检索结果-南通市图书馆

Prohibitin 1 inhibits cell proliferation and induces apoptosis via the p53-mediated mitochondrial pathway in vitro

World Journal of Gastrointestinal Oncology 2024年第2期16卷 398-413页

作者： Juan-Juan Shi Yi-Kai Wang Mu-Qi Wang Jiang Deng Ning Gao Mei Li Ya-Ping Li Xin Zhang Xiao-Li Jia Xiong-Tao Liu Shuang-Suo Dang Wen-Jun Wang Department of Infectious Diseases The Second Affiliated Hospital of Xi’an Jiaotong UniversityXi'an 710004Shaanxi ProvinceChina Department of Operating Room The Second Affiliated Hospital of Xi’an Jiaotong UniversityXi'an 710004Shaanxi ProvinceChina

BACKGROUND Prohibitin 1(PHB1)has been identified as an antiproliferative protein that is highly conserved and ubiquitously expressed,and it participates in a variety of essential cellular functions,including apoptosis,cell cycle regulation,prolifera-tion,and *** evidence indicates that PHB1 may play an important role in the progression of hepatocellular carcinoma(HCC).However,the role of PHB1 in HCC is *** To investigate the effects of PHB1 on the proliferation and apoptosis of human HCC cells and the relevant mechanisms in *** HCC patients and healthy individuals were enrolled in this study according to the inclusion and exclusion criteria;then,PHB1 levels in the sera and liver tissues of these participates were determined using ELISA,RT-PCR,and *** HepG2 and SMMC-7721 cells were transfected with the pEGFP-PHB1 plasmid and PHB1-specific shRNA(shRNA-PHB1)for 24-72 *** prolif-eration was analysed with an MTT *** cycle progression and apoptosis were analysed using flow cytometry(FACS).The mRNA and protein expression levels of the cell cycle-related molecules p21,Cyclin A2,Cyclin E1,and CDK2 and the cell apoptosis-related molecules cytochrome C(Cyt C),p53,Bcl-2,Bax,caspase 3,and caspase 9 were measured by real-time PCR and Western blot,*** Decreased levels of PHB1 were found in the sera and liver tissues of HCC patients compared to those of healthy individuals,and decreased PHB1 was positively correlated with low differentiation,TNM stage III-IV,and alpha-fetoprotein≥400μg/*** of PHB1 significantly inhibited human HCC cell proliferation in a time-dependent *** revealed that the overexpression of PHB1 arrested HCC cells in the G0/G1 phase of the cell cycle and induced *** proportion of cells in the G0/G1 phase was significantly increased and the proportion of cells in the S phase was decreased in HepG2 cells that were transfected with pEGFP-PHB1 compar

关键词： Prohibitin 1 Hepatocellular carcinoma cells Apoptosis Cell cycle mitochondrial pathway

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Euphorbia factor L2 induces apoptosis in A549 cells through the mitochondrial pathway

引用

Acta Pharmaceutica Sinica B 2017年第1期7卷 59-64页

作者： Minting Lin Sili Tang Chao Zhang Hubiao Chen Wenjing Huang Yun Liu Jianye Zhang School of Pharmaceutical Sciences Guangzhou Medical University School of Chinese Medicine Hong Kong Baptist University

Euphorbia factor L2, a lathyrane diterpenoid isolated from caper euphorbia seed(the seeds of Euphorbia lathyris L.), has been traditionally applied to treat cancer. This article focuses on the cytotoxic activity of Euphorbia factor L2 against lung carcinoma A549 cells and the mechanism by which apoptosis is induced. We analyzed the cytotoxicity and related mechanism of Euphorbia factor L2 with an MTT assay, an annexin V-FITC/PI test, a colorimetric assay, and immunoblotting. Euphorbia factor L2 showed potent cytotoxicity to A549 cells. Euphorbia factor L2 led to an increase in reactive oxygen species(ROS) generation,a loss of mitochondrial electrochemical potential, release of cytochrome c, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase, suggesting that Euphorbia factor L2 induced apoptosis through a mitochondrial pathway. The cytotoxic activity of Euphorbia factor L2 in A549 cells and the related mechanisms of apoptotic induction provide support for the further investigation of caper euphorbia seeds.

