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Endometrial microrna Signature during the Window of Implantation Changed in Patients with Repeated Implantation Failure

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Chinese Medical Journal 2017年第5期130卷 566-573页

作者： Cheng Shi Huan Shen Li-Juan Fan Jing Guan Xin-Bang Zheng Xi Chen Rong Liang Xiao-Wei Zhang Qing-Hua Cui Kun-Kun Sun Zhu-Ran Zhao Hong-Jing Han Reproductive Medical Center Peking University People's Hospital Beijing 100044 China Department of Urology Peking University People's Hospital Beijing 100044 China Department of Biomedical Informatics School of Basic Medical Sciences Peking University Beijing 100191 China Department of Pathology Peking University People's Hospital Beijing 100044 China Department of Paediatrics Peking University People's Hospital Beijing 100044 China

Background： At present, a diagnostic tool with high specificity for impaired endometrial receptivity, which may lead to implantation failure, remains to be developed. We aimed to assess the different endometrial microrna （miRNA） signatures for impaired endometrial receptivity by microarray analysis. Methods： A total of 12 repeated implantation failure （RIF） patients and I0 infertile patients, who conceived and delivered after one embryo transfer attempt, were recruited as RIF and control groups, respectively. Endometrial specimens from the window of implantation （WOI） were collected from these two groups. MiRNA microarray was conducted on seven and five samples from the RIF and control groups, respectively. Comparative, functional, and network analyses were performed for the microarray results. Quantitative real-time polymerase chain reaction （PCR） was performed on other samples to validate the expression of specific miRNAs. Results： Compared with those in the control group, the expression levels of 105 miRNAs in the RIF group were found to be significantly up- or down-regulated （at least 2-fold） by microarray analysis. The most relevant miRNA functional sets of these dysregulated miRNAs were miR-30 family, human embryonic stern cell regulation, epithelial-mesenchymal transition, and miRNA tumor suppressors by tool for annotations ofmicrorna analysis. Network regulatory analysis found 176 miRNA-mRNA interactions, and the top 3 core miRNAs were has-miR-4668-5p, has-miR-429, and has-miR-5088. Expression levels of the 18 selected miRNAs in new samples by real-time PCR were found to be regulated with the same trend, as the result ofmicroarray analysis. Conclusions： There is a significant different expression of certain miRNAs in the WOI endometrium for RIF patients. These miRNAs may contribute to impaired endometrial receptivity.

关键词： Embryo Implantation Endometrial Receptivity microrna microarray Repeated Implantation Failure Window of Implantation

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microrna Profiling of Transgenic Mice with Myocardial Overexpression of Nucleolin

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Chinese Medical Journal 2018年第3期131卷 339-346页

作者： Qing-Lan Lyu Bi-Mei Jiang Bin Zhou Li Sun Zhong-Yi Tong Yuan-Bin Li Yu-Ting Tang Hui Sun Mei-Dong Liu Xian-Zhong Xiao Department of Pathophysiology Xiangya School of Basic Medical Sciences Central South University Changsha Hunan 410078 China Department of Rheumatology Xiangya Hospital Central South University Changsha Hunan 410008 China Department of Pathology The Second Xiangya Hospital Central South University Changsha Hunan 410000 China

Background：Nucleolin （NCL） is the most abundant RNA-binding protein in the cell nucleolus and plays an important role in chromatin stability,ribosome assembly,ribosomal RNA maturation,ribosomal DNA transcription,nucleocytoplasmic transport,and regulation of RNA stability and translation *** addition to its anti-apoptotic properties,the underlying mechanisms associated with NCL-related roles in different cellular processes remain *** this study,the effect of NCL on microrna （miRNA） expression was evaluated by generating transgenic mice with myocardial overexpression of NCL and by analyzing microarrays of mature and precursor miRNAs from ***：Using microinjection ofalpha-MyHc clone 26-NCL plasmids,we generated transgenic mice with myocardial overexpression of NCL firstly,and then mature and precursor miRNAs expression profiles were analyzed in NCL transgenic mice （n =3） and wild-type （WT） mice （n =3） by miRNA *** Package for the Social Sciences version 16.0 software （SPSS,Inc.,Chicago,IL,USA） was used to perform Student＇s t-test,and statistical significance was determined at P 〈 ***：Several miRNAs were found to be differentially expressed,of which 11 were upregulated and 4 were downregulated in transgenic mice with myocardial overexpression of NCL compared to those in WT *** differentially expressed miRNAs were subsequently confirmed and quantified by real-time quantitative reverse transcription-polymerase chain *** analysis was used for the prediction of miRNA ***,in vitro experiments showed that NCL regulated miR-21 expression following hydrogen peroxide ***：Myocardial-protection mechanisms exerted by NCL might be mediated by the miRNAs identified in this study.

