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Effects of heavy metals Cd^(2+),Pb^(2+)and Zn^(2+)on DNA damage of loach misgurnus anguillicaudatus

Frontiers in Biology 2008年第1期3卷 50-54页

作者： Yingmei ZHANG Yejing WANG Runliu YU Sheng ZHANG Zhenbin WU School of Life Sciences Lanzhou UniversityLanzhou 730000China State Key Laboratory of Freshwater Ecology and Biotechnology Institute of Hydrobiologythe Chinese Academy of ScienceWuhan 430072China

The effects of heavy metals Cd^(2+),Pb^(2+)and Zn^(2+)at 0.05,0.5 and 5.0 mg/L level and their interactions at 0.5 mg/L level on DNA damage in hepatopancreas of loach misgurnus anguillicaudatus for 1-35 days exposure were examined by single cell gel electrophoresis(SCGE).For each test group,20 loaches with similar body size(5.17-7.99 g;11.79-13.21 cm)were selected and kept in aquaria with dechlori-nated water at(22±1)℃and fed a commercial diet every 48 *** to the percentage of damaged DNA with tail and its TL/D(tail length to diameter of nucleus)value,the relationship between DNA damage degree and heavy metal dose and exposure time was *** showed that the percentage of damaged DNA and the TL/D value were increased with the prolonged exposure *** highest percentage(84.85%)of damaged DNA was shown in 5.0 mg/L Zn^(2+)group after 28 days exposure and the biggest TL/D value(2.50)in all treated groups after 35 days *** the first treated week,the damnification of DNA was mainly recognized as the first level,after that time,the third damaged level was mostly observed and the percentage of damaged DNA was beyond 80%.The joint toxic effects among Cd^(2+),Pb^(2+)or Zn^(2+)revealed much complexity,but it generally displayed that the presence of Cd^(2+)could enhance the genotoxicity of Pb^(2+)or Zn^(2+).In conclusion,the results suggestedthattherewasasignificanttime-anddose-depended relationship between the heavy metal and DNA damage in hepatopancreas of loach,and SCGE could represent a useful means to evaluate the genotoxicity of environmental contamination on aquatic organisms.

关键词： misgurnus anguillicaudatus single cell gel electrophoresis(SCGE) heavy metals DNA damage

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The effects of two free-floating plants (Eichhornia crassipes and Pistia stratiotes) on the burrow morphology and water quality characteristics of pond loach (misgurnus anguillicaudatus) habitat

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Aquaculture and Fisheries 2018年第1期3卷 22-29页

作者： Jinqing Wang Guihua Fu Weiyue Li Ying Shi Jicai Pang Qiang Wang Weiguang Lü Change Liu Jiansheng Liu Department of Animal Nutrition and Fisheries Shandong Vocational Animal Science and Veterinary CollegeWeifang 261061China Eco-environmental Protection Institute of Shanghai Academy of Agricultural Sciences Shanghai 201403China Yangzhou City Yishan Agriculture Technology Co. LtdYangzhou 225624China

Loach exhibit conspicuous drilling behaviors in the mud of shallow waters,yet their burrow morphology and the factors affecting this behavior have received little *** characterized the burrow morphology and water quality of the pond loach misgurnus anguillicaudatus in three scenarios:in tanks without plants,tanks with the free-floating plant water hyacinth Eichhornia crassipes,and tanks with water lettuce Pistia *** hyacinth effectively removed water TN,COD,NO3-N and NH4-N,and water lettuce removed water TP and *** hyacinth and water lettuce markedly reduced water turbidity and DO,increased TOC and *** hyacinth purified water more effectively than water lettuce,providing a suitable habitat for loach feeding,living and *** burrow structures were V-shaped,Y-shaped,inverted L-shaped,or complicated dendritic networks composed of multiple V *** hyacinth treatment was characterized by the greatest burrow volume,length,depth,and structural complexity,but the opening size was reduced by dense root mat *** in the water lettuce treatment were characterized by intermediate volume,length,branches and sinuosity,but they had the largest opening and pit *** control treatment had a flat bottom with the smallest,shortest *** study indicates that free-floating plants improve habitat suitability and change burrow morphology and may be used to improve loach breeding methods。

