检索结果-南通市图书馆

18β-glycyrrhetinic acid regulates mitochondrial ribosomal protein l35-associated apoptosis signaling pathways to inhibit proliferation of gastric carcinoma cells

引用

World Journal of Gastroenterology 2022年第22期28卷 2437-2456页

作者： ling Yuan Yi Yang Xia li Xin Zhou Yu-Hua Du Wen-Jing liu lei Zhang lei Yu Ting-Ting Ma Jia-Xin li Yan Chen Yi Nan College of Pharmacy Ningxia Medical UniversityYinchuan 750004Ningxia Hui Autonomous RegionChina Traditional Chinese Medicine College Ningxia Medical UniversityYinchuan 750004Ningxia Hui Autonomous RegionChina Key laboratory of Hui Ethnic Medicine Modernization of Ministry of Education Ningxia Medical UniversityYinchuan 750004Ningxia Hui Autonomous RegionChina Department of Infectious Diseases The Fourth Hospital of Harbin Medical UniversityHarbin 150001Heilongjiang ProvinceChina

BACKGROUND Gastric carcinoma(GC)is a common gastrointestinal malignancy worldwide.Based on the cancer-related mortality,the current prevention and treatment strategies for GC still show poor clinical results.Therefore,it is important to find effective drug treatment targets.AIM To explore the mechanism by which 18β-glycyrrhetinic acid(18β-GRA)regulates mitochondrial ribosomal protein l35(MRPl35)related signal proteins to inhibit the proliferation of GC cells.METHODS Cell counting kit-8 assay was used to detect the effects of 18β-GRA on the survival rate of human normal gastric mucosal cell line GES-1 and the proliferation of GC cell lines MGC80-3 and BGC-823.The apoptosis and cell cycle were assessed by flow cytometry.Cell invasion and migration were evaluated by Transwell assay,and cell scratch test was used to detect cell migration.Furthermore,a tumor model was established by hypodermic injection of 2.5×106 BGC-823 cells at the selected positions of BAlB/c nude mice to determine the effect of 18β-GRA on GC cell proliferation,and quantitative reverse transcription-polymerase chain reaction(qRT-PCR)was used to detect MRPl35 expression in the engrafted tumors in mice.We used the term tandem mass tag(TMT)labeling combined with liquid chromatography–tandem mass spectrometry to screen for differentially expressed proteins(DEPs)extracted from GC cells and control cells after 18β-GRA intervention.A detailed bioinformatics analysis of these DEPs was performed,including Gene Ontology annotation and enrichment analysis,Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis,and so on.Moreover,STRING database(https://***/)was used to predict proteinprotein interaction(PPI)relationships and Western blot was used to detect the expression of proteins of interest in GC cells.RESUlTS The results indicated that 18β-GRA could inhibit the proliferation of GC cells in a dose-and timedependent manner.It could induce GC cell apoptosis and arrest the cell cycle at G0/G1 phase.The proportion of cells arrested at S phase dec

关键词： Gastric carcinoma 18β-glycyrrhetinic acid mitochondrial ribosomal protein l35 Proliferation Invasion Apoptosis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

MRPl35 is upregulated in colorectal cancer and regulates colorectal cancer cell growth and apoptosis

MRPL35 is upregulated in colorectal cancer and regulates col...

引用

中国生物化学与分子生物学会第十二届全国会员代表大会暨2...

作者： litao Zhang Peifen lu lihong Yan lijun Yang Yutao Wang Junjun Chen Jie Dai Yahui li Zhiming Kang Tao Yang Department of Biochemistry & Molecular Biology Shanxi Medical University Department of Pharmacology Shanxi Medical University

mitochondrial ribosome proteins(MRPs),which are encoded by the nuclear genomic DNA,are important for mitochondrialencoded protein synthesis and mitochondrial function.Emerging evidence suggests that several MRPs also exhibit important extra-mitochondrial functions,such as involvement in apoptosis,protein biosynthesis and signal transduction.In this study,we demonstrate a significant role of mitochondrial ribosomal protein l35 in colorectal cancer(CRC).The expression of MRPl35 was higher in CRC tissues than in matched cancer-adjacent tissues and higher in CRC cells than in normal mucosal epithelial cells.Higher MRPl35 expression in CRC tissue correlated with shorter overall survival for CRC patients.Downregulation of MRPl35 led to increased production of reactive oxygen species(ROS) together with DNA damage,loss of cell proliferation,G2/M arrest,a decrease in mitochondrial membrane potential,apoptosis and autophagy induction.MRPl35 knockdown inhibited tumor proliferation in a CRC xenograft nude mouse model.Furthermore,overexpression of MRPl35 or treatment of cells with the ROS scavenger,N-acetyl cysteine(NAC),abrogated ROS production,cell cycle arrest and apoptosis.These findings suggest that MRPl35 plays an essential role in the development of CRC and may be a potential therapeutic target for CRC.

关键词： mitochondrial ribosomal protein l35 colorectal cancer proliferation apoptosis

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：