference specimens if epitypification is not *** we provided guidelines for epitypification,the decision to epitypify or not lies with the author,who should have experience of the fungus *** responsibility is to be taken seriously,as once a later typification is made,it may not be possible to undo that,particularly in the case of epitypes,without using the lengthy and tedious f

关键词： Epitype Generic types molecular data Nomenclature Systematics Taxonomy Typification

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A new species of the genus Coccophagus(Hymenoptera:Aphelinidae)associated with Sphaerolecanium prunastri(Hemiptera:Coccoidea)from the Tianshan Mountains,Xinjiang

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Entomotaxonomia 2022年第3期44卷 228-239页

作者： Zirao LI Lan HUANG Wenxia ZHAO Yanxia YAO Key Laboratory of Forest Protection of the National Forestry and Grassland Administration/Ecology and Nature Conservation Institute Chinese Academy of ForestryBeijing 100091China

To investigate the natural parasitoid enemies of Sphaerolecanium prunastri,a scale insect which harms wild fruit forests in western Tianshan,Xinjiang,branches seriously damaged by *** were collected and cultured in glass jars for obtaining natural *** tianshanensis Li&Yao ***.,a new species of Coccophagus(Hymenoptera:Aphelinidae)was gained and identified based on the morphological and molecular *** characteristics of *** are described in detail,and COI and 28S sequences are *** addition,a key to species of the genus Coccophagus in the world parasitizing *** was *** investigation has found a potential natural enemy of the *** in the wild fruit forests of the West Tianshan Mountains,Xinjiang,and establishes a new natural enemy for biocontrol of this pest.

关键词： parasitoid morphology molecular data taxonomy

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