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Horticultural Plant Journal 2016年第4期2卷 209-216页

作者： WEI Xiaochun XU Wen YUAN Yuxiang YAO Qiuju ZHAO Yanyan WANG Zhiyong JIANG Wusheng ZHANG Xiaowei Institute of Horticulture Henan Academy of Agricultural Sciences Crop Designing Center Henan Academy of Agricultural Sciences

Increasing evidence has revealed that micro rnas play a pivotal role in the post transcriptional regulation of gene expression in response to pathogens in plants. However, there is little information available about the expression patterns of mi rnas and their targets in Chinese cabbage(Brassica rapa ssp. pekinensis) under Plasmodiophora brassicae stress. In the present study, using deep sequencing and degradome analysis, a genome-wide identification of mi rnas and their targets during P. brassicae stress was performed. A total of 221 known and 93 potentially novel mi rnas were successfully identified from two root libraries of one control(635-10CK) and P. brassicae-treated Chinese cabbage samples(635-10T). Of these, 14 known and 10 potentially novel mi rnas were found to be differentially expressed after P. brassicae treatment. Degradome analysis revealed that the 223 target genes of the 75 mi rnas could be potentially cleaved. KEGG(Kyoto Encyclopedia of Genes and Genomes)pathway analysis suggested that the putative target genes of the mi rnas were predominately involved in selenocompound metabolism and plant hormone signal transduction. Then the expression of 12 mi rnas was validated by quantitative real-time PCR(q RT-PCR). These results provide insights into the mi RNA-mediated regulatory networks underlying the stress response to the plant pathogen P. brassicae.

关键词： micro rnas Brassica rapa ssp pekinensis Plasmodiophora brassicae deep sequencing

The key role of micro rnas in mitochondria fusion protein 1 mediated pulmonary vascular remodeling

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The key role of micro RNAs in mitochondria fusion protein 1 ...

micro rnas与急性心肌梗死关系的研究进展

中国生理学会第24届全国会员代表大会暨生理学学术大会

作者： Cui Ma Chen Zhang Hongmin Yao Yumei Liu Lixin Zhang Mingfei Ma Daling Zhu 哈尔滨医科大学大庆校区 Department of Biopharmaceutical Sciences College of PharmacyHarbin Medical University-Daqing

AIM:The imbalance of pulmonary arterial smooth muscle cells（PASMCs）mitochondrial homeostasis induced abnormal proliferation is an important pathological process in the hypoxic pulmonary arterial hypertension,the molecular mechanism is *** hypothesis that hypoxia affect the form and functions of mitochondria,regulate the cell cycle progression,increase the cell proliferation and pulmonary vascular ***:We prove this hypothesis by the following research:1).we performed animal models,cell biology and molecular biology to determine the mitochondrial function in pathogenesis of pulmonary arterial hypertension and clarify the role of mitochondria fusion protein（Mfn1）in hypoxia induced PASMCs proliferation.2)By bioinformatics we explore the role of mi rnas in hypoxia regulated Mfn1 expression and determining the key mi RNA.3)By overexpression or knock down mi rnas,to determine the regulation of mi rnas in PASMCs mitochondrial fusion process.4)To test the effects of mi RNA on the different phases of cell cycle progression,PASMCs were performed the analyses of the cell cycle profiles,the cyclins expression after overexpression or knock down mi rnas were also ***:We found that hypoxia changed morphological of mitochondrial into vacuoles and swelling,and increased the mitochondrial fission ***1 was increased in hypoxia and monocrotaline induced pulmonary vascular *** also found that hypoxia induced smooth muscle cells proliferation in a Mfn1 dependent ***,bioinformatics results showed that Mfn1 was mediated by mi R-125 and mi ***,we studied the function of these mi rnas in PASMC apoptosis under hypoxic *** promotive effect of Mfn1 was weakened in the presence of mi R-125 and mi R-351 ***:In the present study we determined the role of mi rnas in regulating mitochondria fusion protein expression in hypoxia by bioinformatics for the the first *** results provide a the

关键词： hypoxia pulmonary vascular remodeling pulmonary arterial smooth muscle cells mitochondria fusion protein 1 micro rnas

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承德医学院学报 2024年第1期41卷 66-70页

作者：薛婷匀闫贞蓉李广妹赵佳叶孙启玉承德医学院附属医院南院区检验科河北承德067000

急性心肌梗死(acute myocardial infarction,AMI)是冠状动脉疾病最严重的表现,其引起的心肌组织损伤可促进心力衰竭的发展。尽管近些年由于生活方式的改变、治疗方式(如经皮冠状动脉介入治疗)的发展使AMI的预后得到了改善,但是AMI依旧... 详细信息

急性心肌梗死(acute myocardial infarction,AMI)是冠状动脉疾病最严重的表现,其引起的心肌组织损伤可促进心力衰竭的发展。尽管近些年由于生活方式的改变、治疗方式(如经皮冠状动脉介入治疗)的发展使AMI的预后得到了改善,但是AMI依旧每年危害着全球700多万人的身心健康,AMI仍然是世界范围内高发病率和高死亡率的主要疾病之一[1]。微小RNA(micro rnas,mirnas)是在20世纪90年代被发现的,mi rnas的研究已经迅速发展成为一个成熟而广阔的领域。mi rnas存在于几乎所有类型的细胞和细胞的病理生理活动中,包括与心血管系统相关的细胞。本文将mi rnas对AMI病理生理进程的影响进行综述,希望为临床治疗提供新思路。

