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An examination of handwritten signatures forged using photosensitive signature stamp

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Forensic Sciences Research 2021年第2期6卷 168-182页

作者： Zhen Li Xinlai Liu Department of Documents examination criminal investigation Police University of chinashenyangChina

Signature examination is the most common examination performed by any document *** of the authenticity of a handwritten signature on a questioned document is an important task for forensic document examiners in the forensic science *** a result of continuous developments in technology,a signature stamp can now be created using a photosensitive seal to enable the reproduction of a handwritten *** stamps are commonly used in China and several other *** this study,10 types of black photosensitive stamp-pad ink,10 brands of fountain pen ink,15 types of black gel ink and six types of black erasable gel ink found on the Chinese domestic market were collected and 10 photosensitive signature stamps were created using the signatures of 10 *** analysis,infrared(IR)and fluorescence analyses and microspectrophotometry(MSP)techniques were used to examine the resulting photosensitive signature stamp impressions when applied to printing papers,writing papers and invoice *** comparing the printing and spectral characteristics of the photosensitive signature stamp impressions with those of the signatures executed using the fountain pens,gel pens and erasable gel pens,it was possible to determine whether each signature was written or stamped using a photosensitive signature *** validate these results,a 96.7%absolute accuracy and a 99.3%detection rate were achieved over a total of 150 blind tests conducted by five forensic document examiners,thus demonstrating that a combination of the four analysis methods used in this work can provide a more scientific approach and improve the accuracy and the detection rate of the examination process.

关键词： Forensic sciences questioned document forensic document examination signature examination photosensitive stamp microspectrophotometry IR analysis fluorescence analysis

Intracellular levels of calmodulin are increased in transformed cells

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Cell Research 1992年第2期2卷 119-127页

作者： WANG RUIHONG, HONGQING ZHANG, SUWEN LI AND SHAQBAI XUEDepartment of Biology, Beijing Normal University, Beijing 100875, China Department of Biology Beijing Normal University Beijing China

By using Hoechst 33342,rabbit anti calmodulin anti-body,FITC-labeled goat anti rabbit IgG and SR101(sulforhodamine 101)simultaneously to stain individual normaland transformed cells,the microspectrophotometric analysisdemonstrated that 3 markers which represented the nucleus,calmodulin and total protein respectively,could be recog-nized in individualj cells without *** phase ofthe cell cycle was determined by DNA content(Hoechst33342).We found that in transformed cells(NIH3T3)tsRSV-LA90,cultured at 33℃ and transformed C3H10T1/2 cells),the ratio of calmodulin to total protein(based onthe phases of cell cycle)was higher than that in normal cells(NIH3T3 tsRSV-LA90 cells,cultured at 39℃ andC3H10T1/2 cells)in every cell cycle *** ratio in-creased obviously only from Gto S phase in either normalor transformed *** results showed that calmodulinreally increased during the transformation,and its increasewas *** the meantime when cells proceeded from Gto S,the intracellular calmodulin content also increasedspecifically.

关键词： calmodulin microspectrophotometry transformation cell cycle.

DNA Cytophotometric Measurements on Canine Stomach Carcinogenesis Induced by N-Ethyl-N'-Nitro-N-Nitrosoguanidine

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医学研究生学报 1989年第4期2卷 1-7页

作者：朱人敏王年吉萧树东江绍基 Department of Gastroenterology Jinling Hospital Nanjing China Shanghai Institute of Digestive Disease Shanghai China

Measurement of DNA content and nuclear area by microspectrophotometry was performed in gastric carcinogenesis of three adult wolfdogs induced by N-Ethyl-N’-Nitro-N-Nitrosoguanidine (ENNG) . The mean values and standard deviations of nuclear DNA and the lesions in the area of different groups were as follows: normal gastric mucosa 10.03±2.30 (arbitrary unit, AU) and 28.76±5.85 (urn2) , atrophic gastritis 12.04 ±3.34 AU and 28.69±8.02μm^2; mild dysplasia 13.52±3.73 AU and 28.23±8.12μm^2; moderate dysplasia 20.88±4.57 AU and 47.58± 10.74μm^2; severe dysplasia 24.01±4.48 AU and 56.64±12.53μm^2; well-differentiated adenocarcinoma 33.07±9.38 AU and 72.99±15.57μm^2, respectively. The nuclear DNA content and nuclear area showed a significant statistical difference between normal gastric mucosa and gastric carcinoma (P<0.01) . The nuclear area of gastric carcinoma increased with DNA content (r =0.73, P<0.01) , and the distribution patterns of DNA content in the histogram showed that diploidy was decreased and polyploidy was increased in cancer. These findings indicated that DNA ploidy patterns and nuclear area could be a useful index in differentiated carcinoma and precancerous lesions.

