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Characteristics of N^(6)-methyladenosine Modification During Sexual Reproduction of Chlamydomonas reinhardtii

Genomics, Proteomics & Bioinformatics 2023年第4期21卷 756-768页

作者： Ying Lv Fei Han Mengxia Liu Ting Zhang Guanshen Cui Jiaojiao Wang Ying Yang Yun-Gui Yang Wenqiang Yang Photosynthesis Research Center Key Laboratory of PhotobiologyInstitute of BotanyChinese Academy of SciencesBeijing 100093China China National Botanical Garden Beijing 100093China College of Advanced Agricultural Sciences University of Chinese Academy of SciencesBeijing 100049China CAS Key Laboratory of Genomic and Precision Medicine Beijing Institute of GenomicsChinese Academy of Sciences and China National Center for BioinformationBeijing 100101China Sino-Danish College University of Chinese Academy of SciencesBeijing 100049China Institute of Stem Cell and Regeneration Chinese Academy of SciencesBeijing 100101China Innovative Academy of Seed Design Chinese Academy of SciencesBeijing 100093China

The unicellular green alga Chlamydomonas reinhardtii(hereafter Chlamydomonas)possesses both plant and animal attributes,and it is an ideal model organism for studying fundamental processes such as photosynthesis,sexual reproduction,and life cycle.N^(6)-methyladenosine(m^(6)A)is the most prevalent mRNA modification,and it plays important roles during sexual reproduction in animals and plants.However,the pattern and function of m^(6)A modification during the sexual reproduction of Chlamydomonas remain unknown.Here,we performed transcriptome and methylated RNA immunoprecipitation sequencing(MeRIP-seq)analyses on six samples from different stages during sexual reproduction of the Chlamydomonas life cycle.The results show that m^(6)A modification frequently occurs at the main motif of DRAC(D=G/A/U,R=A/G)in Chlamydomonas mRNAs.Moreover,m^(6)A peaks in Chlamydomonas mRNAs are mainly enriched in the 30 untranslated regions(30 UTRs)and negatively correlated with the abundance of transcripts at each stage.In particular,there is a significant negative correlation between the expression levels and the m^(6)A levels of genes involved in the microtubule-associated pathway,indicating that m^(6)A modification influences the sexual reproduction and the life cycle of Chlamydomonas by regulating microtubule-based movement.In summary,our findings are the first to demonstrate the distribution and the functions of m^(6)A modification in Chlamydomonas mRNAs and provide new evolutionary insights into m^(6)A modification in the process of sexual reproduction in other plant organisms.

关键词： N6-methyladenosine m^(6)A sequencing Sexual reproduction microtubule-associated pathway Photosynthesis Chlamydomonas reinhardtii

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