关键词： microrna microarray Myocardium Nucleolin Transgenic mice

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hsa-mir-183 is frequently methylated and related to poor survival in human hepatocellular carcinoma

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World Journal of Gastroenterology 2017年第9期23卷 1568-1575页

作者： Sumadi Lukman Anwar Till Krech Britta Hasemeier Elisa Schipper Nora Schweitzer Arndt Vogel Hans Kreipe Reena Buurman Britta Skawran Ulrich Lehmann Ulrich Lehmann Institute of PathologyMedizinische Hochschule Hannover Department of Surgery Faculty of Medicine Universitas Gadjah Mada Department of Gastroenterology Hepatology and EndocrinologyMedizinische Hochschule Hannover Institute of Human Genetics Medizinische Hochschule Hannover

To screen clinically relevant micrornas (miRNAs) silenced by DNA methylation in human hepatocellular carcinoma (HCC).METHODSKnockdown of DNA methyltransferases (DNMTs) using siRNAs and miRNA profiling in HCC cell lines were performed to identify DNA hypermethylation-mediated miRNA downregulation. Confirmation using individual quantitative real-time PCR (qRT-PCR) assays was then performed followed by DNA methylation quantification at the promoter of the miRNA genes. Quantification of DNA methylation and miRNA expression was then performed in primary HCC tumor samples and related with clinicopathological *** profiling after DNMT knockdown in HCC cell lines revealed upregulation of miR-23, miR-25 and miR-183. After qRT-PCR confirmation and CpG island methylation quantification of these miRNAs in cell lines, further analysis in primary HCC specimens showed that hsa-miR-183 is hypermethylated in 30% of HCC (n = 40). Expression of mature miR-183 showed an inverse correlation with DNA methylation levels. In HCC cells, DNMT knockdown and 5-aza-2’-deoxycytidine treatment reduced methylation and stimulated expression of miR-183. In HCC patients, hypermethylation at hsa-miR-183 promoter significantly correlates with poor survival (log-rank test P = 0.03). DNA methylation analysis in healthy liver, benign liver tumors (hepatocellular adenoma and focal nodular hyperplasia) and their corresponding adjacent tissues showed absence of hypermethylation supporting the notion that aberrant methylation at hsa-miR-183 is specific for the malignant transformation of *** data indicate that hypermethylation of hsa-miR-183 is a frequent event in HCC and potentially useful as a novel surrogate diagnostic and prognostic marker.

关键词： hsa-miR-183 DNA methylation DNMT knockdown microrna microarray

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The miRNA expression profile of the uveal melanoma

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Science China(Life Sciences) 2011年第4期54卷 351-358页

作者： YANG ChengHsun Beijing Tongren Eye Center Beijing Tongren Hospital Capital Medical University Ophthalmology & Visual Science Key Laboratory

The miRNA expression profile was initially established to investigate its corresponding function in human uveal melanoma. The miRNA expression profile in human uveal melanoma was analyzed by a micro chip *** hsa-miRNA expression between four uveal melanomas and four normal uveal tissues was *** on the bioinformatic approach,chip data was analyzed to select out differentially expressed candidate ***-time quantitative PCR(RT-PCR) was used to confirm the candidate hsa-miRNAs expression in all *** results of miRNA microarray chips that matched with RT-PCR were considered as the miRNA expression which was significantly different between normal tissue and uveal *** four uveal melanomas,expressions of miRNA-20a,miRNA-106a,miRNA-17,miRNA-21,and miRNA-34a were significantly up-regulated,while miRNA-145 and miRNA-204 expression were significantly *** used miRNA microarray analysis as a fast,efficient technology to study biological *** differentially expressed miRNAs may be involved in uveal melanoma pathogenesis,and may help promote the diagnosis and treatment for uveal melanoma.

