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Graphene as adsorbent for highly efficient extraction of modified nucleosides in urine prior to liquid chromatography-tandem mass spectrometry analysis

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Chinese Chemical Letters 2024年第4期35卷 490-495页

作者： Cheng Guo Xiaoxiao Zhang Xiujuan Hong Yiqiu Hu Lingna Mao Kezhi Jiang Cancer Institute(Key Laboratory of Cancer Prevention and Intervention China National Ministry of Education)The Second Affiliated HospitalZhejiang University School of MedicineHangzhou 310009China Cancer Center Zhejiang UniversityHangzhou 310058China Key Laboratory of Organosilicon Chemistry and Material Technology College of Material Chemistry and Chemical EngineeringHangzhou Normal UniversityHangzhou 311121China Department of General Practice The Second Affiliated HospitalZhejiang University School of MedicineHangzhou 310009China

RNA modifications have been involved in numerous biological processes, and aberrations of these modifications are tightly associated with various diseases including cancer. Herein, we developed graphenebased solid-phase extraction and robust ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) combined with stable isotope-dilution for simultaneous enrichment and accurate determination of 17 modified nucleosides in human urine. We found graphene could effectively adsorb various modified nucleosides in human urine samples. With this method, we identified and quantified these modified nucleosides in urine samples collected from lung cancer patients and healthy *** revealed that the levels of 12 modified nucleosides were all diminished in urine from lung cancer patients, compared with healthy controls. It is worth noting that we demonstrated, for the first time, the presence of 5,2-O-dimethyluridine(m~5U_m) in human urine. Together, we established a robust analytical method for simultaneous determinations of 17 modified nucleosides in human urine, and our results revealed a close correlation between the concentrations of urinary modified nucleosides and the occurrence of lung cancer, implying the potential applications of these modified nucleosides as noninvasive biomarkers for the early detection of lung cancer. Moreover, this study will stimulate future investigations on the regulatory roles of RNA modifications in the initiation and progression of lung cancer.

关键词： Mass spectrometry RNA modification modified nucleosides Graphene Biomarker Urine

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A Noninvasive Cancer Detection Strategy Based on Gold Nanoparticle Surface-enhanced Raman Spectroscopy of Urinary modified nucleosides Isolated by Affinity Chromatography

A Noninvasive Cancer Detection Strategy Based on Gold Nanopa...

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第十九届全国光散射学术会议

作者： Shangyuan Feng Rong Chen Key Laboratory of Opto Electronic Science and Technology for Medicine Ministry of Education and Fujian Provincial Key Laboratory for Photonics Technology Fujian Normal University

＜正＞The search for tumor biomarkers in the urine f or cancer diagnosis is currently a hot topic in clinical oncology,with potential for cancer screening and *** nucleosides excreted through the urine are considered

关键词： Non-invasive cancer detection SERS spectroscopy affinity chromatography separation modified nucleosides biomarker

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Synthesis and cytotoxic evaluation of substituted 3-(3'-indolyl-/3'-pyridyl)-isoxazolidines and bis-indoles

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Acta Pharmaceutica Sinica B 2012年第1期2卷 32-41页

作者： Vishal Sharma Raman Kalia Tilak Raj Vivek K.Gupta Nitasha Suri Ajit K.Saxena Deepak Sharma Surinderjit S.Bhella Gurpinder Singh Mohan Paul S.Ishar Bio-Organic and Photochemistry Laboratory Department of Pharmaceutical SciencesGuru Nanak Dev UniversityAmritsar 143005India Pharmacology Division Indian Institute of Integrative MedicineJammu 180001India Post-Graduate Department of Physics University of JammuJammu Tawi 180006India

Regio-and stereoselective 1,3-dipolar cycloadditions of C-(3-indolyl)-N-phenylnitrone(10)were carried out with different mono-substituted,disubstituted and cyclic dipolarophiles under mono-mode microwave irradiation to obtain substituted 3-(indol-30-yl)-N-phenyl-isoxazolidines(16–22).Reactions of nitrone(10)with allenic esters under similar conditions afforded,via a domino process,bis-indole derivatives(23a–c)along with compounds 24 and ***,reactions of C-(3-pyridyl)-N-phenylnitrone(26)with mono-substituted,disubstituted and cyclic dipolarophiles were carried out in refluxing dry toluene to obtain substituted 3-(30-pyridyl)-Nphenylisoxazolidines(27–34).Some of the compounds(16f,18b,23a,23c,27c and 29f)display significant cytotoxicity against a number of human cancer cell lines.

关键词： 3-Indolylisoxazolidines 3-Pyridylisoxazolidines Bis-indoles 1,3-Dipolar cycloadditions modified nucleosides Cytotoxic activity

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Function of Transfer RNA Modifications in Plant Development

Function of Transfer RNA Modifications in Plant Development

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植物分子生物学与现代农业——2010全国植物生物学研讨会

作者： Peng Chen~*,Yong Gao,Ran Zuo,Gongke Zhou QingDao Institute of Bioenergy and Bioprocessing Technology,Chinese Academy of sciences, 266101,QingDao,ShanDong Province,P.R.China

Discovery of small RNA and fast progress in epigenetic study has proved the importance of modified nucleosides for the function of biological activity of small RNAs[1].Small RNA is important for plant growth and devel... 详细信息

关键词： Transfer RNA modified nucleosides plant development

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