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Immunophenotyping of hematopoietic progenitor cells: Comparison between cord blood and adult mobilized blood grafts

World Journal of Stem Cells 2011年第11期3卷 104-112页

作者： Nesrine Ben Azouna Faouzi Jenhani Lamia Berraeis Zohra Regaya Immunology Research Unit Faculty of Pharmacy of Monastir Monsatir University TU-5000 Monastir Tunisia Cell Im-munology Cytometry and Cell Therapy Laboratories (National Tunisian Blood Center) BP 294 EL Manar II Tunis 2092 Tunisia

AIM:To study the immunophenotype of hematopoietic progenitor cells from cord blood (CB) grafts (n = 39) in comparison with adult apheresis grafts (AG, n = 229) and pre-apheresis peripheral blood (PAPB) samples (n = 908) using flow cytometry analysis.METHODS: First, we performed a qualitative analysis of CD34+ cell sub-populations in both CB and PAPB grafts using the standardized ISHAGE protocol and a wide panel of 20 monoclonal antibodies. Next, we stud-ied some parameters, such as the age of mothers and the weight of newborns, which can influence the qual-ity and the quantity of CD34+ cells from CB. RESULTS: We found that the percentage of apoptotic cells was high in CB in comparison to PAPB (PAPB: 4.6% ± 2.6% vs CB: 53.4% ± 5.2%, P < 0.001). In CB, the weight of newborn and the age of the mother have the influence on CD34+ cells. The follow-up of Ag CD133in the ISHAGE double platform protocol in association with CD45, CD34 and the 7’AAD shows an equal rate between the two cell populations CD133+CD45+CD34+ high and CD34+CD45+ high with a higher percentage. So, is the inclusion of Ac CD133 necessary in the pres-ent panel included in the ISHAGE methodflLast part, we showed a signif icant presence of interferon γ in CB in comparison to PAPB, the annexin showing the high number of apoptotic cells in CB. CONCLUSION: This study demonstrates that many different obstetric factors must be taken into account when processing and cryo-banking umbilical CB units for transplantation.

关键词： Immunophenotyping Hematopoietic pro- genitor Cord blood mobilized blood

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基于推广SMP准则的边坡稳定性分析

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清华大学学报（自然科学版） 2012年第2期52卷 150-154页

作者：赵衡宋二祥徐明清华大学土木工程系土木工程安全与耐久教育部重点实验室北京100084

相比土工问题中通常采用的Mohr-Coulomb(MC)准则,推广的SMP(spatially mobilized plane)准则考虑了中主应力对土体强度的影响,该文比较了基于上述两准则计算得到的边坡稳定性差异。首先,通过求解三次方程将推广的SMP准则转化为应力点到... 详细信息

相比土工问题中通常采用的Mohr-Coulomb(MC)准则,推广的SMP(spatially mobilized plane)准则考虑了中主应力对土体强度的影响,该文比较了基于上述两准则计算得到的边坡稳定性差异。首先,通过求解三次方程将推广的SMP准则转化为应力点到等倾轴的距离ρ和应力张量第一不变量I1的线性函数,从理论上推导了平面应变条件下推广的SMP准则与MC准则之间的等效关系。然后,推导了非关联流动法则条件下基于推广的SMP准则的理想弹塑性模型的向后Euler本构积分算法,并将其编入FLAC有限差分软件,通过边坡稳定性分析的数值计算验证了理论推导结果:在平面应变条件下采用非关联流动法则时,推广的SMP准则等效于土体强度参数提高15%后的MC准则,即基于推广的SMP准则的边坡安全系数比MC准则高15%。

关键词：推广的SMP(spatially mobilized plane)准则中主应力的影响 Mohr-Coulomb准则边坡稳定性

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Physiological Mechanism of Thermotolerance in Wheat (<i>Triticum aestivum</i>Lin.) Seedlings

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American Journal of Plant Sciences 2018年第13期9卷 2719-2727页

作者： Soyema Khatun Jalal Uddin Ahmed Md. Mahi Imam Mollah Kim Taewan Crop Physiology Division Bangladesh Institute of Nuclear Agriculture Mymensing Bangladesh Department of Crop Botany Bangabandhu Sheikh Mujibur Rahman Agricultural University Gazipur Bangladesh Department of Entomology Patuakhali Science and Technology University Patuakhali Bangladesh Department of Food Science and Biotechnology Andong National University Andong South Korea