关键词： uveal melanoma miRNA microrna microarray target gene tumorigenesis

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Differentially Expressed micrornas as Potential Markers for Vital Reaction of Burned Skin

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Journal of Forensic Science and Medicine 2018年第3期4卷 135-141,I0016-I0023页

作者： Hao‑Pin Lyu Ming Cheng Jin‑Cen Liu Ming‑Yuan Ye Di Xu Jie‑Tao He Xiao‑Li Xie Qi Wang Department of Forensic Pathology School of Forensic MedicineSouthern Medical UniversityForensic Science Centre of Guangdong Provincial Public Security DepartmentSchool of Public Health and Tropical MedicineSouthern Medical UniversityGuangzhouChina Department of Toxicology School of Public Health and Tropical MedicineSouthern Medical UniversityGuangzhouChina

The identification of antemortem burns and postmortem burns is essential in forensic *** this study,microrna(miRNA)microarray analysis was conducted to identify differentially expressed miRNAs in the skin of an experimental burn *** analysis revealed 24 differentially expressed miRNAs in antemortem burned mice skin,with 19 miRNAs significantly upregulated and 5 *** on the intersection predicted using three databases(Targetscan,***,and PITA),293 potential miRNA targets were *** dysregulated miRNAs and their predicted targets were further analyzed using the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes *** functional categories and signaling pathways were enriched,including the“fc epsilon ri signaling pathway,”“endometrial cancer,”and“mTOR signaling pathway.”Expression patterns of 10 differentially expressed miRNAs were verified by reverse transcription‑quantitative polymerase chain reaction in mice *** results agreed with the results of microarray *** findings suggest that differentially expressed miRNAs revealed by microarray are potential markers for forensic molecular diagnosis of antemortem burns.

关键词： Forensic pathology microrna microarray skin burns vital reactio

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Elevated miR-155 expression induced immunosuppression via CD39+ Tregs in Sepsis patients

Elevated miR-155 expression induced immunosuppression via CD...

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中华医学会第二届重症心脏全国学术大会暨第三届西湖重症医学论坛、2015年浙江省重症医学学术年会

作者： Jingquan Liu Kai Shi Liang Xu Minhua Chen Xianghong Yang Jun Hong Bangchuan Hu Renhua Sun Department of Intensive Care Unit Zhejiang Provincial People’s Hospital Department of Respiratory Medicine The Affiliated Hospital of Hangzhou Normal University(The 2nd People’s Hospital of Hangzhou)

Background:Altered microrna profile exists in many infectious diseases such as *** a remarkable immunosuppressive effect,CD39 Tregs cells play an important role in the regulation of immune balance in *** the correlation between microrna change and the ratio of CD39 Tregs in sepsis patients has yet not been *** this study,the altered micrornas expression profile in sepsis patients was ***,the correlation between micrornas and severity of disease and prognosis was ***,the correlation between microrna and the percentage of peripheral blood CD39 Tregs was also investigated and further verified by animal ***:Sixty sepsis patients and 30 healthy controls were *** difference in microrna expression was investigated by microrna microarray and further confirmed by *** correlation between micrornas changes and SOFA score,severity of sepsis and survival was *** percentage of CD39+Tregs in peripheral blood of sepsis patients was measured by flow *** correlation between micrornas and percentage of CD39+Tregs was analyzed and further confirmed by mouse sepsis ***:Compared with the healthy controls,the sepsis patients exhibited a significant elevated microrna-155(miR-155)level(p2.47 had a lower 28 d survival(p<0.05).The miR-155 level of patients was proportional to the percentage of CD39+Tregs(r=0.637,p<0.05).After transfected with miR-155 inhibitor,the ratio of CD39+Tregs in mice with sepsis was significantly reduced(p<0.05).Conclusions:A higher level of miR-155 indicated a more severe condition and poor prognosis in sepsis *** possible underlying mechanism could be that miR-155 induced the percentage of CD39+Tregs and thus immunos

关键词： Sepsis microrna Regulatory T cell microrna microarray outcome

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