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Cancer Communications 2024年第2期44卷 282-286页

作者： Bianca Cesaro Alessia Iaiza Fabio Piscopo Marco Tarullo Eleonora Cesari Dante Rotili Antonello Mai Alberto Diana Michela Londero Luca Del Giacco Riccardo Masetti Alba Di Leone Chiara Naro Silvia Masciarelli Giulia Fontemaggi Claudio Sette Francesco Fazi Alessandro Fatica Department of Anatomical HistologicalForensic&Orthopedic SciencesSection of Histology&Medical EmbryologySapienza University of RomeRomeItaly Department of Biology and Biotechnologies“Charles Darwin” Sapienza University of RomeRomeItaly GSTeP Organoids Research Core Facility Fondazione Policlinico A.Gemelli Istituto di Ricovero e Cura a Carattere Scientifico(IRCCS)RomeItaly Department of Drug Chemistry and Technologies Sapienza University of RomeRomeItaly Pasteur Institute Cenci-Bolognetti FoundationSapienza University of RomeRomeItaly Department of BioSciences University of MilanMilanItaly Multidisciplinary Breast Center Dipartimento Scienze della Salute della Donna e del Bambino e di SanitàPubblicaCatholic University of the Sacred HeartRomeItaly Department of Neuroscience Section of Human AnatomyCatholic University of the Sacred HeartRomeItaly Oncogenomic and Epigenetic Unit IRCCS Regina Elena National Cancer InstituteRomeItaly

Dear Editor,N 6-methyladenosine(m^(6)A)is a critical mRNA modi-fication catalyzed by the enzyme methyltransferase-like 3(METTL3),with implications in RNA metabolism.METTL3 upregulation is associated with cancer progre... 详细信息

关键词： cancer methyltransferase metastasis

PHF6 functions as a tumor suppressor by recruiting methyltransferase SUV39H1 to nucleolar region and offers a novel therapeutic target for PHF6-muntant leukemia

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Acta Pharmaceutica Sinica B 2022年第4期12卷 1913-1927页

作者： Hsiang-i Tsai Yanping Wu Rui Huang Dandan Su Yingyi Wu Xiaoyan Liu Linglu Wang Zhanxue Xu Yuxin Pang Chong Sun Chao He Fan Shu Haitao Zhu Dongqing Wang Fang Cheng Laiqiang Huang Hongbo Chen School of Pharmaceutical Sciences(Shenzhen) Sun Yat-sen UniversityShenzhen 518107China Department of Medical Imaging the Affiliated Hospital of Jiangsu UniversityZhenjiang 212001China Department of Hematology Zhujiang HospitalSouthern Medical UniversityGuangzhou 510000China The Shenzhen Key Lab of Gene and Antibody Therapy Center for Biotechnology&BiomedicineDivision of Life and Health SciencesGraduate School at ShenzhenTsinghua UniversityShenzhen 518055China School of Traditional Medicine Materials Resource Guangdong Pharmaceutical UniversityYunfu 527322China Immune Regulation in Cancer German Cancer Research CenterHeidelberg 69120Germany

Mutations in the plant homeodomain-like finger protein 6(PHF6)gene are strongly associated with acute myeloid(AML)and T-cell acute lymphoblastic leukemia(T-ALL).In this study,we demonstrated that PHF6 can bind to H3K9me3 and H3K27me1 on the nucleolar chromatin and recruit histone methyltransferase SUV39H1 to the rDNA locus.The deletion of PHF6 caused a decrease in the recruitment of SUV39H1 to rDNA gene loci,resulting in a reduction in the level of H3K9me3 and the promotion of rDNA transcription.The knockdown of either SUV39H1 or PHF6 significantly attenuated the effects of increase in H3K9me3 and suppressed the transcription of rDNA induced by the overexpression of the other interacting partner,thereby establishing an interdependent relationship between PHF6 and SUV39H1 in their control of rRNA transcription.The PHF6 clinical mutants significantly impaired the ability to bind and recruit SUV39H1 to the rDNA loci,resulting in an increase in rDNA transcription activity,the proliferation of in vitro leukemia cells,and the growth of in vivo mouse xenografts.Importantly,significantly elevated levels of pre-rRNA were observed in clinical AML patients who possessed a mutated version of PHF6.The specific rDNA transcription inhibitor CX5461 significantly reduced the resistance of U937 AML cells deficient in PHF6 to cytarabine,the drug that is most commonly used to treat AML.Collectively,we revealed a novel molecular mechanism by which PHF6 recruits methyltransferase SUV39H1 to the nucleolar region in leukemia and provided a potential therapeutic target for PHF6-mutant leukemia.

