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Genetic variation of Chinese and Japanese wild Pacific abalone(Haliotis discus hannai)measured by microsatellite dna markers

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Acta Oceanologica Sinica 2006年第4期25卷 146-155页

作者： LI Qi KIJIMA Akihiro Key Laboratory of Mariculture of the Ministry of Education Ocean University of China Qingdao 266003 China Graduate School of Agricultural Science Tohoku University Sendai 981 - 8555 Japan

Population differentiation and relationships among three wild populations of the Pacific abalone Haliotis discus hannai collected from coastal seas around China and Japan were estimated using microsatellite dna analysis. The results obtained with six microsatellite loci showed a high genetic diversity for China and Japan populations. The mean number of alleles per locus ranged from 11.7 to 23.0, and the average of observed and expected heterozygosity ranged from 0. 656 to 0. 721, and from 0. 721 to 0. 793, respectively. The observed genotype frequencies at each locus were mostly in agreement with Hardy - Weinberg expectations with five exceptions. Significant differences were detected between Chinese and Japanese H. discus hannai populations [ Weir and Cocker-ham＇ s fixation index（ Fst ） range ： 0. 020～ 0.023 ; Slatkin＇ s fixation index （Rst） range： 0. 016～ 0. 044 ], and no obvious difference was detected between the samples of Japanese H. discus hannm populations （ Fst = 0. 002 ; Rst = 0. 007）. The level of differentiation among populations is further evidenced by the nNeighbor-joining tree topology on which the Japanese samples were closely clustered, and the Chinese population formed a separate cluster. These results suggest that care should be taken in future management of different populations.

关键词： Haliotis discus hannai genetic variation microsatellite dna

Molecular phylogenetics and population demographic history of Amphioctopus fangsiao,inferred from mitochondrial and microsatellite dna markers

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Acta Oceanologica Sinica 2023年第6期42卷 39-48页

作者： Jian Zheng Yan Tang Ran Xu Xiaoying Zhang Xiaodong Zheng Institute of Evolution&Marine Biodiversity Ocean University of ChinaQingdao 266003China Key Laboratory of Mariculture of Ministry of Education Ocean University of ChinaQingdao 266003China

Amphioctopus fangsiao(Cephalopoda:Octopodidae)is an important commercial species in the coastal waters of *** recent years,however,the resource of *** have declined because of habitat destruction and *** analyze the genetic variations of *** caused by the fluctuation of resources,the population genetic structure of nine sampling locations collected from the Bohai Sea to the South China Sea were investigated,using mtdna COI fragments and microsatellite *** results of F-statistics,AMOVA,STRUCTURE and PCA analyses showed three phylogeographic clades(Clades A,B and C),revealing limited genetic exchange between north and south *** clades diverged in 2.23(Clades A and B)and 3.67(Clades A,B and C)million years ago,during the dramatic environmental fluctuations,such as sea level and temperature changes,have exerted great influence on the survival distribution pattern of global *** results for low genetic connectivity among *** populations provide insights into the development of management strategies,that is,to manage this species as separate management unit.

关键词： genetic diversity population genetic structure Amphioctopus fangsiao mitochondrial dna microsatellite dna

Isolation and Characterization of 15 microsatellite dna Loci for the Alpine Stream Frog Scutiger boulengeri (Anura： Megophryidae)

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Asian Herpetological Research 2016年第4期7卷 298-300页

作者： Zhiheng DU Chunzhu XU Fangyong NING Northeast Agricultural University Harbin 150030 Heilongjiang China

Fifteen microsatellite dna markers were developed from a transcriptome assembly of the alpine stream frog （Scutiger boulengeri）.To characterize these loci, we genotyped 23 individuals collected from two sites. Eleven loci were variable, with the number of alleles ranging from one to six within each population. The expected and observed heterozygosities ranged from 0 to 0.78 and from 0 to 0.64, respectively. None of the loci was in linkage disequilibrium and one locus for one population was deviated from will offer useful tools in detecting population structure Tibetan Plateau, where the species resides. the Hardy-Weinberg equilibrium. We hope that these markers of S. boulengeri and in monitoring the fragile ecosystem of the

关键词： Alpine stream frogs microsatellite dna Tibetan Plateau Scutiger boulengeri

Prediction of the Best Three-Breed Hybridized Combination of Imported Meat Sheep Using Genetic Polymorphism of microsatellite dna

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Agricultural Sciences in China 2010年第8期9卷 1194-1200页