关键词： micro rnas 急性心肌梗死作用机制预后

Gene regulatory network stabilized by pervasive weak repressions: micro RNA functions revealed by the May–Wigner theory

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National Science Review 2019年第6期6卷 1176-1188页

作者： Yuxin Chen Yang Shen Pei Lin Ding Tong Yixin Zhao Stefano Allesina Xu Shen Chung-I Wu School of Life Science Sun Yat-Sen University Target Discovery Research Boehringer Ingelheim Pharma Gmb H & Co KG Department of Biostatistics School of Public Health Yale University Department of Ecology and Evolution University of Chicago

Food web and gene regulatory networks(GRNs) are large biological networks, both of which can be analyzed using the May–Wigner theory. According to the theory, networks as large as mammalian GRNs would require dedicated gene products for stabilization. We propose that micro rnas(mi rnas) are those products. More than 30% of genes are repressed by mi rnas, but most repressions are too weak to have a phenotypic consequence. The theory shows that(i) weak repressions cumulatively enhance the stability of GRNs, and(ii) broad and weak repressions confer greater stability than a few strong ones. Hence, the diffuse actions of mi rnas in mammalian cells appear to function mainly in stabilizing GRNs. The postulated link between m RNA repression and GRN stability can be seen in a different light in yeast, which do not have mi rnas. Yeast cells rely on non-specific RNA nucleases to strongly degrade m rnas for GRN stability. The strategy is suited to GRNs of small and rapidly dividing yeast cells, but not the larger mammalian cells. In conclusion, the May–Wigner theory, supplanting the analysis of small motifs, provides a mathematical solution to GRN stability, thus linking mi rnas explicitly to ’developmental canalization’.

关键词： micro rnas network stability canalization May–Wigner theory systems biology RNA crosstalk

Noncoding rnas as drivers of the phenotypic plasticity of oesophageal mucosa

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World Journal of Gastroenterology 2017年第43期23卷 7653-7656页

作者： Matteo Fassan Sonia Facchin Giada Munari Giuseppe Nicolò Fanelli Greta Lorenzon Edoardo Savarino Surgical Pathology Unit Department of MedicineUniversity of Padua Gas-troenterology Unit Department of Surgical Oncology and GastroenterologyUniversity of Padua

The histological commitment of the lower oesophageal mucosa largely depends on a complex molecular landscape. After extended inflammatory insult due to gastroesophageal reflux disease,squamous oesophageal mucosa may differentiate into columnar metaplastic mucosa. In this setting,the presence of intestinal metaplasia is considered the starting point of Barrett's carcinogenetic cascade. Aside from secondary prevention strategies for Barrett's mucosa(BM) patients,there are multiple endoscopic ablative therapies available for BM eradication and for the replacement of metaplastic epithelia with a neosquamous mucosa. However,BM frequently recurs in a few years,which supports the notable phenotypic plasticity of the oesophageal mucosa. In recent years,several reports pinpointed a class of small noncoding rnas,the micro rnas(mi rnas),as principal effectors and regulators of oesophageal mucosa metaplastic(and neoplastic) transformation. Because of mi rnas notable stability in fixed archival diagnostic specimens,expression profiling of mi rnas represent an innovative diagnostic,prognostic and predictive tool in the stratification of phenotypic alterations in the oesophageal mucosa.

关键词： Barrett's mucosa Biomarkers Noncoding rnas micro rnas Metaplasia

Role of Dicer on tumorigenesis in glioma cells

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Neural Regeneration Research 2010年第17期5卷 1324-1329页

作者： Anling Zhang Lei Han Guangxiu Wang Zhifan Jia Peiyu Pu Chunsheng Kang Department of Neurosurgery Tianjin Medical University General Hospital and Laboratory of Neuro-OncologyTianjin Neurological InstituteTianjin 300052China

micro rnas（mirnas）are non-coding,single-stranded rnas that regulate target gene expression by repressing translation or promoting RNA *** studies show that miRNA expression is globally decreased in some human *** is an essential component of the miRNA processing *** determine whether global reduction of miRNA effects tumorigenesis,small interfering RNA were designed to target Dicer to restrain whole miRNA expression in the glioblastoma cell *** effective knock-down of Dicer,tumor cells were invasive and proliferative,and globally impaired miRNA processing enhanced proliferation and invasiveness of glioma cells in *** of Dicer expression resulted in a more aggressive glioma phenotype,which suggests that global reduction of miRNA expression could have an oncogenic role in glioblastoma cells.