关键词： Euphorbia Factor L2 Caper euphorbia seed Euphorbia lathyris L Anticancer agent Apoptosis mitochondrial pathway

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Small interfering RNA targeting HMGN5 induces apoptosis via modulation of a mitochondrial pathway and Bcl-2 family proteins in prostate cancer cells

引用

Asian Journal of Andrology 2012年第3期14卷 487-492,I0009页

作者： Xiao-Yu Zhang Zhong-Qiang Guo Shi-Qi Ji Min Zhang Ning Jiang Xue-Song Li Li-Qun Zhou Department of Urology Peking University First Hospital and the Institute of UrologyPeking UniversityBeijing 100034China

We investigated the importance of HMGN5, a nuclear protein that binds to nucleosomes, unfolds chromatin, and affects transcription, in the LNCaP prostate cancer cell line. We also examined the molecular mechanisms that promote apoptosis of LNCaP cells after infection with small interfering RNA （siRNA） targeting HMGN5 （siRNA-HMGN5）. The androgen-dependent LNCaP human prostate cancer cells were infected with siRNA-HMGN5. Apoptosis was detected using the Annexin V-PE/7-AAD double staining and the terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling （TUNEL） assays. mitochondrial membrane potential was measured by JC-1 staining. HMGN5and GAPDHmRNA expression were determined using real-time PCR. Bcl-2 and other apoptosis-related protein levels were determined by Western blot analysis. Caspase activity was measured by cleavage of the caspase substrate. Infection with siRNA targeting HMGN5 efficiently and specifically reduced the HMGN5 expression in LNCaP cells. The downregulation of HMGN5 induced remarkable apoptosis of LNCaP cells and resulted in the reduction of mitochondrial membrane potential. The induction of cell apoptosis was accompanied by the upregulation of Bax, the Bax/Bcl-2 ratio and the activation of caspase3. The HMGN5-targeted siRNA was effective in downregulating the expression of HMGN5 in androgen-dependent prostate cancer cells and inducing cell apoptosis via the regulation of a caspase-related mitochondrial pathway and Bcl-2 family proteins. This study suggests that HMGN5 may be a potential molecular target with therapeutic relevance for the treatment of prostate cancer.

关键词： apoptosis HMGN5 mitochondrial pathway prostate cancer siRNA

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Radix Kansui Stir-Fried with Vinegar Reduces Radix Kansui-Related Hepatotoxicity in Mice via mitochondrial pathway

引用

Chinese Journal of Integrative Medicine 2021年第3期27卷 192-197页

作者： BAO Bei-hua YAN Xiao-jing CAO Yu-dan YAO Wei-feng CHENG Fang-fang CHEN Pei-dong SHAN Ming-qiu ZHANG Li DING An-wei Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative MedicineNanjing University of Chinese MedicineNanjing 210023China Menghe Medical Institute Changzhou Traditional Chinese Medical Hospital Affiliated to Nanjing University of Chinese MedicineChangzhouJiangsu Province 213003China

Objective To investigate the mechanism of Radix Kansui(RK)stir-fried with vinegar(VRK)decreased hepatotoxicity in *** According to a random number table,40 mice were randomly divided into negative control group(0.5%carboxymethylcellulose sodium,20 mL/kg),positive control group(0.1%mixture of carbon tetrachloride in soybean oil,20 mL/kg),RK group(the ethyl acetate extracts of RK,250 g crude drug/kg)and VRK group(the ethyl acetate extracts of VRK,250 g crude drug/kg)with 10 mice per *** mice were administered orally by gavage daily for 7 continuous *** morphology of liver tissues was examined to assess the liver injury by a transmission electron *** apoptosis in vivo was determined by terminal deoxynucleotidyltransferase-mediated dUTP-biotin nickend labeling(TUNEL)*** technique was adopted to detect the expression of particular antiapoptotic and proapoptotic proteins in the mitochondrial pathways,including B-cell lymphoma(Bcl-2)and caspase-3,as well as the expression of inflammatory mediators,including nuclear factor kappa B(NF-κB)and intercellular adhesion molecule-1(ICAM-1).Results Liver injury and hepatocyte apoptosis were observed in RK mice,and the liver injury were significantly reduced in VRK-treated *** immunohistochemistry study,compared with the negative control group,RK inhibited dramatically the Bcl-2 protein expression and significantly increased the expression of caspase-3,NF-κB and ICAM-1(all Pmitochondrial pathway.