作者： ZHANG Ying-jie LIU Yue-qin LIU Jie LI Qin-qin SUN Hong-xin College of Animal Science and Technology Agricultural University of Hebei Baoding 071000 P.R.China College of Animal Science and Technology Northwest Agricultural & Forestry University Yangling 712100 P.R.China Hebei Provincial Animal Husbandry and Veterinary Institute Baoding 071000 P.R. China

The allelic frequency, the polymorphic information contents （PIC）, the number of effective alleles, the heterozygosity, and the genetic distances were studied in three imported meat sheep （Suffolk, Dorset, Texel） and their F1 crossbred obtained from those crossed with indigenous Small Tail Hun Sheep （Suffolk♂× Small Tail Hun Sheep, SH; Dorset ♂× Small Tail Han Sheep♂, DH; Texel♂× Small Tail Hart Sheep ♀, TH） using six microsatellite dna loci. The perpormences of three-breed crossbred （Suffolk ♂× DH ♀, Suffolk ♂× TH ♀, Texel ♂× SH ♀, Texel ♂× DH ♀, Dorset ♂× TH ♀, and Dorset ♂× SH ♀ ） were tested. The results indicated that there were genetic polymorphisms at six microsatellite loci in six sheep populations. Six microsatellite loci could be used for genetic diversity evaluation in sheep populations. The order of three-breed heterosis by the analysis of genetic relationship from large to small was Texel ♂× DH ♀, Suffolk ♂× DH ♀, Suffolki ♂× TH ♀, Texel ♂× SH ♀, Dorset ♂×TH ♀, and Dorset ♂× SH ♀, which was in accordance with the testing results on actual heterosis. These results showed that prediction of the best three-breed hybridized combination among sheep breeds by microsatellite dna polymorphism was feasible, which will have an important value on the reasonable utilization of introduced meat sheep and sheep breeding in our country in the future.

关键词： sheep microsatellite dna heterosis three-breed hybridized combination

Morphological and microsatellite dna diversity of Nigerian indigenous sheep

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Journal of Animal Science and Biotechnology 2013年第1期4卷 18-33页

作者： Brilliant O Agaviezor Sunday O Peters Mufliat A Adefenwa Abdulmojeed Yakubu Olufunmilayo A Adebambo Michael O Ozoje Christian ON Ikeobi Matthew Wheto Oyeyemi O Ajayi Samuel A Amusan Oludotun J Ekundayo Timothy M Sanni Moses Okpeku Gbolabo O Onasanya Marcos De Donato Babatunde M Ilori Kadir Kizilkaya Ikhide G Imumorin Department of Animal Breeding and Genetics University of Agriculture Department of Animal Science and Fisheries University of Port Harcourt Dept of Animal Science Cornell University Dept of Animal Science Berry College Department of Cell Biology and Genetics University of Lagos Department of Animal Science Nasarawa State University Dept of Livestock Production Niger Delta University Department of Animal Science Adnan Menderes University

Background： Sheep is important in the socio-economic lives of people around the world. It is estimated that more than half of our once common livestock breeds are now endangered. Since genetic characterization of Nigerian sheep is still lacking, we analyzed ten morphological traits on 402 animals and 15 microsatellite dna markers in 384 animals of the 4 Nigerian sheep breeds to better understand genetic diversity for breeding management and germplasm conservation. Results： Morphological traits of Uda and Balami were significantly （P 〈 0.05） higher than Yankasa, which were both higher than West African Dwarf （WAD） sheep. Stepwise discriminant analysis showed tail length, rump height, chest girth, ear length and chest depth as the most discriminating variables for classification. Mahalanobis distances show the least differentiation between Uda and Balami and the largest between WAD and Balami sheep. While 93.3% of WAD sheep were correctly assigned to their source genetic group, 63.9% of Yankasa, 61.2% of Balami and 45.2% of Uda were classified correctly by nearest neighbour discriminant analysis. The overall high Polymorphism Information Content （PIC） of all microsatellite markers ranged from 0.751 to 0.927 supporting their use in genetic characterization. Expected heterozygosity was high for all loci （0.783 to 0.93）. Mean heterozygote deficiency across all populations （0.17] to 0.534） possibly indicate significant inbreeding （P 〈 0.05）. Mean values for FST, FIT and F^s statistics across all loci were 0.088, 0.394 and 0.336 respectively. Yankasa and Balami are the most closely related breeds （DA = 0.184） while WAD and Balami are the farthest apart breeds （DA-- 0.665）, which is coincident with distance based on morphological analysis and population structure assessed by STRUCTURE. Conclusions： These results suggest that within-breed genetic variation in Nigerian sheep is higher than between-breeds and may be a valuable tool for genetic improvement and conservation. The

关键词： Discriminant analysis Genetic distance microsatellite dna Morphological traits Nigerian sheep