关键词： DNA content microspectrophotometry experimental gastric carcinoma DNA ploid pattern ENNG

DNA CYTOPHOTOMETRIC MEASUREMENTS OF CANINE STOMACH CARCINOGENESIS INDUCED BY N-ETHYL-N'-NITRO-N-NITROSO GUANIDINE

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Medical Bulletin of Shanghai Jiaotong University 1990年第1期13卷 95-100页

作者：朱人敏萧树东江绍基王瑞年刘文忠胡运彪 Shanghai Institute of Digestive Disease SSMU Shanghai

Measurement of DNA content and nuclear area by microspectrophotometry was performed in gastric carcinogenesis of three adult wolfdogs induced by N-ethyl-N’-nitro-N-nitrosoguanidine (ENNG). The mean values and standard deviations of nuclear DNA and area in different groups of lesions were as follows: normal gastric mucosal 0.03±2.30 AU and 28.76±5.85 μm^2; atrophic gastritis 12.04±3.34 AU and 28.69±8.02/μm^2; mild dysplasia 13.52±3.73 AU and 28.23±8.12μm^2; moderate dysplasia 20.88±4.57 AU and 47.58±10.74 μm^2; severe dysplasia 24.01±4.48 AU and 56.64±21.53μm^2; well-differentiated adenocarcinoma 33.07±9.38 AU and 72.99±15.57μm^2, respectively. The nuclear DNA content and nuclear area showed a significant difference statistically between normal gastric mucosa and gastric carcinoma (P<0.01). The nuclear area of gastric carcinoma increased with DNA content (r=0.73, P<0.01), and the distribution patterns of DNA content in the histogram showed that diploidy was decreased and polyploidy was increased in carcinoma. These findings indicate that DNA ploidy patterns and nuclear area could be a useful index in differentiated carcinoma and precancerous lesions.

关键词： DNA content microspectrophotometry ploidy experimental gastric carcinoma ENNG

Genetic variation of Norway spruce clones regarding their natural durability, physical and chemical properties

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Advances in Bioscience and Biotechnology 2012年第8期3卷 1104-1112页

作者： Ilze Irbe Guna Noldt Uldis Grinfelds Anrijs Verovkins Aris Jansons Gerald Koch Johann Heinrich von Thunen-Institute (vTI)/Federal Research Institute for Rural Areas Forestry and Fisheries Institute of Wood Technology and Wood Biology (HTB) Hamburg Germany Latvian State Forest Research Institute “Silava” Salaspils Latvia Latvian State Institute of Wood Chemistry (LS IWC) Riga Latvia

Genetic variation of ten Norway spruce (Picea abies (L.) Karst.) clones regarding their decay resistance against brown rot fungi, as well as physical and chemical properties of clones were investigated. 31- year-old spruce clones: 26, 31, A10, A15, A7, B10, B15, B6, V7, V9 were selected across Latvia. The stem diameters of spruce clones varied between 13.0 and 20.9 cm. The wood density of clones ranged from 361 to 443 kg/m3. Klason lignin content, depending on the clone, was between 27.0%-28.9%. Cellular UV microspectrophotometry of the non-infected tracheids displayed the typical lignin distribution with highest absorbance values in the cell corners (abs280 nm 0.80) and compound middle lamellae (abs280 nm 0.48), while secondary wall showed lower lignin absorbance values (abs280 nm0.29 - 0.35). The deposition of phenolic extractives in ray parenchyma and epithelial cells of resin canals were emphasized by a significantly higher UV-absorbance (abs280 nm 0.68 to 0.78) when compared to the cell wall associated lignin. The content of acetone-soluble extractives of spruce clones varied between 1.1% - 1.8%. The x-value (natural durability) for all spruce clones after exposure to C. puteana and P. placentawas >0.90 (durability class 5, not durable). Most of clones after degradation by G. trabeum had x-value > 0.90 with exception of clones B15 and V9 that showed x-value ≤ 0.90 (durability class 4, slightly durable). Natural durability of spruce clones did not correlate with stem diameter, density, content of lignin and extractives.

关键词： Norway Spruce Clones Natural Durability Brown Rot Lignin Extractives Density UV microspectrophotometry

Main Anatomy Characteristics in Cell Wall and Lignin Distribution of Bamboo Culms(Pseudosasa amabilis)

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Chinese Forestry Science and Technology 2012年第3期11卷 70-71页

作者： Yang Shumin Liu Xing'e Fei Benhua Jiang Zehui Yang Xi Shan Haibin International Center for Bamboo and Rattan

The anatomy characteristics of cell wall and lignin distribution in different anatomical regions for 12-month-old bamboo species Pseudosasa amabilis was *** lignin distribution was studied by means of optical microscope and confocal laser scanning microscope and the color reaction. In addition,the distribution of lignin in different tissues(fiber,parenchyma and vessel) was investigated by visible-light microspectrophotometry coupled with the Wiesner and Maule *** cell walls of different tissues were all lignified and the lignin was widely distributed in different tissues, in which there was a difference between tissues and anatomical regions in lignin *** lignin and syringyl lignin unit could be found in cell wall of fiber,parenchyma and vessel through Wiesner and Maule reaction and the absorbance peak of spectrum. There was no regular variation in lignin content with different radial location of culms and different location in fiber *** lamellation of the fiber cell wall was generally had as alternating broad and narrow layers with alternating low and high concentration of lignin.

关键词： Pseudosasa amabilis characteristics of cell wall distribution of lignin visible-light microspectrophotometry