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Journal of Integrative Plant Biology 2019年第2期61卷 120-139页

作者： Qiang-Qiang Zhao Rong-Nan Lin Lin Li She Chen Xin-Jian He National Institute of Biological Sciences Graduate School of Peking Union Medical College

Although the mechanism of DNA methylationmediated gene silencing is extensively studied, relatively little is known about how promoter methylated genes are protected from transcriptional silencing. SUVH1, an Arabidopsis Su(var)3-9 homolog, was previously shown to be required for the expression of a few promoter methylated genes. By chromatin immunoprecipitation combined with sequencing, we demonstrate that SUVH1 binds to methylated genomic loci targeted by RNA-directed DNA methylation. SUVH1 and its homolog SUVH3 function partially redundantly and interact with three DNAJ domain-containing homologs, SDJ1, SDJ2, and SDJ3, thus forming a complex which we named SUVH-SDJ. The SUVH-SDJ complex components are co-localized in a large number of methylated promoters and are required for the expression of a subset of promoter methylated genes. We demonstrate that the SUVHSDJ complex components have transcriptional activation activity. SUVH1 and SUVH3 function synergistically with SDJ1,SDJ2, and SDJ3 and are required for plant viability. This study reveals how the SUVH-SDJ complex protects promoter methylated genes from transcriptional silencing and suggests that the transcriptional activation of promoter methylated genes mediated by the SUVH-SDJ complex may play a critical role in plant growth and development.

关键词： A methylated-DNA-binding complex required for plant development mediates transcriptional activation of promoter methylated genes Figure DNA

Clinical value of preoperative methylated septin 9 in Chinese colorectal cancer patients

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World Journal of Gastroenterology 2019年第17期25卷 2099-2109页

作者： Xue Yang Zhi-Jie Xu Xi Chen Shuang-Shuang Zeng Long Qian Jie Wei Mei Peng Xiang Wang Wan-Li Liu Hong-Ying Ma Zhi-Cheng Gong Yuan-Liang Yan Department of Pharmacy Institute for Rational and Safe Medication PracticesNational Clinical Research Center for Geriatric DisordersXiangya HospitalCentral South University Department of Pathology Xiangya HospitalCentral South University

BACKGROUND The methylated septin 9(mSEPT9) assay was the first blood-based test approved by the United States Food and Drug Administration as a colorectal screening ***, the diagnostic and prognostic role of preoperative mSEPT9 for colorectal cancer(CRC) in Chinese patients is still *** To improve the understanding of diagnostic and prognostic factors, serum m SEPT9 was detected in Chinese CRC *** A retrospective analysis of 354 cases, of which 300 had CRC and 54 were normal,was performed in China. Patients' characteristics, treatments, and laboratory data, including age, the date of surgery, Union for International Cancer Control(UICC) stages, distant metastasis(M), and so on, were collected. Methylation levels of SEPT9 were quantified by quantitative, methylation-specific polymerase chain reaction before surgery. In addition, the effects of mSEPT9 on the occurrence and prognosis of 330 CRC cases from The Cancer Genome Atlas(TCGA) database were evaluated using bioinformatics analyses. Potential prognostic factors for overall survival(OS) and progression-free survival(PFS)were evaluated by Kaplan-Meier univariate *** Chinese CRC patients, positive mSEPT9 was strongly associated with advanced UICC stages, deeper invasion by the primary tumor, and more distant metastasis. Methylation levels of SEPT9 were stage-dependent and showed a stepwise increase in UICC stages(I–IV), primary tumor categories(T1–T4),regional node categories(N0–N2), and distant metastasis categories(M0–M1).The patients with positive mSEPT9 showed a tendency toward lower PFS. After analyzing TCGA clinical data, the high mSEPT9 group was found to be obviously correlated only with more distant metastasis. The patients with high mSEPT9 levels showed a tendency toward lower OS. Besides, nine meaningful mSEPT9 sites were found to provide guidance for the follow-up *** MSEPT9 analysis may add valuable information to current tumor staging. Serum m

关键词： methylated septin 9 methylated Colorectal cancer Diagnosis Prognosis

The relationship between methylated chromans and maturity of organic matter in the source rocks from Jianghan hypersaline basin

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Science China Earth Sciences 2009年第S1期52卷 34-41页

作者： BAO JianPing1,ZHU CuiShan1 & MA AnLai2 1 Key Laboratory of Oil & Gas Resource and Exploration Technology,Ministry of Education,Geochemistry Department,Yangtze University,Jingzhou 434023,China 2 Institute of Petroleum Exploration and Development,Sinopec,Beijing 100083,China

The distribution,the relative composition,and their variation of methylated chromans(MTTC) with maturation of organic matter in the source rocks from Mingjia 1 well,Jianghan hypersaline basin,have been analyzed and studied. There is a close relationship between the distribution of methylatd chromans and paleosalinity of depositional environment. In the Eq1-Eq3 source rocks deposited under hypersaline environment with lower Pr/Ph ratio,higher gammacerane index and abundant organic sulfur-containing compounds,methyl MTTC and dimethyl MTTC are main components,but in the Eq4 source rocks deposited non-hypersaline environment with relatively higher Pr/Ph ratio,lower gammacerane index and organic sulfur-containing compounds,trimethyl MTTC is a major compound in methylate chromans. Obviously,the distributions and the compositions of methylated chromans in the source rocks are in harmony with biomarker indicators indicating the paleaosalinity and redox of depositional environment. The relationship between the ratio of 5,8-dimethyl MTTC to 7,8-dimethyl MTTC(dimethyl MTTC ratio) and the maturity of organic matter in the source rocks from Mingjia 1 well has been discussed. The results show that dimethyl MTTC ratio increased with the burial depth and the maturation of organic matter in the source rocks,especially in immature level. It is noteworthy that this ratio is very susceptible to small variation in the maturity of organic matter in the source rocks when vitrinite reflectance Ro is less than 0.65%. It may imply that this ratio is a good and susceptible aromatic maturity indicator for the relative maturity of organic matter in immature source rocks.