关键词： Radix Kansui mitochondrial pathway hepatotoxicity apoptosis inflammation Chinese medicine

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Molecular mechanisms of apoptosis in hepatocellular carcinoma cells induced by ethanol extracts of Solanum lyratum Thumb through the mitochondrial pathway

引用

World Journal of Gastroenterology 2017年第6期23卷 1010-1017页

作者： Xiao-Qiang Mo Hong-Yu Wei Gan-Rong Huang Ling-Yuan Xu Yu-Li Chen Jiang Qi Wei Xian Yan-Chun Qin Lian-Deng Wei Li-Juan Zhao Yan-Qiang Huang Wei Xing Hong-Qin Pu Peng-Ya Wei Chao-Gan Li Qiu-Chun Liang Breeding Base of Microbial Infection Key Laboratory in the West Guangxi Youjiang Medical University for Nationalities Affiliated Hospital of Youjiang Medical University for Nationalities

AIM To explore the induction effects and mechanism of Solanum lyratum Thumb(ST) on human hepatocellularcarcinoma SMMC-7721 cells through the mitochondrial *** The experiments were conducted on three groups: an experimental group (with ST ethanol extracts' concentration being 2.5, 5 and 10 mg/L), a negative control group (with only nutrient solution, 0 mg/L ST ethanol extracts), and a positive control group (2.5 mg/L DDP). The inhibition rate of cell proliferation was checked by using the methyl thiazolyl tetrazolium method, and cell apoptosis was tested by TUNEL method. Furthermore, RT-PCR was used to examine m RNA expression of Fas, Fas L, caspase-8, caspase-3, p53 and Bcl-2 *** Compared with the negative control group, the inhibition and apoptosis rates of the experimental group with different concentrations of ST extracts on human hepatocellular carcinoma SMMC-7721 cells significantly increased(Pmitochondrial pathway.

关键词： Solanum lyratum Thumb hepatocellular carcinoma cell cell apoptosis mitochondrial pathway molecular mechanism

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

mitochondrial pathway in Cadmium-Induced Apoptosis of Rat's Primary Cultured Cerebral Cortical Neurons

Mitochondrial Pathway in Cadmium-Induced Apoptosis of Rat's ...

引用

第三届中国兽医临床大会

作者： YUAN Yan,JIANG Chen-yang,SUN Ya,BIAN Jian-chun,LIU Xue-zhong,LIU Zong-ping~* College of Veterinary Medicine,Yang Zhou University,Yangzhou 225009,China

Background and aims Cadmium(Cd)is an environmental pollutant which is widely used in industry and is a constituent of tobacco *** are in vitro studies showing the neurotoxicity of Cd at uM range on cell culture models like neurons and glial cells,and the Cd-mediated toxicity is thought to involve,at least in part,the induction of ***,the mechanisms of Cd-induced apoptosis have not been well ***-induced apoptosis in vivo and in vitro was found to primarily involve the intrinsic mitochondrial-dependent *** the current experiment,mitochondrial pathway in Cd-induced apoptosis of rat’s cerebral cortical neurons was *** The model of rat’s primary cerebral cortical neurons in vitro was established successfully,which were obtained from Sprague-Dawley rat embryos of 18-19 days *** neurons were exposured to cadmium acetate of different concentrations(0,5,10,20μmol/L)for 12 ***,the ultrastructural changes of mitochondria,the morphological changes of apoptosis,the level of reactive oxygen species(ROS),mitochondrial membrane potential(Δψm),the transcriptional level of Caspase-3 mRNA and protein expression of Bcl-2 and Bax were *** addition,the neurons were exposured to 10μmol/L Cd for 0,12,24,48 h,and the cleaved Caspase-9,3 and cleaved PARP were *** After Cd exposure,in comparison with the control group,the results showed that ultrastructural changes were distortion of mitochondrial cristae and unusual arrangements,typical morphological changes of apoptosis were nucleus crimpled and chromatin condensedation, even nucleus disintegratation in Cd-treated *** addition,ROS level and the transcriptional level of Caspase-3 mRNA increased significantly(P<0.05 or P<0.01),in contrast,Δψm decreased significantly(P<0.05 or P<0.01).Meanwhile,the results obtained by western blot indicated that protein expression levels of Bcl-2 decreased and Bax increased with the increasing concentration of Cd exposu

关键词： Cadmium Cerebral cortical neurons Apoptosis mitochondrial pathway

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Daphnetin induces apoptosis in fibroblast-like synoviocytes from collagen-induced arthritic rats mainly via the mitochondrial pathway

Daphnetin induces apoptosis in fibroblast-like synoviocytes ...