关键词： Dicer glioma tumorigenesis small interfering RNA micro rnas

Gene regulatory networks in atrial fibrillation

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World Journal of Medical Genetics 2016年第1期6卷 1-16页

作者： Diego Franco Estefanía Lozano-Velasco Amelia Aranega Cardiovascular Development Group Department of Experimental Biology B3-362Faculty of Experimental SciencesUniversity of Jaén

Atrial fibrillation(AF)is the most frequent arrhythmogenic syndrome in *** an estimate incidence of1%-2%in the general population,AF raises up to almost10%-12%in 80+***,AF represents nowadays a highly prevalent medical problem generating a large economic *** the electrophysiological level,distinct mechanisms have been ***,despite its prevalence,the genetic and molecular culprits of this pandemic cardiac electrophysiological abnormality have remained largely *** genetics of AF familiar cases have demonstrated that single nucleotide mutations in distinct genes encoding for ion channels underlie the onset of AF,albeit such alterations only explain a minor subset of patients with *** recent years,analyses by means of genome-wide association studies have unraveled a more complex picture of the etiology of AF,pointing out to distinct cardiac-enriched transcription factors,as well as to other regulatory *** a new layer of regulatory mechanisms have emerged,i.e.,post-transcriptional regulation mediated by non-coding RNA,which have been demonstrated to exert pivotal roles in cardiac *** this manuscript,we aim to provide a comprehensive review of the genetic regulatory networks that if impaired exert electrophysiological abnormalities that contribute to the onset,and subsequently,on self-perpetuation of AF.

关键词： Atrial fibrillation Genetics micro rnas Genome-wide association studies PITX2

Can epigenetic and inflammatory biomarkers identify clinically aggressive prostate cancer?

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World Journal of Clinical Oncology 2020年第2期11卷 43-52页

作者： Pedro Bargao Santos Hitendra Patel Rui Henrique Ana Felix Department of Urology Prof.Doutor Fernando Fonseca HospitalAmadora 2720-276Portugal Department of Urology University Hospital North NorwayTromsø9019Norway Department of Urology St George’s University HospitalsTootingLondon SW170QTUnited Kingdom Departments of Pathology and Cancer Biology and Epigenetics Group-Research Center Portuguese Oncology Institute of PortoPorto 4200-072Portugal Department of Pathology and Molecular Immunology Institute of Biomedical Sciences Abel SalazarUniversity of PortoPorto 4099-002Portugal Department of Pathology Portuguese Oncology Institute of LisbonLisbon 1099-023Portugal Department of Pathology NOVA Medical SchoolLisbon 1169-056Portugal

Prostate cancer(PCa)is a highly prevalent malignancy and constitutes a major cause of cancer-related morbidity and *** emerges through the acquisition of genetic and epigenetic *** modifications include DNA methylation,histone modifications and micro RNA *** generate heritable transformations in the expression of genes but do not change the DNA *** in DNA methylation(hypo and hypermethylation)are the most characterized in *** lead to genomic instability and inadequate gene *** and minor-specific modifications in chromatin recasting are involved in PCa,with signs suggesting a dysfunction of enzymes modified by *** RNA deregulation also contributes to the initiation of PCa,including involvement in androgen receptor signalization and *** influence of inflammation on prostate tumor carcinogenesis is currently much better *** discoveries about microbial species resident in the urinary tract suggest that these are the initiators of chronic inflammation,promoting prostate inflammatory atrophy and eventually leading to *** characterization of the relationship between the urinary microbiome and prostatic chronic inflammation will be crucial to develop plans for the prevention of *** prevalent nature of epigenetic and inflammatory alterations may provide potential biomarkers for PCa diagnosis,treatment decisions,evaluation of prognosis and posttreatment surveillance.

关键词： Prostate cancer Epigenetics DNA methylation Histone modifications micro rnas Inflammation Initiation and progression Prognosis

microRNA-34a在肝病发生发展中的作用

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中华中医药学刊 2022年第11期40卷 205-209页

作者：丘佩容邓鑫陈泽山文彬王淼东朱文琳徐艺倪广西中医药大学广西南宁530001 广西中医药大学附属瑞康医院广西南宁530001 钦州市中医医院广西钦州535000

microRNA是一种小的非编码RNA,通过对各种靶基因的负调控参与细胞的增殖和凋亡等环节。目前越来越多证据表明,microRNA-34a参与非酒精性脂肪性肝病(non-alcoholic fatty liver disease, NAFLD)、酒精性肝病(alcoholic liver disease, A... 详细信息

microRNA是一种小的非编码RNA,通过对各种靶基因的负调控参与细胞的增殖和凋亡等环节。目前越来越多证据表明,microRNA-34a参与非酒精性脂肪性肝病(non-alcoholic fatty liver disease, NAFLD)、酒精性肝病(alcoholic liver disease, ALD)、肝纤维化等多种肝脏疾的发生发展过程,尤其在肝癌方面发挥着重要作用;此外,中药单体或中药复方可以通过microRNA-34a对肝脏疾病进行调节。microRNA-34a作为无创生物学标志物为肝脏疾病的诊疗提供参考。

关键词： micro rnas micro RNA-34a 肝脏疾病

3,6-Dihydroxyflavone Suppresses Breast Carcinogenesis by Epigenetically Regulating miR-34a and miR-21

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3,6-Dihydroxyflavone Suppresses Breast Carcinogenesis by Epi...