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Mitochondria-targeted ratiometric fluorescent probes for micropolarity and microviscosity and their applications

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Chinese Chemical Letters 2019年第5期30卷 1071-1074页

作者： Jingjing Cui Yuhua Yao Cong Chen Rui Huang Weibing Zhang Junhong Qian Shanghai Key Laboratory of Functional Materials Chemistry School of Chemistry & Molecular Engineering East China University of Science and Technology

Two fluorescent“off-on”probes YYH1 and YYH2 were used for the detection of mitochondrial polarity and *** probes have two emission bands:The peak of shorter wavelength was sensitive to the polarity,while that in longer wavelength region was sensitive to the viscosity,which enables us to detect medium polarity and viscosity *** lg(Ishort/Ilong)of YYH1 exhibited linear relationship to the solvent polarity,while that of YYH2 was closely related to the solvent ***,both probes showed potential in bioimaging mitochondrial polarity or viscosity in living cells.

关键词： Fluorescent probe Mitochondria microviscosity Micropolarity

A likely role for the PH-domain containing protein, PEPP2/ PLEKHA5, at the membrane-microtubule cytoskeleton interface

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BIOCELL 2013年第3期37卷 55-61页

作者： Yi ZOU Timothy C COX School of Molecular Biosciences University of AdelaideAdelaideSouth Australia and Department of Anatomy&Developmental BiologyMonash UniversityClaytonVictoriaAustralia

PH(pleckstrin homology)domains are well known to bind membrane phosphoinositides with different specificities and direct PH domain-containing proteins to discrete subcellular compartments with assistances of alternative binding *** domain-containing proteins have been found to be involved in a wide range of cellular events,including signalling,cytoskeleton rearrangement and vesicular *** we showed that a novel PH domain-containing protein,PEPP2(also known as PLEKHA5),displays moderate phosphoinositide binding *** length PEPP2 was observed to variably associate with both the plasma membrane and *** membrane-associated PEPP2 nucleated at cell-cell contacts and the leading edge of migrating *** of PEPP2 increased membrane microviscosity,indicating a potential role for PEPP2 in regulating function of microtubule-dependent membrane functions.

关键词： microtubule membrane phosphoinositide microviscosity PH domain

A likely role for a novel PH-domain containing protein,PEPP2,in connecting membrane and cytoskeleton

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BIOCELL 2012年第3期36卷 127-132页

作者： YI ZOU WENPING ZHONG Department of Biology School of Life Science and TechnologyJinan UniversityGuangzhouChina

PH domains(pleckstrin homology)are well known to bind membrane phosphoinositides with different specificities and direct PH domain-containing proteins to discrete subcellular apartments with assistances of alternative binding *** domain-containing proteins are found to be involved in a wide range of cellular events,including signalling,cytoskeleton rearrangement and vesicular *** we showed that a novel PH domain-containing protein,PEPP2,displayed moderate phosphoinositide binding *** length PEPP2 associated with both plasma membrane and *** membrane-associated PEPP2 nucleated at cell-cell contacts and the leading edge of migrating *** of PEPP2 increased membrane microviscosity,indicating a potential role of PEPP2 in regulating function of membrane and microtubules.

关键词： microtubule membrane phosphoinositide microviscosity wortmannin

Features of Cellular,Humoral and Activation Markers of Immune System and Biophysical Parameters of Superficial Membranes of Lymphocytes of Periphery Blood at Patients with a Breast Cancer

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海外英语 2010年第4X期 202-207页

作者： Alimhodzhaeva L. T. The Republican Research Center of Oncology under the Ministry of Health of the Republic of Uzbekistan Tashkent

Studied features of cellular and humoral factors of immune system and activation markers of lymphocytes at breast cancer *** biophysical parameters of lymphocyte superficial membranes of periphery blood of breast cancer *** of immune system reaction were:expressed Т - cellular immunodeficiency,deficiency of CD4+Т-helpers/inducers against raised expression of СD8+Т-lymphocyte,suppression of immunoregulatory index,imbalance of immunity humoral link,activity of expression CD16 + and CD56 + on natural killers and rising of expression CD38 + and CD95 + on *** of biophysical parameters of lymphocytes superficial membranes has allowed to reveal statistically significant changes characterized by intensifying of electric field and rising ofmicroviscosity,obviously leading to disappearance or weakening of intercellular interactions.

关键词： cellular immunity humoral immunity innovation markers of lymphocytes microviscosity of membranes a superficial charge of membranes of lymphocytes breast cancer.

Lipid-Protein Microinclusions in the Morphological Structures of Organelle Membranes Studied by Fluorescent Confocal Microscopy

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Advances in Biological Chemistry 2017年第1期7卷 42-59页

作者： Michael Yu. Chernyshov Vadim N. Nurminsky Natalia V. Ozolina Presidium of Irkutsk Scientific Center Siberian Branch Russian Academy of Sciences Irkutsk Russia Siberian Institute of Plant Physiology and Biochemistry Siberian Branch Russian Academy of Sciences Irkutsk Russia

Peculiar properties of morphological structures of organelle membranes were studied by fluorescent confocal microscopy. The list of objects in our experiments was represented by mitochondria, chloroplasts and vacuoles. During this study, identification of lipid microinclusions having the form of such lipid-protein structural microformations as lipid-protein microdomains, vesicles and membrane tubular structures (cytoplasmic transvacuolar strands and nanotubes) located in organelle membranes or bound up with them was conducted. Such membrane probes as laurdan, DPH, ANS and bis-ANS were used. Comparison of fluorescence intensity of these membrane probes was conducted. This investigation of the morphological properties of lipid-protein structural microformations was accompanied with analysis of 1) the phase state and 2) dynamics of microviscosity variations in the membrane elements of isolated plant cell organelles. Distributions of laurdan fluorescence generalized polarization (GP) values for the membrane on the whole and for the intensively fluorescing membrane segments were obtained. It was discovered that the microviscosity of intensively fluorescing membrane segments essentially differed from the microviscosity of the rest part of the membrane. In conclusion, some results of the study of peculiar properties of lipid-protein structural microformations related to the structure of organelle membranes and the discoveries made in this investigation are discussed.

关键词： Identification of Lipids Morphological Structure of Cell Organelles Morphological Structure of Vacuolar Membrane Fluorescent Confocal Microscopy Fluorescent Probes Lipid-Protein Structural Membrane Microinclusions Types of Microformations Identified Lipid-Protein Microdomains Lipid-Protein Membrane Nanotubes Vesicles Cytoplasmic Strands microviscosity

An Investigation on the Polymerization of Styrene by Fluorescence Probe Technique 

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Journal of Southeast University(English Edition) 1999年第2期15卷 84-87页

作者：李新松王国昌何炳林东南大学化学化工系南京210096 南开大学高分子研究所天津300071

The polymerization of styrene is monitored by pyrene excimer formation. The ratio of monomer to excimer intensities ( I m/ I e) of pyrene increases as polymerization proceeds. The increase of I m/ I e is ascribed to the increase of microviscosity surrounding the probes forming excimer during polymerization. The linear relationship between the changing rate of I m/ I e and the polymerization rate of styrene is obtained. Therefore, I m/ I e may be used to monitor the progress of the polymerization of styrene.

关键词： excimer fluorescence polymerization microviscosity styrene