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Lipid-Protein Microinclusions in the Morphological Structures of Organelle Membranes Studied by Fluorescent Confocal Microscopy

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Advances in Biological Chemistry 2017年第1期7卷 42-59页

作者： Michael Yu. Chernyshov Vadim N. Nurminsky Natalia V. Ozolina Presidium of Irkutsk Scientific Center Siberian Branch Russian Academy of Sciences Irkutsk Russia Siberian Institute of Plant Physiology and Biochemistry Siberian Branch Russian Academy of Sciences Irkutsk Russia

Peculiar properties of morphological structures of organelle membranes were studied by fluorescent confocal microscopy. The list of objects in our experiments was represented by mitochondria, chloroplasts and vacuoles. During this study, identification of lipid microinclusions having the form of such lipid-protein structural microformations as lipid-protein microdomains, vesicles and membrane tubular structures (cytoplasmic transvacuolar strands and nanotubes) located in organelle membranes or bound up with them was conducted. Such membrane probes as laurdan, DPH, ANS and bis-ANS were used. Comparison of fluorescence intensity of these membrane probes was conducted. This investigation of the morphological properties of lipid-protein structural microformations was accompanied with analysis of 1) the phase state and 2) dynamics of microviscosity variations in the membrane elements of isolated plant cell organelles. Distributions of laurdan fluorescence generalized polarization (GP) values for the membrane on the whole and for the intensively fluorescing membrane segments were obtained. It was discovered that the microviscosity of intensively fluorescing membrane segments essentially differed from the microviscosity of the rest part of the membrane. In conclusion, some results of the study of peculiar properties of lipid-protein structural microformations related to the structure of organelle membranes and the discoveries made in this investigation are discussed.

关键词： Identification of Lipids Morphological Structure of Cell Organelles Morphological Structure of Vacuolar Membrane Fluorescent Confocal Microscopy Fluorescent Probes Lipid-Protein Structural Membrane Microinclusions Types of microformations Identified Lipid-Protein Microdomains Lipid-Protein Membrane Nanotubes Vesicles Cytoplasmic Strands Microviscosity

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