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Effect of Same-dose Single or Dual Field Irradiation on Damage to miniature pig Parotid Glands

International Journal of Oral Science 2009年第1期1卷 16-25页

作者： Xing Yan Bo Hai Zhao-chen Shan Chang-yu Zheng Chun-mei Zhang Song-lin Wang Salivary Gland Disease Center and the Molecular Laboratory for Gene Therapy Capital Medical University School ofStomatology Beijing China Beijing Friendship Hospital Capital Medical University Beijing China Gene Therapy and Therapeutics Branch National Institute of Dental and Craniofacial Research NIH DHHS BethesdaMD USA Department of Biochemistry and Molecular Biology Capital Medical University Beijing China

Aim To evaluate the effect of single or dual field irra- diation （IR） with the same dose on damage to miniature pig parotid glands. Methodology Sixteen miniature pigs were divided into two IR groups （n=6） and a control group （n=4）. The irradi- ation groups were subjected to 20 Gy X-radiation to one parotid gland using single-field or dual-field modality by linear accelerator. The dose-volume distributions between two IR groups were compared. Saliva from parotid glands and blood were collected at 0, 4, 8 and 16 weeks after irradiation. Parotid glands were removed at 16 weeks to evaluate tissue morphology.Results The irradiation dose volume distributions were significantly different between single and dual field irradi- ation groups （t----4.177, P=0.002）, although dose volume histogramin （DVH） indicated the equal maximal dose in parotid glands. Saliva flow rates from IR side decreased dramatically at all time points in IR groups, especially in dual field irradiation group. The radiation caused changes of white blood cell count in blood, lactate dehydrogenase and amylase in serum, calcium, potassium and amylase in saliva. Morphologically, more severe radiation damage was found in irradiated parotid glands from dual field irradi- ation group than that from single field irradiation group. Conclusion Data from this large animal model demons- trated that the radiation damage from the dual field irradiation was more severe than that of the single field irradiation at the same dose, suggesting that dose-volume distribution is an important factor in evaluation of the radiobiology of parotid glands.

关键词： irradiation damage miniature pig parotidgland

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Application of Gas Chromatography-mass Spectrometry in Analyzing Pharmacokinetics and Distribution of Deltamethrin in miniature pig Tissues

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Biomedical and Environmental Sciences 2014年第6期27卷 426-435页

作者： ZHU Pan FAN Sai ZOU Jian Hong MIAO Hong LI Jing Guang ZHANG Guo Wen WU Yong Ning State Key Laboratory of Food Science and Technology Nanchang University Key Laboratory of Food Safety Risk Assessment of Ministry of Health China National Center for Food Safety Risk Assessment Institute of Nutrition and Food Safety Beijing Centre of Disease Control and Prevention The Second Artillery General Hospital

Objective To characterize the pharmacokinetics and distribution profiles of deltamethrin in miniature pig tissues by gas chromatography-mass spectrometry （GC-MS）. Methods Pharmacokinetics and distribution of deltamethrin in blood and tissues of 30 miniature pigs were studied by GC-MS after oral administration of deltamethrin （5 mg/kg bw）. Data were processed by 3P97 software. Results The serum deltamethrin level was significantly lower in tissues than in blood of miniature *** AUC0-72 h, Cmax, of deltamethrin were 555.330±316.987 ng h/mL and 17.861±11.129 ng/mL, respectively. The Tmax, of deltamethrin was 6.004±3.131 h.Conclusion The metabolism of deltamethrin in miniature pigs is fit for a one-compartment model with a weighting function of I/C^2. Deltamethrin is rapidly hydrolyzed and accumulated in miniature pig tissues.

关键词： Deltamethrin miniature pig Pharmacokinetics Tissue distribution GC-MS

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MICRODISSECTION OF miniature pig EAR

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Journal of Otology 2013年第2期8卷 91-96页

作者： YI Haijin GUO Weiwei CHEN Lei WU Na LI Jiana REN Lili YANG Shiming Department of Otolaryngology Head and Neck SurgeryInstitute of Otolaryngology Chinese PLA General Hospital Department of Neurosurgery Division Otolaryngology- Head and Neck Surgery Beijing Tiantan Hospital Capital University of Medical Science State Key Laboratory for Agro- Biotechnology China Agricultural University

Objective To investigate the suitability of miniature pigs as an animal model for otological research. Methods Microdissection of the temporal bone was performed on 10 miniature pigs and recorded on photo-graphs. Results The morphology and measurement of the external, middle and inner ear and the lateral re-cess of the miniature pigs were obtained by microdissection. Conclusion Compared to traditional animal models, the miniature pig may be a better model for biomedical research because of its many similarities in physiological functions with humans. Similarities of the temporal bone structures, including the external, middle and inner ear and the lateral recess, between the miniature pig and human make the animal a poten-tially useful model for otological research.