A research work was performed under sub-tropical condition (24°8'N, 90°0'E) at Bangabandhu Sheikh Mujibur Rahman Agricultural University with three wheat genotypes (BARI Gom 25, BARI Gom 26 and Pavon 76) to observe the mobilization of seed reserve and seedling growth under normal (15°C/25°C) and elevated (25°C/35°C) temperature in growth chamber. The effect of high temperature on wheat seedling was observed in terms of mobilized seed reserve, respiration and transpiration efficiency and seedling growth. At 35°C temperature, maximum mobilization of seed reserve was observed in BARI Gom varieties (BARI Gom 25 and 26) but the respiration efficiency was higher in Pavon 76 in comparison to other genotypes. Maximum loss of respiration and transpiration at 35°C temperature was found to produce low seedling growth in Pavon 76. It appeared from the result that at high temperature the maximum mobilized seed reserve and subsequently minimum loss of respiration and transpiration collectively contributed a positive role for better seedling growth in BARI Gom 25 and BARI Gom 26. Therefore the better mobilized seed reserve and subsequently minimum loss of respiration and transpiration during seedling development are indicators of the thermo tolerance in growing wheat seedling.

关键词： Thermo Tolerance mobilized Seed Reserve Heat Stress Wheat

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Novel Soil Strength Criterion Compared with Conventional Criteria

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Geomaterials 2017年第1期7卷 25-39页

作者： Shuai Shao Shengjun Shao Yu Zhang Changlu Chen Institute of Geotechnical Engineering Xi’an University of Technology Xi’an China Shaanxi Key Laboratory of Loess Mechanics and Engineering Xi’an China

A novel soil strength criterion is proposed based on the shear stress ratio on a new spatially mobilized plane, where the cube root of principal stresses is constant. The strength failure surface depicted in the principal stress space by this criterion was smoothly conical, with a curved triangle shape on the octahedral plane. A comparative analysis of the strength failure surfaces of the Mohr-Coulomb (M-C), the Drucker-Prager (D-P), the Matsuoka-Nakai (M-N), the Lade-Duncan (L-D), the new criteria, and the shear strength laws of different criteria with parameter b on the π plane showed that the L-D criterion and the new spatially mobilized plane strength criterion were comparable, which revealed the physical essence of the L-D criterion. Comparing the new strength criterion with the measured results of true triaxial tests of 4 kinds of intact loess under conditions of consolidation and drain, the strength law of loess could be described by the new strength criterion under complex stress conditions, and the rationality and reliability of the strength criterion were verified by the correspondence between the criterion and experimental values.

关键词： Spatially mobilized Plane Physical Essence of L-D Criterion Strength of Loess True Triaxial Test

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原岩应力对圆形硐室围岩塑性区的影响分析

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煤矿安全 2017年第9期48卷 204-207页

作者：叶金胜经来旺朱玉飞陈思羽周建安徽理工大学土木建筑学院安徽淮南232001 安徽理工大学力学与光电物理学院安徽淮南232001

为了探究原岩应力对塑性区应力的影响,采用SMP(Spatially mobilized Plane)屈服准则对轴对称的圆形硐室进行理论分析,利用硐室周边和硐室围岩弹塑性交界处的应力边界条件,分别得到了塑性区的应力和硐室的支护力与原岩应力的关系式;研究... 详细信息

为了探究原岩应力对塑性区应力的影响,采用SMP(Spatially mobilized Plane)屈服准则对轴对称的圆形硐室进行理论分析,利用硐室周边和硐室围岩弹塑性交界处的应力边界条件,分别得到了塑性区的应力和硐室的支护力与原岩应力的关系式;研究表明,硐室围岩塑性区的应力和硐室支护力不仅仅与围岩的力学性质有关而且还与原岩应力有关。通过算例分析了原岩应力对塑性区的应力和硐室支护力的影响,分析结果表明:塑性区的应力会随着原岩应力的增大而增大,并且在不同深度处,沿着硐室围岩深部逐渐增大,并且塑性区的应力和支护力均与原岩应力存在明显的线性关系。

关键词： SMP(Spatially mobilized Plane)屈服准则原岩应力圆形硐室塑性区应力支护力

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