关键词： Freshwater benthic fish misgurnus anguillicaudatus Burrow morphology Free-floating plant Eichhornia crassipes Pistia stratiotes Water purification

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Effect of Fish Meal Replacement with Maggot Meal on the Growth Performance of misgurnus anguillicaudatus

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Agricultural Biotechnology 2016年第6期5卷 24-27页

作者： Haitao ZHANG Yunlong LI Kailang GU Feng GAO Department of Animal Engineering Xuzhou Vocational College of BioengineeringXuzhou 221006China

Maggot meal was used to replace 0, 20% , 40% , 60% , 80% , 100% of the fish meal in the hasal feed, getting 6 kinds of feed with the same nitrogen content and equal energy （marked as group H0, H20, H40, H60, H80, H100）, which were used to feed the misgurnus anguillicaudatus （loach） for60 d. The effects of the fish meal replacement by maggot meal on the growth performance of M. anguiUicaudatus were studied by comparing the growth performances and body indica- tors of M. anguillicaudatus fed with different feed groups. The results showed that the final weight, weight gain rate, specific growth rate of the M. anguillicaudatus in group H40 showed no significant difference with the control P 〉0.05 ）, but was significantly higher than that of other groups P 〈0.05 ）. Moreover, except group H40, the feed coefficients of all other groups were significantly higher than that in control group （P 〈0.05 ）. The M. anguillicaudatus in group 1-140 had the highest condition factor and COR and the lowest viscera index, all of which showed significant differences with the other replacement groups （P 〈0.05） but the difference with the control group was not significant （P 〉 0.05）. Thus, a proportion of 40% of the fish meal replaced with maggot meal in the mixed feed for the M. anguilli- caudatus could improve the growth performance and physique indexes of M. anguillicaudatus.

关键词： misgurnus anguillicaudatus Maggot meal Fish meal Growth performance

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PCR Amplification of Sox Genes in misgurnus anguillicaudatus and Paramisgurnus dabryanus(Cypriniform;Cobitidae)

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Wuhan University Journal of Natural Sciences 1997年第2期2卷 128-130页

作者： Chang Zhongjie Zhou Rongjia Yu Qixing College of Life Sciences Wuhan University Wuhan 430072China

Using the primers which specially amplify the conservative motif of Human SRYgene,we stud ied the PCR amplification of Sox genes in genomic DNA of two species of mud loach Misgurus anguillicau-datus and Paramisgurnus *** bands with the length of 200,550,940 and 1000 bp respectively,were presented in the PCR products of Misgumus *** bands with the length of 200,550 and 900 bp were presented in that of Paramisgumus *** blotting results indicated that the 200 and 550 bp bands are specially *** is no difference between male and female individuals as well as between these two species.

关键词： Sox gene misgurnus anguillicaudatus Paramisgumus dabryanus

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Isolation and Characterization of an Antimicrobial Polypeptide from Loach

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Chinese Chemical Letters 2003年第1期14卷 54-57页

作者： Xian Zhi DONG Hui Bi XU Kai Xun HUANG Qiong LIU Department of Chemistry Huazhong University of Science and TechnologyWuhan 430074

A novel antimicrobial polypeptide was isolated and characterized from loach, misgurnus anguillicaudatus. The polypeptide, named MAPP, is a single-chain polypeptide with Mw about 9,800 Dalton and pI about 4.78; the N-tag of MAPP was CFGWN. MAPP showed good inhibition against various bacteria including Bacillus subtilis, Escherichia coli and Staphylococcus aureus. MAPP could be used as a lead compound in antibiotics drug discovery.

关键词： misgurnus anguillicaudatus isolation characterization.

