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Evaluation of curcumin and copper acetate against Salmonella Typhimurium infection,intestinal permeability,and cecal microbiota composition in broiler chickens

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Journal of Animal Science and Biotechnology 2021年第3期12卷 1019-1030页

作者： Anaisa ALeyva-Diaz Daniel Hernandez-Patlan Bruno Solis-Cruz Bishnu Adhikari Young Min Kwon Juan D.Latorre Xochitl Hernandez-Velasco Benjamin Fuente-Martinez Billy M.Hargis Raquel Lopez-Arellano Guillermo Tellez-Isaias Departamento de Medicina y Zootecnia de Aves Facultad de Medicina Veterinaria y ZootecniaUNAM04510 Ciudad de MexicoMexico Laboratorio 5:LEDEFAR Unidad de Investigacion MultidisciplinariaFacultad de Estudios Superiores(FES)CuautitlanUniversidad Nacional Autonoma de Mexico(UNAM)54714 Cuautitlan IzcalliMexico Department of Poultry Science Center of Excellence for Poultry ScienceUniversity of Arkansas1260 W.MaplePOSC 0-114FayettevilleAR 72704USA Centro de Ensenanza Investigacion y Extension en Produccion AvicolaFacultad de Medicina Veterinaria y ZootecniaUNAMCiudad de MexicoMexico

Background:Interest in the use of natural feed additives as an alternative to antimicrobials in the poultry industry has increased in recent years because of the risk of bacterial resistance.One of the most studied groups are polyphenolic compounds,given their advantages over other types of additives and their easy potentiation of effects when complexes are formed with metal ions.Therefore,the objective of the present study was to evaluate the impact of dietary supplementation of copper acetate(CA),curcumin(CR),and their combination(CA-CR)against Salmonella Typhimurium colonization,intestinal permeability,and cecal microbiota composition in broiler chickens through a laboratory Salmonella infection model.S.Typhimurium recovery was determined on day 10 post-challenge by isolating Salmonella in homogenates of the right cecal tonsil(12 chickens per group)on Xylose Lysine Tergitol-4(XLT-4)with novobiocin and nalidixic acid.Intestinal integrity was indirectly determined by the fluorometric measurement of fluorescein isothiocyanate dextran(FITC-d)in serum samples from blood obtained on d 10 post-S.Typhimurium challenge.Finally,microbiota analysis was performed using the content of the left caecal tonsil of 5 chickens per group by sequencing V4 region of 16S rRNA gene.Results:The results showed that in two independent studies,all experimental treatments were able to significantly reduce the S.Typhimurium colonization in cecal tonsils(CT,P<0.0001)compared to the positive control(PC)group.However,only CA-CR was the most effective treatment in reducing S.Typhimurium counts in both independent studies.Furthermore,the serum fluorescein isothiocyanate dextran(FITC-d)concentration in chickens treated with CR was significantly lower when compared to PC(P=0.0084),which is related to a decrease in intestinal permeability and therefore intestinal integrity.The effect of dietary treatments in reducing Salmonella was further supported by the analysis of 16S rRNA gene sequences using Linear discriminant analysis effect size(LEfSe)sin

关键词： Broiler chickens Copper acetate Curcumin Intestinal permeability microbiota composition Salmonella Typhimurium

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Diverse microbiota in palatal radicular groove analyzed by Illumina sequencing:Four case reports

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World Journal of Clinical Cases 2021年第23期9卷 6846-6857页

作者： Xue-Lian Tan Xuan Chen Yu-Jie Fu Ling Ye Lan Zhang Ding-Ming Huang State Key Laboratory of Oral Diseases&National Clinical Research Center for Oral Diseases&Department of Operative Dentistry and Endodontics West China Hospital of Stomatology Sichuan UniversityChengdu 610041Sichuan ProvinceChina

BACKGROUND A palatal radicular groove is an unusual developmental deformity of the tooth,which may serve as a channel linking the periodontal and periapical inflammation,and yet no literature could be obtained analyzing microbiota within the palatal radicular grooves.CASE SUMMARY Four patients diagnosed with palatal radicular groove and concomitant periodontal-endodontic deformity in permanent maxillary lateral incisors were enrolled in this work.Twelve bacterial samples from 4 patients were collected from different parts of the palatal radicular groove during intentional replantation surgery.Illumina sequencing was performed to analyze the taxonomical composition and microbiome structure inside the palatal grooves,and 1162 operational taxonomic units were obtained.The phyla of Firmicutes and Proteobacteria predominated in most of the samples.An unknown genus from the Bacillaceae family,Lactococcus,and Porphyromonas were the most abundant genera identified.There was no difference in the microbiota richness and diversity in three sections of the groove.CONCLUSION The unique ecological niches inside the palatal grooves harbored bacterial communities that shared some component features of both the endodontic and periodontal infections.The existence of palatal groove may play an interaction bridge between the root apex and tooth cervix and thus impair the outcome of traditional therapeutic methods such as root canal treatment and periodontal management.

关键词： Palatal radicular groove High-throughput sequencing microbiota composition Taxonomy Endodontic-periodontal infection Case report

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