关键词： miniature pig animal model otological research.

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Morphological characteristics of submandibular glands of miniature pig

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Chinese Medical Journal 2005年第16期118卷 1368-1373页

作者： Zhang Xin Li Jun Liu Xiao-yong Sun Yi-lin Zhang Chun-mei Wang Song-ling Salivary Gland Disease Center and Molecular Laboratory for Gene Therapy Faculty of Stomatology Capital University of Medical Sciences Beijing 100050 China Uhrastructure Unit Department of Pathology Chinese Academy Medical Sciences Neuroscience Institute Beijing 100050 China

Background miniature pig （minipig） is increasingly used as a large animal model for a variety of biomedical studies. Little information is available in the literature on anatomy, histology and sialogragh of the submandibular gland of the minipig. The purpose of this study was to characterize the morphology of a miniature pig＇s （minipig） submandibular gland as a large animal model for further biomedical studies. Methods Five minipigs were subjected to sialographic, anatomic, histologic, histochemical and uhrastructural evaluations for submandibular glands. Results Sialograms showed a long, horizontal main excretory duct and a pear-shaped gland located inferoposterior to the angle of the mandible. The submandibular glands lied superficial to the suprahyoid, and infrahyoid muscle groups, and were covered by the inferior portion of the parotid gland. The submandibular glands were characterized by a mixed parenchyma of mucous and serous secretory aeini. Alcian blue （AB） staining and periodic acid-Schiff （PAS） reactions demon-strated that minipig submandibular glands synthesized and secreted acid mucous substances by serous cells and polysaccharide, and neutral mucous substances, by mucous cells. Conclusion The submandibular gland of the minipig is considered a useful large salivary gland animal model for biomedical studies.

关键词： submandibular gland miniature pig sialography anatomy histology

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Preliminary study of metabonomic changes during the progression of atherosclerosis in miniature pigs

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Animal Models and Experimental Medicine 2024年第4期7卷 419-432页

作者： Yunxiao Jia Yuqiong Zhao Miaomiao Niu Changqi Zhao Xuezhuang Li Hua Chen Laboratory Animal Center Chinese PLA General HospitalBeijingPeople's Republic of China

Background:To explore potential biomarkers for early diagnosis of atherosclerosis(AS)and provide basic data for further research on AS,the characteristics of serum metabolomics during the progression of AS in mini-pigs were observed ***:An AS model in Bama miniature pigs was established by a high-cholesterol and high-fat *** serum samples were collected monthly for metabolomics and serum lipid *** the end of the treatment period,pathological analysis of the abdominal aorta and coronary artery was performed to evaluate the lesions of AS,thereby distinguishing the susceptibility of mini-pigs to *** metabolomics was de-tected using a high-resolution untargeted metabolomic *** analysis was used to identify metabolites associated with AS ***:Based on pathological analysis,mini-pigs were divided into two groups:a susceptible group(n=3)and a non-susceptible group(n=6).A total of 1318 metabo-lites were identified,with significant shifting of metabolic profiles over time in both *** monitoring analysis highlighted 57 metabolites that exhibited an ob-vious trend of differential changes between two groups with the advance of *** KEGG(Kyoto Encyclopedia of Genes and Genomes)pathway enrichment analysis in-dicated significant disorders in cholesterol metabolism,primary bile acid metabolism,histidine metabolism,as well as taurine and hypotaurine ***:During the progression of AS in mini-pigs induced by high-cholesterol/high-fat diet,the alterations in serum metabolic profile exhibited a time-dependent pattern,accompanied by notable disturbances in lipid metabolism,cholesterol me-tabolism,and amino acid *** metabolites may become potential bio-markers for early diagnosis of AS.

关键词： atherosclerosis metabolomics miniature pig susceptibility

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Vestibular-evoked myogenic potentials in miniature pigs

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Journal of Otology 2016年第2期11卷 88-93页

作者： Xi Shi Yan Zhang Ya Li Shiwei Qiu Shili Zhang Yaohan Li Na Yuan Yuehua Qiao Shiming Yang Institute of Audiology and Balance Science Xuzhou Medical University Department of Otorhinolaryngology Head and Neck Surgery First Hospital of Jilin University Clinical Hearing Center Affiliated Hospital of Xuzhou Medical University Department of OtolaryngologydHead & Neck Surgery Institute of Otolaryngology of PLA Chinese PLA General Hospital