Forecast of the Heterosis of Imported Meat Sheep by Genetic Polymorphism of microsatellite dna

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Agricultural Sciences in China 2007年第5期6卷 634-640页

作者： ZHANG Ying-jie LIU Yue-qin SUN Hong-xin SUN Shao-hua LI Wu College of Animal Science and Technology Agricultural University of Hebei Baoding 071000 P.R.China Institute of Animal Husbandry and Veterinary of Hebei Province Baoding 071000 P.R.China

Forecast of the heterosis of Small Tail Han sheep crossed with imported meat sheep by genetic polymorphism of microsatellite dna was done in different sheep breeds. The gene frequency, the polymorphism information contents, the number of effective alleles, the heterozygosity, and the genetic distances were studied in four imported meat sheep and Small Tail Han sheep using five microsatellite loci. The crossing effects on the Small Tail Han sheep with four imported meat sheep were tested. The results indicate that there are genetic polymorphisms at five microsatellite loci in five sheep breeds. Five microsatellite loci can be used for genetic diversity evaluation in sheep breeds. The genetic variability of Dorset is the highest, and that of the Small Tail Han sheep is the lowest in the five sheep breeds. The order of heterosis from large to small in four imported meat sheep by the analysis of genetic relationship is White-Suffolk, Black-Suffolk, Dorset, and Texel. This accords with the testing results of actual heterosis. It is feasible to forecast the heterosis of Small Tail Han sheep crossed with imported meat sheep by genetic polymorphism of microsatellite dna, which will have an important value for sheep breeding in the future.

关键词： sheep microsatellite dna genetic polymorphism heterosis

microsatellite dna variation among collections of bronze gudgeon Coreius heterodon in the mainstem of the Yangtze River,China

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Microsatellite DNA variation among collections of bronze gud...

Preliminary Study on Applicability of microsatellite dna Primers from Parasite Protozoa Trypanosoma cruzi in Free-living Protozoa

中国海洋湖沼学会鱼类学分会、中国动物学会鱼类学分会2012年学术研讨会

作者： Fei Cheng Wei Li Qingjiang Wu Eric Hallerman Songguang Xie Key Laboratory of Biodiversity and Conservation of Aquatic Organisms Institute of HydrobiologyChinese Academy of Sciences Wuhan Botanical Garden Chinese Academy of Sciences Department of Fish and Wildlife Conservation Virginia Polytechnic Institute and State University

Bronze gudgeon Coreius heterodon is an endemic and economically important fish in the Yangtze River whose abundance has declined dramatically because of dam construction,overfishing,and water *** collected bronze gudg... 详细信息

关键词： Coreius heterodon bronze gudgeon microsatellite dna population differentiation Yangtze River

来源： cnki会议评论

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cnki会议

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Journal of Ocean University of China 2004年第1期3卷 80-84页

作者： ZHANGWenjing YUYuhe SHENYunfen MIAOWei FENGWeisong InstituteofHydrobiology ChineseAcademyofSciencesWuhan430072P.R.China

In this paper, we took the lead in studying on specificity of the microsatellite dna loci and applicability of mi crosatellite dna primers in protozoa. In order to study characters of microsatellites in free living protozoa, eight microsatellite loci primers developed from Trypanosoma cruzi (MCLE01, SCLE10, MCLE08, SCLE11, MCLF10, MCLG10, MCL03, MCL05) were employed to amplify microsatellite in four free living protozoa, including Bodo designis, Euglena gracilis FACHB848, Paramecium bruzise and Tetrahymena thermophila BF1. In the amplification systems of P. bruzise, four loci (SCLE10, SCLE11, MCLF10, MCL03) were amplified successfully, and four amplification fragments were in proper size. In genome of E. gracilis FACHB848, five of eight primers brought five clear amplification bands. In B. designis, three (No.4, 5 and 7) of eight loci produced clear and sharp products without stutter bands, whereas no bands appeared in T. thermophila BF1. Further, eight 300-500 bp amplification fragments were cloned and sequenced. Nevertheless, all sequenced products did not contain corresponding microsatellite sequence, although Bodo is in the same order and has the nearest phylogenetic relation with Trypanosoma among these four species. Thus, the microsatellite dna primers can not be applied among order or more far taxa, and the specificity of microsatellite dna is very high in protozoa. The results of this study will contribute to our understanding of microsatellite dna in protozoa.

关键词： protozoa microsatellite dna amplification applicability of primers

Isolation and Characterization of microsatellite dna Makers from Amphioxus Branchiostoma belcheri

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Isolation and Characterization of Microsatellite DNA Makers ...