检索结果-南通市图书馆

mi R-382 functions as a tumor suppressor against esophageal squamous cell carcinoma

World Journal of Gastroenterology 2017年第23期23卷 4243-4251页

作者： Jie Feng Bo Qi Ling Guo Ling-Yun Chen Xiu-Feng Wei Yu-Zhen Liu Bao-Sheng Zhao Department of Thoracic Surgery The First Affiliated Hospital of Xinxiang Medical University Department of Operating Room The First Affiliated Hospital of Xinxiang Medical University

AIM To explore the effect of mir-382 on esophageal squamous cell carcinoma (ESCC) in vitro and its possible molecular mechanism. METHODS Eca 109 cells derived from human ESCC and Het-1A cells derived from human normal esophageal epithelium were used. Lentivirus-mediated mir-382 was overexpressed in Eca109 cells. The effect of mir-382 on cell proliferation was evaluated by MTT and colony formation assay. For cell cycle analysis, cells were fixed and stained for 30 min with propidium iodide (PI) staining buffer containing 10 mg/mL PI and 100 mg/mL RNase A, and analyzed by BD FACSCalibur (TM) flow cytometer. For cell apoptosis assay, cells were stained with an Annexin V-FITC/PI Apoptosis Detection Kit according to the manufacturer's instructions and analyzed by a dual-laser flow cytometer. Cell invasion and migration abilities were determined through use of transwell chambers, non-coated or pre-coated with matrigel. Levels of proteins related to cell growth and migration were examined by western blotting. RESULTS Endogenous mir-382 was down-regulated in Eca109 cells compared with Het-1A. Introduction of mir-382 not only significantly inhibited proliferation and colony formation, but also arrested cell cycle at the G2/M phase, as well as promoted apoptosis and autophagy in Eca109 cells. Migration, invasion and epithelial-mesenchymal transition of Eca109 cells were suppressed by overexpressing mir-382. Western blotting results showed that mir-382 inhibited the phosphorylation of mTOR and 4E-BP1. CONCLUSION mir-382 functions as a tumor suppressor against ESCC development and metastasis, and could be considered as a potential drug source for the treatment of ESCC patients.

关键词： mir-382 Esophageal squamous cell carcinoma Tumor suppressor Proliferation Migration Invasion

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Downregulation of microRNA-382 is associated with poor outcome of esophageal squamous cell carcinoma

引用

世界胃肠病学杂志：英文版 2015年第22期21卷 6884-6891页

作者： Bo Qi Jian-Guo Lu Wen-Jian Yao Ting-Min Chang Xiu-Guang Qin Ying-Hua Ji Tian-Yun Wang Shang-Guo Liu Han-Chen Li Yu-Zhen Liu Bao-Sheng Zhao Department of Thoracic Surgery The First Affiliated Hospital of Xinxiang Medical University Department of Gastroenterology The First Affiliated Hospital of Xinxiang Medical University Department of Oncology The First Affiliated Hospital of Xinxiang Medical University Department of Molecular Biology Xinxiang Medical University

AIM: To study the potential prognostic role of microRNA-382(mir-382) in esophageal squamous cell carcinoma(ESCC).METHODS: Forty six patients were divided into 2groups according to postoperative survival time:the poor outcome group(28 patients), who showed early metastasis but no recurrence, and died within 1year after surgery, 12 patients of the group received postoperative chemotherapy treatment that was given after early metastasis happening; the good outcome group(18 patients), who had no clinical metastasis and recurrence, and survived 5 years or more after surgery, all patients did not receive any postoperative treatment. Total RNA was extracted from the patients' formalin-fixed and paraffin-embedded esophageal cancer tissues. mir-382 level was evaluated using highthroughput real-time quantitative polymerase chain reaction analysis. The correlation between mir-382 level and clinicopathologic features was analyzed through COX regression model, and Kaplan-Meier analysis was used to analyze the relationship betweenmir-382 level and patient survival ***: mir-382 was differentially expressed in the two groups. Overall the average mir-382 level in the ESCC patients with good outcome was 9.8 ± 3.8,while mir-382 level in the ESCC patients with poor outcome was 3.0 ± 0.8. The differences of mir-382 levels between two groups were significant(P mir-382 expression level generally had a significant reversecorrelation with ESCC patient survival time(P mir-382 had a longer survival time either among individuals with the same tumor stage or among the overall ***: mir-382 levels are reverse-correlated with ESCC poor outcomes, suggesting that mir-382 could be a potential predictive biomarker for both prognosis and treatment of ESCC.

关键词： Esophageal squamous cell carcinoma mir-382 Metastasis Outcome Prognosis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：