关键词： PHF6 SUV39H1 AML rDNA transcription Epigenetic CX5461 methyltransferase Leukemia

Engineered geranyl diphosphate methyltransferase produces 2-methyl-dimethylallyl diphosphate as a noncanonical C_(6) unit for terpenoid biosynthesis

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Synthetic and Systems Biotechnology 2023年第1期8卷 107-113页

作者： Chen-Yang Xia Bo-Wen Lu Ji-Yun Cui Bai-Yang Wang Yue-Yang Sun Fei Gan Hubei Key Laboratory of Cell Homeostasis College of Life ScienceWuhan UniversityWuhanChina TaiKang Center for Life and Medical Science Wuhan UniversityWuhanChina Hubei Provincial Cooperative Innovation Center of Industrial Fermentation WuhanChina

Terpenoids constitute the largest class of natural products with complex structures,essential functions,and versatile applications.Creation of new building blocks beyond the conventional five-carbon(C_(5))units,dimethylallyl diphosphate(DMAPP)and isopentenyl diphosphate,expands significantly the chemical space of terpenoids.Structure-guided engineering of an S-adenosylmethionine-dependent geranyl diphosphate(GPP)C2-methyltransferase from Streptomyces coelicolor yielded variants converting DMAPP to a new C_(6) unit,2-methyl-DMAPP.Mutation of the Gly residue at the position 202 resulted in a smaller substrate-binding pocket to fit DMAPP instead of its native substrate GPP.Replacement of Phe residue at the position 222 with a Tyr residue contributed to DMAPP binding via hydrogen bond.Furthermore,using Escherichia coli as the chassis,we demonstrated that 2-methyl-DMAPP was accepted as a start unit to generate noncanonical trans-and cis-prenyl diphosphates(C_(5n+1))and terpenoids.This work provides insights into substrate recognition of prenyl diphosphate methyltransferases,and strategies to diversify terpenoids by expanding the building block portfolio.

关键词： Protein engineering Terpenoid biosynthesis methyltransferase Noncanonical building block

Arsenic biotransformation and an arsenite Sadenosylmethionine methyltransferase in plankton

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Journal of Environmental Sciences 2017年第11期29卷 118-121页

作者： Jagdeesh S.Uppal Qin Shuai Zhuang Li X.Chris Le Division of Analytical and Environmental Toxicology Department of Laboratory Medicine and Pathology Faculty of Medicine and DentistryUniversity of Alberta College of Material Science and Chemistry China University of GeosciencesWuhan China

Arsenic（As）is a well-recognized toxicant and carcinogen.Chronic exposure to inorganic arsenic causes a range of human cancers（e.g.,skin,bladder,and lung）and increases the risk of developing diabetes,hypertension,and cardiovascular and neurological diseases.The prevalence of arsenic species and the severity of their health effects continue to drive and demand for extensive research（Carlin et al.,2016）.

关键词： Arsenic speciation Spirulina platensis methyltransferase Arsenic detoxification Biomethylation enzymes Aquatic environment

Overexpression of INCREASED CAMBIAL ACTIVITY, a putative methyltransferase, increases cambial activity and plant growth

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Journal of Integrative Plant Biology 2016年第11期58卷 874-889页

作者： Hyunsook Kim Mikiko Kojima Daeseok Choi Soyoung Park Minami Matsui Hitoshi Sakakibara Ildoo Hwang Department of Life Sciences Pohang University of Science and Technology Pohang 790-784 Korea Synthetic Genomics Research Team Biomass Research Cooperation Division (BMEP) RIKEN Center for Sustainable Resource Science (CSRS) Tsurumi Yokohama 230-0045 Japan Riken Center for Sustainable Resource Science (CSRS) Tsurumi Yokohama 230-0045 Japan

Cambial activity is a prerequisite for secondary growth in plants; however, regulatory factors controlling the activity of the secondary meristem in radial growth remain elusive. Here, we identified INCREASED CAMBIAL ACTIVITY （ICA）, a gene encoding a putative pectin methyltransferase, which could function as a modulator for the meristematic activity of fascicular and interfascicular cambium in Arabidopsis. An overexpressing transgenic line, 35S：＇1CA, showed accelerated stern elongation and radial thickening, resulting in increased accumulation of biomass, and increased levels of cytokinins （CKs） and gibberellins （GAs）. Expression of genes encoding pectin methylesterases involved in pectin modification together with pectin methyltransferases was highly induced in 355：：ICA, which might contribute to an increase of methanol emission as a byproduct in 35S：ICA. Methanol treatment induced the expression of GA- or CK-responsive genes and stimulated plant growth. Overall, we propose that ectopic expression of ICA increases cambial activity by regulating CK and GA homeostasis, and methanol emission, eventually leading to stem elongation and radial growth in the inflorescence stem.