引用

第十三届全国免疫学学术大会

作者： Mao Zheng Nanzhen Kuang Xiaoping Zeng Jieying Wang Yu Zou Yingyuan Fu Department of Immunology School of Basic Medical Sciences Nanchang University

We have previously found that daphnetin(DAP) has a pro-apoptotic effect on fibroblast-like synoviocytes(FLSs) from collagen-induced arthritis(CIA) *** the present study, we further investigated the mechanism of DAP-induced apoptosis in ***-FLSs were incubated with DAP for 48 h in the presence or absence of caspase inhibitors, including inhibitors of caspase-3, caspase-8, or caspase-9 or a pan-caspase inhibitor;then, a series of experiments were *** results showed that DAP markedly decreased cell viability and induced the apoptosis of CIAFLSs, along with typical morphological and ultrastructural changes;moreover, DAP increased the mRNA expression of FasL, cytochrome c(Cyt-c), Bax, caspase-3, caspase-8, and caspase-9 and the protein expression of Bax, caspase-3, caspase-8, and *** contrast, DAP decreased the mRNA and protein expression of Bcl-2 and promoted the release of Cyt-c from the mitochondria into the cytosol;these effects were attenuated to varying degrees by pre-treatment with caspase inhibitors, especially with caspase-3 or caspase-9 inhibitors or a pan-caspase *** conclusion, the current findings demonstrate that the DAP-induced apoptosis of CIA-FLSs occurred mainly via a caspase-dependent pathway, in particular the mitochondrial pathway, and that the Bax/Bcl-2 ratio was involved in this ***, DAP may be a potential therapeutic agent for RA.

关键词： Daphnetin CIA-FLS apoptosis mitochondrial pathway

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Up-regulated RBM5 promotes the apoptosis of A172 glioma cells via mitochondrial pathway

Up-regulated RBM5 promotes the apoptosis of A172 glioma cell...

引用

第十三届中国医师协会神经外科医师年会

作者： Haoyang Huang Dong Zhong Shuang Shi Fu'an Zhang Jiong Li Dong Lv Department of Neurosurgery The First Affiliated Hospital of Chongqing Medical University Experimental Research Center The First Affiliated Hospital of Chongqing Medical University

Abbreviation Bax=Bcl-2 Associated Protein;Bcl-2=B-cell Lymphona-2;Cleaved-caspase-9=Cleaved cysteinyl aspartate specific proteinase-9;DAB=Diaminobenzidine;DMEM=Dulbecco’s modified eagle medium;ECL=Electrochemiluminescence;FBS=Fetal bovine serum;EDTA=Ethylene Diamine Tetraacetic Acid;FCM=Flow cytometry;PBS=Phosphate buffer saline;PMSF=Phenylmethanesulfonyl fluoride;PVDF=Polyvinylidene fluoride;qRT-PCR=Quantitative real-time polymerase chain reaction;RBM5=RNA binding motif-5;SDS=Sodium dodecyl sulfate;SYBR=Synergy Brands Synergy Brands;TBST=Tris-HCl ***=Tumor suppressor ***:.Primary brain tumors account for 2% of all malignant neoplasms in adults,and approximately half of them represent gliomas.1 Recent reports indicated that the lack of tumor suppressor genes（TSG）contribute to the ***5,mapping to 3 p21.3,was found lowly expressing or deficient among some cancer tissues,especially the lung caner.2 This study made a hypothesis that RBM5 probably represents decreasing or deficiency in glioma tissues and ***:Preliminarily,qRT-PCR was conducted to detect the expression of RBM5 in human glioma *** glioma tissue specimens（WHO Ⅰ～Ⅳ）obtained from patients with astrocytoma and glioblastoma were collected so as to investigate the positive expression of RBM5 in those tissues via *** lentivirus with up-regulated RBM5 sequence,synthesized chemically in vitro,transfected into A172 *** expression of RBM5 in transfected A172 cells was proved to be elevated through q *** apoptotic effects of A172 cells were analyzed by FCM and Hoechest *** proteins associated with apoptosis were assessed through western ***:In the explorative research,RBM5 was shown that it represented more expression in low-grade glioma tissues（WHO Ⅰ～Ⅱ）than that in high-grade ones（WHO Ⅲ～Ⅳ）.The tendency of RBM5 expressing existed in glioma cell ***-PCR proved the A172 cell line was successfully transfected with lentivirus

关键词： RBM5 glioma apoptosis mitochondrial pathway

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Endothelial-monocyte-activating polypeptide Ⅱ induces rat C6 glioma cell apoptosis through mitochondrial pathway

Endothelial-monocyte-activating polypeptide Ⅱ induces rat C...