关键词： methylated chromans dimethyl MTTC ratio source rock Jianghan hypersaline basin depositional environment maturity of organic matter

Genome-wide differences in DNA methylation changes in caprine ovaries between oestrous and dioestrous phases

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Journal of Animal Science and Biotechnology 2019年第1期10卷 25-32页

作者： Xiaopeng An Haidong Ma Peng Han Chao Zhu Binyun Cao Yueyu Bai College of Animal Science and Technology Northwest A&F University Animal Health Supervision Institute of Henan Province

Background: DNA methylation plays a vital role in *** genome DNA methylation changes during the oestrous phase(ES) and dioestrous phase(DS) in the ovaries of Guanzhong dairy goats were investigated using bisulphite sequencing to understand the molecular biological mechanisms of these goats' oestrous ***: We discovered distinct genome-wide DNA methylation patterns in ES and DS ovaries.A total of 26,910 differentially methylated regions were upregulated and 21,453 differentially methylated regions were downregulated in the ES samples compared with the DS samples(P-values ≤0.05 and fold change of methylation ratios ≥2).Differentially methylated region analysis showed hypomethylation in the gene body regions and hypermethylation in the joining region between upstream regions and gene *** methylation ratios of the STAR,FGF2,FGF12,BMP5 and SMAD6 genes in the ES samples were lower than those of the DS samples(P-values≤0.05 and fold change of methylation ratios ≥2).Conversely,the methylation ratios of the EGFR,TGFBR2,IGF2 BP1 and MMD2 genes increased in the ES samples compared with the DS *** addition,223 differentially methylated genes were found in the GnRH signalling pathway(KO04912),ovarian steroidogenesis pathway(KO04913),oestrogen signalling pathway(KO04915),oxytocin signalling pathway(KO04921),insulin secretion pathway(KO04911) and MAPK signalling pathway(KO04010).Conclusions: This study is the first large-scale comparison of the high-resolution DNA methylation landscapes of oestrous and dioestrous ovaries from dairy *** studies and our investigations have shown that the NR5 A2,STAR,FGF2 and BMP5 genes might have potential application value in regulating caprine oestrus.

关键词： Differentially methylated genes Gene ontology Oestrous cycle Pathway

Influence of Adjuvants on the Efficacy of Tolpyralate plus Atrazine for the Control of Annual Grass and Broadleaf Weeds in Corn with and without Roundup WeatherMAX<sup>®</sup>

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American Journal of Plant Sciences 2020年第3期11卷 465-495页

作者： Nicole M. Langdon Nader Soltani Alan J. Raedar Darren E. Robinson David C. Hooker Peter H. Sikkema University of Guelph Ridgetown Campus Ridgetown Canada ISK Biosciences Inc. Concord OH USA

Tolpyralate is a new HPPD-inhibiting herbicide that is efficacious on annual grass and broadleaf weed species in corn. For maximum herbicide performance of tolpyralate, it is recommended that atrazine is tank mixed with tolpyralate along with the adjuvants methylated seed oil concentrate (MSO) plus urea ammonia nitrate (UAN). A common use pattern of tolpyralate plus atrazine will be in a tank mix with Roundup WeatherMAX®due to the high proportion of corn acres that are seeded to Roundup Ready®hybrids in Eastern Canada. There is no information in the peer-reviewed literature if the adjuvant system in Roundup WeatherMAX®is adequate for optimal herbicide performance of tolpyralate plus atrazine, or if MSO and UAN are still required. Six field trials were conducted over two years near Ridgetown and Exeter, ON, Canada to determine if adjuvants are still required when tolpyralate plus atrazine is tank mixed with Roundup WeatherMAX®in corn. Tolpyralate plus atrazine plus MSO and Roundup WeatherMAX®plus tolpyralate plus atrazine provided excellent control of velvetleaf, pigweed spp, common ragweed, lambsquarters, ladysthumb, wild mustard, flower-of-an-hour, barnyardgrass and green foxtail in this study. Results of this study show that in the absence of Roundup WeatherMAX®, weed control with tolpyralate plus atrazine was improved substantially with the addition of MSO;however, there was little to no increase in weed control with the addition of UAN. When tolpyralate plus atrazine was co-applied with Roundup WeatherMAX®, there was no improvement in weed control with the addition of MSO and/or UAN.

关键词： Biomass Broadleaf Weeds Corn Efficacy Grassweeds Roundup WeatherMAX methylated Seed Oil Urea Ammonia Nitrate Weed Control Yield Zea mays L

Crystal structure of TDRD3 Tudor domain

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生物物理学报 2009年第S1期25卷 348-348页

作者： Danny Reinberg Howard Hughes Medical Institute and Department of Biochemistry New York University School of Medicine 522 First Avenue New York NY 10016 USA

The Tudor domain is a small, ～60 amino acid structure motif that serves to mediate intermolecular protein interactions. Recently, both structural and biochemical evidences

关键词： TDRD3 Tudor domain methylated arginine