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Upregulation of miR-34c after silencing E2F transcription factor 1 inhibits paclitaxel combined with cisplatin resistance in gastric cancer cells

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World Journal of Gastroenterology 2020年第5期26卷 499-513页

作者： Hong Zheng Jin-Jing Wang Xiao-Rong Yang Yong-Lin Yu Department of Pathology the Affiliated Hospital of Zunyi Medical University

BAcKGROUND microrna 34c(miR-34c)has been reported to be associated with malignant types of cancer,however,it remains unknown whether miR-34c is involved in chemoresistance in gastric cancer(Gc).AIM To investigate the effect of miR-34c and its upstream transcription factor E2F1 on paclitaxel combined with cisplatin resistance in Gc *** Paired Gc tissues and adjacent normal tissues were randomly sampled from 74 Gc ***-34c and E2F1 were detected by real-time quantitative PcR(qPcR)and Western *** addition,the drug resistance of Gc cells to paclitaxel and cisplatin was induced by concentration gradient increasing methods,and changes in miR-34c and E2F1 during this process were ***,E2F1 and miR-34c overexpression or underexpression vectors were constructed and transfected into drug-resistant Gc *** was employed to test the sensitivity of cells to paclitaxel combined with cisplatin,qPcR was adopted to detect the expression of miR-34c,Western blot was applied to detect the expression levels of E2F1,drug resistance-related proteins and apoptosis-related proteins,and flow cytometry was used for the determination of cell apoptosis and cell cycle *** E2F1 was overexpressed while miR-34c was underexpressed in *** inducing Gc cells to be resistant to paclitaxel and cisplatin,E2F1 expression increased while miR-34c expression *** silencing E2F1 and overexpressing miR-34c could increase the sensitivity of drug-resistant Gc cells to paclitaxel combined with cisplatin,promote cell apoptosis and inhibit cell *** which,silencing E2F1 could reduce the expression of drug resistance-related proteins and apoptosis-related proteins,while over-expression of miR-34c could upregulate the expression of apoptosis-related proteins without affecting the expression of MDR-1,MRP and other drug resistance-related *** experiments demonstrated that inhibiting miR-34c could significantly weaken the sensi

关键词： E2F transcription factor 1 microrna 34c Gastric cancer Paclitaxel combined with cisplatin resistance

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