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Life Cycle of Morels-Fusion, Mating, Hybridization?

Life Cycle of Morels-Fusion, Mating, Hybridization?

引用

多彩菌物美丽中国——中国菌物学会2019年学术年会

作者： He Xin-Sheng Zhao Miao Wang Ya-Zhou Zhang Neng Tan Ren-Hao Wang Yin Zhu Wen-Kun Chen Bo School of Life Science and Engineering Southwest University of Science and Technology

The hyphae of morels was cultured in flat plate, empty test tube and broken inclined medium. The fusion and non-fusion phenomenon between mycelia were observed in situ under the microscope. Including 9 species: Morchella sextelata, M. importuna, M. septimelata, Mel-21 and M. galilaea, Mes-16, Mes-19, Mes-20 and Mes-21. There are more than 2000 contact points between mycelia of more than 100 strains. After six years, mushroom production experiments were carried out with over 300 strains in more than 100 locations and over 500 hectares around the world. The results showed that the self-fusion of a few parts between the mycelial branches of the same monospora strain, and the fusion point did not produce new mycelia;the mycelia of the same species did not fuse, and the polyspore strain cultures of the same species did not fuse with each other;the mycelia of different species did not fuse;the incompatible mycelial contact points appeared: type II, type X, type Y, type T, typeπ, etc., the cells contacted but did not fuse;the sexual fusion between mycelia was not observed. The phenomena of death, winding, curling,knotting and swelling were observed after hyphal contact. The pure culture of morels mycelium could form asexual propagates such as conidiospores and sclerotium. The results of mushroom production test showed that the mushroom production rates of saprophytic Morchella species were 100% of more than 200 single-spore strains, 100% of more than 100 polyspores strains, less than 50% of more than 50 pure tissue isolates and less than 50% of more than 30 mixed *** were significant differences in morphology and yield of the mushroom producing strains,and none of the symbiotic Morchella strains produced mushrooms. The fruiting bodies of the same strain of mushroom-producing species showed morphological diversity after mushroom-producing. The fruiting bodies of different species after mixed sowing were the morphological characteristics of the original species. CO

关键词： Morchella hyphae Single-spore produce fruiting bodies Sexual fusion of hyphae microscopic in situ direct observation Method of mycelium culture in empty test tube

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