Objective:To report detection of vestibular-evoked myogenic potentials (VEMPs) in the miniature pig. Methods:Potentials evoked by 1000 Hz tone bursts were recorded from neck extensor muscles and the masseter muscles in normal adult Bama miniature pigs anesthetized with 3%pentobarbital sodium and Carbachol II. Results:The latency of the first positive wave P from neck extensor muscles was 7.65 ± 0.64 ms, with an amplitude of 1.66 ± 0.34 uv and a rate of successful induction of 75%at 80 dB SPL. The latency of potentials evoked from the masseter muscles was 7.60 ± 0.78 ms, with an amplitude of 1.31 ± 0.28 uv and a rate successful induction of 66%at 80 dB SPL. Conclusion:The latencies and thresholds of VEMPs recorded from the neck extensor muscle and the masseter muscle appear to be comparable in normal adult Bama miniature pigs, although the amplitude recorded from the neck extensor muscle seems to be higher than that from the masseter muscle. However, because of their usually relatively superficial and easily accessible location, as well as their large volume and strong contractions, masseter muscles may be better target muscles for recording myogenic potentials.

关键词： Vestibular evoked myogenic potentials miniature pig Masseter myogenic potential

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Application of sodium alginate microspheres in ischemic stroke modeling in miniature pigs

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Neural Regeneration Research 2013年第16期8卷 1473-1480页

作者： Yongchun Cui Yi Tian Yue Tang Liujun Jia Aili Wu Peng Peng Jianzhong Yang Hong Du Xiaojuan Wang Like Wu State Key Laboratory of Cardiovascular Disease Fuwai Hospital National Center for Cardiovascular Diseases Chinese Academy of Medical Sciences and Peking Union Medical College Wu Stem Cells Medical Center Beijing Union Health Co. Ltd.

The miniature pig is an optimal animal model for studying nervous system disease because of its physiologic and pathologic features. However, the rete mirabile composed of arteries and veins at the skull base limits their application as a model of ischemic stroke by middle cerebral artery occlusion. The present study investigated the possibility of establishing an ischemic stroke model in the miniature pig by blocking the skull base retia with sodium alginate microspheres. Three Bama miniature pigs were used. Using the monitor of C-arm X-ray machine, sodium aiginate microspheres （100-300 pm）, a novel embolic material, were injected through the femoral artery, aortic arch, common carotid artery, ascending pharyngeal artery and the retia. Results were evaluated using carotid arteriography, MRI, behavior observation and histology. The unilateral rete mirabile was completely blocked, resulting in disturbance in blood supply to the basal ganglia, astasia of the dght hind limb and salivation. MRI and hematoxylin-eosin staining showed an evident infarction focus in the basal ganglia. These findings indicate that sodium alginate microspheres are a suitable embolic material for blocking the skull base retia in miniature pigs to establish an ischemic stroke models.

关键词： neural regeneration brain injury stroke miniature pig sodium alginate microsphere basilar bloodvessels middle cerebral artery grants-supported paper neuroregeneration

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Involvement of NLRP3-inflammasome pathway in noise-induced hearing loss

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Neural Regeneration Research 2022年第12期17卷 2750-2754页

作者： Na Sai Yuan-Yuan Yang Long Ma Da Liu Qing-Qing Jiang Wei-Wei Guo Wei-Ju Han College of Otolaryngology Head and Neck Surgery Chinese PLA General HospitalBeijingChina National Clinical Research Center for Otolaryngologic Diseases BeijingChina Key Laboratory of Hearing Science Ministry of EducationBeijingChina Beijing Key Laboratory of Hearing Impairment for Prevention and Treatment BeijingChina Department of Otolaryngology Head and Neck Surgery Peking University Shenzhen HospitalShenzhenGuangdong ProvinceChina PLA Rocket Force Characteristic Medical Center Department of Stomatology China

The inflammasome is a multiprotein oligomer in the cell cytoplasm and is part of the innate immune *** plays a crucial role in the pathological process of noise-induced hearing loss(NIHL).However,the mechanisms of NLR family pyrin domain containing 3(NLRP3)inflammasome activation in NIHL have not been clearly *** this study,miniature pigs were exposed to white noise at 120 dB(A)and auditory brainstem response measurements were used to measure their hearing *** staining,confocal laser scanning microscopy,western blot assay,and quantitative reverse transcription-polymerase chain reaction were used to analyze inflammasome-related protein distribution and ***3,interleukin-1β,interleukin-18,and cleaved-caspase-1 were highly expressed in the cochlea after 120 dB(A)white noise *** findings suggest that NLRP3-inflammasomes in the cochlea may be activated after acoustic trauma,which may be an important mechanism of noise-induced hearing loss.

