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Biodegradation of methyl parathion by Acinetobacter radioresistens USTB-04

Journal of Environmental Sciences 2007年第10期19卷 1257-1260页

作者： LIU Fang-yao HONG Ming-zhang LIU Dan-mei LI Ya-wen SHOU Pei-shun YAN Hai SHI Guo-qing Department of Biological Science and Technology School of Applied Science University of Science and Technology Beijing Beijing 100083 China

Biodegradation of methyl parathion （MP）, a widely used organophosphorus pesticide, was investigated using a newly isolated bacterium strain Acinetobacter radioresistens USTB-04. MP at an initial concentration of 1200 mg/L could be totally biodegraded by A. radioresistens USTB-04 as the sole carbon source less than 4 d in the presence of phosphate and urea as phosphorus and nitrogen sources, respectively. Biodegradation of MP was also achieved using cell-free extract of A. radioresistens USTB-04. MP at an initial concentration of 130 mg/L was completely biodegraded in 2 h in the presence of cell-free extract with a protein concentration of 148.0 mg/L, which was increased with the increase of pH from 5.0 to 8.0. Contrary to published reports, no intermediate or final degradation metabolites of MP could be observed. Thus we suggest that the cleavage of C-C bond on the benzene ring other than P-O bond may be the biodegradation pathway of MP by A. radioresistens USTB-04.

关键词： Acinetobacter radioresistens USTB-04 biodegradation methyl parathion

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Facile purification and immobilization of organophosphorus hydrolase on protein-inorganic hybrid phosphate nanosheets

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Chinese Journal of Chemical Engineering 2023年第4期56卷 119-125页

作者： Zhenfu Wang Jie Gao Qinghong Shi Xiaoyan Dong Yan Sun Department of Biochemical Engineering School of Chemical Engineering and Technology and Key Laboratory of Systems Bioengineering and Frontiers Science Center for Synthetic Biology(Ministry of Education)Tianjin UniversityTianjin 300350China

Oriented immobilization of enzymes helps to maintain their native structure and proper orientation for high-performance engineering to meet extensive biocatalysis ***,the supporting materials used for orientated immobilization are usually costly or complicated in preparation,affecting their practical *** this work,a facile purification and immobilization method was proposed for enzyme immobilization based on organic-inorganic hybrid calcium phosphate nanocrystal(Ca Ps)induced by Cu^(2+) modified bovine serum albumin(BSA-Cu).Then,the as-prepared hybrid calcium phosphate nanosheet,BSA-Cu@Ca Ps,was utilized for one-pot purification and immobilization of His-tagged organophosphorus hydrolase(OPH)by metal-affinity binding to the incorporated ***-Cu@Ca PsOPH exhibited enhanced p H stability and thermal stability compared to the free ***,BSA-Cu@Ca Ps-OPH could retain more than 75%and 56%of initial activity after reuse 5 and 10 times,*** results demonstrated that this facile strategy was promising for the effective biodegradation of organophosphorus pesticides with the immobilized enzyme.

关键词： Calcium phosphate nanocrystal(CaPs) Biomimetic mineralization Organophosphorus hydrolase(OPH) Oriented immobilization Hydrolysis methyl parathion

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Metabolism-independent chemotaxis of Pseudomonas sp. strain WBC-3 toward aromatic compounds

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Journal of Environmental Sciences 2008年第10期20卷 1238-1242页

作者： ZHANG, Junjie XIN, Yufeng LIU, Hong WANG, Shujun ZHOU, Ningyi State Key Laboratory of Virology Wuhan Institute of Virology Chinese Academy of Sciences Wuhan 430071 China Graduate School Chinese Academy of Sciences Beijing 100049 China

Pseudomonas sp. strain WBC-3 utilized methyl parathion or para-nitrophenol (PNP) as the sole source of carbon, nitrogen, and energy, and methyl parathion hydrolase had been previously characterized. Its chemotactic behaviors to aromatics were investigated. The results indicated that strain WBC-3 was attracted to multiple aromatic compounds, including metabolizable or transformable substrates PNP, 4-nitrocatechol, and hydroquinone. Disruption of PNP catabolic genes had no e?ect on its chemotactic behaviors w...

关键词： aromatics chemotaxis metabolism-independent methyl parathion nitrophenol Pseudomonas sp. strain WBC-3

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