关键词： Cambial activity cytokinin gibberellin methanol methyltransferase pectin shoot growth

O^6-METHYLGUANINE-DNA methyltransferase AND HUMAN CANCER CHEMOTHERAPY

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Science China Chemistry 1991年第6期34卷 675-682页

作者：章扬培王德文陈月能吴英徐惠英范国才白晓彬王晓明李洁珣朱力 Institute of Radiation Medicine Academy of Military Medical Sciences Beijing 100850 PRC Cancer Institute Chinese Academy of Medical Sciences Beijing 100021 PRC

Two kinds of human tumor cell strains having different activity of O^6-methylguanine-DNA methyltransferase (O^6-MT) were transplanted into nude mice. Then the mice were inject-ed intraperitoneally with bifunctional alkylating agent 1--(4--amino--2-methyl--5--pyrimidinyl)methy1-3-(2-chloroethyl)-3-nitrosourea hydrochloride (ACNU). The tumors with low O^6-MTactivity were quickly suppressed or cured. The result suggests that some tumors, if previ-sionally determined with low O^6-MT activity, might be efficiently cured by treatment withACNU. This probably opens a new way for human cancer chemotherapy.

关键词： O^6-methylguanine-DNA methyltransferase ACNU HeLaS3 cells HeLaMR cells

Functional analysis of the methyltransferase SMYD in the single-cell model organism Tetrahymena thermophila

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Marine Life Science & Technology 2020年第2期2卷 109-122页

作者： Xiaolu Zhao Yuan Li Lili Duan Xiao Chen Fengbiao Mao Mina Juma Yifan Liu Weibo Song Shan Gao Institute of Evolution and Marine Biodiversity Ocean University of ChinaQingdao 266003China Department of Pathology University of Michigan.Ann Arbor.MI 48109USA Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and TechnologyQingdao 266003China

Lysine methylation of histones and non-histones plays a pivotal role in diverse cellular processes.The SMYD(SET and MYND domain)family methyltransferases can methylate various histone and non-histone substrates in mammalian systems,implicated in HSP90 methylation,myofilament organization,cancer inhibition,and gene transcription regulation.To resolve controversies concerning SMYD's substrates and functions,we studied SMYD1(TTHERM_00578660),the only homologue of SMYD in the unicellular eukaryote Tetrahymena thermophila.We epitope-tagged SMYD1,and analyzed its localization and interactome.We also characterized △SMYD1 cells,focusing on the replication and transcription phenotype.Our results show that:(1)SMYD1 is present in both cytoplasm and transcriptionally active macronucleus and shuttles between cytoplasm and macronucleus,suggesting its potential association with both histone and non-histone substrates;(2)SMYD1 is involved in DNA replication and regulates transcription of metabolism-related genes;(3)HSP90 is a potential substrate for SMYD1 and it may regulate target selection of HSP90,leading to pleiotropic effects in both the cytoplasm and the nucleus.

关键词： Tetrahymena thennophila methyltransferase SMYD DNA replication Transcription HSP90

Flavivirus RNA cap methyltransferase:structure,function,and inhibition

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Frontiers in Biology 2010年第4期5卷 286-303页

作者： Lihui LIU Hongping DONG Hui CHEN Jing ZHANG Hua LING Zhong LI Pei-Yong SHI Hongmin LI Wadsworth Center New York State Department of Health120 New Scotland AveAlbanyNY 12208USA Novartis Institute for Tropical Diseases 10 Biopolis RoadSingapore 138670 Department of Biomedical Sciences School of Public HealthState University of New YorkAlbanyNew York 12201-0509USA

Many flaviviruses are significant human pathogens.The plus-strand RNA genome of a flavivirus contains a 5′terminal cap 1 structure(m7GpppAmG).The flavivirus encodes one methyltransferase(MTase),located at the Nterminal portion of the NS5 RNA-dependent RNA polymerase(RdRp).Here we review recent advances in our understanding of flaviviral capping machinery and the implications for drug development.The NS5 MTase catalyzes both guanine N7 and ribose 2'-OH methylations during viral cap formation.Representative flavivirus MTases,from dengue,yellow fever,and West Nile virus(WNV),sequentially generate GpppA!m7GpppA!m7GpppAm.Despite the existence of two distinct methylation activities,the crystal structures of flavivirus MTases showed a single binding site for S-adenosyl-L-methionine(SAM),the methyl donor.This finding indicates that the substrate GpppA-RNA must be repositioned to accept the N7 and 2'-O methyl groups from SAM during the sequential reactions.Further studies demonstrated that distinct RNA elements are required for the methylations of guanine N7 on the cap and of ribose 2'-OH on the first transcribed nucleotide.Mutant enzymes with different methylation defects can trans complement one another in vitro,demonstrating that separate molecules of the enzyme can independently catalyze the two cap methylations in vitro.In the context of the infectious virus,defects in both methylations,or a defect in the N7 methylation alone,are lethal to WNV.However,viruses defective solely in 2'-O methylation are attenuated and can protect mice from later wild-typeWNV challenge.The results demonstrate that the N7 methylation activity is essential for the WNV life cycle and,thus,methyltransferase represents a novel and promising target for flavivirus therapy.