关键词： acoustic injury inflammasome interleukin-1β miniature pig NLR family pyrin domain containing 3(NLRP3) noise-induced hearing loss sensorineural hearing loss

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Investigating the expression profiles of cysteine string proteins(CSPs)in cochlear tissue

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Journal of Otology 2024年第4期19卷 193-199页

作者： Shili Zhang Caiji Wang Yuehua Qiao Xi Shi Department of Otolaryngology-Head and Neck Surgery Linyi Central HospitalLinyi276401ShandongChina Clinical Hearing Center Affiliated Hospital of Xuzhou Medical UniversityXuzhou221006JiangsuChina The Institute of Audiology and Balance Science Xuzhou Medical UniversityXuzhouJiangsu221004China Department of Infectious Diseases Xuzhou Infectious Diseases HospitalAffiliated Hospital of Xuzhou Medical UniversityXuzhou221000China Key Laboratory of Tropical Biological Resources of Ministry of Education School of Pharmaceutical SciencesHainan UniversityHaikou570228China Academician Workstation of Hainan University(School of Pharmaceutical Sciences) Yazhou BaySanya 572000HainanChina

Objective:This study aims to explore the expression patterns of cysteine string protein alpha(CSPα)and cysteine string protein beta(CSPβ)in the mammalian inner ear,with an emphasis on their temporal dynamics during the developmental stages of C57BL/6 ***:We utilized immunofluorescence staining to assess the localization and distribution of CSPαand CSPβwithin the inner ears of C57BL/6 mice and miniature ***,this method facilitated the investigation of their temporal expression ***:In adult C57BL/6 mice and miniature pigs,CSPαand CSPβwere identified in the cytoplasm of inner hair cells and spiral ganglion cells,yet were absent in outer hair *** proteins were found to colocalize with Ctbp2 on the basal side of the cytoplasm in inner hair cells’basilar *** of CSPαwas observed at the nerve fiber termini at the basilar membrane’s base of inner and outer hair cells 10 days postnatally in C57BL/6 ***,expression of both CSPαand CSPβin the cytoplasm of inner hair cells emerged on the 12th day post-birth,aligning with the timeline for registering cochlear *** expression levels of both proteins increased with age,but were consistently absent in outer hair ***,expression of CSPαand CSPβwas present in the cytoplasm of inner hair cells in miniature pigs as early as one day post-birth,yet remained absent in the three rows of outer hair ***:CSPαand CSPβexhibit predominant and specific expression in inner hair cells and spiral ganglion cells.A unique expression pattern was observed for CSPα,which was also present at the nerve fiber endings of both inner and outer hair *** developmental expression trajectory of CSPαand CSPβin mouse inner hair cells is characterized by an initial absence,followed by a gradual ***,the timing of expression onset between mice and miniature pigs indicates distinct temporal dynamics,suggesting a potential role in auditory developme

关键词： Cysteine string protein CSPα CSPβ miniature pig C57BL/6 mouse

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Transcriptomic and proteomic studies of condylar ossification of the temporomandibular joint in porcine embryos

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Animal Models and Experimental Medicine 2023年第4期6卷 294-305页

作者： Lei Xiang Yongfeng Li Xuewen Wang HuaWei Liu Ping Chang Xiaodan Mu Tengyue Tianteng Min Hu Beijing Research Institute of Traumatology and Orthopaedics BeijingChina Department of Stomatology Beijing Friendship HospitalCapital Medical UniversityBeijingChina Institute for Laboratory Animal Resources National Institutes for Food and Drug ControlBeijingChina Department of Stomatology the First Medical Center of PLA General HospitalBeijingChina State Key Laboratory of West China College of Stomatology Sichuan UniversityCheng DuChina

Background:The ossification mechanism of the temporomandibular joint(TMJ)condyle remains unclear in human *** size and structure of TMJ,shape of articular disc and the characteristics of omnivorous chewing in the pig are similar to those of *** pig is an ideal animal for studying the mechanism of ossification of the TMJ condyle during the embryonic ***:In a previous study by our group,it was found that there was no condylar ossification on embryonic day(E)45,but the ossification of condyle occurred between E75 and *** this study,a total of 12 miniature pig embryos on E45 and E85 were *** embryos were used for tissue sections(3 in each group).The remaining six embryos were used for transcriptomic and proteomic studies to find differential genes and *** differentially expressed genes in transcriptome and proteomic analysis were verified by ***:In total,1592 differential genes comprising 1086 up-regulated genes and 506 down-regulated genes were screened for fold changes of≥2 to≤0.5 between E45 and *** the total of 4613 proteins detected by proteomic analysis,there were 419 differential proteins including 313 up-regulated proteins and 106 down-regulated proteins screened for fold changes of≥2 to≤0.5 between E45 and E85.A total of 36 differential genes differing in both transcriptome and proteome analysis were *** analysis showed that 14 of 15 selected genes were consistent with transcriptome ***:Condylar transcriptome and proteomic analysis during the development of TMJ in miniature pigs revealed the regulatory genes/proteins of condylar ossification.

关键词： condyle embryo miniature pig ossification proteomics transcriptome

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