引用

中国神经科学学会第十届全国学术会议

作者： Li-Bo LIU Hui XIE Yi-Xue XUE Yun-Hui LIU Zhen LI Ping WANG Department of Neurobiology College of Basic MedicineChina Medical University Department of Neurosurgery Shengjing HospitalChina Medical University

Objective Endothehal-monocyte-activating polypeptide Ⅱ（EMAP Ⅱ） has been shown to inhibit rumors by targeting vascular ***,the effect of EMAP Ⅱ on glioma cell of treating brain glioma has not been precisely *** present study was performed to examine whether EMAP Ⅱ inhibit glioma growth by inducing rat brain glioma C6 cells *** The viability of rat C6 glioma cells was detected by MTT *** Nuclear characteristic changes of apoptosis were visualized by Hoechst DNA *** cytometry was used to analyze the apoptotic proportion and the change of mitochondrial membrane potential（MMP） of C6 glioma *** activity of caspase-3 and caspase-9 were tested by respective activity assay *** blot assay was used to test the protein expression of caspase-3,caspase-9,reactive oxygen species（ROS）,Bcl-2,Bax and *** En rat C6 glioma cells treated with different EMAP-EI for different time,compared with EMAP-Ⅱ 0 h group,EMAP-EE could inhibit the viability（≥0.05 nM）.Meanwhile,apoptotic proportion increased gradually in a time-dependent manner and displayed typical changes,including bright stained and condensed or fragmented *** Ⅱ induced a rapid decrease in MMP and the release of cytochrome c into the cytosol,followed by activation of caspase-9 and *** the same time,EMAP El-induced apoptosis was accompanied by an increase of ROS *** significant up-regulation in the expressions of Bax and Apaf-1 as well as down-regulation in the expression of Bcl-2 was observed after EMAP Ⅱ *** time course change of ROS was prior to the changes of above investigated indexes that were associated with mitochondrial-mediated apoptotic *** In summary,our present study suggests that a ROS-mediated mitochondrial pathway played an important role in the process of EMAP Ⅱ-induced apoptosis of rat brain glioma C6 cells.

关键词： endothelial monocyte-activating polypeptide-ii apoptosis glioma mitochondrial pathway

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Daphnetin induces apoptosis in fibroblast-like synoviocytes from collagen-induced arthritic rats mainly via the mitochondrial pathway

Daphnetin induces apoptosis in fibroblast-like synoviocytes ...

引用

第十一届全国免疫学学术大会

作者： Zheng Mao Kuang Nanzhen Zeng Xiaoping Wang Jieying Fu Yingyuan Nanchang University

We have previously found that daphnetin(DAP) has a pro-apoptotic effect on fibroblast-like synoviocytes(FLSs) from collagen-induced arthritis(CIA) rats. In the present study, we further investigated the mechanism of DAP-induced apoptosis in CIA-FLSs. CIA-FLSs were incubated with DAP for 48 h in the presence or absence of caspase inhibitors, including inhibitors of caspase-3, caspase-8, or caspase-9 or a pan-caspase inhibitor;then, a series of experiments were performed to evaluate the mechanisms of DAP-induced apoptosis. Our results showed that DAP markedly decreased cell viability and induced the apoptosis of CIA-FLSs, along with typical morphological and ultrastructural changes;moreover, DAP increased the mRNA expression of Fas L, cytochrome c(Cyt-c), Bax, caspase-3, caspase-8, and caspase-9 and the protein expression of Bax, caspase-3, caspase-8, and caspase-9. In contrast, DAP decreased the mRNA and protein expression of Bcl-2 and promoted the release of Cyt-c from the mitochondria into the cytosol;these effects were attenuated to varying degrees by pre-treatment with caspase inhibitors, especially with caspase-3 or caspase-9 inhibitors or a pan-caspase inhibitor. In conclusion, the current findings demonstrate that the DAP-induced apoptosis of CIA-FLSs occurred mainly via a caspase-dependent pathway, in particular the mitochondrial pathway, and that the Bax/Bcl-2 ratio was involved in this process. Thus, DAP may be a potential therapeutic agent for RA.

关键词： daphnetin FLSs apoptosis mitochondrial pathway

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：