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Effects of Zn^(2+) and Cu^(2+) on loach ovaries and ova development

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Zoological Research 2013年第5期34卷 135-139页

作者： Jian-Xun TANG Jun-Rong LI Zhong-Liang LIU Hua ZHAO Xiao-Min TAO Zhang-Shun CHENG College of Agriculture and Bio-engineering Jinhua Polytechnic InstituteJinhua321007China College of Pharmacy and Material Engineering Jinhua Polytechnic InstituteJinhua321007China

This study compared the accumulation of Zn^(2+)and Cu^(2+)in the ovaries and ova of loaches under different concentrations of Zn^(2+)(1.00,2.50 and 5.00 mg/L respectively)and Cu^(2+)(0.10,0.25 and 0.50 mg/L respectively).The results showed that both Zn^(2+)and Cu^(2+)accumulated in the ovaries,and that the relationship between accumulation and time was linear over 20 days of *** accumulation of the metals in ovaries was closely related to the concentration of exposure in the solutions(P<0.05),and was obviously affected by the time and ***,the Cu^(2+)concentration was significantly higher than Zn^(2+)(P<0.05).The development level of ova in the ovaries also correlated with the concentration and exposure period in the Zn^(2+)and Cu^(2+)solutions.

关键词： misgurnus anguillicaudatus Ovary Ovum Zn Cu Accumulation

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Effects of Zn;and Cu;on loach ovaries and ova development

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动物学研究 2013年第5期 135-139页

作者： Jian-Xun TANG Jun-Rong LI Zhong-Liang LIU Hua ZHAO Xiao-Min TAO Zhang-Shun CHENG College of Agriculture and Bio-engineering Jinhua Polytechnic Institute College of Pharmacy and Material Engineering Jinhua Polytechnic Institute

This study compared the accumulation of Zn2+and Cu2+in the ovaries and ova of loaches under different concentrations of Zn2+(1.00, 2.50 and 5.00 mg/L respectively) and Cu2+(0.10, 0.25 and 0.50 mg/L respectively). The results showed that both Zn2+and Cu2+accumulated in the ovaries, and that the relationship between accumulation and time was linear over 20 days of exposure. The accumulation of the metals in ovaries was closely related to the concentration of exposure in the solutions(P<0.05), and was obviously affected by the time and doses. However, the Cu2+concentration was significantly higher than Zn2+(P<0.05). The development level of ova in the ovaries also correlated with the concentration and exposure period in the Zn2+and Cu2+solutions.

关键词： misgurnus anguillicaudatus Ovary Ovum Zn Cu Accumulation

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Microsatellite Analysis of Genetic Diversity Between Loach with Different Levels of Ploidy

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Journal of Northeast Agricultural University(English Edition) 2014年第4期21卷 47-53页

作者： Li Ya-juan Qi Hong-rui Ma Hai-yan Zhou He Xu Wen Sui Yi Li Jia-qi Key Laboratory of Marine Bio-resources Restoration and Habitat Reparation in Liaoning Province Key Laboratory of Mariculture & Stock Enhancement in North China's Sea Ministry of Agriculture Dalian Ocean University

We used microsatellite markers to investigate the genetic parameters of three different polyploidy populations of misgurnus anguillicaudatus from Honghu City, Hubei Province. Polyacrylamide gel electrophoresis banding patterns of diploid（2n）, triploid（3n） and tetraploid loaches（4n） were analyzed with Pop Gen software. A total of 68 alleles were obtained from seven microsatellite loci and the polymorphism information content（PIC） indices were all above 0.5. The average expected mean heterozygosity values（He） were 0.8420, 0.7186 and 0.8521; the average observed mean heterozygosity values（Ho） were 0.9674, 0.9785 and 0.8928; and the HardyWeinberg P values were 0.3078, 0.3151 and 0.3762, for diploid, triploid and tetraploid individuals, respectively. The results indicated that the three populations were highly polymorphic, with no deviations from Hardy-Weinberg equilibrium observed at all the seven microsatellite loci. This indicated a high level of genetic diversity within the populations. A cluster analysis diagram showed that the shortest genetic distance was between diploid and tetraploid loaches and they shared a close phylogenetic relationship. The triploid and tetraploid individuals had the most distant phylogenetic relationship.

关键词： misgurnus anguillicaudatus diploid triploid tetraploid microsatellite genetic diversity

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