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Process-Functional Model of Transportation Mix Concrete

Journal of Transportation Technologies 2014年第2期4卷 157-163页

作者： Andrey Vladimirovich Ostroukh Bashmakov Igor Aleksandrovich Polgun Mikhail Borisovich Department of Automated Control Systems Moscow Automobile & Road Construction State Technical University Moscow Russia Department of Management Moscow Automobile & Road Construction State Technical University Moscow Russia

The paper proposes a process-functional model of transportation mix concrete, which is a structured description of a means of transportation technology mix concrete road at the level of the production process. Range of activities related to the transportation of concrete mixtures is presented in the form of hierarchically nested processes that are coordinated on the basis of general systems theory. The model is described in a strict sequence: process chain→process step→process link, and all built technological chains consist of indivisible units.

关键词： Road Transport Process Chain The Transport Process Transportation of Concrete mixes

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Total RNA Degradation <i>in Vitro</i>and <i>in Vitro</i>by Glutamate Dehydrogenase-Synthesized RNA Enzyme: Biotechnological Applications

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Advances in Bioscience and Biotechnology 2019年第4期10卷 59-85页

作者： Godson O. Osuji Wenceslaus C. Madu Paul M. Johnson College of Agriculture and Human Sciences Prairie View A&M University Prairie View TX USA Imo State Polytechnic Owerri Nigeria

Glutamate dehydrogenase regulates crop development, growth, and biomass yield through its synthesis of non-genetic code-based RNA. Understanding the mechanism of GDH-synthesized RNA enzyme would enhance the agriculture innovation capacity of the more than a billion urban gardeners, smallholder, and limited resources indigenous farmers. Different metabolic variants were prepared by treating peanut growing on healthy soil with stoichiometric mixes of mineral salt solutions. Peanut GDH charge isomers were purified to homogeneity by electrophoresis, and made to synthesize RNA enzyme. Peanut total RNA was 5’-end labeled with [γ-32P]ATP and made to react as substrate in vitro with GDH-synthesized RNA from another metabolic variant of peanut. Agarose, and polyacrylamide gel electrophoresis of the reaction products showed that tRNA, rRNA, and most of the mRNAs were degraded to mononucleotides, but total RNAs that were not mixed with GDH-synthesized RNAs were not degraded. When the non-homologous sequence sections of the GDH-synthesized RNA were clipped out, the homologous sections failed to produce Northern bands with peanut total RNA. Therefore, the non-homologous sequence sections served to identify, position, and align the GDH-synthesized RNA to its target total RNA site independent of genetic code;the degradation of total RNA being via non-canonical base alignments in the enzyme-substrate complex, followed by electromagnetic destruction of the total RNA, the less stable of the two kinds of RNA. This is the science-based corner stone that buttresses the crop production efforts of limited resources farmers because GDH-synthesized RNAs quickly degrade superfluous total RNA of the crop in response to the soil mineral nutrient deficiencies thereby minimizing wastage of metabolic energy in the synthesis of unnecessary protein enzymes while optimizing biomass metabolism, crop growth, and maximum crop yields. In vitro hydrolysis of total RNA by GDH-synthesized RNA is the game c

关键词： Arachis hypogaea Limited Resource Farmers Stoichiometric Salt mixes GDH Isoenzyme Purification Nongenetic Code-Based RNA Electrophoresis DNA:RNA Hybrids

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