关键词： Flavivirus NS5 RNA cap methylation methyltransferase structure and function inhibitor

Sensitive detection of DNA methyltransferase activity based on rolling circle amplification technology

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Chinese Chemical Letters 2014年第7期25卷 1047-1051页

作者： Pei Liu Xiao-Hai Yang Qing Wang Jing Huang Jian-Bo Liu Ying Zhu Lei-Liang He Ke-Min Wang State Key Laboratory of Chemo/Biosensing and Chemometrics College of Chemistry and Chemical EngineeringKey Laboratory for Bio-nanotechnology and Molecular Engineering of Hunan ProvinceHunan University

This work develops a fluorescence approach for sensitive detection of DNA methyltransferase activity based on endonuclease and rolling circle amplification （RCA） technique. In the presence of DNA adenine methylation （Dam） MTase, the methylation-responsive sequence of hairpin probe is methylated and cleaved by the methylation-sensitive restriction endonuclease Dpn 1. The products cleaved by restriction endonuclease Dpn I then function as a signal primer to initiate RCA reaction by hybridizing with the circular DNA template. Each RCA product containing thousands of repeated sequences might hybridize with a large number of molecular beacons （detection probes）, resulting in an enhanced fluorescence signal. In the absence of Dam MTase, neither methylation/cleavage nor RCA reaction can be initiated and no fluorescence signal is observed. The proposed method exhibits a dynamic range from 0.5 U/mL to 30 U/mL and a detection limit of 0.18 U/mL. This method can be used for the screening of antimicrobial drugs and has a great potential to be further applied in early clinical diagnosis.

关键词： methyltransferase RCAD am MTase Dpn 1 Fluorescence

Development of a rapid and sensitivity magnetic chemiluminescence immunoassay for DNA methyltransferase 1 in human serum

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Chinese Chemical Letters 2019年第5期30卷 1031-1034页

作者： Sitian He Leiliang He Beibei Liu Songcheng Yu Li'e Liu Yongmei Tian Jia Wang Lihua Ding Yilin Wang Lingbo Qu Fei Yu Yongjun Wu Collegeof Public Health Zhengzhou UniversityZhengzhou 450001China College of Chemistry and Molecular Engineering Zhengzhou UniversityZhengzhou 450001China Henan Joint International Research Laboratory of Green Construction of Functional Molecules and Their Bioanalytical Applications Zhengzhou 450001China

DNA methyltransferase 1(DNMT1)is a useful biomarker for lung cancer in early clinical diagnosis.A rapid magnetic chemiluminescence immunoassay(MCLIA)for DNMT1 in human serum has been developed.Horseradish peroxidase(HRP)-second-Ab was used to labeled polyclonal antibodies of anti-DNMT1.DNMT1 in sample integrates with specific immunomagnetic beads and can constitute a supersandwiched immunoreaction.In magnetic field,nonspecific materials can be separated.After luminescent substrate luminol-H2O2-BIP was added,the relative light unit(RLU)of HRP was detected and was discovered to be directly proportional to the content of DNMT1 in sample.The correlative variables involved in the MCLIA value were optimized and the methodological evaluation was carried out.After optimization,in the range of0.5–128 ng/mL,the linear regression equation was y=0.5014 x+1.769(x was logCDNMT1,y was relative luminescence units(RLU)/RLU0),and the limit of detection was 0.01 ng/mL.The RSD of intra-and interassays were 15.8%–16.9%and 14.3%–18.1%,respectively.The recovery was from 70.0%to 106.2%.Furthermore,paralleled with purchasable enzyme-linked immunosorbent assay(ELISA)kits,MCLEIA had lower detection limit,wider linear range and shorter detection time.Therefore,the MCLEIA established in this study could be used for the sensitive detection of DNMT1 in serum sample.

关键词： DNA methyltransferase 1 Biomarker Chemiluminescence immunoassay Magnetic particles Human serum